СПОСОБ И УСТРОЙСТВО ДЛЯ ОПРЕДЕЛЕНИЯ ПАРАМЕТРОВ СИСТЕМЫ В ЦЕЛЯХ УМЕНЬШЕНИЯ КОРРОЗИИ В УСТАНОВКЕ ПЕРВИЧНОЙ ОБРАБОТКИ НЕФТИ

Изобретение относится к определению количества различных веществ в жидком образце. В устройстве используется по крайней мере один способ оптического анализа, не зависящий от объема и/или концентрации, для определения одного из следующих свойств: водородного показателя pH, количества хлорида и/или количества железа в образце. Оптическое свойство может быть колориметрическим, флуоресцентным или и тем, и другим, причем оно может являться результатом добавления в образец красителей, комплексообразующих агентов, соединений, вызывающих мутность, и других реагентов, вызывающих оптический эффект. Способ также включает использование электрода BDD для окисления веществ (таких как сульфоксидные соединения), которые в противном случае мешали бы проведению оптического анализа, и/или для промывания образца газом. Так как измерения не зависят от концентрации и объема, их можно проводить непрерывно, быстро, тем самым избегая неудобства начала и завершения технологического процесса, а устройство может многократно ...

Устройства детектирования света с защитной облицовкой и относящиеся к ним способы

Изобретение относится к области измерительной техники и касается устройства детектирования света. Устройство содержит реакционную структуру, образующую множество реакционных углублений для вмещения реакционного раствора и реакционного центра, основу устройства, расположенную под реакционной структурой и содержащую множество датчиков света, схему устройства, электрически соединенную с датчиками света, облицовочный слой, проходящий вокруг каждого световода и расположенный между каждым световодом и схемой устройства и защитный слой, проходящий вокруг каждого световода и расположенный между каждым световодом и облицовочным слоем. Реакционный центр выполнен с возможностью генерирования световых излучений под действием света возбуждения после обработки реакционным раствором. Световоды проходят в основу устройства от вводных областей в направлении соответствующего датчика света. Защитный слой предотвращает взаимодействие между реакционным раствором, проходящим через реакционную структуру и световод ...

СПОСОБ И УСТРОЙСТВО ДЛЯ ОПРЕДЕЛЕНИЯ ПАРАМЕТРОВ СИСТЕМЫ В ЦЕЛЯХ УМЕНЬШЕНИЯ КОРРОЗИИ В УСТАНОВКЕ ПЕРВИЧНОЙ ОБРАБОТКИ НЕФТИ

... 1. Способ измерения по крайней мере одного свойства преимущественно жидкого образца, включающий следующие этапы:добавление по крайней мере одного химического реагента к образцу, причем указанный химический реагент, когда его добавляют к образцу, способен вызывать измеряемый оптический эффект, непосредственно связанный с определяемым свойством,измерение указанного оптического эффекта, ивыведение значения свойства путем сравнения измеренного оптического эффекта с заранее определенными значениями, соответствующими определяемому свойству,удаление сульфоксидных соединений из образца путем добавления к нему промывной смеси, причем указанная промывная смесь содержит 2-10% перуксусную кислоту в равновесии с 10-35% перекисью водорода и воду,причем отношение между измеренным оптическим эффектом и определяемым свойством не зависит от объема жидкого образца и от объема реагента, добавленного к образцу, причем промывная смесь не оказывает воздействие на измеряемый оптический эффект за исключением предотвращения ...

МАРКИРОВКА МАТЕРИАЛА, МАРКИРОВОЧНЫЙ МАТЕРИАЛ И ПРОЦЕСС УСТАНОВЛЕНИЯ ПОДЛИННОСТИ ИЛИ АНАЛИЗА РАЗБАВЛЕНИЯ

СПОСОБ АНАЛИЗА МАТРИЧНОЙ НУКЛЕИНОВОЙ КИСЛОТЫ, СПОСОБ АНАЛИЗА ВЕЩЕСТВА-МИШЕНИ, НАБОР ДЛЯ АНАЛИЗА МАТРИЧНОЙ НУКЛЕИНОВОЙ КИСЛОТЫ ИЛИ ВЕЩЕСТВА-МИШЕНИ И АНАЛИЗАТОР ДЛЯ МАТРИЧНОЙ НУКЛЕИНОВОЙ КИСЛОТЫ ИЛИ ВЕЩЕСТВА-МИШЕНИ

Optisches Verfahren zum Messen der Sauerstoffkonzentration und optischer Sensor zum Messen der Sauerstoffkonzentration

Die Erfindung sieht ein optisches Verfahren zum Messen einer Sauerstoffkonzentration und einen optischen Sensor zum Messen einer Sauerstoffkonzentration vor, wobei eine Licht absorbierende Farbstoffmolekülschicht, deren Absorptionsspektrum sich in Abhängigkeit von der Bindung mit Sauerstoffmolekülen ändert, kombiniert ist mit einer Licht emittierenden Schicht, und die Sauerstoffkonzentration vom Umgebungsfluid gemessen werden kann. Die Licht absorbierende Schicht (4), die in einer Sauerstoff abquenchenden Licht emittierenden Schicht (3) laminiert ist, ist ein Film, der einen Kobalt-Porphyrin-Komplex (CoP) oder ein anderes Licht absorbierendes Farbstoffmolekül (7) aufweist, dessen Absorptionsspektrum sich in Abhängigkeit von der Bindung mit Sauerstoffmolekülen ändert. Wenn der Überdeckungsgrad mit dem Lichtemissionsspektrum oder einem Anregungs-(Absorptions-)Spektrum der Licht emittierenden Schicht (3) sich als Ergebnis einer Veränderung in dem Absorptionsspektrum ändert, das von der Licht ...

Vorrichtung und Verfahren zum Messen des Sauerstoffgehalts bei Schweißprozessen

Die Erfindung betrifft eine Vorrichtung zum Messen des Sauerstoffgehalts bei Schweißprozessen, insbesondere Schutzgasschweißen, wobei die Vorrichtung mindestens ein Sensorelement zum Erfassen des Sauerstoffgehalts einer Schutzatmosphäre aufweist. Mit dem Ziel eine Vorrichtung zur Messung des Sauerstoffgehalts bei Schweißprozessen anzugeben, welche selbst bei sehr hohen Temperaturen des Schweißprozesses stabile Messwerte für den Restsauerstoffgehalt liefert und bereits nach kürzester Zeit einsatzbereit ist, ist vorgesehen, das Sensorelement als optischen Sauerstoffsensor auszubilden.

A microparticle assembly

A method of analysing a sample of biological material that includes modifying fluorescent labels in the sample or the environmental conditions of the sample

A method or apparatus for analysing a sample of biological material that includes fluorescent labels. The apparatus uses a processing means. The method comprises the steps of: a) irradiating the sample with excitation energy; b) detecting fluorescent radiation emitted from the labels in the sample; c) Modifying either of: fluorescent labels in the sample or the environmental conditions of the sample with the apparatus in response to a user input after irradiation step (a) or in response to analysis by the processing means after the step (b). The modification in the step (c) may comprise irradiation of a portion of the sample with an energy beam such that the fluorescence of the labels in that portion is reduced. Steps (a) to (c) may be repeated, whereby the time period of repetition may depend on the analysis by the by the processing means. The modification of step (c) may involve modifying optical properties of photo-switchable labels, or it may include forming an opening in a membrane ...

