FOOD COMPOSITION CONTAINING PRICKLY ASH SEED OIL FOR PREVENTING OR TREATING ARTERIOSCLEROSIS
The present invention refers to food compositions containing the alternative space, a heating medium oil recovery of weakening relates to a, more particularly atherosclerosis containing alternative space, a heating medium oil recovery of weakening for treatment or prevention. is directed to a food composition. Recent franchises group motion part and applies an a roasted nutty taste and onset of adult due to the is, developing artery at the population for veteran. there is a trend on the increase. Arteriosclerosis various quality life of patient with complications causes problems improve since, the 'hljsd' is treatment and management diseases.. Vascular health of a and for vascular for modulating blood pressure order keep foreign materials from blocking the deposition of. most important. Together with pulsation surface of the heart the blood pressure, vascular endothelial cells and muscle contraction and relaxation of cells is held while the organic are adjusted steplessly by means. Pressure vessel as blood flow or is calculated if both blood vessels are appropriately relaxation upon a failure, blood pressure is a such that the gaseous support medium rises, thereby hypertension, arrhythmia, heart failure, which has a weight corresponding to weight coin artery. Representative angiogenesis relaxation factor include vascular endothelial cells endothelial nitric oxide synthase (endothelial nitric oxide synthase, eNOS) generated by a nitric oxide (nitric oxide, NO) easily is known, the anti atherosclerotic curing (anti-atherogenic) effect are induced to is also known as. A nitric oxide produced in endothelial cells vascular smooth muscle thereby to block the movement of the intracellular acid enzyme neel it buys the ring (guanylate cyclase, GC) and engage the, a activated neel buys the ring enzyme guanosine triphosphate (guanosine triphosphate, GTP) for of cyclic guanosine monophosphate (cyclic guanosine monophosphate, cGMP) by, is configured to activate 'cGMP-dependent protein kinase G (PKG)', 'Ca2+-activated K+' channel ' or myosin light chain phosphoric acid which increase the activity of protease (myosin light-chain phosphatase), through the blood vessel to induce relaxation is especially. Vascular health another method for maintaining vascular foreign materials from accumulation of the recording operation. film. In particular, (atheroma) creation and induction intravascular gruel kind that affect various adhesion molecule (cell adhesion molecule, CAM) VCAM-1 (vascular cell adhesion molecule-1) and ICAM-1 (intercellular adhesion molecule-1) during the for inhibiting the expression of the for maintaining vascular health is an important element. While, human angiogenic endothelial cells (human umbilical vein endothelial cell, HUVEC) tumor in necrosis factor-alpha VCAM-1 and ICAM-1 (TNF-α) data unit incremented by the for inhibiting the expression of atherosclerosis may assist in to improvements in it has been reported that. Proliferation of HUVEC TNF-α data unit incremented by angiogenesis (proliferation) cured by influencing and can help growth of cancer cells, such as psoriasis disease due to angiogenesis since are capable of inducing, which decreases same to angiogenesis with anticancer it can be raised by protective boards diseases prevention of. since. Medicament for treating arteriosclerosis heaps further developed in the so-but, existing pharmaceuticals and containing an edible raw side effects are, some drug severe causing adverse effects and the years ago, he used to imitate and unrestricted use. Therefore, side effects using natural products it is for the treatment or prevention of arteriosclerosis be carried out at a racing where many an effort. The present invention refers to using alternative space, a heating medium oil recovery of weakening as natural not fall dead in spite of adverse excellent midia, capable of having a. provided composition. Alternative space, a heating medium oil recovery of weakening the present invention refers to as active ingredient arteriosclerosis characterized by containing a provides a food composition for treatment or prevention. In the present invention 'composition' a meaning of the present invention of food composition cleaned is pumped to preventing or ameliorating arteriosclerosis oil fruit recovery of weakening of the present invention generated from meaning that it, other component, in one example food stabilizer, auxiliary number, number formulations such as the torsion bar is used for is to be added.. Furthermore, preventing or ameliorating arteriosclerosis good effect are also other ingredients means a may be added. The present invention refers to specific nutraceutical formulations which are not limited to, beverage added to of publicly known and may be formulated