Ms/ms-based identification of trisulfide bonds
Номер патента: EP4405687A1
Опубликовано: 31-07-2024
Автор(ы): Yuzhuo Zhang
Принадлежит: DH TECHNOLOGIES DEVELOPMENT PTE LTD
Опубликовано: 31-07-2024
Автор(ы): Yuzhuo Zhang
Принадлежит: DH TECHNOLOGIES DEVELOPMENT PTE LTD
Реферат: Methods and system that determines disulfide and trisulfide linkages within analytes (e.g., polypeptides) is described. In certain aspects, a sample comprising polypeptides (such as an antibody) may be subjected to dissociation using an electron activated dissociation (which can include electron capture dissociation and electron transfer dissociation) and the fragmentated portions are analyzed using a mass spectrometer to produce a spectrum. The spectrum is analyzed by a processor to identify peaks from the spectrum that are related to one another in the spectra by a separation of 32 mass units. In identifying an antibody comprising peptide segments linked via a trisulfide bond, for example, four different peaks representing two different peptides are searched for and identified representing a first peptide portion having mass/charge of A and A+32 and a second peptide having mass/charge of B and B+32.
Methods for separation, characterization and/or identification of microorganisms using spectroscopy
Номер патента: US09790534B2. Автор: John Walsh,Thurman Thorpe,Bradford Clay,Jones Hyman,Christopher Ronsick. Владелец: Biomerieux Inc. Дата публикации: 2017-10-17.