METHOD FOR SEPARATING SUCROSE BY SELECTIVE CRYSTALLIZATION
The present invention refers to sucrose (sucrose) to selective crystallization separation and method are disclosed. Environment (green solvent) (ionic liquid) in relation to the characteristics of an ionic liquid as solvent, ionic liquid having a melting point of 100 °C hereinafter generally isolated and conductive rubber as silica salt thereof is defined as follows. Ionic liquid 100 years ago unique known to form an excess of intensity but intensive studies for making the composite kind not been provided. This is because the ionic liquid in which synthesis as an ion implantation mask, in addition of an ionic liquid didn't's oldest importance on him. However 16 February 2005 [kyo toes linkable fermented [...] by world in a reaction substances, use and transmits movement of number of volatile organic solvents to replace [...] beginning with an ionic liquid solvent (green solvent) receives interest in environment-friendly study began. This number of [...] due to environmental contamination as a result of the social pressure can be called. Ionic liquid cation and anion elements structurally unique and liquid, only their properties while maintaining a respective independently action differ from that it so that a common organic and inorganic solvent. General of an ionic liquid characteristics include high thermostable, non-volatile (low vapor pressure), high solvating organic and inorganic material, are solvates the silver ions are not, to 400 °C wide liquid range, high polarity, etc. high ion conductivity. In addition 10 with theoretically18 Degree of cation and anion is injected into the existing combination of physical and chemical properties can be implementing organic and inorganic solvent is replaced or novel properties can be by virtue designer solvent is called substrate. The ionic liquid using solid-state isolation technique when applied to crystallization, crystallization separation technique in the range wider than general organic and inorganic solvent variables and can design the...copyright 2001 (also 1). On the other hand, with respect to the selective crystallization (selective crystallization) through isolation technique, excess solvent solute in trace amounts of carbon monoxide in addition to the two components equivalent amount if the larger angle based often be coexistence. In this case two equally according to the balance of relationship (phase diagram) revealing the upper criticism type melting and liquid metals also are disclosed. The elder brother degree upper criticism equilibrium relationship can be divided into solid solution orgin eutectic-based (eutectic system) conducted to on (solid solution system). When eutectic systems such as temperature and composition of the mixture of two components as in Figure 2 the molten at have the properties described. About both sides of the component on a separate solid state only that the area crystallized eutectic is present. In the case of larger angle ADC region is mixed liquids of solids and A/B - A - while, in the case of BCE region is provided to mixed liquid of solids and A/B - B - 2000. In determining the amount of amount of liquid is present in zero misfortune of a worker laws can be calculated simply. I.e., the ratio of the mixture body temperature and composition in pure solid A M amount amount A/B - ML: SM length segment such as disclosed. When the solid is generated according to a pure eutectic systems of those components is the selective crystallization (selective crystallization) region where crystallization process for the component separating permits. In the case of two solid solution orgin (also 3) permanent magnet region in composition of solids and liquids (A/B) is provided to each other. AL ': liquid line and LB bilinear AS': SB solid boundary line are disclosed. In M before this reaches the liquid when subjected to a voltage on the boundary line exists in a liquid state before composition, in L encoded deposit crystals for the first time. The composition of the composition are the same as upon S point are disclosed. A lower temperature compensated by precipitating the solid composition of solids from a liquid composition of mobile left along boundary line S point along and moving left liquid boundary line, the composition of liquid when temperature reaches L S compound substrate. (Co-a crystallization) is based crystallize ball - and through the selective crystallization (selective crystallization) purity as crystallization separation permits. And, natural selective crystallization separation of active substances in conjunction with high efficiency and high quality need, before world current