Pharmaceutical compositions having anti-metastasis activity comprising extract of bambusae caulis in taeniam
The present invention refers to extract the kill including metastasis a pharmaceutical composition and is provided to inhibit metastasis is directed to a food composition for inhibiting.. Arm for viewing and mortality high world which, in social roasted nutty then cardiovascular disease is cause death the most general. In particular, increase in population smoking with quickening of population, contamination on and stand-by mode the is lung cancer, eating habits is ingestion of type fat is roasted nutty taste and and generalized is, environmental contamination abrupt increase in a, colon or the like increase in sound drinking capacity , breast cancer, prostate cancer and the like. and total RNAs of kgmmv are continues to increase and. Such regularized data in a database earln of cancer in human permit treatment prevention and enhancement of health, in healthy life of for promoting health man and be which a component can contribute to the anticancer agents is the MTS. A long period of time of not when a user of a major portion of the malignant tumor (waste, liver, elongated, on, bowel, such as rectal) after a reaction has occurred in the initially generated a primary region of the other tissue from an organ, which spreading, thus primary clutch and the other tissue from the main body portion the pressure is reduced to the transition leak outside and carbonic acid gas in:a buffer (metastasis). Translation with a with the progress of a malignant tumor, malignant tumor cells tumor imbalance transition progresses the required new dielectric traits which is moistened lymph line with a blood vessel after obtaining the limp and the blood using only one cyclically along after anchoring in other tissues is proliferation. Recent research the transition metal upon heating in a dielectric associated with which such that the are transgenic, through primary gene of tissue through transition to later other long period of timehigh risk recurrence is enabled personalized based on group. Such transition in an initial step protease matrix metalloproteinase (MMP, matrix metalloproteinase) extracellular matrix and the sensitize decomposition base film s to inhibit cancer cell infiltration of important in order to transition inducing serve to a separation at least one inert gas 20 until now, been found. The MMP using enzyme auxiliary zinc (endoproteinase) to to pro mote recovering sacrifice , artificial marble enzyme (collagenase), gelatin degrading enzymes (gelatinase), capsaicin (stromelysins) bromelaine strobe, Membrane-type MMP are divided into. In particular, these MMP MMP-2, among other things, (72 kDa type IV collagenase; gelatinase A) and a MMP-9 (92 kDa type IV collagenase; gelatinase B) 2001 component the basement membrane enzyme for decomposing an aromatic Type IV collagen, most directly transitions and moving of the cancer associated recurrence is correlated with increased and mortality are known as HMG-COa reductase (Nabeshima, K. Et al. , To date cancer given either systemically medicaments is developed for an injection or peroral drug for the treatment of whole along the blood flow as [...] acts in the manner of non-specific low specific to cancer, pharmaceutical administration according to systemic side effects or radiation therapy for cancer surgery has taken n is the fixed number more than compared is connected to the semiconductor layer. S100 much adverse. Cancer cells directly attack the existing chemical with gas and sealed immunoprophylactic standard is simplified and only the steps also side effects, such as transition to an long-term such lung a multiplexer are the new drug has been acclaimed the objects' traces to not. The, the present inventor transition without causing side effects into a body are the prevention of therapy natural extract example effort results classified by sex, isolated from bamboo extract is effective in preventing the kill within such a range that is represented by transition an article on a that, he rattled through his the present invention. The present purpose of the invention is provided to inhibit metastasis including the kill the extract by a rope. medicinal compositions for. The kill another object of the present invention extract for inhibiting tumor metastasis including electrode 104 is provided under the foodstuff composition. Another object of the present invention administered to the subject extract the kill cancer metastasis, or by a rope. provides a method. said callee opens the folder of his in one aspect, the present invention refers to inhibitors of transition including extract the kill provides a pharmaceutical composition for.. In the present invention, "(bambusae caulis in taeniam, [...]) the kill" after removing contaminants and metal oxides in the shells of bamboo has [...] layer fiber numeral key, shines of sonar as is with the threads which from is inside shell device, a. At least a fragment film 111,113 features irregular shape or drilling, notably by and a thread-like shape in width and thickness are inclined at a outside not powder and green sides of the channel region is. A light have to be resilient, soft, and does not crumble easily are fibrous which. [...] other name as a keyword, gruel blood , wall gruel blood woman bonded. Yellow is shovelgruel woman sea water due with waste heat is adhesively a treating, at wall ten walls and come together in the function on heart to unbalance is reduced for a transient operation and returned to the above stage, the sweep and prevent Lullaby shovel the uniform sintered body target has atmospheric pressure thrusts the sewage in a path a vomiting, in up emptiness and up ten due vomiting, hiccup, pregnancy vomiting medicament has into alcohol; adding 0.001-0.1M and can be effective even in.. Fetal is configured to snugly the which, pharmacological effect white [...] , ten germs subtilis, e. coli, bacteria [...]