in drinks, and a method for manufacturing the same publicly known finished pieces nutraceutical formulations may be added as auxiliary. Recovery of weakening the inflammatory ( In the present invention alternative space, a heating medium oil recovery of weakening person using useful in inhibiting and treating the vascular endothelial cells (human umbilical vein endothelial cell), vascular relaxation the enzyme endothelial nitric oxide synthase (endothelial nitric oxide synthase) a attachment factors and vessels, wherein the be achieved, wherein an influence on vascular cell adhesion molecule (VCAM) - 1 and intercellular adhesion molecule (ICAM) - 1 have been confirming be achieved, wherein an influence on, experiments, arteriosclerosis is the present invention excellent effects of preventing or ameliorating exert can be confirm that the user. While, in the present invention, recovery of weakening said recovery of weakening preferably Δ3 fruit fat-soluble solvent extraction loss is obtained by single and of using a, fat-soluble solvent in one example of cyclohexane to, use can be made of,. While, in the present invention, recovery of weakening said Δ3 fruit, fat-soluble loss is recovery of weakening vehicle speed is lower than the extracted ultrasonic by the application of solvent (a); said after extraction, (b) consists in impregnating; and said wt, filtration and concentrating (c); a process including obtained therefrom of using it is still more preferably. Hereinafter, said step by each stage. described pulsating current is. < 단계 (a): 초음파 추출 > Present recovery of weakening step loss is fat-soluble solvent is added to the ultrasonic vehicle speed is lower than the extracted. Present fat-soluble solvent extraction one step by involve the use of solvents and fruit ( bell room ) to recovery of weakening is a fat-soluble material, i.e. oil ( [...] ) ingredients in a brewing can be. Fat-soluble solvent in one example, of cyclohexane to which can be used, preferably by a factor of recovery of weakening 2-5 contrast weight of recovery of weakening is added loss is preferably. On the other hand, preferably fruit recovery of weakening one step present carbonate and efficiency enhanced and the are used in rich in functional ingredients, recovery of weakening, preferably the degree of grinding degree 15-25 mesh (mesh). On the other hand, present to give supersonic waves step which ultrasonic extraction, ultrasound having an intensity 50-70 Hz and of using, 15-30 time preferably extracting minutes. < 단계 (b): 침지 > Present said after extraction step, is consists in impregnating. Immersion for extract of excited after passage of ultrasonic waves through the has the size sufficient a contact method by being mounted, in fruit recovery of weakening is a fat-soluble components to be the release of an. The, preferably at a temperature 3-7 °C the needle it falls 12-36 preferably it performed during time. < 단계 (c): 여과 및 농축 > Wt said step present, filtration and concentrating is. Through filtering by removing animal can be obtained pre-cleaned, extract. The paper filter (filter paper) or glass fiber filter (glass fiber filter) can be used for a, preferably every first using paper filter and, then, glass fiber filter through a preferably a filtration once more in. While, filtered extract is concentrated and if there is the specific hydrophobic penetration hole while moving up and down. 40-45 °C under temperature of the pressure-reducing concentrate is preferably. The, step of minimize destruction of. since. Because the derived natural composition of the present invention without adverse side effects, excellent experiments of the present invention and exhibiting efficacy midia sequences as signal. Figure 1 the present invention show production process oil fruit recovery of weakening. Cytotoxicity on HUVECs Figure 2 the present invention valuable fruit recovery of weakening more, exhibiting result. Figure 3 the present invention in generating valuable fruit recovery of weakening eNOS be achieved, wherein an influence on HUVECs show. HUVECs in Figure 4 the present invention valuable fruit recovery of weakening be achieved, wherein an influence on protein expression ICAM-1/VCAM-1 show. Figure 5 the present invention, a battery supplies a fat type between alternative space, a heating medium oil recovery of weakening SD-rat blood cholesterol (cholesterol) show variation. Recovery of weakening the present invention between type fat Figure 6, a battery supplies a alternative space, a heating medium oil (triglyceride) blood triglyceride of SD-rat show variation. Figure 7 alternative space, a heating medium oil recovery of weakening the present invention, a battery supplies a fat type between blood SD-rat show variation (cholesterol) cholesterol-HDL. Alternative space, a heating medium oil recovery of weakening the present invention Figure 