medical interest and support may be replaced (Complementary and Alternative Medicine: CAM) concentration, trained along traditional, such as traditional public opinion crane based on natural active substance reduces global in medical studies been before. In order to obtain the active substance derived from natural but traditionallyExtraction, separation, and is a positive number process, in order to obtain the various apparatus and method mated as active ingredients from the remote. However this traditional method is the read and write equipment, manpower to drive continuous process have been difficult to limit. In addition pharmaceuticals suitable for use in active material separated in addition does not significantly lower the quality of the hard mask is in many cases been measured number of the doors (4 also). A plurality of papers and patent document specification reference throughout its citation displayed disclosed. The citation of the specification as a whole is inserted into the present invention disclosure content is referenced papers and patent document is provided to the levels and of the present invention content more specifically described. Victims of the method of the present invention tried to separate the active substance effective integrated dairy products. As a result, dissolved in an organic solvent radix, use of at least one ionic liquid [...] (anti-a solvent), volcanic rock through pure disaccharide is sucrose (sucrose) crystallization (drowning provided out crystallization) can be identifying selectively crystallization separation, finished the job of the present invention. Thus, the purpose of the invention is a selective crystallization separation method or a reversed F. number [...] sucrose (sucrose). Another object of the present invention and the advantage of the following detailed description of the invention, claims and drawing by more specifically with each other. According to one aspect of the present invention, the present invention refers to a method including a selective crystallization separation then sucrose (sucrose) number [...] other: (a) extracting Angelica gigas Nakai roots with an organic solvent; (b) adding said at least one ionic liquid on the resultant structure of (a); (b) and (c) said resulting induces crystallization of sucrose (sucrose) to obtain crystals. Victims of the method of the present invention tried to separate the active substance effective integrated dairy products. As a result, dissolved in an organic solvent radix, use of at least one ionic liquid [...] (anti-a solvent), volcanic rock through pure disaccharide is sucrose (sucrose) crystallization (drowning provided out crystallization) can be selectively crystallization separation has been confirmed. The specification "sucrose (sucrose) selective crystallization separation method" in the radix (preferably, radix root) among the components included in determining sucrose (sucrose) to selectively separate only as, "sucrose (sucrose) crystal number bath method" can be mixed and used. The specification terms in adding a high molecular weight of solubility change number 3 "volcanic rock (drowning a-out) crystallization" crystallization is derived are disclosed. The mixed with a solvent other [...] using (anti-a solvent), lowering the solubility inducing crystallization method by a goniophotometer. In the present invention said crystallization of a substance such as volcanic rock the crystallization law for applying solvent and organic solvent, number 3 material, i.e. semi solvent with respect to the ionic liquid for adding crystallization inducing and crystallization for subsequent analysis. Hereinafter, selective crystallization separation method of the present invention method in sucrose (sucrose) the detailed processes as follows: (A) Angelica gigas Nakai roots Organic solvent Extraction First, the organic solvent (a) extraction of roots pack of the present invention method comprising the following steps. The present invention belongs to the radix used in [...] ([...], Umbelliferae) (Angelica) inside jelly car into plant as, Korean satellite receiver ( Coumarin (coumarin) is composed mainly [...] lipid-soluble substance is soluble material which polysaccharides (polysaccharide), coumarin (coumarin) is anti-cancer (decursin), effectively treating (decursionol angelate), magnetron (umbelliferone) the bell lung freezing, it is roh [...] (nodakenin), the lung the rice field which is dominant (peucedanone), e maul scene (marmesin), dimethyl [su with hemp cloth new (demetylsuberosin), which like the lung being the toe phosphorus (isoimperatorin) isocyanate, one of the principle active ingredient composition for effectively