Detection of explosives through luminescence

Method and device

The presence or concentration of analyte (1, Fig. 1) in liquid sample 2 is determined from quenching of an autofluorescence signal of substrate 4, perhaps in lateral flow device 37. Substrate 4 may be inherently fluorescent, not comprising added fluorophores, and may be porous, formed from fibres of cellulose, nitrocellulose, lignin, collagen, cotton or silk. Analyte may be selectively bound by quenching substance 10, which causes quenching of the substrate autofluorescence signal and which may comprise gold nanoparticles, at conjugate pad 39. Analyte quenching complexes 17 may be selectively bound by immobilised binding agent 8 at test region 7. Autofluorescence may be excited by light from an emitter absorbed in a first wavelength range. Analyte concentration may be determined from quenching of the autofluorescence signal combined with absorbance of the quenching substance, measured by photodetectors.

Optode sensor with integrated reference

Method and device

FLUORESZENZIMMUNOASSAY AUF GRUNDLAGE DER FLUORESZENZLOESCHUNG

OPTISCH CHEMISCHER SENSOR

Method for measuring the oxygen content of the head space gas in a beverage can

Die Erfindung betrifft ein Verfahren zur Messung des Sauerstoffgehalts des Kopfraumgases in einer Getränkedose (6), mit insbesondere gekrümmten Boden (20), - wobei die Getränkedose (6) auf dem Kopf stehend, mit dem Boden (20) entgegen der Schwerkraft angeordnet wird, sodass sich das Kopfraumgas im Bereich des Bodens sammelt, - wobei mit einem auf einem Anstechkopf (1) angeordneten hohl ausgebildeten Piercer (2) eine Entnahmeöffnung in den Boden (20), insbesondere in das Zentrum des Bodens (20), der Getränkedose (6) eingebracht wird, in die ein Entnahmerohr (3) eindringt und wobei die Entnahmeöffnung mittels am Piercer (2) oder dem Anstechkopf (1) angeordneten Dichtelementen luftdicht abgedeckt wird, wobei der Flüssigkeitsspiegel in der Getränkedose (6) über das Entnahmerohr (3) derart abgesenkt wird, dass zwischen dem mit Kopfraumgas gefülltem Kopfraum (4) und der Entnahmeöffnung eine direkte Verbindung besteht, wobei nach Absenken des Flüssigkeitsspiegels das im Kopfraum (4) der Getränkedose ...

Method for measuring the oxygen content of the head space gas in a beverage can

Die Erfindung betrifft ein Verfahren zur Messung des Sauerstoffgehalts des Kopfraumgases in einer Getränkedose (6), mit insbesondere gekrümmten Boden (20), - wobei die Getränkedose (6) auf dem Kopf stehend, mit dem Boden (20) entgegen der Schwerkraft angeordnet wird, sodass sich das Kopfraumgas im Bereich des Bodens sammelt, - wobei mit einem auf einem Anstechkopf (1) angeordneten hohl ausgebildeten Piercer (2) eine Entnahmeöffnung in den Boden (20), insbesondere in das Zentrum des Bodens (20), der Getränkedose (6) eingebracht wird, in die ein Entnahmerohr (3) eindringt und wobei die Entnahmeöffnung mittels am Piercer (2) oder dem Anstechkopf (1) angeordneten Dichtelementen luftdicht abgedeckt wird, wobei der Flüssigkeitsspiegel in der Getränkedose (6) über das Entnahmerohr (3) derart abgesenkt wird, dass zwischen dem mit Kopfraumgas gefülltem Kopfraum (4) und der Entnahmeöffnung eine direkte Verbindung besteht, wobei nach Absenken des Flüssigkeitsspiegels das im Kopfraum (4) der Getränkedose ...

PROCEDURE FOR THE DETERMINATION OF THE CONCENTRATION OF MATERIALS, IN PARTICULAR OF OXYGEN, CONTAINED IN A SUBSTANCE

FLUORESZENZIMMUNOASSAY ON BASIS OF THE FLUORESZENZLOESCHUNG

PROCEDURE FOR THE PROOF OF THE EFFECTS OF BREAKDOWN EFFECTS ON MEASURING DATA

KERN-MANTEL NANO-PARTICLE FOR (F) RET TEST PROCEDURE

OPTISCH CHEMISCHER SENSOR

An agent which can deactivate singlet oxygen is used in an optical sensor.

IN VITRO PHOTOMETRIC PROCEDURE FOR THE DETERMINATION OF A BLOOD GAS PARAMETER IN A BLOOD TEST

OPTISCH-CHEMISCHER SENSOR

PHOTOMETRIC PROCEDURE AND CONTAINER FOR IN VITRO ACCOMPLISHED REGULATION OF A GAS IN A BLOOD TEST

Diagnostic test system and method

A diagnostic test system, including: a diagnostic test assembly and a diagnostic test apparatus to perform a test on a biological or environmental sample; the diagnostic test assembly includes: a sample preparation reservoir to receive the sample into a sample preparation fluid, such that a swab carrying the sample can be used to stir the preparation fluid and to wash the swab; a sample dispensing mechanism for insertion into the sample preparation reservoir; a closure to seal the sample preparation reservoir; at least one diagnostic test reservoir coupled to the sample preparation reservoir; and at least one seal between the sample preparation reservoir and the diagnostic test reservoir to prevent fluid movement between the respective reservoirs; wherein the sample dispensing mechanism is operable to disrupt the seal to allow sample fluid to enter the diagnostic test reservoir from the sample preparation reservoir, and to dispense a predetermined amount of fluid.

Systems and methods to perform assays for detecting or quantifying analytes within samples

Fluorescence-based detection methods and apparatus

Optical carbon dioxide sensor, and associated methods of manufacture and use

Optical imaging probes

The present invention relates to methods of visualising cells especially although not exclusively in vivo using a dye, such as a dendrimer-dye molecule or polybranched- dye molecule which is internalised by the cells and thus permits subsequent visualisation by confocal fluorescence endomicroscopy or other optical detectors. There is also provided internally quenched probes for use in visualising cells especially although not exclusively in vivo by confocal fluorescence endomicroscopy and the use of internally quenched probes in combination with confocal fluorescence endomicroscopy, for visualising cells by virtue of internalisation and dequenching of a probe by the cells. In a particular embodiment the cells are activated neutrophils, such as within the lung of a subject.

FABRICATION OF AN APTAMER BIOSENSOR BASED ON QUANTUM DOTS FOR DETECTION AND ELIMINATION OF SALMONELLA

The invention discloses a preparation method and application of aptamer-quantum dots biosensors for the detection and elimination of Salmonella, which belongs to the technical field of detection and inactivation of food pathogenic bacteria. The biosensor of the invention is to connect the aptamer C of Salmonella labeled by quantum dots on the surface of Fe304 nanoparticles coated with poly dopamine (PDA), the complementary Strand A modified with fluorescein was paired with the aforementioned aptamer C, and the Fe304 nanoparticles connected with aptamer C and strand A were used to detect Salmonella. The detection sensitivity of the biosensor is 10 CFU/100 g, and the detection range is 10-104 CFU/100 g, which has good sensitivity and specificity; the sterilization degree is more than 95%, and the effect is remarkable. Deposition of Modification of olydop amn a chain Carbonquanlamdts Complementary pairing of a chain and C chain of aptamer V\A/________ I\J\A\IStrandA-FAM fluoreci Near infrared ...

