Non-terminal antibody discovery methods and single cell assays
Номер патента: EP4288524A2
Опубликовано: 13-12-2023
Автор(ы): Aaron George Winters, Brian M. CHAN, Christopher Murawsky, Daniel Santos, Karyn E. MCFADDEN
Принадлежит: AMGEN INC
Опубликовано: 13-12-2023
Автор(ы): Aaron George Winters, Brian M. CHAN, Christopher Murawsky, Daniel Santos, Karyn E. MCFADDEN
Принадлежит: AMGEN INC
Реферат: Provided herein are methods of monitoring for the production of select antibodies in a non-human animal, comprising (a) immunizing a non-human animal with an immunogen; (b) obtaining a blood sample comprising antibody secreting cells (ASCs) from said non-human animal; and (c) individually assaying ASCs present in the blood sample, or a fraction thereof, for the production of select antibodies. Methods of guiding antibody production in a non-human animal for the production of select antibodies are also provided. In exemplary embodiments, the method comprises performing a cycle of (a) to (c), as above, and repeating the cycle when the percentage of ASCs producing select antibodies is below a threshold. In various aspects, the cycle is repeated until the percentage of ASCs producing select antibodies is at or above a threshold. Single cell assays are further provided herein.
Non-terminal antibody discovery methods and single cell assays
Номер патента: CA3210091A1. Автор: Aaron George Winters,Christopher Murawsky,Daniel Santos,Karyn E. MCFADDEN,Brian M. CHAN. Владелец: AMGEN INC. Дата публикации: 2022-08-11.