. action potent inhibitors or the like. gruel woman neurological vomiting, the recurrence relation of the generated by selecting and inserting a name sweat pulmonary tuberculosis , acute heterogeneous, face neuroinflammation, pediatric bronchitis , oh! sacrifice (child night when troubled conditions wool in stroke and), energy phosphoanhydride a 100,000. efficacious effect or the like. Plant extraction site activity of fraction extracts or their each according to whether, degree of active, and to the respectively. may be different from the user component. outer cover of long bamboo the present invention refers to a fibre-of concent transitioning of the extract the kill preventing or therapeutic uses thereof. found to initially. The kill of the present invention. is known to effectively inhibit a transition extract. In the present invention to sold at the commercial gruel woman or purchase, cultivated or sampling in natural, use can be made of, to. outer cover of long bamboo extract the kill of the present invention after removing contaminants and metal oxides in distilled ground fibrous inner surface of (D. W.), 1 to 6 of carbon number alcohol. may be obtained by extraction. Kill method for producing the thermal extraction method extraction such as conventional homogeneously distributed, use can be made of,. Preferably after removing contaminants and metal oxides in outer cover of long bamboo inner surface of fiber washing and drying furnace removes the foreign substance, gruel woman has an excellent safety building with water, distilled (D. W.), C1-C6 solvent or a combination of alcohol is, more preferably C1-C4 of alcohol or distilled (D. W.) and capable of extracting, most preferably distilled (D. W.). capable of extracting. The, of a dry weight of the kill extraction solvent is 2-20 it is preferable that the electrode is formed on a first. gruel woman , for example, the powder paste has better mouth feeling and extraction vessel after mixing distilled, or C1 -C4 alcohol or a combination of solvent, preferably distilled (D. W.) for inserting and removing distilled 38-120 °C extracts distribution information (D. W.) can cause extract. The, such as concentrated or lyophilized after extraction method, in one embodiment, are. therefore, it has improved productivity. In the present invention, "tumor metastasis" encodes a long-term developing malignant tumor email widow, a web page or the substrate from the heater and an and the other tissue propagate into the. in that a line. One organs, start of malignant tumor progresses the first generated a primary region of the other tissue from an organ, which spreading, and the other tissue from the primary thus leak outside and carbonic acid gas in clutch can be the pressure is reduced to the transition position of a reflector according to each. Translation with the progress of a malignant tumor with a second the composition can be taken 2-which, imbalance malignant tumor cells tumor progresses transition while obtain a new dielectric traits. capable of. New dielectric traits obtained with a blood vessel tumour cells will lymph line invasive along limp and the blood and the other tissue anchoring in using only one cyclically, and thus. capable of plane transitions. According to a transition occurs tissue, hepatocellular carcinoma, agent of kidney cancer, lung cancer, stomach, colon cancer, colon, or pancreatic. tolerance can be induced histone deacetylase. Cancer metastasis, or composition of the present invention is to prevent and treat the microorganism infection from spreading as well as, transition derived from improved dark related disorders, prevention, can be treating. Terms in the present invention, the present invention according to the "inhibitors" or metastasis tumor metastasis said by administration composition from cancer associated of developing diseases deriving a all which is effective to suppress the circulation promoted. behavior. Terms in the present invention, the present invention according to the "preventing" said by administration composition from cancer associated deriving a metastasis or metastasis of suppressing or of developing diseases circulation promoted. behavior all delay. Terms in the present invention, the present invention according to the "treatment" or metastasis tumor metastasis said by administration composition from cancer associated deriving a presenting the symptoms of diseases or improving circulation promoted. behavior all the beneficial. only simultaneous pharmaceutical compositions of the present invention can be coating the parabolic antenna with chromium, known having an effect-metastasis, anti-certified pharmaceutical compositions complex the further include an, use can be made of, the. Pharmaceutical compositions of the present invention the pharmaceutically acceptable carrier, excipient number, the work by adding an additional or diluent, formulated pharmaceutical a unit dosage of a penetration hole while moving up and down. "Pharmaceutically acceptable" said rotation significantly biological early warning system or dispensing not stimulating biological activity of active substances does not detract from the and characterization. provide the means by which. In the present invention including said carrier pharmaceutically acceptable oral or parenteral composition can be a variety of formulations. Formulated filling common when the number, number extender, coupled number, wetting, disintegrating, or of a thinning agent surfactant is formulated excipient. Solid medicinal preparation is finished, the perfume ingredient for oral administration, bolus, masked powders that are to be, granules number, capsule number including an, such solid formulation contains at least one compound at least one or more excipients number for example, starch, calcium carbonate, sucrose (sucrose) or lactose (lactose), is prepared of gelatin. In addition to excipients in addition simple magnesium stearic acid number, such as talc is used lubricants provide. Liquid formulations for parenteral administration include suspension number, content liquid, emulsion, syrup number, including the additive and water as the diluent a simple commonly used which, in addition to paraffin a bioliquid number various excipient, for example wetting, sweetener, aromatic, and preservatives may include. Agents are for parenteral administration in the sterilized aqueous solution, non-aqueous solvent, number suspension, emulsion, freeze-dried preparation, left system may be included. Non-aqueous solvent, suspension solvent include propylene glycol (propylene glycol), polyethylene glycol, vegetable oil olive, such as an oil, ethyl [...] such as injectable esterase can be is used furnace, such as a flash. [...] include base left proposal (witepsol), macrogol glyceride, twin (tween) 61, carcass five fingers, does comprising lauric acid, glycero gelatin can be or the like is used as an. Said purified pharmaceutical compositions, bolus, masked powders that are to be, granules number, number capsule, suspension number, content liquid, emulsion, syrup number, in the sterilized aqueous solution, non-aqueous solvent, number suspension, emulsion, selected from the group consisting of freeze-dried preparation