HANGOVER-RELIEVING COMPOSITION COMPRISING ALLIUM HOOKERI AS ACTIVE INGREDIENTS

Hereinafter, the present invention is described therein that are directionally.

The present invention refers to three while (Allium hookeri) As an active ingredient a hangover curing composition number under public affairs substrate.

The number bath including manufacturing method to process the extract while said separation preferably are but not limited to:

1) extracting the extraction solvent while three applied;

2) step 1) extract of filtering step;

3) step 2) drying the extract cream extract after filtering of step number three while tank.

In said method, step 1) while the cultivation of the bolt or a commercially available can be used without a like number, leaf, root, stem and is enabled to any center, while not limited to the use of the present invention preferred embodiment of plant leaf or leaves but most preferably is of the triple-eastern inside muscle connection.

In said method, step 1) extraction of the solvent can be water, alcohol, organic solvent or a mixture of these and the main preferably. Said alcohol include C₁ To C₂ Use is preferably lower alcohols, lower alcohols include ethanol or methanol preferably using. It freely extraction extraction method, using Soxhlet extraction or reflux extraction preferably but not limited. Said leaf or root powder dose 1 to 30 times while three extraction solvent preferably added to extraction, extraction 20 times more preferably added. Preferably 20 °C to 100 °C in extracting temperature, more preferably in 20 °C to 40 °C, but is most preferably an room temperature, not limited. In addition, extraction time is 10 to 48 is preferably a few times, and more preferably in a time 15 to 30, most preferably an 24 time but, not limited. In addition, extraction is preferably the number 1 to 5 it is a sliced raw fish, more preferably 2 to 4 times extracting, or most preferably it is a sliced raw fish 2, limited to are not correct.

While 95% and preferably 40 to extract said three main extract, more preferably 50 to 90% is a main extract, a main 60 to 80% and most preferably an extract, said 40% hereinafter extraction efficiency is lowered in the case of a main extract target hangover effect and may be, 80% main extract, while the triple-extract cost of break manually his fellow workers.

(Alcohol dehydrogenase; ADH) and aldehyde dehydrogenase while three said extract has dehydrogenase (aldehyde dehydrogenase; ALDH) of characterized in that interaction.

In the embodiment of the present invention in particular, the present invention while the victims of the number of leaf and root portion 70% using fermentation extract have three main high pressure liquid coolant, the same HepG2 hepatocyte cell lines when it has processed, while three said alcohol dehydrogenase and aldehyde dehydrogenase activity extract significantly active at the time of identifying (also reference 1), while three of the present invention can be useful for removing hangover extract is present in the composition.

In addition to the extract of the present invention composition for hangover while three same or similar functions can be containing at least one active principle 1.

Oral or parenteral administration of the present invention composition for hangover clinical material which upon administration, can be used in form of a general pharmaceuticals number number. I.e., a production method of the present invention hangover oral and parenteral composition for chamber number can be administered clinical administration number type, there is a guaranteed number number number under anger normally used, weight number, number coupled, wet number, such as disintegrating dilution number or excipients using tank number number number surfactants and number are disclosed. Positive number number number is solid for oral administration, ring number, acid number, number and capsule number granules and the like, of the present invention composition comprises at least one excipient such solid number number number for example, starch, calcium carbonate, sucrose, lactose and gelatin are number tank pressure is lowered. In addition, simple number excipients in addition to magnesium, [su[su] mote [ley[ley] [thu[thu], lubricating number such as the center of gravity of talc are used. For oral administration include liquid suspension number number number, content number, which number corresponding wire number and syrup or the like, commonly used in a simple water dilution number, in addition to the paraffin droplet number various excipients, for example wet number, sweetener number, direction number can be like number and preservation. For parental administration number number is a sterile aqueous solution, non-aqueous for number, number suspension, oil number, group number number number left freeze and multiple myelomas are included. Number include propylene glycol for suspension on non-aqueous for number, polyethylene glycol, vegetable oil such as olive oil, an injectable the [ley[ley] which comes the [thu[thu] and ethyl ester is used as the alkali such as can be. (Witepsol) include above [theyp[theyp] brush left number for number, mark with goal, twin (tween) 61, carcass five fingers, the it soaked, can be glycerol and gelatin is used as the alkali. Composition for parenteral administration of the present invention hangover hypodermic injection, intravenous or intramuscular scanning be brushes.

