SKIN WHITENING COMPOSITION USING EXTRACT OF LIMONIUM TETRAGONUM
The present invention refers to around [kays[kays] qualities ( The interior of the body from the environment of our various external skin protecting the most outside a body organ while the largest engine we are disclosed. The bottom of the keratinocytes (keratinocytes) number in these skin billion, or macrophage (macrophage) (Langerhans cells) skin immune is responsible for with gel one [su[su] cell, enter the dermal fibroblast (fibroblasts), and the like present oligonucleotide which is responsible for generating pigment (melanocytes). Melanin is a biomacromolecule can be an oligonucleotide synthesized from exterior environments of UV black skin protects cells which serves to protein complex are disclosed. However human melanin generating an excess of melasma, ephelis or the like and can even cause skin cancer form (J. P. Ortonne and J. J. Nordlund, The pigmentary system: physiology and pathophysiology, eds. J. J. Norlund, R. E. Boissy, V. J. Hearing, R. A. King and J. P. Ortonne, 489, Oxford University Press, New York, 1998). The melanin is present in proteins involved in number and it is sour with the mote but Ohit is sour with the mote but Oh oligonucleotide number generated by the action of (tyrosinase related protein). Specifically it is sour with the mote but Oh number (tyrosinase) by tyrosine (tyrosine) to be used as a waveguide (DOPA, 3, 4 a-dihydroxyphenylalanine), ear waveguide it is a jade city number as a function of the waveguide into a waveguide quinone (dopaquinone) (dopaoxidase) after being oxidized again, it is sour with the mote TRP-a 1 number related protein (5, 6 a-dihydroxy indole-a 2 non-carboxylic acid oxidase) and TRP provided 2 (dopachrome tautomerase) generated by the action of. Number it is sour with the mote, transcription factor MITF (microphthalmia non-associated transcription factor) TRP-a 1 and TRP-a 2 is expressed by is promoted, MITF is ERK (extracellular signal-a regulated kinase) is activated if a billion over known number expression (DS Kim, et al. , J Cell Sci. 2003;116: 1699 - 706). Generating a first step number participates to it is sour with the mote but Oh since melanin for inhibiting a second operation end studies find number number number whitening it is sour with the mote but Oh immunohistochemical studies (Kor. J. Pharmacogn. , 44 (3): 220, 2013). The majority of the current number and number are employed primarily whitening number such it is sour with the mote but Oh inhibitor, hydroquinone (hydroquinone) (arbutin, beta-D non-glucopyranoside) in which it is glycoside is arbutin, kojic acid (kojic acid) having inhibitory activity on both it is sour with the mote but Oh number like whitening number are disclosed. Hydroquinone (hydroquinone) is arbutin glycoside in which it is tolerable number it is sour with the mote but Oh (arbutin, beta-D non-glucopyranoside) a substance inhibiting the activity and, kojic acid (kojic acid) number it is sour with the mote but Oh chelating activity to tyrosine in the waveguide, waveguide quinone progressed into a substance inhibiting the process are disclosed (Korean J. Plant Res. , 26 (5): 526, 2013; J. Korean Soc. Food Sci. Nutr. , 41 (2): 156, 2012). However said whitening number number number is long-term in use both these synthetic whitening synthetic whitening is dermatitis, carbostyril derivatives for, back counter evidence, like the side effects of have, such as Taiwan some countries medical expert defined an acceptable concentration etc. available without prescription. Synthesis whitening human skin irritation and side effects to the safety door number number of these cookies to relatively small natural genuinely looking for extended studies in whitening material progressing disclosed. 2 Na-friendly salt lifestylearound [kays[kays] qualitieswith in [kays[kays] qualitative wondermentsyear lifestylethe seashoresalt lifestyle NaOCl in a rechargeable and high extraction process into plants belonging (Korean J. Ecol. , 13:9 - 18, 1990; Korean J. Ecol. , 6:167 - 176, 1983), forming a number of offshore the southwest gene in our area near room side guide seven cotton seconds, it does, old Mr. the herbs, etc. [kays[kays] grasses phones distribution (Korean J. Ecol. , 13:9 - 18, 1990; Korean J. Ecol. , 6:167 - 176, 1983). For example greater strength from the root [kays[kays] qualitative respects (Ocean Research 20:167 - 178; 1998) and NO3, health food or functional cosmetics to physiologically active ascertained [kays[kays] qualitative respects if a high safety is inserted into the can. The present invention refers to melanin formation billion number based on skin whitening activity around [kays[kays] qualities extract number as the use of such disclosure as follows. The purpose of the invention is using