14-11-2013 дата публикации
Номер: US20130302313A1
Принадлежит:
The invention provides a hexon Tat-PTD modified adenovirus, a gene delivery vector based on the modified adenovirus that enhances gene delivery efficiency, and an oncolytic agent based on the modified adenovirus that enhances tumor cell killing efficiency and improves therapeutic outcome. 1. Adenovirus comprising at least one of (i) an adenovirus hexon protein which is modified to comprise at least one protein transduction domain of the Tat protein from Human Immunodeficiency Virus (HIV) and (ii) a recombinant nucleic acid molecule encoding the said modified hexon protein.2. Adenovirus according to claim 1 , wherein said HIV is HIV-1.3. Adenovirus according to claim 1 , wherein the adenovirus hexon protein is modified to comprise the protein transduction domain within a hypervariable region claim 1 , such as HVR5.4. Adenovirus according to claim 1 , wherein said protein comprises the amino acid sequence YGRKKRRQRRR (SEQ ID NO: 1).5. Adenovirus according to claim 1 , wherein said protein comprises the amino acid sequence AGGGAGGGYGRKKRRQRRRGGGAGGGA (SEQ ID NO: 2).6. Adenovirus according to claim 1 , wherein said protein comprises the amino acid sequence according to SEQ ID NO: 3.7. The adenovirus of claim 1 , wherein the adenovirus is selected from other serotypes than human adenovirus serotype 5 (Ad5) including serotype-2 (Ad2) claim 1 , -3 (Ad3) claim 1 , -7(Ad7) claim 1 , -11 (Ad11) claim 1 , -17 (Ad17) claim 1 , -35 (Ad35) claim 1 , -41 (Ad41) claim 1 , -48 (Ad48) and their derivatives.8. The adenovirus of claim 1 , wherein the adenovirus is an adenovirus serotype 5 comprising the fiber and/or fiber knob of other adenovirus serotypes claim 1 , in place of the adenovirus serotype 5 fiber and/or fiber knob.9. The adenovirus of claim 8 , comprising the fiber and/or fiber knob of adenovirus serotypes selected from serotypes Ad2 claim 8 , Ad3 claim 8 , Ad7 claim 8 , Ad11 claim 8 , Ad17 claim 8 , Ad35 claim 8 , Ad41 and Ad48.10. The adenovirus of claim 1 , wherein the ...
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