MODIFIED $i(E. COLI) ENTEROTOXIN II SIGNAL PEPTIDE AND A MICROORGANISM EXPRESSING A FUSION PROTEIN OF SAID PEPTIDE AND A HETEROLOGOUS PROTEIN
Номер патента: EP1114062A1
Опубликовано: 11-07-2001
Автор(ы): Gwan Sun Lee, Hoon Shin, Jay Do Choi, Ki Doo Choi, Se Chang Kwon, Sung Youb Jung
Принадлежит: Hanmi Pharmaceutical Co Ltd, Hanmi Pharmaceutical Industries Co Ltd
Опубликовано: 11-07-2001
Автор(ы): Gwan Sun Lee, Hoon Shin, Jay Do Choi, Ki Doo Choi, Se Chang Kwon, Sung Youb Jung
Принадлежит: Hanmi Pharmaceutical Co Ltd, Hanmi Pharmaceutical Industries Co Ltd
Реферат: A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified E. coli enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, said modified E. coli enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th and 22nd amino acids of E. coli enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm.
New version of efflux protein of o-phosphoserine and method for producing o-phosphoserine, cysteine and its derivative using this embodiment
Номер патента: RU2694589C1. Автор: Мин Гён КАН,Соль КИМ,Ин Хва Ю,Хе Вон КИМ,Чжон Хён КИМ. Владелец: СиДжей ЧеилДжеданг Корпорейшн. Дата публикации: 2019-07-16.