Nucleic acid-based detection

MICROENCAPSULATION OF OXYGEN-SENSING PARTICLES

The present invention relates to compositions comprising a core and a hydrophobic coating material surrounding the core. The core comprises at least one oxygen- sensing particle. The present invention also relates to methods of detecting and monitoring oxygen in a sample using the microencapsulated oxygen-sensing particles.

APPARATUS AND METHOD FOR SYSTEM IDENTIFICATION

Methods and apparatus for system identification operate by computing phase and amplitude using linear filters. By digitally processing the linearly filtered signals or data, the phase and amplitude based on measurements of the input and output of a system, are determined.

BIOSENSORS FOR DETECTING MACROMOLECULES AND OTHER ANALYTES

Described are compositions and methods that are useful in the identification and quantification of any polypeptide or macromolecular complex using a set of co-aptamer constructs. Aptamer constructs are constructed that bind to unique epitopes of a polypeptide of macromolecular construct. Those aptamer constructs contain an epitope binding site, a co-aptamer binding site, and a detectable label. In the presence of the cognate polypeptide, analyte- polypeptide complex, or other macromolecular complex, the co-aptamers associate with one another to produce a detectable signal. The co-aptamer constructs may be joined by a linker to produce a bivalent aptamer construct.

ADVANCED MULTIFUNCTIONAL/MULTISPECTRAL BIOSENSOR DEVICES AND METHODS OF USE

... ▓▓▓GB0000079 are advanced multifunctional biochip devices capable of specifically ▓detecting and quantitating multiple biomolecular target compounds, such as ▓polypeptides, polynucleotides, and other intracellular and extracellular ▓biomolecules. In illustrative embodiments, the miniaturized multifunctional ▓biosensor device comprises multiple biological sensing elements, excitation ▓micro-lasers, a sampling waveguide equipped with optical fluorescence ▓detectors, integrated electro-optics, a bio-telemetric radio frequency signal ▓generator, and a plurality of molecular probes, all contained on a single ▓integrated circuit, or "biochip". The biochip is suitable for multi-gene ▓analysis, and multi-peptide detection, as well as simultaneous detection and ▓quantitation of polynucleotide and polypeptide species using a single biochip ▓device. Also disclosed are methods that permit rapid, large-scale, and cost-▓effective production of such biochip devices, as well as their use in the ▓detection ...

MEASUREMENT OF A DYNAMIC SYSTEM

A method for performing measurements includes providing relative movement between a receptacle and a cover over a measurement interval comprising multiple non-overlapping time periods. A distance between the receptacle and the cover changes along a first axis over at least a portion of each time period. An apparatus for performing measurements includes a receptacle comprising a substrate and a plurality of wells within the substrate, and a cover. The cover includes: (1) a device configured to engage with the receptacle, (2) a control subsystem configured to provide relative movement between the receptacle and the cover, and (3) a measurement subsystem.

METHODS AND MATERIALS FOR MERCURY DETECTION AND REMOVAL

SYSTEMS AND DEVICES FOR HIGH-THROUGHPUT SEQUENCING WITH SEMICONDUCTOR-BASED DETECTION

In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas (1204', 1214') with different angles of illumination (1201, 1211) during a sequence of sampling events, including, for a sampling event, illuminating each of the wells (1202, 1212) with off-axis illumination (1200A, 1200B) to produce asymmetrically illuminated well regions in each of the wells (1202, 1212).

SYSTEMS AND METHODS TO PERFORM ASSAYS FOR DETECTING OR QUANTIFYING ANALYTES WITHIN SAMPLES

An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.

METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING

A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber fined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative ...

METHOD AND APPARATUS FOR DETECTING UNDESIRED MEASUREMENT CONDITIONS

The invention relates to a method and apparatus for detecting undesired measurement conditions in a sample con-tainer. The method comprises measuring a fluorescent property of the sample container comprising a sample substrate with im-pregnated blood sample and incubation buffer to which the blood sample is to be eluted, and determining, based on temporal and/ or spectral characteristics of the fluorescent property, whether the fluorescent property is characteristic to a sample container com-prising a sample substrate and incubation buffer under said undesired measurement conditions or to a sample container suitable for optical measurement of analyte contained in the sample. Thus undesired measurement condition can be a floating sample sub-strate or a foreign body in the sample container. By means of the invention, reliability of neonatal screening, for example, can be increased.

SYSTEM AND METHODS FOR DETECTING MULTIPLE OPTICAL SIGNALS

An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.

APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING THERMAL MELT ANALYSES AND AMPLIFICATIONS

The present disclosure provides apparatus, systems, and methods for conducting rapid, accurate, and consistent heated amplifications and/or thermal melt analyses.

FLUORESCENCE ANALYZER FOR LIGNIN

E 5171 A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has a sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures are taken to maximize the emission light intensity which is due to lignin concentration and distinguish it from background and interfering light. The fluorescent light intensity is found to drop off in a predictable manner with increased lignin concentration.

AMPLIFIED FLUORESCENCE EMISSION FOR CHEMICAL TRANSDUCTION

A novel optical chemical sensor for direct and continuous detection of organic species in process streams is described. The sensor is based on the use of surface plasmon resonance to amplify fluorescence emission from chemically selective membranes which can be deposited as Langmuir-Blodgett films on thin metal island films.

Method for exact determination of the concentration of a gas, a vapour or a gas dissolved in a sample and fluorescence measurement device used therefor

A method and a device are described for exact determination of the concentration of a gas, a vapour or a gas dissolved in a sample, changes in temperature and in the intensity of the light source being compensated for. The device comprises a sensor element (S) which contains a fluorescence reagent and is coupled to transfer optics such as an optical waveguide (10), a prism or glass tubes, and a reference sensor (R) which contains the same fluorescence reagent. The reference sensor is encapsulated in an air-containing environment. The light emerging from the first sensor and that emerging from the reference sensor is automatically detected and recorded and the concentration of the gas is calculated on the basis of the output signals of the photodetectors (3, 5) by using calibration data of the fluorescence reagent present in the sensors. The reagent is immobilised in the sensor using an adhesive such as, for example, a silicone adhesive. ...

Zusammensetzungen und Verfahren zum Nachweis der Nukleinsäure von Gruppe-B-Streptococcus.

Offenbart sind Nukleinsäureoligomere, einschließlich Amplifikationsoligomere und Nachweissonden, zum Nachweis der Nukleinsäure von Gruppe-B-Streptococcus (GBS; Streptococcus agalactiae). Ebenfalls offenbart werden Verfahren zur spezifischen Nukleinsäure-Amplifikation und zum Nachweis unter Verwendung der offenbarten Oligomere sowie entsprechender Reaktionsgemische und Kits.

MOLECULES FOR SELECTIVE DELIVERY AND METHODS OF THEIR DELIVERY

Tessellated zipper pattern of identically shaped sensor elements and method of manufacture

A sheet of sensors and a method of manufacturing such sheet of sensors. The sheet is configured with (A) a tessellated zipper pattern 20 of identically shaped elements 30 defining (i) a right longitudinal column 21r consisting of a base portion 31 of a right set of elements 30r, (ii) a left longitudinal column 21s consisting of a base portion 31 of a left set of elements 30s, and (iii) an intermediate longitudinal column 21i consisting of alternating tab portions 32 of the right 30r and left 30s elements, and (B) a continuous longitudinal strip of functional material 70 positioned only within the intermediate column 21i.