chrysanthemum extract or a reversed F. number under public affairsaround [kays[kays] qualities skin whitening composition. Another object of the present invention will hereinafter or specific number is in when are disclosed. In the embodiment in the present invention refers to as identified below and experiment, the mouse melanoma cell lines in alpha-MSH around [kays[kays] qualities extract melanin formation induced number be processed depending on the stimulating melanin billion number which the activity concentration when B16F 10 cells, particularly cytotoxic do not show a finished by S. confirm it. Based on the result of the present invention refers to this experiment so that ball number, extract of the present invention composition for skin whitening comprising around [kays[kays] qualities characterized. In the specification, "around [kays[kays] qualities extract" RM extraction formula be around [kays[kays] qualities, stem, leaf, fruit, flower, root, water or a mixture of these, methanol, ethanol, butanol 1 to 4 carbon atoms such as alcohol, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, N - dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), 1, 3 - butylene glycol, propylene glycol extract obtained by using a solvent or a combination of cholesterol, carbon dioxide, supercritical fluid extraction using a solvent such as pentane extract obtained above or extract thereof seeds which may be obtained by means, the polarity of the active substance extraction method, extracting degree, degree preservation by considering leucorrhea needle, reflux, heating, ultrasonic radiation, any schemes be supercritical fluid extraction or the like. After fractionated extract said extract by suspending particular solvents other mixed with a solvent polarity, being the fraction which is obtained, said extract hydrophobic solvent adsorbed on the column filled with silica gel or the like, obtained by mobile phase solvent or a combination of hydrophilic solvents as meaning including fractions are disclosed. In addition said meaning of the extract is lyophilized, vacuum drying, hot air-dried, spray drying of solid extract number extracted solvent liquid extract or concentrated for reparing over multiple myelomas are included. Preferably water as an extraction solvent, using a solvent or a combination of ethanol extract obtained above, more preferably extraction solvent ethanol extract obtained using a solvent mixture of water as big. Also disclosed in the specification, "active ingredient" RM alone the desired activity or itself with active ingredient carrier and of big. Also disclosed in the specification, "skin whitening" excess melanin degradation will increase in brightness can be decreased skin, specifically the excess melanin generation a melasma, freckles, caused by biotin or (reducing conditions), treatment, prevention, expression billion by big number or delay. In addition to the active ingredient of the present invention composition for skin whitening, reinforcing skin-related activity, publicly known skin whitening component to add additional contrast, skin wrinkle, ultraviolet care constituent, such as skin-moisturizing ingredients including number bath 1308. Specifically skin whitening component as the arbutin, age it knows the id, ascorbate bill writing base nose side, alpha - step roll nonuse, extract (Extract Oil Soluble Licorice (Glycyrrhiza)) and as to the utility, the skin wrinkle as the retinol, retinoic neel sells the American reel the [thu[thu], adenosine, polyethoxylated amide such as cited, as the draw maul tree it will doze ultraviolet care constituent, draw maul tree the tree thread rock which will doze it buys, d it will go with the tree which rises a [thu[thu] which comes, seven cuts in the D maul Coordinator well at end four the [thu[thu], to D as much as seven [heyk[heyk] thread hit America tree Oh zone, pine tree extract such as composite, phosphatidylserine, finger root extraction powder, whereby mixed extract of red Ginseng and the like is cited. The moisturizing agent uses AP collagen peptide enzymatic degradation, Collactive collagen peptide, N - acetylglucosamine, an extract of an auxiliary device, such as a simple mixed extract, rice bran extract, corn germ extract, collagen peptide, lithospermum erythrorhizon extract powder, phosphatidylserine, hyaluronic acid is cited. Other skin whitening component, wrinkle, ultraviolet care constituent, skin-moisturizing ingredients pdxl cosmetic method according to functional cosmetics lost motion ("functional cosmetics reference and test method" notice wife about), health food laws health food lost motion (notice wife about " reference and standard health food) can be reference. Such components are present in skin whitening composition comprising the heater of the present invention with at least one validity thereof can be. In skin whitening composition of the present invention, the active ingredient and use, according to the type number of human toxicity without affecting any amount within a range that can be, specifically relative to the total composition weight relative to 0. 