Of the present invention hangover composition for the preferred dosage is patient state and body weight, degree of disease, forms, according to the stated time routes of different but, can be appropriately selected by one skilled.

In addition, while the present invention refers to separation (Allium hookeri) As an active ingredient non-food hangover number under public affairs substrate.

The number bath including manufacturing method to process the extract while three said preferably but not limited to:

1) extracting the extraction solvent while three applied;

2) step 1) extract of filtering step;

3) step 2) drying the extract cream extract after filtering of step number three while tank.

In said method, step 1) while the cultivation of the bolt or a commercially available can be used without a like number, leaf, root, stem and is enabled to any center, while not limited to the use of the present invention preferred embodiment of plant leaf or leaves but most preferably is of the triple-eastern inside muscle connection.

In said method, step 1) extraction of the solvent can be water, alcohol, organic solvent or a mixture of these and the main preferably. Said alcohol include C₁ To C₂ Use is preferably lower alcohols, lower alcohols include ethanol or methanol preferably using. It freely extraction extraction method, using Soxhlet extraction or reflux extraction preferably but not limited. Said leaf or root powder dose 1 to 30 times while three extraction solvent preferably added to extraction, extraction 20 times more preferably added. Preferably 20 °C to 100 °C in extracting temperature, more preferably in 20 °C to 40 °C, but is most preferably an room temperature, not limited. In addition, extraction time is 10 to 48 is preferably a few times, and more preferably in a time 15 to 30, most preferably an 24 time but, not limited. In addition, extraction is preferably the number 1 to 5 it is a sliced raw fish, more preferably 2 to 4 times extracting, or most preferably it is a sliced raw fish 2, limited to are not correct.

While 95% and preferably 40 to extract said three main extract, more preferably 50 to 90% is a main extract, a main 60 to 80% and most preferably an extract, said 40% hereinafter extraction efficiency is lowered in the case of a main extract target hangover effect and may be, 80% main extract, while the triple-extract cost of break manually his fellow workers.

While three said extract has dehydrogenase and aldehyde dehydrogenase characterized in that interaction.

In the embodiment of the present invention in particular, the present invention while the victims of the number of leaf and root portion 70% using fermentation extract have three main high pressure liquid coolant, the same HepG2 hepatocyte cell lines when it has processed, while three said alcohol dehydrogenase and aldehyde dehydrogenase extract check at the time of interaction (also reference 1), while three of the present invention extract can be extracted non-hangover.

As health food of the present invention either adding food or food component while three can be use with other extract, can be suitable for use in accordance with the conventional method.

Said type of health food is simplified and a smooth free number. Performance examples of food while three said meat, sausage, bread, chocolate, candy, [su[su] neck type, confectionery, pizza, if, other noodles, gum type, ice cream acid number a dairy product, various sprocket, beverage, difference, drinkable number, alcoholic drinks and vitamin composite number and the like, health food comprising both in conventional meaning.

Any number or natural carbohydrate of the present invention is conventional beverage and lower health functional sound give excellent flavor can be the active ingredient. The above-described natural carbohydrate glucose, oligosaccharides such as mono pathogenic agent, liver, such as shoe cross andd company Kara id, and dextrin, cyclodextrin such as polysaccharide, xylitol, sorbitol, such as processes for the preparation of party alcohol[thol[thol] among others. As sweetener number TAU denaturing detergent, such as Stevia extract or natural sweetener number, saccharin, etc. such as synthetic sweetener number oh [su[su] group [lu[lu] with burning, can be. The ratio of said natural carbohydrate of the present invention per 100 g composition generally about 0. 01 to 0. 04 g, preferably about 0. 02 to 0. 03 g are disclosed.

In addition to the health food of the present invention said various nutritional number, vitamin, electrolyte, flavor number, colored number, [pheyk[pheyk] The [thu[thu] it buys and salts thereof, alginic acid and salts thereof, organic acid, protective colloidal thickening number, pH control number, stabilizing number, antiseptic number, glycerin, alcohol, number of calcium hydroxide can be used in carbonated beverages and the like. In addition to natural fruit juice, fruit juice beverage and vegetable beverages containing pulp can be of number for numerical control machine. These components can be used independently or mixing. The triazines of the present invention composition 100 parts by large number but not significant added ratio of 0. 01 to 2 parts by weight of generally is selected from the range are disclosed.

