30-01-2020 дата публикации
Номер: WO2020022791A1
Автор:
PARK, Soon Jae,
CHUNG, Hye-Shin,
LEE, Seung Joo,
YOU, Sun-Ah,
SONG, Hyung-Nam,
LEE, Chang Woo
Принадлежит:
The present invention relates to a technical field of protein engineering for increasing the enzyme activity and thermal stability of human hyaluronidase, which is a hyaluronic acid-hydrolyzing enzyme, and relates to a hyaluronidase PH20 mutant or a fragment thereof, comprising: the substitution of at least one amino acid residue, among the amino acid sequence of the wild-type PH20 having SEQ ID NO: 1, at an alpha helix site and/or a site corresponding to a connection site thereof; and selectively, the additional deletion of an N-terminal amino acid residue and/or a C-terminal amino acid residue. Specifically, the present invention relates to a PH20 mutant and a fragment thereof, the wild-type PH20, having a sequence of SEQ ID NO: 1, comprising: the substitution of at least one residue selected from the group consisting of T341A, T341C, T341G, S343E, M345T, K349E, L353A, L354I, N356E and I361T; additionally, the substitution of an amino acid positioned at alpha helix site 8 and/or the connection ...
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