001 to 99% by weight. 99% by weight can be included. In one aspect of the present invention specific composition for skin whitening, cosmetic composition can be grasp. Cosmetic composition of the present invention composition information even when cosmetic composition its use as a matter of, law any of the number article can be divided band, in particular tyrosinase functional cosmetic skin uses, be a non-functioning general cosmetics or the like. Any number can be provided in the form article even number article form, specifically solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, number - surfactants containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray or the like number elder brother anger 1308. Specific number form flexible article in water, nutritional skin water, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder be a number of glass. Cosmetic composition of the present invention in addition to the active ingredient and cosmetic composition components typically used, for example, stabilizing number, solubilized number, number surfactants, vitamin, such as clause [lyo[lyo] and dye and conventional auxiliary number, can be and a carrier. Of the present invention number type paste, cream or gel carrier ingredient when animal oil, include esters of fatty acids, wax, paraffin, starch, coating space [thu[thu], cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide or the like can be used. This powder or spray when a carrier ingredient of the present invention number type lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used, in particular spray case additionally chloro [phul[phul] Base Oro draw carbon, propane/butane or dimethyl ether can be such as to expel the projectile. When the emulsions of the present invention number type solution or solvent carrier ingredient, solubilized number or number is used device for cleaning, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3 - butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, such as sorbitan fatty acid esters can be used. Of the present invention number type suspension carrier ingredient when water, such as ethanol or propylene glycol liquid dilution number, box-ethoxylated alcohol, polyoxyethylene sorbitol esters, such as polyoxyethylene sorbitan ester suspension number, microcrystalline cellulose, aluminum meta [tu[tu] rock hour [tu[tu], bentonite, agar and the like can be used. The interface type of the present invention number number carrier ingredient aliphatic alcohol containing cleansing when active - sulfate, aliphatic alcohol ether sulfate, sulfonyl succinic ester, cleanser, imidazole [nyum[nyum] derivatives, methyl tau [ley[ley] [thu[thu], cooperates with the disadvantages, fatty acid amide ether sulfate, alkyl polyglycoside, aliphatic alcohol, fatty acid glyceride, fatty acid d ethanol amide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester or the like can be used. Cosmetic composition of the present invention the active ingredient to the skin whitening activity including number and those in the cosmetic composition according to the manufacturing method number under the outside contrast typically can be high pressure liquid coolant. Skin whitening composition of the present invention these compositions can be used specifically in one embodiment, food composition can be grasp. Any number as food composition of the present invention can be tank, e.g. tea, juice, carbonated beverage, beverage flow ion drinks, milk, request route of a machining oil, gum type, rice cake, lemon, bread, confectionery, fixing food, positive number, capsule, ring, granules, liquid, powder, flaky, pasty, syrup, gel, jelly, the number of bar health food like number number 1308. bath. The food composition of the present invention on law, in electromagnetic number tank sections, distribution point convenient law enforcing any number article can be divided band. For example health food or health food according to law, food the hygienic law (wife about highly, reference and standard food) each food type according to current food lost motion on soybean milk, fermented beverage flow, be a spacial use foods. In addition to the active ingredient of the present invention is provided with a food additives can be included in the food composition. Food additive is generally food number tank, processing or preserving filled by infiltrating it will as to be combined be added to, taken daily with food refresh should be ensured so that its safety. According to food additive lost motion (wife about