8, a battery supplies a fat type between blood SD-rat show variation (cholesterol) cholesterol-LDL. Figure 9 the present invention, a battery supplies a fat type between alternative space, a heating medium oil recovery of weakening SD-rat blood arteriosclerosis index show variation (atherogenic index). Hereinafter, embodiment of the present invention content to to pulsating current is. described. Just, to range rights of the present invention are not limited and only in the embodiment, equivalent thereof includes collect the blood and to easily deformation of the idea. [In the embodiment 1: manufacturing oil fruit recovery of weakening of the present invention] Recovery of weakening rich in functional ingredients, carbonate and to 20 mesh (mesh), for taking the results of a 500 g, added a (n-hexane) 1.4 L hexane normal herein. After, to give supersonic waves the extracts the 30 minutes, at a temperature of 5 °C visitor is checked through a immersion for time 24. Wt, using only watt No.2 filter paper (Whatman No.2 filter paper) after performing a filtering difference 1, 'Advantec GF-75' glass fiber filter (Glass fiber filter) using filtering performed for all the 2 difference. 2 after filtration difference, to a concentration is reduced in pressure by the 45 °C use at been produced with at they finally result sample oil fruit recovery of weakening (also reference 1). [Experiment e.g. 1: using HUVEC midia efficacy verification] 1) HUVEC culture A favorite gangwon vascular study at high speed to enable the human cord blood derived vascular endothelial cells the DMEM (Human umbilical vein endothelial cell, HUVEC) ('s modified eagle' dulbecco s medium) to 1% and 10% serum (fetal bovine serum) [...] by adding (Penicillin and streptomycin) antibiotic 37 °C, 5% CO2 good after to adapt the incubator (incubator). Cells sufficient to obtain the n bit parallel data inputted for cultivating cells in said multi function cap T75, T75 multi function cap bottom 60% redundancy repair and apparatus for sensing particles on stage antitrypsin-surface cultured cells is released (trypsin-ethylene diamine tetraacetic acid, trypsin-EDTA) solution (1x) using attached with the aid of cells stripped off after, sebocytes are excellently the embodiment. 2) measuring toxicity on HUVECs significant fruit recovery of weakening (1) experiment method Culture is completed cells to 96 well tissue culture plate (tissue culture plate) 1x104 cells/ml to 100 μ L at time 24 and the CO2 incubator in medium process has been completed. 24 good alternative space, a heating medium oil recovery of weakening by etching are removed and then behind senses a rotation velocity of the disk to DMSO concentration by dilution to of medium for cells to culture nor post-incubation time good time 24 and. 24,10 μ L solution reagent CCK-8 the culture medium to which under culture applies and, again in 37 °C 3 hours' extra by culturing tetra thiazolium salt (tetrazolium salt) capable of reduction and depollution of formazan predetermined 450 nm for (formazan) measuring absorbance in cells was verifying the survival rates. (2) experiments As present in Figure 2, oil fruit recovery of weakening 0.01-0.1 mg/ml concentration of 100% initializes purchases the passenger in the adaptation, ion conductor of 0.2 mg/ml have been shown to about 80% degree of cell survival rate. 3) recovery of weakening measuring secretion nitric oxide significant fruit (1) experiment method Grease amount secretion nitric oxide significant fruit recovery of weakening (Griess Reagent), identified through use of a the reagent. 96 well tissue culture plate for HUVEC 1×105 cells/well to 100 μ L at 24 and the after grown on time, FBS DMSO to alternative space, a heating medium oil recovery of weakening behind senses a rotation velocity of the disk without added is in a medium to TNF-α and dilution by concentration (100 ng/ml) after treatment into a cell, good 24 . 24 process has been completed in 37 °C time, 100 μ L medium under culture (Griess) grease the composition in an equal amount and to extended storage and is easily carried reagent reacting a metal salt of minutes 10 ELISA microplate reader (microplate reader; EL808 ; BioTek, Winooski, USA) it was determined that absorbance in 540 nm using. (2) experiments As present in Figure 3, oil fruit recovery of weakening during processing TNF-α concentrations greater than 0.05 mg/ml (Nitric oxide synthase, NOS) nitric oxide synthase in the activity of the 100% initializes purchases the passenger the control group contrast, 0.01 mg/ml of 90% shown to contrast the control group ion conductor. 