Low-cost composite fluorescent nano-fiber thin film with high selectivity for detecting TNT as well as preparation method and application thereof

Kit for one-step simultaneous detection of exchangeable copper and ceruloplasmin in serum, preparation method of kit and application of kit

Method for preparing carbon quantum dot by strong alkali cutting of graphite oxide, and product and application thereof

Ochratoxin A (OTA) fluorescence detection method adopting carbon nitride nanosheet and aptamer coupled for sensing

Template agent-oriented-synthesized porous coordination polymer as well as preparation and application thereof

The invention relates to a template-agent-oriented-synthesized porous coordination polymer as well as a preparation method and application thereof. The coordination polymer has the chemical formula of {Cd3(cia)2(4,4'-bipy).2H2O}n; wherein cia is an anion of 2-(4-carboxyphenoxy)terephthalic acid after deprotonation, 4,4'-bipy is 4,4'-bipyridine organic ligand, n is the degree of polymerization. According to the invention, 1,4-bis(1,2,4-triazolyl-1-methylene)benzene is used as a hydrothermal reaction template agent for the first time, and by controlling the reaction conditions, a porous coordination polymer with porosity of 31.6% is prepared; the coordination polymer has good thermal stability, high fluorescence intensity, clear blue light and pure color, and can be used as a fluorescent probe to efficiently detect nitrobenzene object organic molecules. The preparation method has the advantages of simple preparation process, high crystal purity, high yield, simple detection method, high selectivity ...

용존 산소 센서의 컴포넌트 수명 종료의 검출 및 시그널링을 위한 시스템 및 방법

... 여기에 개시된 실시형태는 유체 유동 경로에 노출된 형광체를 포함하는 센서를 포함할 수 있다. 형광체는 여자 광원에 의한 조명에 응답하여 광을 방출할 수 있다. 조명에 응답하여 형광체에 의해 방출된 광의 크기가 결정될 수 있다. 이 크기가 기준 크기의 임계치 내인지 여부가 결정될 수 있고 이 결정에 기초하여 알람 상태가 설정된다. 이 알람 상태는, 형광체가 수명 종료 상태에 도달하였거나 아니면 교체되어야 함을 나타낼 수 있다.

A METHOD OF ANALYSING A SAMPLE AND APPARATUS THEREFOR

A method of analysing a sample of biological material using apparatus including processing means comprises the steps of: (a) irradiating the sample with electromagnetic and/or ionizing radiation; (b) detecting electromagnetic radiation emanating from the sample and generating a signal in response thereto; and (c) modifying the sample, labels in the sample and/or the environmental conditions of the sample with the apparatus in response to user input after the beginning of step (a) and/or in response to analysis by the processing means of the signal generated in radiation detection step (b). Thus modifications may be determined and executed interactively as an experiment progresses, and therefore directed towards specific structures and/or events identified as of particular interest in the course of the experiment. © KIPO & WIPO 2007 ...

MARKING OF MATERIAL, MARKED MATERIAL AND PROCESS OF AUTHENTICATION OR DILUTION DETERMINATION

Method for marking a material, comprising including at least two components having different fluorescent characteristics as a blend of components in the material, the at least two components not being already associated with the material and at least one of the at least two different components having a fluorescence that varies in spectral position and/or intensity according to variation of pH, the at least two components being included in the material in an amount effective to be qualitatively and/or quantitatively determined. Also, provided are marked materials and methods of authenticating and preventing counterfeiting and dilution.

ONLINE CONTAMINANT DETECTION AND REMOVAL SYSTEM

This invention refers to continuous flow devices for detecting and/or removal of contaminants from a liquid stream. The device comprises a cartridge with an inlet and outlet for the liquid stream, a radiation incident and emerging wall portion and optically transparent support material packed in the cartridge such that a liquid stream can pass between voids or spaces formed within the optically transparent material, as well as a radiation source and detector. The support material comprises molecules for capturing at least one contaminant on the surface of the support material and each of the capture molecules comprises at least one reporter group which emits a signal upon binding of the contaminant. Another form of the invention refers to a rotatable support within the liquid stream.

DEVICE FOR MEASURING OXYGEN IN FLUIDS

The device has an excitation signal generator with a block (2) for optoelectronically detecting fluorescent radiation and electronically amplifying the detected fluorescence signal, with a filtering block, with a block (4) for modulating the amplitude of the radiation arriving at the fluorescence sensor, and with an electronic phase detector block (5) connected to a microcontroller (6); with the known method for evaluating the phase shift between the phase of the signal which excites a fluorescence sensor inserted into the fluid and the phase of the emitted fluorescence signal being used in the measurement. Said filtering block is a multiple filter (3) with switchable capacitances, while said excitation signal generator is a generator (1) from which at least one common clock signal is extracted in order to synchronize the sections of said multiple filter.

MICROWELL ARRAYS FOR DIRECT QUANTIFICATION OF ANALYTES ON A FLAT SAMPLE

The present invention relates to a bioanalytical device consisting of a microwell array with microwell (2) that are filled with assay components (12, 15, 36), wherein detection probes (36) used in the assay (10) are metal nanoparticles (1 1, 12) or fluorescent compounds, and wherein the microwell array is connected and/or connectable to a sample that is on a flat substrate (6) to quantify the amount of a ligand (35) in the sample by using a detection mechanism. The detection mechanism is based on change in the optical properties of some of the assay components (12, 15, 36) upon contact with the ligand (35). The present invention also relates further to a method for detecting and quantifying molecules using said bioanalytical device.

SYSTEMS AND METHODS FOR ENHANCING FLUORESCENT DETECTION OF TARGET MOLECULES IN A TEST SAMPLE

Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample.

QUANTUM DOT-SENSORY ARRAY FOR BIOLOGICAL RECOGNITION

The present invention provides a quantum dot-based biomolecule sensor array capable of differentiating the strain of a variety of biological molecules including bacteria, spores, fungi, viruses, and disease-causing prions. The biosensor uses specific chemical functionalities that regulate the interactions between different chemical ligands and biological molecules.

METHODS, SYSTEMS AND APPARATUS FOR LIGHT CONCENTRATING MECHANISMS

An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.

Receptacles for storing substances in different physical states

A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal ...

Multicolor microwave-accelerated metal-enhanced fluorescence (M-MAMEF)

The present invention relates to the use of multiple different light emitting molecules that emit different and detectable emission signals to provide systems and methods to detect different target products in a single assay sample, wherein the different light emitting molecules are positioned an optimal distance from metallic particles thereby enhancing emissions. Preferably, the systems and methods further comprise use of either microwave or sonic energy to increase binding reactions, timing of such reactions within the assay sample and reduce background non-specific biological absorption.

Sensors for measuring analytes

A sensor and system using the sensor for detecting an analyte, where the sensor includes an amorphous fluorinated polymer and a luminescent metal-ligand complex is provided. Sensor systems for monitoring oxygen in environments containing volatile organic solvents are also provided.