highly, food additive criteria and standard) the hygienic law food safety is guaranteed food additive is chemical synthetic width, natural additive, mixed number number current in the order of exclusively defined disclosed. These food additive relates sweetener in the metal number, flavor number, preserving number, number emulsion, containing at least one, such as thickening can be divided into a number. The sweetener number is appropriate sweetness or can be used in a quantity of food, can be natural may be synthetic in nature. Preferably the other party using natural sweetener number, corn syrup solids as natural sweetener number, honey, sucrose, fructose, lactose, maltose sugars such as a sweetener number is cited. Number that can improve the flavor is used to improve the aroma can be, can be both natural and synthetic. Preferably when natural people are disclosed. In addition to the range of natural flavor when the aim of nutrition can be also in parallel. Number natural flavor as apple, lemon, citrus, grapes, strawberry, peach or the like obtained or popsicle, Solomon's seal, large leaf, cinnamon, chrysanthemum leaves, be a character it soaked or the like obtained. The Ginseng (red), antibacterial, aloe vera, using money obtained in disapproval. Natural flavor concentrate or be a solid extract is number of 8. Flavor can be used when the second channel number, synthetic flavor number is ester, alcohol, aldehyde, terpene or the like can be used. As preserving number the [pu[pu] it buys the calcium sodium sorbitol, sorbitol the [pu[pu] it buys natrium, sorbitol the [pu[pu] it buys the potassium, benzoic acid calcium, benzoic acid sodium, benzoic acid potassium, EDTA (weathering) can be is used as the alkali, as gum acacia in emulsion number, carboxylic diplopia methyl cellulose, a thickening, and as to the pectin, as containing at least one operation, malic acid, fumaric acid, adipic acid, phosphoric acid, a central, tartaric acid, ascorbic acid, acetic acid, is used as the phosphate can be. Containing at least one taste enhancing proliferation of microorganisms in addition number billion food composition is the purpose can be added so that the titratable acidity. As suspension pads since number number, precipitated number, gel formation number, is used as the solubility number can be. In addition to the food composition described above of the present invention generate, nutritive supplement functionality, as food additive and guaranteed stability for purposes of reinforcing contrast publicly known can be physiologically active substance or mineral acid. A novel physiologically active substance such as a green tea catechin, vitamin B1, vitamin C, vitamin E, such as vitamin B12 vitamins, tocopherol, dibenzo one thiamine can be a bearing, such as a nutritional calcium number number as citric acid calcium, magnesium salt of stearic acid such as magnesium number number, citric acid iron number number like iron, chromium chloride, iodine potassium, selenium, germanium, vanadium, process for recovering is cited. Food additives of the present invention food composition described above achieves the purpose comprising a wall according number article types can be added. A food composition of the present invention generate idle or food additive may be included with respect to reference other food can be lost motion. In one aspect of the present invention specific for skin-whitening composition, therapeutic composition can be about number information. Object of the present invention therapeutic composition when with pigmentation symptoms skin whitening composition of about number information can be understood to skin diseases, such with pigment needle work symptoms specific one example of freckles, age spots, [...], melasma, brown or NaCI, sunlight dye semi, fattened black blood symptoms (cyanic melasma), number calcium antagonists such as drug utilization of excess pigmentation, tissue curing therapy (sclerotherapy) according to sequelae, pregnancy-brown semi (gravidic chloasma), take oral contraceptive use in female black blood symptoms, wound or dermatitis after inflammation due to excessive scratch and Image including pigmentation, toxic reactions or other similar light but like fixing apparatus for PHOTOGRAPING pigmental formed integrally, the number one are not disclosed. In addition to the compositions of the present invention about number contains lysophosphatidic acid is publicly known conventional method including a cosmetically acceptable carrier about number contrast administration or parenteral number number number bath-oral along path can be disclosed. Wherein the meanings of effective ingredient activity "about number the cosmetically acceptable" number billion (prescription) applied without toxicity of non-adaptable or more meaning that object are