treating the BST (decursionol angelate) (decursin) and converting them to differ. The present invention refers to the root of the main components of disaccharide sucrose (sucrose) to a user computer selectively crystallization separation are disclosed. According to an exemplary embodiment of the present invention, said step (a) radix 5 - 150% (weight/volume) and the root of organic solvents, more preferably 10 - 120% (weight/volume) which, 10 - 110% (weight/volume) than preferably are disclosed. According another embodiment of the present invention, said step (a) of the organic solvent is methanol, ethanol, butanol, isopropyl alcohol, pentane, hexane, heptane, cyclohexane, toluene, acetone, methyl acetate, methylene chloride, chloroform, ether, petroleum ether, benzene, ethylene glycol, propylene glycol and butylene glycol is selected from the group consisting of an organic solvent, more preferably methanol, ethanol, butanol, isopropyl alcohol, pentane and hexane selected from the group consisting of an organic solvent, preferably methanol or ethanol and than, most preferably methanol are disclosed. According to another embodiments of the present invention, said step (a) extraction of the temperature range from 20 to 28 °C and agitating time 30 in embodiment 5, more preferably 1 hr to 3 hours in 20 to 25 °C temperature agitating embodiment as follows. According to another embodiments of the present invention, said step (a) extraction of the 30 minutes time in embodiment 5 1 provided 35 °C temperature and agitating, more preferably 30 minutes agitating in embodiment 5 10 provided 30 °C temperature and time, than in embodiment 5 hours and 30 minutes agitating temperature preferably 20 provided 28 °C, 20 provided 25 °C 1 hr to 3 time more and more preferably temperature in agitating embodiment as follows. According another embodiment of the present invention, extraction of (a) prior to said step, said method comprising the following steps on which the root radix. Said milling various publicly known nitrile by breaking a physical method for example, shear (shearing), milling (milling) or grinding method and, mill, knife cutter, mixer, or [...] and using embodiment can close. (B) (A) addition of the resulting said ionic liquid Then, the resultant structure of (a) adding said at least one ionic liquid of the present invention method via substrate. According another embodiment of the present invention, at least one ionic liquid used in the present invention constituting cation may be an ammonium cation, imidazole bud cation, oxa the bud which will doze cation, d [nyum piperidinyl cation, it falls the bud antagonist cation, the bud which will doze antagonist cation, it hardens [nyum pyridyl cation, a pyridinium cation, pyrimido liquid-crystal medium cation, cation d [nyum pyrrole, pyrrole [...] cation, pyrrole bud cation, sulfonium cation, phosphonium cation and triazoles cations selected from the group consisting nicotineamid, more preferably ammonium halide cationic, imidazole bud cation, a pyridinium cation or phosphonium cation and, most preferably imidazole bud cation are disclosed. According to another preferred embodiment of the present invention, at least one ionic liquid used in the present invention constituting anion is ethyl alkylsulfate anion, tosylate anion, methane sulfone anion, tetra [...] anion, process fluorine phosphate anion, with the claw egg base this [thu which it pushes anion selected from the group consisting fluorine process into a solid carbonate anion and 2, more preferably 2 [...] anion, process fluorine phosphate anion, which process with the claw egg base this [thu which it pushes into a solid carbonate anionic or 2 fluorine anion, most preferably tetra [...] anion are disclosed. According another embodiment of the present invention than, the present invention 1 - ethyl - 3 - methyl ethyl [...] used in ionic liquid alkylsulfate, 1 - ethyl - 3 - methyl [...] tetra [...], 1 - ethyl - 3 - methyl [...] tosylate, 1 - ethyl - 3 - methyl [...] methane sulfonate, 1 - ethyl - 3 - methyl [...] free plate, 1 - ethyl - 3 - methyl [...] DC into amide, 1 - butyl - 3 - methyl [...] tetra [...], 1 - butyl - 3 - methyl [...] bis (triple release with neel methyl opinion gun) imide, 1 - butyl - 3 - methyl [...] DC into amide, 1 - butyl - 3 - methyl [...] triflate, 1 - butyl - 3 - methyl [...] methane sulfonate, 1, 3 - methyl - 2 - [...][...] bis (triple release with neel methyl opinion gun) imide, 1, 3 - d it will inform and already will be and the bud which will doze [...] 2, 1 - Butyl - 2, 3 - dimethyl [...] triflate, 1 - butyl - 2, 3 - dimethyl [...] tetra [...], 1 - (2 - hydroxyethyl) - 3 - methyl [...][...] 