and left zero be decreased may have formulations of. Furthermore, in the composition of the present invention the kill a pharmaceutically effective amounts of extract can be. Terms in the present invention "in a pharmaceutically effective amount and a" applicable to medical treatment through a rational will benefit/risk ratio a unit, device and method to a sufficient amount meaning, object types and the level capacity effective in the severity, age, sex, drug action, sensitivity to drugs, administration time, route, and discharge ratio, therapy period, the inhaler includes a mechanism to open signal by using an elements and other medical well-known to the field can be determined according to factors. Useful when administered therapeutic agent individual composition of the present invention or with additional therapeutic agents can be administered from therapeutic agent of the existing method can be sequential or simultaneous administration. Single or multiple and. can be administered. Said least without adverse considers both element a starting switch enables the maximum amount of administration of a amount are important, by one skilled in the art can be the storing apparatus. Preferably in the present invention a composition extract the kill 0.001 to 50 weight % may be included in an, more preferably 0.001 to 20 weight body is included to the display apparatus %. In of the present invention in the embodiment, which comprises the steps of extract the kill, characterized of a malignant tumor attachment-dependent and attachment-independent colony by of analysis results of forming (colony), is effective in preventing the kill at a concentration in the cells to a type used in extract and compared to the control group when groups of cells HT1080 adhesion-dependent and attachment-independent growth can be a high resolution PSD so that the both can be acyl. In another of the present invention in the embodiment in the case of the group the extract and the kill, concentration dependent fashion compared to the control group in the kill is suppressed and hence ability mobile cancer-metastasis, anti-extract can be acyl can be. Another in the embodiment of the present invention in vitro in cell migration and well transport infiltration royal tomb cells in assays, cell migration and the kill active cell infiltration in cells-treated extract significantly significant corresponding advertisement based on the shown list reduced, thus the kill movement and invasion inhibitor gene of a cell extract-metastasis, anti-through can be acyl can be. In another of the present invention in the embodiment to destroy the cells that are HT1080 to preprocess the extract (FN) fibronectin when the attachment to the collagen and method-dependent capacity capacity been reduced, extracellular matrix (ECM) to preprocess extract the beetle when the start of cells did not capabilities attached. The kill the extract are tumor surrounding tissue without affecting the attached which acts directly on cancer suppressed ability can be verify that the. Another in the embodiment of the present invention in the extracellular matrix (ECM) is decomposed to thereby tumor metastasis plays an integral role in the expression and MMP-9 that are known to kill activity relates to efficacy of the extract, the kill by extract and pads by PMA MMP-9 activity and expression amount by dramatically reduced. Another in the embodiment of the present invention in in cells-treated extract the kill, pads by PMA molecular weight IκB phosphorylation and p65 as a result been suppressed mobile position in nuclear protein is suppressed was also significant. Furthermore, induced stimulus PMA extract the kill p38 significant phosphorylation of ERK1/2 and by a medium to generate a voltage. Another in the embodiment of the present invention extract the kill in PMA derived stimulus inhibit ROS production, subsequent NF-κB MMP-9 activity and for inhibiting the activity of by. In another of the present invention in the embodiment the kill C57BL/6J extract through blood vessels by oral administration to a mouse the implanted B16F10 waste cell in vivo metastatic colony proliferation of significantly reduce the propagation-absorbability/transition waste maintain the in-vivo it is found out that to have undergone an aspect significantly inhibiting. In particular, light between the laser beam and the kill teleservice extract an infusion administration compared to the control group group GOT/GPT and BUN/CRE ratio, numeral and of red blood cells and have not been changing significant hemoglobin levels, leukocyte number, other parameter in the range to an event that both a normal extract the kill since oral route of administration is in vivo to identifying improving without showing any side effects. As such, without adverse effect extract the kill from or metastasis is provided to inhibit metastasis prevention and treatment of diseases cancer associated deriving a chamber and outputs an electrical signal can be confirm that the user. In other embodiments, the present invention refers to kill the extract from or metastasis is provided to inhibit metastasis pharmaceutical composition including deriving a cancer associated the prevention or the treatment of disorders to a subject in need of a cancer by from or metastasis metastasis, or cancer associated deriving a method for preventing or treating diseases provides. Said tumor metastasis or metastasis from cancer associated diseases deriving a hepatocellular carcinoma, lung, stomach, colon cancer, colon can be various kinds of cancerous disease, not limited to blood. Said entity is provided to inhibit metastasis or metastasis cancer associated deriving a from the prevention or the treatment of disorders as a subject in need of a, as well as tumor metastasis or metastasis from human cancer associated deriving a disease and its underlying conditions of the symptom similar to a saw in need of such treatment a, horse, amount, porcine, chlorine, camel, nutritional, two, can be of mammalian such as CAT, are not limited to. Terms in the present invention, "administering" the upon any suitable method to the patient as either pharmaceutical composition of the present invention introducing meaning that it, the aim path treatment of tissue of the present invention which can be reached the oral or parenteral of the various pathway through. can be administered. Of the present invention is provided to inhibit metastasis or metastasis method the treatment of diseases associated with cancers derived from the kill pharmaceutical by administering an effective amount of the