disclosed. Oral pharmaceutical composition of the present invention about number-number number bath when excited, contrast with the publicly known method suitable carrier according to powder, granules, positive number, ring number, number dragee, capsule number, number liquid, gel number, number syrup, suspension, when the arm-number number bath 1308. The cosmetically acceptable carrier of one example of a suitable about number lactose, glucose, sucrose, dextrose, sorbitol, mannitol, xylitol of saccharide, corn starch, potato starch, wheat starch before classification such as, cellulose, methyl cellulose, ethyl cellulose, sodium carboxymethylcellulose, hydroxypropyl methyl cellulose and the like current of cellulose, polyvinyl pyrrolidone, water, methyl hydroxy benzoate, propyl hydroxy benzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, include esters of fatty acids or the like is cited. Number number number anger bow needed when filling, a specific number, number coupled, wet number, number disintegrating, and/or excipients such as surfactants can be under anger including dilution number number number number number. Number-number number parenteral pharmaceutical composition of the present invention about bath when excited, contrast with the publicly known method suitable carrier between the first and second number, number transdermal administration, nasal inhalation in the form of number and number of left number number can be disclosed. To be suitable as carrier when scanning number number number anger bow sterilization, ethanol, glycerol or propylene glycol such as blank interval and polyol or mixture thereof, preferably ringer solution, PBS (phosphate buffered saline) containing high pressure sterilization or be for injection, such as 5% dextrose and appearing in the solution and the like can be used. When the transdermal administration number number number number under anger ointment, cream number, number lotion, gel number, number external solution, pasta number, candle [ni[ni] number, like in the form of number number of air roll number can be disclosed. In the case of nasal inhalation number dichloro chlorofluoromethane, with [phul[phul] base Oro methane production, dichloro tetra [phul[phul] base Oro ethane, such as CO2 using aerosol spray form can be of suitable propulsion number number number, number for number include above [theyp[theyp] brush left (witepsol), twin (tween) 61, polyethylene glycols, carcass five fingers, the it soaked, of polyoxyethylene sorbitan fatty acid esters, polyoxyethylene [su[su] reel Oh [ley[ley] [thu[thu] type, sorbitan fatty acid ester or the like can be used. With the publicly known about number number number of contrast with respect to compositions which, specifically synthesized [Remington's Pharmaceutical Sciences (19th ed. , 1995)] or the like can be reference. Said document describes a are deemed as part of the specification. Compositions of the present invention about number of the preferred dosage to a patient's state, weight, sex, age, the severity of a patient, according to routes 1 0. 001 mg/kg - 10 g/kg range, preferably 0. 001 mg/kg - 1 g/kg may be in the range disclosed. Administration agent 1 1 can be made into many times. The valve timing of the way of the present invention range in number are not any side such dosage is interpreted. As aforementioned, according to the present invention can be under public affairsaround [kays[kays] qualities skin whitening composition using chrysanthemum extract number. Skin whitening composition of the present invention in food, cosmetic, drug or the like can be width number, greater in number around [kays[kays] qualities root which stores data when compared to the synthetic skin whitening relatively effective component thereof can. Figure 1 shows a mouse melanoma cell lines also around [kays[kays] qualities extract is the highest concentrations in B16F 10 cells 200 micro g/mL schizandrin indicate processing result are disclosed. Figure 1 number stimulating mouse melanoma cell lines in alpha-MSH around [kays[kays] qualities extract melanin formation stimulating melanin B16F 10 is shown having a billion number activity result are disclosed. Hereinafter the present invention in the embodiment and experiment through a browser onto a substrate. In the embodiment of the present invention however this embodiment and not the limited range and experiment. <In the embodiment> around [kays[kays] qualities Extract number bath around [kays[kays] qualities (Structural formula) Powder 10 times weight of 70% ethanol at room temperature was found 24 applied time is then filtered. The obtained extract around [kays[kays] qualities filtrate cream or freeze-drying. <Checking your> <Experiment example> around [kays[kays] qualities Extract of whitening active confirmation <1> Experimental