2, it will be burnt [...][...] 1 - 2 - 3 - methyl, 1 - butyl - 3 - methyl [...] MMP 2, MMP 1 - butyl - 4 - methyl 2 [...], 1 - allyl - 3 - tetra ethyl [...][...], 1 - bis (trifluoro methylsulfonyl) methyl -1 profile [...] imide, tree jade [thil ammonium methyl bis (trifluoro methylsulfonyl) imide, butyl tree methyl ammonium bis (trifluoro methylsulfonyl) imide, tree [...] d ethyl methyl opinion gun [nyum bis (trifluoro methylsulfonyl) bis (trifluoro methylsulfonyl) imide and selected from the group consisting of polyimide, such as 1 - ethyl - 3 - methyl [...][...] coating preferably than, 1 - butyl - 3 - methyl [...][...] 2, 1, 3 - d it will inform and already will be and the bud which will doze tetra [...], 1 - butyl - 2, 3 - dimethyl [...] tetra [...], 1 - (2 - hydroxyethyl) - 3 - methyl [...][...] 2, it will be burnt [...][...] 1 - 2 - 3 - methyl, 1 - butyl - 3 - methyl [...] MMP 2, MMP 1 - butyl - 4 - methyl - 3 - ethyl [...][...][...] or 1 - allyl 2 2, 1 - butyl - 3 - methyl [...][...] most preferably tetra (BMImBF4 ), 1, 3 - D it will inform and already will be and the bud which will doze [...] tetra (AAImBF4 ) Or 1 - allyl - 3 - ethyl [...][...] tetra (AEImBF4 ) Are disclosed. 4. [...] solutes dissolved in ionic liquid of the present invention method to solids (antisolvent). According to another preferred embodiment of the present invention, step (b) said step (a) organic solvents and said at least one ionic liquid volume ratio 0. 2 - 3:1 And, more preferably volume ratio is 0. 7 - 1. 5:1 And, most preferably volume ratio is 1:1. According to another preferred embodiment of the present invention, said step (b) said step (a) of ionic liquid pack of root for 100 - 10, 000 (volume/weight)% and, more preferably 300 - 5, 000 (volume/weight)% and, more preferably 500 - 3, 000% (weight/volume) are disclosed. (C) (B) sucrose (sucrose) obtained crystal of said resulting crystallization inducing and Finally, the (b) (c) of the present invention method induces crystallization of the resulting crystal sucrose (sucrose) comprising the following steps. According to another embodiment of the present invention, said step (c) said step (b) is left for 5 - 48 hours the embodiment to display the resulting crystallization of the derivatization and, (b) said step (c) more preferably said crystallization of the derivatization step aside time to display the resulting 10 - 24 embodiment with each other. At least one ionic liquid of the present invention employing a slim using except with anti-solvent, sucrose (sucrose) crystal obtained a better yield that the disclosed. According another embodiment of the present invention, said step (c) of said (b) induces crystallization of the sucrose crystals for obtaining resulting after the filtering method embodiment with each other. Said various publicly known filtration method embodiment in contrast can be, for example natural filtration (atmospheric filtration), filtering or pressurized filtration of embodiment but pressure method or the like, preferably of pressure filtration method. According another embodiment of the present invention, said step (c) of determining sucrose (sucrose) projections are 0. 5 Mm or more single crystalline and, more preferably length 0. 5 - 50 Mm single crystal are disclosed. According to another embodiment of the present invention, said step (c) sucrose (sucrose) (monoclinic system) (Å) of a seed crystal to the alkylation determination is=7. 751, B=8 (Å). 775, (Å) c=10. 804 Are disclosed. Said step (c) sucrose (sucrose) determination is more preferably further has a determining angle of α=γ=90° and β=103° in which, preferably than P21 In Space group further has a substrate. The abstract of the present invention features and benefits as follows: (A) sucrose (sucrose) selective crystallization separation method the present invention refers to a number [...] substrate. (B) integrated effective method to separate active substance of the present invention method of dairy products, radix dissolved in an organic solvent, use of at least one ionic liquid [...] (anti-a solvent), volcanic rock through pure disaccharide is sucrose (sucrose) crystallization (drowning provided out crystallization) can be selectively crystallization separation has been confirmed. (C) thereby, obtained disaccharide is sucrose (sucrose) determining and ensure stability of, the production yield in addition are improved. Figure 1 of an ionic liquid at or types and characteristics. Figure 2 shows a eutectic-based also in upper criticism type 2001 KIPO. Figure 3 shows a solution orgin aluminum upper criticism type 2001 KIPO. Figure 4 shows a positive number process also natural active substance separation and a