extract as to. 1 total suitable medical the correct usage in justice determined by from a within range-can be to one skilled in the art is scrapped. Furthermore, administration 1 times once or can be divided into. Outputted on purpose of the invention, particular patient the therapeutic amount is effective degree, the types of reaction portions are mounted on a central, optionally other used including whether the second time control composition, patient's age, weight, general health status, gender, and dietary, administration time, route, and compositions secretion rate, therapy period, or use with compositions specifically the inhaler simultaneous use of a variety of factors including and medical field to well-known like factor device includes a rear substrate preferably applied. As another embodiment of the invention, including the present invention refers to kill the extract provides a food composition for inhibiting tumor metastasis. With regard to extract the kill said said taught equal. Said food composition transition, or said transition described used for reduction in generation of related disorders can take the function. Food composition of the present invention for inhibiting the bolus, powder, granules, infused with, purification, includes form of capsule or liquid, food the composition is added to the material of the present invention at, for example, various voltage white, for example, beverage, gum, difference, vitamin complex, such as voltage white health. Said food composition addition extract the kill-metastasis, anti-active and it is not to obstruct the other ingredients that can be adding, gelling agent in various applications is not limited especially. For example, various medicinal herb extract, such as conventional food, a food supplement a dermatologically acceptable food additive or natural carbohydrate furthermore, the number of as a component can be. Terms in the present invention auxiliary food "food additive" email widow, a web page or component of which may be added by the addition of an initiator, each of the dosage form healthy food as added to the low target value in response can be appropriately selected is one skilled in the art, use can be made of, the. Examples of a food supplement additive various nutritional number, vitamin, mineral (electrolyte), synthetic flavor number and natural flavor number flavor such as number, number and filling number colored, [...] and salts thereof, alginate and salts thereof, organic acid, protective colloidal thickener, pH modulators, stabilizer, preservative, glycerin, alcohol, such as agent carbonate used carbonated beverage including, but, a food supplement of the present invention by examples said type of additive the not is promoted and the limit. Examples of carbohydrate natural said mono waste transfer crane of nuclear power, for example, glucose, such as oligosaccharides; disaccharides, maltose for example, such as sucrose; and polysaccharide, for example dextrin, cyclodextrin and the like is conventional processing modules, such as sugar, and xylitol, sorbitol, erythro [...] is sugar alcohol such as. The above-mentioned other than natural flavor number number flavor (TAU Martin, Stevia extract (A [...] for example, such as [...]) and synthetic flavor number (saccharin, such as [...]), use can be made of, advantageously. Health the food composition of the present invention can be includes as a functional food. In the present invention terms "health functional food" functional corn syrup, rice bran, rotation or components raw material with purified using, capsule, powder, granules, manufacturing and machining for example, in such a form ring liquid and food. of the mobile communication network. Wherein functional addition, the height of of a human body nutrient to structural and functional physiological the handle, such as activity health which is useful for application effect.. Health of the present invention typically used in the art for the functional food producible by the which a method, in the art for active material is formed on said to add to conventional and materials component is added to precipitate out can be produced. In addition drug generally food unlike long-lasting administration in order to/or utilisation of various agents, to the exterior of body side effects the semiconductor substrate having the insulation layer is a free, of this can be excellent. A use purpose tilted mixing of active ingredient (prevention, health or therapeutic treatment of) according to can be determined for. Generally, the kill of the present invention in the production of food extract raw composition 1-10 weight %, preferably 5-10% is added amount of weight. However health and sanitary pad intended to a long is intended to control health or in the case of ingestion of the amount said said range can be used as hereinafter. With specific type of food said't limitation. With addition of said examples of food meat, sausage, bread, chocolate, candy current, [...] , confectionery, pizza, ramyon, other noodles, gum type , ice cream containing a dairy product, various sprocket, beverage, difference, abundant, alcoholic drinks and vitamin complex is to, in the conventional health food includes both. As another embodiment of the invention, the present invention refers to (a) water, distilled, C1 -C6 alcohol, or a combination of vehicle speed is lower than the extracted gruel woman solvent, and filtering extract obtained at the step (b) said (a), including concentrating and, the kill provides method for producing. Preferably, using selected depending gruel woman extraction solvent can cause extract the kill. Extract the kill of the present invention including pharmaceutical compositions, capable of having-metastasis, anti-low cytotoxic and is matrices have plural polypropylene matrix fabric the advantage of without showing any side effects, can be a tumor metastasis, is provided to inhibit metastasis number, is provided to inhibit metastasis or functional food for useful, use can be made of, boronic acids. The MTT analysis (assay) through 1a also cell survival rate is graph for showing evaluation result. Also 1b through the LDH emission analysis (LDH release assay) cytotoxic result is graph for showing. The HT1080 cells also 1c and 1d, which divided the lower density is effective in preventing extract the kill after a 50-250 micro g/Ml concentration colony forming-dependent attached while processing is inhibited is a shown that drawing. The 1f and 1e also is effective in preventing the kill of a 50-250 micro g/Ml extract processing at concentrations, as compared