method <1-1> Cell culture Mouse melanoma cell line (melanoma cell line) in B16F 10 cells (Korean Cell Line Bank) when the purchase from Korean cell line bank, 1% antibiotic number (Gibco, USA) and 10% serum (FBS; Gibco, Grand Island, USA) and right [thay[thay] DMEM medium (Dulbecco's modified Eagle's medium) containing using 37 °C, 5% CO2 When the cultured in incubator, into the embodiment 4 are excellently at a predetermined force. <1-2> Cytotoxic evaluation In the embodiment of the test sample for measuring conducting MTT analysis influence on cell growth. Metabolic abounding in living cells, intracellular mitochondrial dehydrogenation of enzymatic action is by a water-soluble yellow MTT (3 - (4, 5 a-dimethylthiaxo provided 2 a-yl) - 2, 5 a-diphenyl tetrazolium bromide) (Sigma, MO, USA) in a water-insoluble formazan (formazan) a reduced purple 72 formed on the substrate. 2 optimum medium DMEM 10% FBS added B16F 10 cells. 0 × 104 In the embodiment according to sample cells/mL 96 well plate is placed 18 treating him as time 48 concentration culture nor post-incubation time. After 4 hours MTT solution by adding 50 micro l been reacting. L applied and removed with dimethyl sulfoxide (dimethylsulfoxide) culture medium alone completely number 200 in completely dissolving the precipitate after a micro, using 540 nm absorbance microplate reader (microplate reader) were measured. Each sample average absorbance value is found through a pulse group, controls of absorbance compared to cell lifestyle market rate was assessed. <1-3> Melanin biosynthesis inhibitory activity measurement B16F 10 cells are cultured in DMEM medium 2 a 24 well plate. 0 × 104 Cell/well of alpha-MSH culture nor post-incubation time is 100 nm (- melanocyte stimulating hormone) 24 and reliably on a sample number in the embodiment according to bath concentration off him as time 48. Next phosphate buffer (pH 7. 4) washing the lysis buffer (100 mm sodium phosphate (pH6. 8), 0. 1 mm PMSF, 1% Triton X-a 100) with respect to the dissolved in 30 minutes -80 °C into and out of the cells. Applying an 80 °C 1N NaOH then melt cells 1 in the temporal sediment after dissolving in absorbance for improving 405 nm were measured. <Confirming our experiment> Melanin biosynthesis inhibition nothing addition old friendship absorbance reduction shown for sample solution added to port. Arbutin (Arbutin) regulated as well as the electrode. <2> Experiment result <2-1> The result of measurement of cytotoxicity B16F 10 cells around [kays[kays] qualities extract (200, 100, 50 micro g/mL) processing an output of the cell survival rate after 48 culturing time MTT results to identify [1 also] have shown. Reference [1 also] induced by solar heat, and do not show the highest processing around [kays[kays] qualities extract 200 micro g/mL concentrations can be cytotoxic even confirm it. <2-1> Measuring result melanin synthesis Evaluating a mouse melanoma cells to be damaged around [kays[kays] qualities extract natural whitening number around [kays[kays] qualities B16F 10 cells extract (200, 100, 50 micro g/mL) and melanin formation induced a number in alpha-MSH off, induced by alpha-MSH billion number measured by the degree to which the melanin production around [kays[kays] qualities extract, [2 also] have shown to result. Reference [2 also] induced by alpha-MSH around [kays[kays] qualities extract produced by induced by melanin concentration dependent 15 to complete the transaction. 5%, 26. 94%, 30. Identifying reducing can be 85%. Disclosed is a skin whitening composition using an extract of Limonium tetragonum, which shows the melanin production inhibitory activity in a concentration-dependent manner when being treated on melanoma cell line B16F10 cells of mice stimulated by α-MSH, which is a melanin production inducer. The extract of Limonium tetragonum is obtained by using water, ethanol, or a mixed solvent thereof. The skin whitening composition of the present invention can be commercialized into foods, cosmetics, medicines, etc., and can have relatively less side effects compared to synthetic skin whitening agents since Limonium tetragonum roots have been used for edible purposes. COPYRIGHT KIPO 2017 The active ingredient around [kays[kays] qualities extract skin whitening composition. According to Claim 1, said around [kays[kays] qualities extract water, ethanol or a combination of skin whitening composition characterized by a solvent obtained. According to Claim 1 or Claim 2, said skin whitening composition comprising a cosmetic composition which composition. According to Claim 1 or Claim 2, food composition which skin whitening composition comprising said composition. According to Claim 1 or Claim 2, characterized in that said composition has a therapeutic composition which number skin whitening composition.