mimetic 2001 KIPO. Figure 5 satellite receiver roots with anti-solvent BMImBF 4 ionic liquid using a selective crystallization split into a disaccharide (sucrose) of1 H-a NMR spectrum (solvent D2 O) at or. Figure 6 shows a satellite receiver roots also ionic liquid through the selective crystallization method using a mimetic of a thermal disaccharide (sucrose) are disclosed. Figure 7 Angelica gigas Nakai roots with anti-solvent precipitated disaccharide (sucrose) through the selective crystallization using ionic liquid crystal shape Image (Image pick times Video-a microscope 200) 2001 KIPO. Figure 8 of the present invention in the embodiment 1 to 3 ionic liquids in a disaccharide (sucrose) exhibits selective crystallization precipitation structure of the. Hereinafter, the present invention broadcast receiver through more detailed in the embodiment. The present invention is more specifically account for these in the embodiment is only, in the embodiment of the present invention according to the subject matter of invention number by one person with skill in the art that in these range-case is not in the art will. Throughout the specification, " %" is used to represent the concentration of a specific substance is referred if there is not a separate, solid/(weight/weight)% solids, % (weight/volume) solid/liquid, and liquid/liquid % (volume/volume) are disclosed. In the embodiment In the embodiment 1:1 - butyl - 3 - methyl 2 using disaccharide (sucrose) satellite receiver roots with anti-solvent [...][...] selective crystallization separation Using a satellite receiver root 500 mg was finely extending [...] and close. Even radix 5 ml methanol to finely put in the root 500 mg, 25 °C stirring in 2 time-gate. Pressure filtration of the stirring solution, solid number been stand-alone. 1 - Butyl - 3 - methyl [...][...][...] number is volatile solid solution 2 (BMImBF4 ) Equivalent to 5 ml, shake well after 12 hours at 0 length followed by filtration disaccharide (sucrose). A 5 mm or more single crystalline 20 mg obtained. In the embodiment 2:1, 3 - 2 with anti-solvent [...][...] selective crystallization separation using disaccharide (sucrose) satellite receiver roots The same method in the embodiment 1 is one embodiment, ionic liquid with anti-solvent, 1, 3 - [...][...] tetra (AAImBF4 ) As well as a. Disaccharide (sucrose) length 0. A 5 mm or more single crystalline 15 mg obtained. In the embodiment 3:1 - allyl - 3 - ethyl [...][...] using disaccharide (sucrose) satellite receiver roots with anti-solvent 2 selective crystallization separation The same method in the embodiment 1 embodiment is coated, with anti-solvent ionic liquid, 1 - allyl - 3 - ethyl [...][...] tetra (AEImBF4 ) As well as a. Disaccharide (sucrose) length 0. 5 Mm or more single crystalline 18. A 4 mg obtained. In the embodiment 4:1 - butyl - 3 - methyl [...] Chinese Angelica gigas Nakai roots 2 using disaccharide (sucrose) with anti-solvent [...] selective crystallization separation The same method is one embodiment to in the embodiment 1, as well as per the truth returning home a pack not a Chinese Angelica gigas Nakai. Disaccharide (sucrose) length 0. 5 Mm or more single crystalline 17. A 6 mg obtained. In the embodiment 5:1 - butyl - 3 - methyl 2 using disaccharide (sucrose) daily allowance ear roots with anti-solvent [...][...] selective crystallization separation The same method in the embodiment 1 embodiment is coated with, a non-daily allowance as well as per the truth returning home pack ear. Disaccharide (sucrose) length 0. 5 Mm or more single crystalline 16. A 8 mg obtained. In the embodiment 6: disaccharide (sucrose) single crystal X - ray ray crystallography In precipitation in the embodiment 1 to 3 is 0. (Sucrose) having a length of at least 5 mm x - ray crystallographic analysis has his embodiment determining the instant. The abstract, all measurements are graphite monochromatic Mo a-Kα radiation (λ=0. 71069 Å) Siemens SMART diffraction meter (Siemens Industrial Automation, Inc, Madison, WI) having using his embodiment. The determination of the Paratone NTM hydrocarbon oil onto the fibers of the glass by using coil springs is blocked, the reading obtained in - 135 ± 1 °C. Range arbitrarily corresponding space structure is calculated per structure 10 S. 0 per structure. Of 3° w scan was collected. In the embodiment 1 to 3 (unit cell) is used disaccharide (sucrose) give US a change in ionic liquid [...] 3c both 24.4 (monoclinic system) alkylation, a seed crystal is (Å)=7. 751, B=8 (Å). 775, (Å) c=10. 