to untreated the control group cells adhesion-independent of (semi-manner in a medium free of ability of colony cells forming) about 40-97% significant degree a shown that a medium to generate a voltage is drawing. Also 2a and 2b has an in vitro kill anti-transition efficacy inspection one of the extract, cell motility (wound healing assays) analysis wound healing for observing result is drawing for showing. The 2d and 2c also concentration of extract the kill according to cell migration and well transport active cell infiltration as drawing for showing analysis result, the preparation of the extract the kill of 50-250 micro g/Ml the control group pending access requests in favor of a degree approximately 30-50% as compared to cells cell migration and cell infiltration to hold on to his temper-dependent capacity activity. The fibronectin (FN) also 2e and 2f for-type collagen I and attached of cells HT1080 the kill capacity drawing as a effecting extract, the extract to destroy the cells that are HT1080 to preprocess (FN) fibronectin when the attachment to the collagen and method-dependent capacity capacity discharged from the discharge circuit is reduced. The 3a also extract the kill MMP-9 are capable of modulating the activity whether an gelatin graphitic wool yes in blood inspected by drawing for showing results is. MMP-9 extract the kill the 3b also regulates the expression whether as a result of the inspecting [...] blots, PMA processing extract the kill of 50-250 micro g/Ml pads by increased expression of activity and MMP-9 a capacity-dependent method in dramatically reduced. The PMA also 4a IκBα phosphorylation increase and pads by IκBα decomposition compared to the control group increase the kill cells HT1080-treated extract below the predominantly in is drawing a. The 4b also in cells-treated extract the kill, nuclear protein p65 pads by PMA position in significant compared to the control group movement is suppressed shown that is a drawing. Figure 5 shows a also kill the extract of PMA ERK1/2 pads by phosphorylation of significant a medium to generate a voltage. surface thereof, and the shown that. The HT1080 6d also to 6a also PMA pads by ROS for increasing production in cells for the kill drawing as a effecting extract, extract the kill-induced cell stimulation PMA -70% in to synchronize the reduced by ROS production, ROS production inhibitory through MMP-9 activity and κB-NF for inhibiting the activity of by. Also the 7c also to 7a the kill administering saline when by administering the test extract the control group compared to significant colony is cancer metastatic to the lung frame a is drawing. Hereinafter, embodiment to the present invention. described S406. These embodiment relate more specifically, the present invention for, embodiment of the present invention range not limited aspect. < 실시예 1>Extract the kill (AE-BCT) for manufacturing A dry provided commercially (bambusae caulis in taeniam extract, BCT) the kill yongch'on a market or other nutriments to the (yongch'on , 25th which) for purchase in corresponding advertisement based on the shown list, kill the extract for preparing a (aqueous extract of bambusae caulis in taeniam, AE-BCT) the, gruel woman dried portion (50.0g) is placed in the distilled water of 1 L of, extractor (course Morse -600 extractor, industrial machine chinese classics , Incheon, 25th which) in 3 to 115 °C extracted heat time. Sheave standard test extract the kill (body) (150 micro m, Retsch, Han, Germany) by the filter by using it is under, , freezing dryer (lyophilizer) was concentrating until dried in. A kill freeze-drying the extract powder (50 mg) of a massage cream, an essence, melting to distilled water of 1 ml, 0.22 micro m of disk filter (disk filter) through the filter it is under, , was stored in a -20 ° C until prior to use. < 실시예 2>Cytotoxicity assay: MTT and LDH emission analysis HT1080 cells for assessing the toxicity the, MTT and LDH emission analysis (MTT and LDH release assay) on an entire surface of the semiconductor, HT1080 cells (5x103 one cell/well/96-well plate) a concentration of between 10 and 250 micro g/Ml the incubating the sample with extract the kill. 48 after the processing time, cells of 10 micro l MTT solution (5 mg/Ml in PBS) in the incubation additionally hours' extra 4. Formazan (formazan) precipitate Dimethylsulphoxide (dimethyl sulfoxide, DMSO) senses a rotation velocity of the disk to, Infinite R M200 absorbance microplate reader (TECAN Group Ltd, a synchronized switch) measured at 570 nm to. Culture supernatants as well as from cells and-processing extract the kill LDH release to manual guide manufacturer according to conventional cytotoxic chemotherapies commercial detection kit decided to get out of. Also decodes a as the control group nothing control 1a and 1b as compared to cells of concentration of 25-250 micro g/Ml the kill rate cell survival when treated extract, without affecting the emission LDH people, Letters and the thing which it is found out that a toxicity. Therefore, in the present invention 25-250 micro g/Ml the extract concentration range of kill at the ground terminal of a process consisting of two adaptation steps. < 실시예 3>Attach-dependent and attachment-independent colony forminganalysis <3-1>Attach-dependent colony forming analysis HT1080 cells as that of the aluminum or a attachable, 200 in 1 ml of 10% FBS/DMEM of HT1080 cells (seeded) was frequency divides the 12 well culture plate. The kill micro 50-250 g/Ml after treating the extract, the n bit parallel data inputted incubation days 7-10 cells, generated colony 0.2% crystal violet (crystal violet)/ 20% methanol (w/v) solution to dye of 100. <3-2>Colony forming dependent non-adherent analysis Attach-independent to make sure that cell growth, cells (5 x 104 two) extract the kill of a specific concentration a, 0.3% agar, and including 10% FBS it makes, supply device of index suspended in 2 ml, 0.6% agar and same to the coagulation is included 10% FBS is applied to the agar bottom after, during incubation attention 2, the smoothly microscope phase difference agar are observed to colony formed in a electrophotographic he jabbed his. <3-3>The kill at concentrations which is effective in preventing the (AE-BCT) extract of cells HT1080 adhesion-dependent and attachment-independent growth of the than the size of the gate hole both In lower density cells after the kill HT1080 at concentrations non-cytotoxic and is extract of cells to colony forming-dependent attached can adversely effect sensors whether an. Kill the extract and in a fast cells HT1080 with the control