Fluorescent polymer-QTL approach to biosensing

A chemical composition including a moiety comprising a quencher (Q), a tethering element (T), and a ligand (L) that associates with and quenches a fluorescent polymer is disclosed. When an analyte sample is introduced, the ligand (L) binds to a target biological agent if it is present, thereby causing the QTL molecule to separate from the fluorescent polymer resulting in an increase in detected fluorescence. The same chemistry is advantageously employed in a competitive assay. An electric field can also be applied to separate the QTL molecule from the fluorescent polymer. A method for detecting targets for these methods are also disclosed.

Selective delivery molecules and methods of use

Disclosed herein is a selective delivery molecule comprising: (a) an acidic sequence (portion A) which is effective to inhibit or prevent the uptake into cells or tissue retention, (b) a molecular transport or retention sequence (portion B), and (c) a linker between portion A and portion B, and (d) at least one cargo moiety.

Apparatus and methods for time-resolved optical spectroscopy

Frequency-domain light detection systems and components and uses thereof for performing time-resolved luminescence assays. The systems may include methods for identifying and/or correcting for background and/or quenching, among others. The systems also may include apparatus for increasing duty cycle and/or sensitivity, among others.

Synthesis and composition of amino acid linking groups conjugated to compounds used for the targeted imaging of tumors

The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a pteroyl ligand that binds to a target receptor protein, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid or derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

CO2 Quantitative Fluorescent Sensing Material, Preparation Method and Application thereof

The present disclosure discloses a CO2 quantitative fluorescent sensing material, a preparation method and an application thereof. The preparation method for the CO2 quantitative fluorescent sensing material includes dissolving 9,10-diacrylic anthracene in a solvent to prepare 5-10 mg/mL of a first solution; dissolving MnCl2 or Mn(ClO4)2 in water to prepare 50-100 mg/mL of a second solution; mixing the first solution and the second solution; adding a diluted acid into the mixed solution; sealing and heating the mixed solution. This preparation method is simple. During application, an ionic liquid produced by a reaction of CO2 gas and an amine compound improves an aggregation-induced emission of the CO2 quantitative fluorescent sensing material and a fluorescence thereof is significantly improved. So that a fluorescent CO2 quantification is performed rapidly and accurately.

Library of pH responsive polymers and nanoprobes thereof

The present disclosure relates to polymers which contain a hydrophobic and hydrophilic segment which is sensitive to pH. In some aspects, the polymers form a micelle which is sensitive to pH and results in a change in fluorescence based upon the particular pH. In some aspects, the disclosure also provides methods of using the polymers for the imaging of cellular or extracellular environment or delivering a drug.

Three-component biosensors for detecting macromolecules and other analytes

The invention generally provides three-component molecular biosensors. The molecular biosensors are useful in several methods including in the identification and quantification of target molecules.

Fluorescent Polymer Dots, Methods For Their Preparation, And Uses Thereof

There is provided a fluorescent polymer dot (FPdot) comprising one or more fluorescent conjugated polymers forming a hydrophobic core and one or more amphiphilic molecules, each amphiphilic molecule comprising a hydrophobic end embedded in the core and a hydrophilic end that forms a hydrophilic shell surrounding the core, wherein said hydrophobic end comprises one or more aliphatic chain moieties. There is also provided a method for preparing the fluorescent polymer dot comprising preparing a mixture of conjugated polymer and amphiphilic molecule in an aprotic solvent; and adding said mixture to a protic solvent. The fluorescent polymer dots may be useful in biomedical imaging applications.

METHOD FOR ANALYZING TEMPLATE NUCLEIC ACID, METHOD FOR ANALYZING TARGET SUBSTANCE, ANALYSIS KIT FOR TEMPLATE NUCLEIC ACID OR TARGET SUBSTANCE, AND ANALYZER FOR TEMPLATE NUCLEIC ACID OR TARGET SUBSTANCE

The present invention provides a method for analyzing a template nucleic acid, a method for analyzing a target substance, an analysis kit for a template nucleic acid or a target substance, and an analyzer for a template nucleic acid or a target substance, which are excellent in accuracy. The method for analyzing a template nucleic acid of the present invention includes the steps of: fractionating a sample containing a template nucleic acid into a plurality of template nucleic acid fractions; amplifying a target sequence and its complementary sequence in the template nucleic acid with respect to each of the plurality of template nucleic acid fractions in the presence of a nucleic acid amplification reagent; detecting generation or quenching of a signal that shows an amplification of the target sequence or the complementary sequence with respect to each of the plurality of template nucleic acid fractions after the amplification step; and discriminating a template nucleic acid fraction in which the generation or quenching of a signal that shows the amplification has been detected among the plurality of template nucleic acid fractions as an amplified fraction in which the target sequence or the complementary sequence has been amplified, wherein the nucleic acid amplification reagent contains a primer set that amplifies the target sequence and the complementary sequence and a signal generating substance that generates or quenches a signal in response to the amplification, and the signal generating substance generates a signal in a state where it is bound sequence-dependently and quenches a signal in a state where it is not bound or quenches a signal in a state where it is bound sequence-dependently and generates a signal in a state where it is not bound, and generation and quenching of a signal are reversible. 1. A method for analyzing a template nucleic acid , comprising the steps of:fractionating a sample comprising a template nucleic acid into a plurality of template ...

High throughput measurement of DNA base lesion repair capacity

The invention pertains to methods for measuring DNA repair capacity of a test solution, for example, a cell lysate. The method can comprise incubating the test solution with a damaged DNA molecule and measuring the damaged DNA remaining at the end of the incubation. The damaged DNA used in the method comprises a marker attached to a first strand of the damaged DNA and a quencher attached to a second strand of the damaged DNA, characterized in that the quencher inhibits the release of a detectable signal from the marker when damaged DNA is in a double stranded form and the marker emits the detectable signal when at least a portion of the damaged DNA is in a single stranded form. The invention also provides screening assays for identifying one or more compounds from a plurality of compounds as an inhibitor of DNA repair mechanism.

Optical-chemical Sensor

An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.

Apparatus for quantitative analysis of a nucleic acid amplification reaction

An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. The apparatus also includes a controller in communication with the detection mechanism. The controller is programmed to perform the steps of deriving a growth curve from the measurements of the signal; calculating a derivative of the growth curve; identifying a characteristic of the derivative; and determining a threshold value associated with the characteristic of the derivative. Embodiments of an apparatus for determining a starting quantity of a nucleic acid sequence in a test sample are also provided.

Oxygen monitoring apparatus and methods of using the apparatus

Apparatus or systems which employ luminescence quenching to produce an oxygen concentration indicative signal. Components of such systems include: (1) an airway adapter, sampling cell, or the like having a casing and a sensor which is excited into luminescence with the luminescence decaying in a manner reflecting the concentration of oxygen in gases flowing through the airway adapter or other flow device and is in intimate contact with a window in the casing; (2) a transducer with has a light source for exciting a luminescable composition in the sensor into luminescence, a light sensitive detector for converting energy emitted from the luminescing composition as that composition is quenched into an electrical signal indicative of oxygen concentration in the gases being monitored, and a casing which locates the light source and detector in close physical proximity to the window but on the side thereof opposite the sensor; and (3) subsystems for maintaining the sensor temperature constant ...

