Cell fusion assays for the identification of antiviral compounds, and systems and kits for practicing the same
Номер патента: EP1390469A2
Опубликовано: 25-02-2004
Автор(ы): Mark A. Goldsmith, Yun You
Принадлежит: J David Gladstone Institutes
Опубликовано: 25-02-2004
Автор(ы): Mark A. Goldsmith, Yun You
Принадлежит: J David Gladstone Institutes
Реферат: Cell fusion assays for identifying antiviral compounds are provided. In the cell fusion assays of the subject invention, a first cell that stably expresses on its surface an envelope protein of an enveloped virion and a second cell that stably expresses a receptor for the envelope protein on its surface are employed. The first and second cells each contain one component of a two component Tat reporter system that produces a detectable product in the presence of cell fusion. In practicing the subject screening methods, the first and second cells are first contacted with each other under cell fusion conditions in the presence of a candidate inhibitory agent. Next, the presence or absence of the detectable product is detected. Finally, the inhibitory activity of the candidate agent is derived from the presence or absence of the detectable product. Also provided are high throughput embodiments of the subject methods. In addition to the subject methods, systems and kits for performing the subject methods are provided.
A Process for Preparing Seed Powder Matrix Reference Materials for Detection and Identification of CGMMV
Номер патента: LU502712B1. Автор: Yi Zhang,Yiling Xu,Chao Ma,Jijuan CAO,Zhongting Lu,Xinying Yin,Yiluan Liu,Shuang Yan. Владелец: Univ Dalian Minzu. Дата публикации: 2023-02-27.