group 1 having no shows an, single cell from substantial are formed at the are colonization. While, when extract and the kill incubation during concentration dependent method transmits the activity colony in, been significantly smaller colony is of apertures with an effective size greater, colony number of it attached dependent growth to suppress the (also 1c, 1d also). Extract the kill in addition, at concentrations of 50-250 micro g/Ml, untreated as compared to the control group cells adhesion-independent of (semi-manner in a medium free of ability of colony cells forming) significant degree about 40-97% was a medium to generate a voltage (also 1e, 1f also). < 실시예 4>Cell migration capabilities analysis Cell motility order to monitor the wound healing (wound healing assays) is performed for all the analysis. About 80% to the pathogenic cells which propagates to the single layer degree 25 micro g/Ml of erythromycin mitogenic C (mitomycin C) (Sigma chemical Co.) to 30 minutes after in arresting the proliferation cells processing, single layer is performed for damage (wound) and went about 2 mm degree. Cells away followed by reducing the completely floating things, includes extract the kill culture media during a period of weeks after layer is wet-ability cell migration at the site of injury has been observed on using the phase difference. Also 2a, 2b also as if it were on the, untreated the control group HT1080 cells 36 extending into the hollow interior for permitting healing damaged parts of time was about 50%. Kill the extract and the cells to a type used in group as compared to the control group cells approximately 60-90% concentration dependent fashion damaging force applied to the target fastening portion mobile has been inhibited by the. < 실시예 5>In vitro cell migration and impregnated ability analysis 8 polycarbonate sized polysilicon micro m hole (pore) (Corning costar, Cambridge, MA) (polycarbonate) film with well chamber of diameter 10 mm using (transwell chamber), cell migration and impregnated analysis (migration and invasion assays) is performed for all the treatment of osteoporosis. Unloaded over surface, 10% FBS/DMEM 600 micro l downward chamber (lower chamber) of a massage cream, an essence, fill, serum-free DMEM 100 micro l and cell extract the kill to (1 x 105 micro l open/100) for inserting and removing each above chamber (upper chamber) after the incubation in 37 °C placed in. The filter has not moved by a the upper surface remaining in the removing and, filter 0.2% crystal violet (crystal violet)/ 20% methanol (w/v) solution to dye of 100. L micro 20 analysis ability impregnated gel MART of:1:2 mixture of DMEM (MART gel , BD Biosciences, Bedford, MA, American) to a low speed memory may barrier impregnated intermediate coated on the well chamber is formed on the resultant structure having performed for all the (intervening invasive barrier). Trans well cell migration and impregnated ability in assays, serum-derived cell migration activity as compared to the control group cells extract-treated the kill significant in a print head is reduced. Impregnated active (MART gel boundary is allowed to penetrate the ability) in cells-treated extract the kill also significantly reduced. 50-250 micro g/Ml the preparation of the extract the kill of the control group pending access requests in favor of a degree approximately 30-50% as compared to cells cell migration and impregnated to hold on to his temper-dependent capacity activity (also 2c, 2d also). < 실시예 6>Stationary fibronectincollagen (collagen) (fibronectin, FN) and the cellular response to-attaching capabilities analysis The cellular response to of extracellular matrix (ECM) sub-layer, adhesion of the tumor invasive at a steps by means of since the believe, in the present invention for-type collagen I fibronectin (FN) and in the worst condition, and adhesion of HT1080 the kill effect of extract were examined. 96-well culture plate extracellular matrix (extracellular matrix, ECM) the cellular response to attachment of analysis is performed for all the Taxus cuspidata. 5 micro g/Ml fibronectin (FN, Sigma) or 0.3% I-type collagen solution (Cell matrix type I-A; Nittazerachin Co. , Osaka, Japan) 50 micro l coated night at room temperature. Cold PBS to corresponding advertisement based on the shown list cleaning burn 2, 3% of bovine serum albumin (BSA) in 0.2 ml of DMEM with 1 37 °C in inconsistent with the server time was and washing it a (blocking). Cells suspended in serum-free DMEM (1 x 105 open/100 micro l) a frequency divides the ECM-coated culture plate after, 5% CO2 37 °C in incubator 1 in the incubation time. The non-attached for removing twice of cells attached and, after washing the cells at room temperature 0.2% crystal violet/20% methanol (w/v) solution to dye of 100. Dyeing cells are disclosed 0.2 ml of 1% sodium dodecyl sulfate (sodium dodecyl sulfate, SDS) solution been melting, spectrophotometer of measured at 560 nm (spectrophotometric) absorbency. HT1080 to destroy the cells that are the extract collagen and (FN) fibronectin when to preprocess for cell adhesion ability to the discharge circuit is reduced to a method-dependent the capacity (also 2e, 2f also). However, an extracellular matrix (ECM) the kill a well-coated to preprocess extract a well attached capabilities of cells did not (steps town do material). < 실시예 7>Blots analysis and active MMP-9 analysis blotting of HBV show that <7-1>Blots analysis and active MMP-9 analysis blotting of HBV show that Extracellular matrix (ECM) MMP-9 the ambient is decomposed to thereby tumor metastasis plays an integral role in since the are known as HMG-COa reductase, in the present invention extract the kill MMP-9 activity and regulates the expression whether wool yes blood and[...] blots were examined by graphitic gelatin. PMA increasing the expression activity and MMP-9 a strong active MMP-9 an introducer used as an. Time 12 cells serum-free DMEM in a certain concentrations of kill of 5 nm after incubation pre-extract the four balls myristate, and acetate (phorbol myristate acetate, PMA) 24 in further stimulates time. Gelatin graphitic wool yes blood MMT -9 activity through for irradiating the same set of the media conditioned with gelatin 0.1% capacity the n bit parallel data inputted electrophoretic in 8% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel), gels cleaning buffer (50 mm Tris-HCl, pH 7.5, 100 mm NaCl, 2.5% Triton X-100) and, after washing the twisting, twisting, to active buffer (50 mm Tris-HCl, pH 7.5, 150 mm NaCl, 10 mm CaCl2, 0.02% NaN3, 1 µm ZnCl2) is immersed after the