FLUORESCENCE GAS AND LIQUID SENSOR

An optical sensor for the detecting the presence of a gas or liquid comprising: a light source, a substrate, an active layer configured to emit light when illuminated by the optical light source and a detector; wherein the substrate and light source are arranged such that the majority or all of the light from the light source is reflected and/or refracted away from the detector and the detector is arranged to receive part of the light emitted by the active layer.

Bromate ion measurement method

A method for measuring bromate ion is provided that provides high-sensitivity measurement results more simply and more quickly than conventional bromate ion measurement methods. A fluorescent substance that is quenched by coexistence with bromate ions is added to a sample 130 and the fluorescence intensity of the fluorescent substance after quenching is measured, the measured fluorescence intensity being subtracted from the fluorescence intensity of a standard sample containing no bromate ions to calculated the fluorescence intensity difference. The bromate ion concentration is calculated from the calculated fluorescence intensity difference, using a pre-determined calibration line between the fluorescence intensity difference and the bromate ion concentration.

CONVERTER FOR USE WITH SENSING DEVICES

A system and method are disclosed for utilizing sensors with existing devices. An interface module is used in combination with a newer sensor, such as a fluorescence oxygen sensor, and an older legacy device. The older legacy device supplies a polarizing voltage, and anticipates a measured current of between 0 and 100 nA. The newer sensor requires no polarizing voltage and delivers an output of 0-10 volts in one embodiment, and 4-20 mA in another embodiment. The interface module receives the output from the sensor, and converts it into a useable signal to the legacy device. In another embodiment, the interface module comprises a number of outputs, such that both legacy devices and newer devices can be in communication with the sensor simultaneously. The interface module can be used in conjunction with a reactor chamber or other pharmaceutical process.

Temporal sensing and related methods

Embodiments described herein generally relate to: sensing and/or authentication using luminescence imaging; diagnostic assays, systems, and related methods; temporal thermal sensing and related methods; and/or to emissive species, such as those excitable by white light, and related systems and methods.

METHOD OF DETECTION

A method for detection of a nerve agent in a sample, which method comprises (a) irradiating an optical sensing element, the optical sensing element comprising a fluorescent sensing compound provided on a substrate, and measuring the luminescence of the optical sensing element; wherein the fluorescent sensing compound comprises a combination of at least one electron acceptor moiety and, optionally, one or more electron donor moieties such that the electronic properties of the sensing compound are sufficient to enable a change in the luminescence of the sensing element in the presence of the nerve agent, and a moiety that influences solubility of the sensing compound in a solvent; (b) contacting the sample with the optical sensing element; (c) measuring the luminescence of the optical sensing element; and (d) determining whether the nerve agent is present in the sample based on the measurements obtained in steps (a) and (c).

IN VITRO METHOD OF DETERMINING CHANGES IN A PROTEIN ENVIRONMENT

APPARATUS AND METHOD FOR SYSTEM IDENTIFICATION

Method for evaluating analyte

In an analyte evaluation method for evaluating an analyte, AC voltage is applied between a substrate electrode (2) on a substrate (4) and a counter electrode (8), and signals obtained from a marker (3) provided on an analyte bound to the substrate electrode are observed, wherein the frequency of the AC voltage is changed and the behavior of the average value of the marker signals is observed. A novel, highly-selective, low-noise method of evaluating a object of evaluation is thus achieved.

Method and device for rapidly ascertaining the parameters of a sample medium

Light of a defined wavelength is directed towards a luminescent layer which comes into contact with the sample medium and the luminescent properties of which depend on the parameter to be determined. The luminescent light is determined via detectors, the signals of which are a measure of the parameter. So that a reliable determination of a parameter becomes possible with an extremely short adjustment time even in the presence of several parameters influencing the luminescent properties, it is provided to determine the intensity of luminescence for a number of different wavelength bands corresponding to the number of parameters, in which arrangement the parameters have a different influence on the properties of luminescence in at least one wavelength band. The signals obtained are supplied to a signal processing device (26) which determines from these the magnitude of the parameter to be measured. ...

Method and device for rapidly ascertaining the parameters of a sample medium

МАРКИРОВКА МАТЕРИАЛА, МАРКИРОВОЧНЫЙ МАТЕРИАЛ И ПРОЦЕСС УСТАНОВЛЕНИЯ ПОДЛИННОСТИ ИЛИ АНАЛИЗА РАЗБАВЛЕНИЯ

Группа изобретений относится к маркировке товаров. Способ маркировки материала включает в себя добавление смеси компонентов, имеющих различные характеристики флуоресценции, в указанный материал, где смесь компонентов до этого не ассоциировались с данным материалом, и где по меньшей мере один из компонентов смеси характеризуется флуоресценцией, изменяющейся по спектральному положению и/или интенсивности при изменении рН, причем смесь компонентов включена в материал в количестве, достаточном для ее качественного и/или количественного определения. Способ определения подлинности материала посредством определения присутствия смеси компонентов, изменяющих спектральное положение и/или интенсивность при изменении рН, причем указанная смесь компонентов добавлена в материал в качестве маркера, и компоненты смеси не ассоциировались с указанным материалом. Способ подтверждения того, подвергался ли материал изменению или фальсификации путем определения концентрации двух или более компонентов смеси компонентов ...

СЕКВЕНИРОВАНИЕ С ВЫСОКОЙ ПРОПУСКНОЙ СПОСОБНОСТЬЮ С ДЕТЕКТИРОВАНИЕМ НА ОСНОВЕ ПОЛУПРОВОДНИКА

Изобретение относится к секвенированию с детектированием на основе КМОП и, более конкретно, к системам и способам для повышения пропускной способности секвенирования с помощью детектирования на основе КМОП. Биодатчик для определения оснований в последовательности нуклеиновой кислоты содержит устройство анализа образца, которое содержит поверхность для образцов, имеющую массив областей пикселей, и твердотельный формирователь изображения, имеющий матрицу датчиков, причем каждый датчик формирует сигналы пикселей в каждом цикле определения оснований, каждый сигнал пикселя представляет свет, получаемый в одном цикле определения оснований из одного или более кластеров в соответствующей области пикселя поверхности для образцов; и процессор сигналов, выполненный с возможностью соединения с устройством анализа образцов, которое принимает и обрабатывает сигналы пикселей с датчиков для определения оснований в цикле определения оснований и использует сигналы пикселей из меньшего числа датчиков, чем ...

ГЕНЕРАЛИЗОВАННОЕ СТОХАСТИЧЕСКОЕ СЕКВЕНИРОВАНИЕ СВЕРХРАЗРЕШЕНИЯ

Группа изобретений относится к области биотехнологии. Предложен способ секвенирования полинуклеотидов и система визуализации для осуществления указанного способа. Способ включает присоединение первой и второй молекул ДНК-матрицы к первому и второму элементам для присоединения, соответственно, обеспечение включающей набор нуклеотидов химии стохастического фотопереключения, визуализацию серии событий включения и выключения для первой и второй молекул ДНК-матрицы в реальном времени. Система содержит контейнер для образца с множеством элементов для присоединения молекул ДНК-матрицы и визуализатор для визуализации происходящего на первом и втором элементах для присоединения фотопереключения. Причем серия событий включения и выключения для первой и второй молекул ДНК-матрицы являются стохастическими и не синхронизированными, а расстояние между первым элементом для присоединения и вторым элементом для присоединения меньше, чем предел Аббе. Изобретения обеспечивают одновременную реакцию для всех ...