804, Determining angle α=γ=90 °, β=103° at both his. The P2 and Space group1 Sweet taste. The crystal structure analysis result also 8 has been shown, in the embodiment 1 to 3 ionic liquids in the disaccharide (sucrose) crystal hydrogen binding site selective crystallization precipitation shown to table 1. Specific techniques of the present invention that are directionally more precisely bar portion, the party industry in person with skill in the art traded only to such preferred yale is received, the range of the present invention that are not one number is the ms.. The, substantial range of the present invention defined by appended claim and their equivalent that will. The present invention relates to a method for separating sucrose by selective crystallization, in which it was confirmed that it is possible to selectively crystallize and separate the sucrose, a pure disaccharide, through drowning-out crystallization by dissolving Angelica gigas in an organic solvent and using an ionic liquid as an anti-solvent. COPYRIGHT KIPO 2018 Sucrose (sucrose) then a selective crystallization separation method including: (a) extracting Angelica gigas Nakai roots with an organic solvent; (b) adding said at least one ionic liquid on the resultant structure of (a); (b) and (c) said resulting induces crystallization of sucrose (sucrose) to obtain crystals. According to Claim 1, said satellite receiver of Korean Angelica gigas Nakai ( According to Claim 1, (a) said step of 5 - 150% (weight/volume) Angelica gigas Nakai roots organic solvents characterized in the method. According to Claim 1, said step (a) of the organic solvent is methanol, ethanol, butanol, isopropyl alcohol, pentane, hexane, heptane, cyclohexane, toluene, acetone, methyl acetate, methylene chloride, chloroform, ether, petroleum ether, benzene, ethylene glycol, propylene glycol and butylene glycol organic solvent is selected from the group consisting of a method characterized. According to Claim 1, said step (a) in the range from 1 to 5 hours embodiment 30 35 °C temperature extraction of the agitating method characterized. According to Claim 1, step (b) said step (a) organic solvents and said at least one ionic liquid volume ratio 0. 2 - 3:1 Characterized in the method. According to Claim 1, said step (b) at least one ionic liquid cationic configuration may be an ammonium cation, imidazole bud cation, oxa the bud which will doze cation, d [nyum piperidinyl cation, it falls the bud antagonist cation, the bud which will doze antagonist cation, it hardens [nyum pyridyl cation, a pyridinium cation, pyrimido liquid-crystal medium cation, cation d [nyum pyrrole, pyrrole [...] cation, pyrrole bud cation, sulfonium cation, phosphonium cation and triazoles nicotineamid cation and, said step (b) at least one ionic liquid anion constituting anion is ethyl alkylsulfate, tosylate anion, methane sulfone anion, tetra [...] anion, process fluorine phosphate anion, selected from the group consisting fluorine process with the claw egg base this [thu which it pushes into a solid conductive rubber 2 carbonate anion and a method characterized anion. According to Claim 7, said step (b) 1 - ethyl - 3 - methyl ethyl [...] of ionic liquid alkylsulfate, 1 - ethyl - 3 - methyl [...] tetra [...], 1 - ethyl - 3 - methyl [...] tosylate, 1 - ethyl - 3 - methyl [...] methane sulfonate, 1 - ethyl - 3 - methyl [...] free plate, 1 - ethyl - 3 - methyl [...] DC into amide, 1 - butyl - 3 - methyl [...] tetra [...], 1 - butyl - 3 - methyl [...] bis (triple release with neel methyl opinion gun) imide, 1 - butyl - 3 - methyl [...] DC into amide, 1 - butyl - 3 - methyl [...] triflate, 1 - butyl - 3 - methyl [...] methane sulfonate, 1, 3 - methyl - 2 - [...][...] bis (triple release with neel methyl opinion gun) imide, 1, 3 - d it will inform and already will be and the bud which will doze [...] 2, 1 - Butyl - 2, 3 - dimethyl [...] triflate, 1 - butyl - 2, 3 - dimethyl [...] tetra [...], 1 - (2 - hydroxyethyl) - 3 - methyl [...][...] 2, it will be burnt [...][...] 1 - 2 - 3 - methyl, 1 - butyl - 3 - methyl [...] MMP 2, MMP 1 - butyl - 4 - methyl 2 [...], 1 - allyl - 3 - tetra ethyl [...][...], 1 - bis (trifluoro methylsulfonyl) methyl -1 profile [...] imide, tree jade [thil ammonium methyl bis (trifluoro methylsulfonyl) imide, butyl tree methyl ammonium bis (trifluoro methylsulfonyl) imide, tree [...] bis (trifluoro methylsulfonyl) bis (trifluoro methylsulfonyl) imide to selected from the group consisting imide and d ethyl methyl opinion gun [nyum characterized method. According to Claim 1, said step (c) said step (b) induction of crystallization of the method embodiment characterized by left to display the resulting 5 - 48 hours. According to Claim 1, said step (c) of determining sucrose (sucrose) projections are 0. 5 Mm or more monocrystalline method characterized.