incubation in 37 °C. Gels [...] brilliant blue (Coomassie Brilliant Blue) R-250 dyeing solution (Bio-Rad Laboratories, Hercules, CA, American) dyeing the n bit parallel data inputted, 10% isopropanol (isopropanol) (v/v) dealkylation in the dye of 100/10% acetic acid solution. MMP-9 of gelatin in the background blue dark resolution of low transparent 92 kDa was detected in size. Examining expression MMP-9 aqueous suspensions and to corresponding advertisement based on the shown list electrophoretic media with conditioned, electric (nitrocellulose membrane) with the cellulose just nitro performed for all the ferroelectric after transfer. Blots PowerOpti-ECL [...] detection reagent (Animal genetics, Inc. , Korean) and ImageQuant LAS 4000 mini (GE healthcare, Piscataway, NJ, American), a vinyl resin, and polyl visually of specific proteins using transition metal part directly visible, Image J software (American National hygienic circle) using the band strength the bill. Also as main viewed at 3a and 3b, the kill of 50-250 micro g/Ml PMA processing extract MMP-9 pads by increased expression of activity and a capacity-dependent method in dramatically reduced. Idle the inhibitory effect of the was that may have been be. <7-2>Kill the extract induced by PMA κB-NF breaker which cuts off the phosphorylation of ERK activity and Transcription factor NF-κB different MMP-9 by PMA in cells wherein the expression induction of tumor invasive and sections are alternately arranged from a tracked used centrally and which are involved in the been the step of etching a metal film. MMP-9 expression and impregnated inhibitors of extract the kill capacity NF-κB inhibition activity of a circuit is connected or is connected for irradiating an whether an, corepressors (nuclear translocation) with noise amplifier, a phase shifter, in the present invention p65 protein IκB and subjected to the blots were examined in [...] level of IκB. M-PER mammalian protein extract reagent (M-PER mammalian protein extraction reagent) and NE-PER nuclear/cytosolic extraction reagent (Pierce biotechnology, Rockford, IL, American) using whole cells suspension and nucleus, thus/cytosolic extract (cytosolic, cytoplasm of liquidus portion) shown. Also as main viewed at 4a, the control group HT1080 be stimulated by a stimulus PMA in IκB phosphorylation and while increased immediately is IκBα, HT1080-treated extract the kill the control group cells in significantly low as compared to cells. In the control group, after stimulating PMA protein p65 the nuclear cytosolic was positioned in to quickly conveying. In cells-treated extract the kill, mobile position in nuclear p65 is suppressed was significant (also 4b). These observations are ISDN to, restraining active NFκB extract the kill reduce the activity of the MMP-9 HT1080 by infiltration and additional amino acid to n-terminal. suggesting a that inhibitors. Many and in research, ERK1/2, p38, including MAPKs JNK1/2 a coordinator site above AP-1 or NFκB is acting as an active MMP-9 it is involved in and expression has been reported. As on the 5 also, in the control group through stimulation PMA p38, ERK 1/2, phosphorylation of with the understanding that it will not induced JNK1/2. While kill a cells in the extract and pads by PMA is suppressed been significant is phosphorylation of ERK1/2, p38 JNK1/2 and there was hardly any effect can be for phosphoric acid. The kill PMA extract the pads by inhibiting ERK activation is MMP-9 inhibition activity of. suggesting a that is associated with. < 실시예 8>Measuring of intracellular ROS Previous experiments various in PMA is ROS production through NFκB activity through active MMP-9 and that trigger, ROS production expression of activity MMP-9 shows that contribute to. The shelf life of food by inhibiting by the kill MMP-9 relates to ROS-removing activity to determine, in the present invention (flow cytometer) analyzer cytometry using PMA in HT1080 pads by a ROS decided effect of extract the kill. Hydrogen peroxide-susceptible warm-fluorescence probes '7' -dichlorofluorescein diacetate (peroxide-sensitive fluorescent probe) 2 (DCF-DA, Sigma) in cell by using ROS levels was assessed. Kill the extract or (N-acetyl-L-cysteine) N-acetyl-L-Γ-butyrolactone cell has a specified time after incubation pre [...] or pre without corresponding advertisement based on the shown list as PMA of 5 nm, then 30 minutes in 37 °C DCF-DA (5 µm) incubating the sample with the. Cells washed with PBS, intracellular ROS levels CellQuest software (BD Biosciences, San Jose, CA, American) FACSCalibur using cytometry analyzer (flow cytometer) to the n bit parallel data inputted immediately measuring, WinMDI 2.8 software (J. Trotter, Scripps Research Institute, La Jolla, CA, American) was subject to analysis by. Prior to as reporting, PMA irritation even the present invention (-6.5-back) intracellular ROS level modification markedly increases the ROS by pre-treating pre NAC and that almost perfect it has been confirmed that can be readily removed (also 6a). Furthermore, the NAC IκBα derived stimulus PMA IκBα decomposition completely reduced in the content of phosphoric acid and corresponding advertisement based on the shown list, -adsorbers reduces the expression of activity and MMP-9 (also 6b-c), the HT1080 pads by PMA in ROS production activity and κB-NF associated increase activity MMP-9 by a rope. suggesting or not. Advantageously interesting, also extract the kill PMA stimulus as compared to the control group cells, in an extended intracellular ROS production to synchronize the reduced by -70%, IκBα phosphorylated IκBα and transmits the decomposition, was for inhibiting the activity of MMP-9 (also 6a-c). Such material through extract the kill κB-NF ROS signaling by inhibiting the activation PMA stimulus derived activity suggesting a that is inhibited from increasing.. < 실시예 9>Empirically in vivo waste transition analysis In the present invention C57BL/6J intravenous tail mouse B16F10 that is poured in a transition to the lung cells is that proliferate inhibitors of extract the kill capacity were examined effects. B16F 10 cells (3 x 105 one cell/0.2 ml PBS) a female mouse tail veins C57BL/6J he infused a through (0). Mouse 3 optionally a massage cream, an essence, differently asked (n=5 each each group), the kill simultaneously with induction of transition daily extract was useful when administered orally the kill of. 50 or 100 mg/kg/day carrier extract (as saline) was administered for a into MICE and a mouth, and to 17. Has been sacrificial mouse, mouse lungs voting a solution (Bouin ' s solution) the n bit parallel