СПОСОБЫ И СИСТЕМЫ ДЛЯ НЕРАЗРУШАЮЩЕГО КОНТРОЛЯ

OPTISCHER CO2 SENSOR UND VERFAHREN ZU DESSEN HERSTELLUNG UND ANWENDUNG

IN VITRO PHOTOMETRISCHES VERFAHREN ZUR BESTIMMUNG EINES BLUTGASPARAMETERS IN EINER BLUTPROBE

Vorrichtung zur Reduzierung der lntensitätsminderung des Fluoreszenzfarbstoffes durch Laserlicht bei der Bestimmung der Fluoreszenz und der Anzahl von Antikörpern auf Exosomen

Vorrichtung zur Reduzierung der Qualitätsminderung des Fluoreszenzfarbstoffes mittels Laserlicht bei der Bestimmung der Fluoreszenz und der Anzahl von Antikörpern auf Exosomen mit den folgenden Merkmalen:a) Mitteln zur Speicherung von verschiedenen Messpunkten 26 unterschiedlicher verschiedenfarbiger Laser in einer Messzelle (17) an bestimmten Messpositionen, wobei die Fokussierung des jeweiligen Laserstrahls (24) in Wechselwirkung mit der Probe (18) als Zentrum eines konvergenten Strahlenbündels in einer Viideokamera registriert wird,b) Eine Wechselvorrichtng 7, für das Notchfilter 6 und einem Quarzglas 16 zur Einstellung und Sicherung der gleichen optischen Weglängen für das Floureszenslicht und das Laserstreulicht im konvergenten Strahlengang zwischen der Flüssigkeits - Linse (15 ) und den Messorten 26 in der Messzelle (17),c) ein Display (9) mit einem Touchscrean und eine Gesamt - Steuerung (10 ) mit einem Partikel - Tracking - Programm dienen der Bedienung einer Videokamera (13), ...

ZUSAMMENSETZUNGEN UND VERFAHREN ZUM NACHWEIS DER NUKLEINSÄURE VON GRUPPE-B-STREPTOCOCCUS

Zusammensetzung zur Bestimmung der Anwesenheit oder Abwesenheit von Gruppe-B-Streptococcus (GBS) in einer Probe, wobei die Zusammensetzung umfasst:eine erste Amplifikationsoligomeren-Kombination und/oder eine zweite Amplifikationsoligomeren-Kombination, wobei(I) die erste Amplifikationsoligomeren-Kombination erste und zweite SIP-spezifische Amplifikationsoligomere umfasst, die in der Lage sind, eine Zielregion einer GBS-SIP-Zielnukleinsäure zu amplifizieren, wobei die ersten und zweiten SIP-spezifischen Amplifikationsoligomere jeweils erste (A) und zweite (B) SIP-spezifische Zielhybridisierungssequenzen umfassen, ausgewählt aus der Gruppe bestehend aus(a)(A) SEQ ID NO:3 oder einem RNA-Äquivalent oder einer DNA/RNA-Chimäre davon und(B) SEQ ID NO:4 oder einem RNA-Äquivalent oder einer DNA/RNA-Chimäre davon; und(b)(A) SEQ ID NO:7 oder einem RNA-Äquivalent oder einer DNA/RNA-Chimäre davon und(B) SEQ ID NO:8 oder einem RNA-Äquivalent oder einer DNA/RNA-Chimäre davon; und(II) die zweite Amplifikationsoligomeren-Kombination ...

Measuring apparatus and method for biological samples

A measuring apparatus 100 for analyzing one or more biological samples includes: an optical source 110 for generating interrogating radiation; a sample arrangement 130 for maintaining samples in a spatial position for the radiation to interrogate the samples; an optical detection arrangement 150 for receiving radiation generated by the samples operable to generate one or more detection signals 160; and a data processing arrangement 170 for processing the detection signals to provide an output 180 indicative of one or more substances and/or pathogens present in the samples. The apparatus operates to perform at least one of: interrogating the samples with plural radiation wavelengths; receiving fluorescent radiation generated by the samples and distinguishing components of the radiation on account of wavelength and/or optical temporal decay characteristics; receiving radiation generated by the samples and distinguishing components of the radiation on account of wavelength; receiving radiation ...

Oxygen Concentration Sensors and Methods of Rapidly Measuring the Concentration of Oxygen in Fluids

Provided are sensors and methods of measuring the oxygen concentration of a fluid. An excitation light source is in optical communication with a transducer for transmitting an excitation light that is at least partially absorbed by the transducer. The transducer has a property of photoluminescence, and enters a higher energy state by at least partially absorbing the excitation light and enters a lower energy state through radiation of emitted light, thus producing spectral indicia. A light detection system, which is also in optical communication with the transducer, processes the spectral indicia to determine the concentration of oxygen in the fluid.

Optical probe containing oxygen, temperature, and pressure sensors and monitoring and control systems containing the same

A probe for measuring oxygen, temperature, and pressure having a housing, made of a thermally conductive material; an oxygen sensor within the housing, a temperature sensor disposed within the housing adjacent to the thermally conductive material, comprising a fiber Bragg grating, a pressure sensor disposed within the housing, comprising a fiber Bragg grating.

Photoluminescent oxygen probe with reduced cross-sensitivity to humidity

An oxygen-sensitive luminescent element, and probe constructed therefrom, having reduced cross-sensitivity to humidity, and methods of manufacturing and using such luminescent elements and probes to measure oxygen concentrations within an enclosed space. The luminescent element includes a glass fiber carrier substrate bearing an oxygen-sensitive photoluminescent dye. The dye is preferably embedded within an oxygen-permeable hydrophobic polymer matrix. A probe is constructed from the luminescent element by laminating the luminescent element onto a structural support layer.

Fluorescence quenching based oxygen sensor

A fluorescence quenching based oxygen sensor can be prepared comprising a polystyrene polymer linked to pyrene. The fluorescence based sensor has the formula (I), Polystyrene-Y—R-Pyrene  (I); wherein Y is fluorescence quenching and R is an aliphatic linking group having 1 to 11 carbon atoms. The sensor can be coated onto a support and integrated with an LED excitation source and fluorescence detector.

Nanohybrid nitrogen monoxide detecting sensor and a production method therefor

The present invention provides a nanohybrid type nitrogen monoxide detecting sensor and a production method therefor in which the nanohybrid type nitrogen monoxide detecting sensor includes a fluorescent semiconducting quantum dot and a transition metal compound. Employing a nanohybrid structure including semiconducting quantum dot nano-particles combined with a molecule recognizer selectively looming a bonding to nitrogen monoxide, the nitrogen monoxide detecting sensor is enabled to detect an infinitesimal amount of nitrogen monoxide by bringing about photoluminescence upon detection of nitrogen monoxide.

Explosives Detection Substrate and Methods of Using the Same

Provided herein are explosives detection substrates which include an electrospun (electro)sprayed and/or dry spun aromatic polymer, such as polystyrene, and a small molecule fluorophore. Methods for detecting an explosive material using such substrates are also provided.