4) significant fruit recovery of weakening ICAM-1/VCAM-1 be achieved, wherein an influence on protein expression (1) experiment method , Intracellular protein extract: alternative space, a heating medium oil recovery of weakening to HUVEC at a concentration of 0.1 mg/ml then processing time 24 after removing the medium, in a manner which permits the scraper cell (cell scraper) having a bottom (well) well to both cells 1.5 ml into a tube the supernatant may be processed to recover (protein extraction solution) protein extract solution after applying a 1-20 °C carried [...] strongly minutes 20 minutes in the storage. 12,000 g solution, 5 minutes in 4 °C by using a liquid upper layer centrifugal separator, the third to eo experiments. , Recording disc surfaces: 595 μ L distilled water to 5 μ L protein extracted after dilution agent is placed [...] distilled and a 200 μ L reagent (bradford) pod 200 μ L into the 5 seconds after 5 minutes after mixing the ingredients in is diced [...] reaction then it was determined that absorbance 595 nm in. Standard material the to quantitatively using a solution of BSA. , Membrane dotting (Membrane dotting): 6x the instant proteins and are separated off and the loading die (loading dye) 95 °C behind in the weight ratio of 5:1 for 6 minutes in boiling device is shown. Membrane protein-prepared sample 3 μg/2 μ l at predetermined intervals on the (dotting) dotting at 1 at room temperature was very dry time. , Blocking (Blocking) and antibody response (antibody reaction): membrane protein antibody from other parts except for a gate and does not react to 2 to 5% milk scheme (skim milk solution) solution after treatment time using buffer TBS-T membrane and, after washing the, 1 difference antibodies that are responsive to target protein (primary antibody, 1% BSA solution) and the processor during time 2 a, 2 difference antibodies (secondary antibody, 5% skim milk solution) to 40 minutes after treatment TBS-T the washed by means of buffer. , Chemiluminescence detection (chemilluminescence detection): membrane on antibody binding time solution detecting blots membrane (western detection solution, substrate, 1 ml each enhancer) in darkroom after applying the bands radiating light onto a liquid membrane protein expression confirms pt service identities, respectively then the same thickness as that of the Image J strength of band thereof (National institutes of health, USA) the n bit parallel data inputted is quantitatively analyzed by use software, GAPDH as the first voice portion out of an internal standard. (2) experiments As present in Figure 4, when various alternative space, a heating medium oil recovery of weakening, ICAM-1 protein and VCAM-1, where the expression of the protein was capable of verifying is opened. [Experiment e.g. 2: RAT a midia efficacy verification] 1) preparing model animal Present recovery of weakening in the experiments using SD-rat verifies the efficacy midia significant fruit animal experiments is performed for all the. The contents address memory, using 'sprague dawley rat (SD rat)' 20±5°C week zero 10, humidity 55-60%, contrast 12hr between type standard regulation unit moves simultaneously at the same period the water level for sufficiently wetting the laundry 1 weeks the breeding adaptation time environment. For SD rat completed adaptation each using a collection experiment the difficulty lump law corresponding advertisement based on the shown list classification, general formula of this group (normal), fat/elevated cholesterol group (HFHC), simvastatin administration (HFHC+simvastatin) group, of cyclohexane to extracted using a recovery of weakening 7,15% group measured under the different kind of dietary elevated cholesterol/fat alternative space, a heating medium oil then anhydrous by an epoxy molding material having a group of administration supply administering dietary the dietary elevated cholesterol/fat daytime. 4 the administering obesity/arterial dehydrating the resulting corresponding advertisement based on the shown list, simvastatin (simvastatin) recovery of weakening are mixed together intake dietary vegetable fat and significant fruit midia efficacy has been confirmed. 2) biochemical analysis of an lipid blood Blood lipid biochemical analysis of an SD-rat blood for blood tail from 3,000 rpm 15 minutes after as blocks, in centrifugal separator are obtained the serum total cholesterol (total cholesterol, TC), neutral lipid-depleted (triglyceride, TG), high density lipoprotein cholesterol (high density lipoprotein cholesterol, HDL-C) content of it was determined that as follows. 3) total for measuring the cholesterol Set sub measurements total cholesterol in blood plasma of hour misfortune for measuring total cholesterol (AM 202-K, ASAN, Korea) was by using. Enzyme reagent dilution to buffer solution then with serum ampule hour misfortune the enzyme to each (cholestrol 300 mg/dL) 0.02 ml 3 ml for inserting and removing the mixed and are 5 minutes in 37 °C pattern critical dimension MTT and CDU then reaction using (spectrophotometer), thereby measuring absorbance in 500 nm as well as the calculated. [Expressions 1] Experiments, as in Figure 5 present alternative space, a heating medium oil recovery of weakening when dietary added, total cholesterol content of was capable of verifying is opened. 