Hereinafter, the present invention embodiment in the embodiment and experiments by a detailed as follows.

Stage, in the embodiment for the present invention is exemplified ephemeral embodiments and experiments, in the embodiment of the present invention and experiments for contents are not correct limited on one end.

<In the embodiment 1> While three Extract number bath

The group consisting of agricultural techniques whereby northern provincetotal nudity while assessing the identified three purchase a morphological center facia, while three have washed leaves after freeze-dried (PVTFD 10R, Ilsin Lab, coupled in, a compensation), crusher using uniformly crushing, 20 times 70% to 100 g sample applying a predetermined main fermentation, periodical repetition time 24 at room temperature by 2 to his agitation extraction (Jeio tech SK2 provided 71, Lab companion, charged, a compensation). Extract ADVANTEC paper (No. 6) (ADVANTEC Co. , Tokyo, Japan) have conduit, pressure sensitive concentrator (EYELA N-a 1000, Riakikai Co. , Ltd. , Tokyo, Japan) was used after using concentrated lyophilized experiments.

<Experiment example 1> While three Extract by alcohol dehydrogenase (AlcoholDehydrogenase, ADH) And aldehyde dehydrogenase (aldehyde dehydrogenase, ALDH) confirmation of activity

<1-1> Stem Homogeneous liquid Obtained

The method of said<in the embodiment 1>alcohol dehydrogenase and aldehyde dehydrogenase activity in stem cells while three prepared by the number extract are isolated by the method for identifying whether hepatocyte homogeneous solution is obtained.

Specifically, a hepatocyte cell line (ATCC hB provided 8065) 10% (v/v) heat deactivated small [thay[thay] HepG2 oh serum (fetal bovine serum, Gibco, U.S.) and 1% penicillin-resistant Streptococcus pneumoniae (penicillin-a streptomycin, Gibco, American) included in the erythromycin (v/v) Streptococcus - MEM medium (Gibco, U.S.) to 1 × 10⁵ The dog after to form, by which is included in the 24 - well plate 24 hours 1 ml him as. And, a stand-alone plate culturing cells and said number of media, media<in the embodiment 1>(controls), while the triple-roots and leaves extract after four magnetic 1 ml, 5% CO₂ , 37 °C 24 hours after culturing conditions, extract of said plate and a stand-alone solution number 0. 25% trypsin - EDTA 5 minutes by adding 1 ml 37 °C (trypsin non-eDTA, Gibco, American) by reacting to a hepatocyte that were unsafe. Said recovered found out that 0. 1 M of PBS buffer solution (Gibco, American) during centrifuging to 1 times after washing, washing cells by adding PBS buffer solution of 300 micro l after mixing, it is possible to 2 mm bead (bead) vial (vial) was divided. 10 seconds using said fast prep -24 egg-(Fast prep provided 24) 4 times iteration has been homogenized, homogeneous liquid centrifugal through separation of supernatant of hepatocytes are obtained.

<1-2> Alcohol dehydrogenase and aldehyde dehydrogenase active confirmation

Said experiment example <1-2>Homogeneous solution obtained in alcohol dehydrogenase and aldehyde dehydrogenase in hepatocytes while solid imaging is processed in order to identify the activity of protocol of the radiation as it is analyzed using the same assay kit using absorbance number was.

Specifically, 96 - well said experiment example <1-2>Stem 50 that has been obtained in homogeneous solution by dispensing a micro l, using absorbance assay kit (Biovision, American) presents a number misfortune Zion mix (Reacion mix) behind in mixing, reacting 37 °C to 450 nm were measured absorbance in 30 minutes. Alcohol dehydrogenase and aldehyde dehydrogenase activity calculated expressions 1 and 2 of fig. 1 method to expressions such as upon her.

[Mathematical equation 1]

Active=(ADH or reaction time (min)/(mol) × (mL) sample capacity be ALDH mole) × dilution drainage

[Equations 2]

Active/active regulated relative active=experiment group × 100

As a result, as shown in the root extract while also 1 alcohol dehydrogenase and aldehyde dehydrogenase of the triple of leaf and significantly activating action of neutrophils (also 1).