20-10-2016 дата публикации
Номер: US20160304887A1
The present invention relates to a female fertile variant strain of filamentous fungus derived from a female sterile parental strain which comprises at least one of the six female fertility specific gene alleles or derivatives thereof comprising the functional characteristics of these alleles (FS_4-9). 1. A female fertile variant strain of filamentous fungus derived from a parental female sterile strain comprising at least one of the gene alleles of SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:7 , SEQ ID NO:9 or SEQ ID NO:11 or a fragment or a derivative thereof.2. A female fertile filamentous fungal cell comprising at least one exogenous polynucleotide encoding a mating polypeptide comprising an amino acid sequence at least 60% identical to a sequence chosen from the group of sequences consisting of SEQ ID NO:2 , SEQ ID NO:4 , SEQ ID NO:6 , SEQ ID NO:8 , SEQ ID NO:10 and SEQ ID NO:12; preferably the amino acid sequence is at least 65% identical , more preferably it is at least 65% , 70% , 75% , 80% , 85% , 90% , 95% , 97% , 98% , 99% or 100% identical to SEQ ID NO:2 , SEQ ID NO:4 , SEQ ID NO:6 , SEQ ID NO:8 , SEQ ID NO:10 or SEQ ID NO:12.3. A female fertile filamentous fungal cell comprising at least one exogenous polynucleotide , wherein the cDNA of which having a nucleotide sequence at least 60% identical to the joined coding region(s) in SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:7 , SEQ ID NO:9 or SEQ ID NO:11; preferably the nucleotide sequence is at least 65% , 70% , 75% , 80% , 85% , 90% , 95% , 97% , 98% , 99% or 100% identical to the joined coding region(s) in SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:7 , SEQ ID NO:9 or SEQ ID NO:11.4. A female fertile filamentous fungal cell comprising at least one exogenous polynucleotide having a nucleotide sequence at least 60% identical to SEQ ID NO:1 , SEQ ID NO:3 , SEQ ID NO:5 , SEQ ID NO:7 , SEQ ID NO:9 or SEQ ID NO:11; preferably the nucleotide sequence is at least 65% , 70% , 75% , 80% , ...
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