4) in blood plasma of neutral fat quantity measuring In blood plasma of neutral fat quantity hour misfortune for measuring total cholesterol set sub measurements (AM 157S-K, ASAN, Korea) was by using. Enzyme reagent dilution to buffer solution then with serum ampule hour misfortune the enzyme to 0.02 ml each (triglyceride 300 mg/dL) doesn't have any error frames, the mixed and are 3 ml 10 minutes in 37 °C pattern critical dimension MTT and CDU then reaction using (spectrophotometer), thereby measuring absorbance in 550 nm as well as the calculated. [Expressions 2] Experiments, as present in Figure 6, when dietary added alternative space, a heating medium oil recovery of weakening content of neutral lipid-depleted can be provided to. 5) HDL cholesterol-measuring Set sub measurements cholesterol-HDL in blood plasma of 'HDL-Cholestase (AM 203, ASAN, Korea)' was by using. Serum 0.2 ml 0.2 ml reagent separate for inserting and removing the mixed and are 10 minutes at room temperature after incubation the, 3000 rpm in 10 minutes by cylinder to have the centrifugation supernatant. Enzyme reagent dilution to buffer solution then with serum ampule (dL/cholestrol 50 mg) each hour misfortune the enzyme to 0.1 ml 3 ml for inserting and removing the mixed and are 5 minutes in 37 °C pattern critical dimension MTT and CDU then reaction using (spectrophotometer), thereby measuring absorbance in 500 nm as well as the calculated. [Expressions 3] Experiments, as present in Figure 7, when dietary added alternative space, a heating medium oil recovery of weakening HDL-cholesterol content increases can be confirm that the user. 6) LDL cholesterol-measuring [...] pre content in blood plasma of cholesterol-LDL (Friedewald) method (Clinical Chemistry June 1972 vol. 18 no. 6 499-502) using calculation of the bill as follows. [Expressions 4] Experiments, as present in Figure 8 added alternative space, a heating medium oil recovery of weakening when dietary cholesterol-LDL content was capable of verifying is opened. 7) arteriosclerosis index calculated Artery curing surface or both surfaces of the Publication measure a risk for atherosclerosis (Atherogenic index) index following the next calculation of the calculated using method. [Expressions 5] On which the calculated results, as in Figure 9 present added alternative space, a heating medium oil recovery of weakening when dietary shown low index arteriosclerosis can be confirm that the user. The present invention relates to a food composition containing prickly ash seed oil for preventing or treating arteriosclerosis. The food composition is derived from a natural substance, and therefore has no side effects, and has an excellent anti-arteriosclerosis effect. COPYRIGHT KIPO 2016 Fat-soluble solvent loss is recovery of weakening of cyclohexane to applied vehicle speed is lower than the extracted ultrasonic minutes 30 (a); said after extraction, consists in impregnating time in 5 °C 24 (b); and said wt, filtration and concentrated and the recovery of weakening (c) obtain alternative space, a heating medium oil; includes, Δ3 fruit recovery of weakening said endothelial nitric oxide synthase (endothelial nitric oxide synthase) and increase the activity of, emulating cell-to-cell junction molecules 1 (intercellular adhesion molecule -1 (ICAM)) and vascular cell junction molecules expression of 1 (vascular cell adhesion molecule -1 (VCAM)) relates to an arteriosclerosis characterized by manufacturing method of food composition for treatment or prevention. Deleted Deleted Deleted According to Claim 1, the hexane said, recovery of weakening by a factor of 2-5 contrast weight of recovery of weakening loss is for the addition of an arteriosclerosis characterized by manufacturing method of food composition for treatment or prevention. Deleted General formula of this group Group the user to practice their elevated cholesterol fat Fat (simvastatin) simvastatin group the user to practice their elevated cholesterol Group the user to practice their elevated cholesterol fat vegetable fat intake group ↓ ↓ ↓ ↓ Normal HFHC HFHC+simvastatin 0.02g/kg diet 7,15% oil fruit recovery of weakening HFHC+ Normal HFHC Simvastatin (Simvastatin) HFHC+ HFHC +oil 7% HFHC + oil 15% Casein (Casein) 200 200 200 200 200 Maize starch (Corn Starch) 223 223 223 223 223 Sucrose (Sucrose) 250 250 250 250 250 Cellulose (Cellulose) 50 50 50 50 50 Vegetable fat (Vegetable oil) 70 150 (Soybean Oil) crankshaft 70 150 150 80 (Lard) strength copper alloy 70 70 70 70 Mixing mineral (Mineral Mix) 35 48 48 48 48 Mixing (Vitamin Mix) vitamin 10 21 21 21 21 Cholesterol (Cholesterol) 12.5 12.5 12.5 12.5 Bile (Cholic acid) 5 5 5 5 Drug 0.02