data inputted fixed (Sigma), waste on surfaces of B16F10 number of colony color of the composition, of a gum cells for visual inspection decided to get out of. Also as also on the 7a to 7c, the control group from the group administering extract the kill of light emitted from at least the and significant colony is metastatic waste black a print head is reduced (each 50 and 100 mg/kg signals from the and the control group value corresponding to -15%-20%). Iteratively extract the kill period experiment administration of a non-toxic systemically mouse for irradiating an whether, as saline the composition in an equal amount and (the control group) or 50 or 100 mg/kg concentration of kill the extract was the daily administration of orally. When by administering the test extract the kill during 15 or abnormally viscous or cohesive death without causing implying behavioral, mouse of weight gain of (table 1) for increasing efficiency of client-server or each weight (table 2) no influence on. Serum therapeutically in assays, the ratio BUN/CRE GOT/GPT and extract the kill the significant group out-administration have not been changing, the kill the extract route of administration is of liver injury or renal damage was, does not induce enhanced. suggesting (table 3). Hematological in assays, hemoglobin numeral and of red blood cells and the level (indications of anemia) by the kill the significant un-varying in the, treated extract the kill and number of leukocyte from mouse normal all other parameter was to in the range (table 4). The kill such material without adverse effect in vivo when by administering the test extract B16F10 transition effectively suppressed by waste of cells show steps, detailed hereinabove. Average ± have been shown by the standard deviation of the above material. (N=3) the respective group of mouse 50 or 100 mg/kg daily for the corresponding advertisement based on the shown list oral administration, 0, 5, 10, it was determined that weight to 15. Average ± have been shown by the standard deviation of the above material. (N=3) the respective group of mouse 50 or 100 mg/kg daily for the corresponding advertisement based on the shown list oral administration, then the sacrificial 15 it was determined that weight for increasing efficiency of client-server. Average ± have been shown by the standard deviation of the above material. (N=3) the respective group of mouse 50 or 100 mg/kg daily for the corresponding advertisement based on the shown list oral administration, to synchronize the sacrificial 15, GOT, GPT, LDH (lactose dehydrogenase), BUN (blood urea nitrogen), and analyzing the CRE (creatine). Average ± have been shown by the standard deviation of the above material. (N=3) the respective group of mouse 50 or 100 mg/kg daily for the corresponding advertisement based on the shown list oral administration, to synchronize the sacrificial 15, analyzing the hematological parameters (parameters). Said description of abbreviation of a table as follows. CBC, former blood corpuscle inspection (complete blood cell count); WBCP, leukocyte blood cells inspection haloperoxidase method (white blood cell count peroxidase method); WBCB, leukocyte blood cells inspection good salt nine method (white blood cell count basophil method); RBC, red blood inspection (red blood cell count); HGB, hemoglobin (hemoglobin); HCT, hematocrit (hematocrit increase that, hematocrit); MCV, (mean corpuscular volume) average hematocrit increase that; MCH, average red blood hemoglobin (mean corpuscular hemoglobin); MCHC, average red blood hemoglobin concentration (mean corpuscular hemoglobin concentration); PLT, platelet (platelet); NEUT, neutrophil (neutrophil); LYM, lymphocytes (lymphocyte); MONO, monocytes (monocyte). Including useful as anti-the kill the present invention refers to, metastasis a pharmaceutical composition and is provided to inhibit metastasis is directed to a food composition for inhibiting.. The kill of the present invention including extract composition properties, and hardly side effects a low transition matrices have plural polypropylene matrix fabric advantages can be, transfer or transition related disorders therapeutic agents, or transfer or transition-associated diseases, preventing or ameliorating formulations, as well as a functional food which may be can be useful. Kill the hot-water extract as an active ingredient for inhibiting tumor metastasis including pharmaceutical compositions. Deleted According to Claim 1, 0.001 weight % to 50 weight % of a hot-water extract the kill a including pharmaceutical compositions for inhibiting tumor metastasis. According to Claim 1, pharmaceutically acceptable carrier, excipient or diluents number further including pharmaceutical compositions for inhibiting tumor metastasis. Kill the hot-water extract as an active ingredient for inhibiting tumor metastasis including food composition. Deleted According to Claim 5, 0.001 weight % to 50 weight % of a hot-water extract the kill including a food composition for inhibiting tumor metastasis. Deleted Deleted Processing Weight (g) 0 5 10 15 The control group 17.43 ± 0.36 18.68 ± 0.57 18.82 ± 0.73 19.21 ± 0.41 50 mg/kg 17.38 ± 0.34 18.51 ± 0.42 18.64 ± 0.30 19.03 ± 0.52 100 mg/kg 17.34 ± 0.35 18.65 ± 0.45 18.99 ± 0.12 19.34 ± 0.17 Processing (G) weight for increasing efficiency of client-server liver Heart waste Operation errors of the device Elongated (left) Elongated (right) The control group 1.08 ± 0.04 0.10 ± 0.02 0.15 ± 0.01 0.08 ± 0.01 0.12 ± 0.01 0.12 ± 0.01 50 mg/kg 1.08 ± 0.01 0.10 ± 0.01 0.13 ± 0.01 0.07 ± 0.00 0.11 ± 0.00 0.12 ± 0.01 100 mg/kg 0.94 ± 0.08 0.11 ± 0.01 0.15 ± 0.01 0.08 ± 0.01 0.12 ± 0.01 0.12 ± 0.02 Processing GOT (IU/L) GPT (IU/L) LDH (IU/L) BUN (IU/L) CRE (IU/L) The control group 52.7 ± 3.1 29.3 ± 4.6 518.7 ± 78.0 36.5 ± 1.5 0.7 ± 0.1 50 mg/kg 56.0 ± 6.3 28.0 ± 1.4 424.0 ± 83.7 36.8 ± 3.6 0.4 ± 0.1 100 mg/kg 59.0 ± 2.0 30.0 ± 2.0 576.3 ± 72.3 39.0 ± 5.2 0.8 ± 0.3 (Parameter) parameters The control group 50 mg/kg 100 mg/kg WBCP (x103 l/micro cells) 1.8 ± 0.58 1.9 ± 0.27 1.9 ± 1.03 WBCB (x103 l/micro cells) 1.9 ± 0.56 1.8 ± 0.21 1.9 ± 1.01 RBC (x106 l/micro cells) 9.5 ± 0.30 10.4 ± 0.61 9.4 ± 0.01 HGB (g/dL) average 13.6 ± 0.30 13.9 ± 0.06 13.7 ± 0.00 HCT (%) 54.5 ± 1.01 53.3 ± 1.11 53.8 ± 0.64 MCV (fL) 57.5 ± 1.10 59.6 ± 0.54 57.5 ± 0.63 MCH (pg) 14.4 ± 0.26 14.3 ± 0.03 14.6 ± 0.01 MCHC (g/dL) 24.9 ± 0.57 24.0 ± 0.51 25.5 ± 0.28 PLT (x104 l/micro cells) 97.1 ± 17.5 95.8 ± 11.4 89.3 ± 1.84 % NEUT 9.7 ± 1.96 9.20 ± 0.07 9.9 ± 0.37 % LYM 86.5 ± 1.85 90.5 ± 0.25 85.7 ± 2.62 % MONO 0.5 ± 0.11 0.5 ± 0.09 0.5 ± 0.10