Bromate ion measurement method

A method for measuring bromate ion is provided that provides high-sensitivity measurement results more simply and more quickly than conventional bromate ion measurement methods. A fluorescent substance that is quenched by coexistence with bromate ions is added to a sample 130 and the fluorescence intensity of the fluorescent substance after quenching is measured, the measured fluorescence intensity being subtracted from the fluorescence intensity of a standard sample containing no bromate ions to calculated the fluorescence intensity difference. The bromate ion concentration is calculated from the calculated fluorescence intensity difference, using a pre-determined calibration line between the fluorescence intensity difference and the bromate ion concentration.

Device and method for detecting biomolecule

Disclosed are a method for detecting a biomolecule including: immobilizing a nucleic acid aptamer capable of specifically binding to a biomolecule to be detected on the surface of a bead on which fluorophores are arranged; hybridizing the nucleic acid aptamer with a guard nucleic acid (g-nucleic acid) labeled with a quencher to quench fluorescence; and reacting a sample including the biomolecule to be detected with the nucleic acid aptamer and detecting a fluorescence signal emitted as the biomolecule binds with the nucleic acid aptamer and the g-nucleic acid labeled with the quencher is separated, and a device for detecting a biomolecule for conducting the detection method. The present disclosure allows for effective, convenient and fast detection of the biomolecule to be detected, enables quantitative analysis, and enables detection of even a trace amount of sample.

Preparation of microfluidic device on metal nanoparticle coated surface, and use thereof for nucleic acid detection

The invention relates to a microfluidic device that utilizes nucleic acid-based detection and a detection system containing the same, as well as a process for preparing the micro fluidic device and for using the same to detect the presence of a target nucleic acid molecule in a sample.

Kit for quantitative detection of braf mutation

The present invention relates to a method and assay kit for BRAF gene mutations which relates to the effect of molecule-targeting anti-tumor drug. Particularly, the present invention relates to a fluorescent quantitative PCR method and kit for detecting mutations at hotspots of BRAF gene, together with the use thereof. The present invention detects the mutations at specific sites of BRAF gene, and can predict the therapeutic efficacy of anti-EGFR tyrosine kinase inhibitors, an anti-tumor drug. Therefore, the present invention can provide a guidance to individualized treatments for cancer patients.

Optical probe containing oxygen, temperature, and pressure sensors and monitoring and control systems containing the same

A probe for measuring oxygen, temperature, and pressure in a space to be monitored, comprising: a housing, comprising a thermally conductive material; an oxygen sensor disposed within the housing, comprising: a first end having coated thereon a coating which fluoresces at a fluorescent frequency when exposed to light having an excitation frequency in the absence of associated oxygen, and which undergoes a dampening of said fluorescence in the presence of associated oxygen; and a second end operatively connected to an optical fiber that extends through the housing; wherein the first end extends through the housing and is adapted to be exposed to the space to be monitored; a temperature sensor disposed within the housing adjacent to the thermally conductive material, comprising a fiber Bragg grating, or a semiconductor material, such as a GaAS material, wherein the temperature sensor does not extend through the housing and is not exposed to the space to be monitored; a pressure sensor disposed within the housing, comprising a fiber Bragg grating or a Fabry-Pérot white light interferometry sensor having a first end which extends through the housing and is adapted to be exposed to the space to be monitored.

Polymerase chain reaction detection system

The present invention relates to methods and kits for nucleic acid detection in an assay system.

Nanofibril materials for highly sensitive and selective sensing of amines

A sensory material with high sensitivity, selectivity, and photostability has been developed for vapor probing of organic amines. The sensory material is a perylene-3,4,9,10-tetracarboxyl compound having amine binding groups and the following formula where A and A′ are independently chosen from N—R1, N—R2, and O such that both A and A′ are not O, and R1 through R10 are amine binding moieties, solubility enhancing groups, or hydrogen such that at least one of R1 through R10 is an amine binding moiety. This perylene compound can be formed into well-defined nanofibers. Upon deposition onto a substrate, the entangled nanofibers form a meshlike, highly porous film, which enables expedient diffusion of gaseous analyte molecules within the film matrix, leading to a milliseconds response for vapor sensing.

Method for desulfurizing hot metal

In a method for desulfurizing hot metal by analyzing S concentration of a sample taken out from the hot metal, the S concentration is analyzed rapidly and precisely by a method comprising a high frequency induction heating step of oxidizing the sample under a high frequency induction heating in a pure oxygen atmosphere to convert S in the hot metal to SO 2 and an analysis step of analyzing SO 2 -containing gas generated in the high frequency induction heating step through an ultraviolet fluorescence method to quantify S concentration in the sample, whereby S concentration after the desulfurization is controlled precisely and hence fault of S concentration is prevented but also the increase of the cost due to the excessive addition of a desulfurization agent and step disruption at steel-making step are prevented.

LANTHANIDE CLUSTERS AND METHODS OF USE THEREOF

The present invention is directed to multinuclear lanthanides chiral clusters, based on phenyl-oxazoline-amide (POxA) ligands, and to methods of use thereof. The chiral clusters of this invention are highly fluorescent with high stability. 2. The chiral structure of claim 1 , wherein Ris H claim 1 , halogen claim 1 , —C≡C claim 1 , SOH claim 1 , SONa claim 1 , NHor NO.3. The chiral cluster of claim 1 , wherein Ris an alkyl.4. The chiral cluster of . wherein Ris an alkyl or saturated or unsaturated cycloalkyl or heterocycle.5. (canceled)6. The chiral structure of claim 1 , wherein Ris hydrogen.9. The chiral cluster of claim 7 , wherein said oxygen based ligand is alcohol or water.10. The chiral cluster of claim 1 , wherein said lanthanide is La(III) claim 1 , Pr(III) claim 1 , Pm(III) claim 1 , Sm(III) claim 1 , Eu(III) claim 1 , Gd(III) claim 1 , Tb(III) claim 1 , Dy(III) claim 1 , Ho(III) claim 1 , Er(III) claim 1 , Yb(III) or Lu(III).11. The chiral cluster of claim 1 , wherein said lanthanide is the same or different.12. The chiral cluster of claim 1 , wherein said phenyl-oxazoline-amide (POxA) ligand is a cis isomer with 4R claim 1 ,5R or 4S claim 1 ,5S chiral centers and said cluster is a three lanthanides cluster with six phenyl-oxazoline-amide (POxA) ligands.13. The chiral cluster of claim 1 , wherein said phenyl-oxazoline-amide (POxA) ligand is a trans isomer with 4R claim 1 ,5S or 4S claim 1 , 5R chiral centers and said cluster is a seven lanthanides cluster with nine phenyl-oxazoline-amide (POxA) ligands.14. (canceled)15. (canceled)16. The chiral cluster of claim 1 , wherein said cluster further comprises oxygen bridges between the lanthanides.17. The chiral cluster of claim 1 , wherein said cluster is emitting circularly polarized luminescence (CPL).182334455. A crystalline structure of said chiral cluster of claim 7 , wherein said three lanthanide cluster is represented by the structures of claim 7 , C claim 7 , A claim 7 , B claim 7 , A claim 7 , B claim ...

CROSS-LINKED POLYMER NETWORKS AND METHODS OF MAKING AND USING SAME

Cross-linked polymer networks that are at least partially conjugated (e.g., phenylene vinylene polymer networks). The cross-linked polymer networks may be thin-films disposed on a substrate. The cross-linked polymer network may be covalently bonded to the substrate. The cross-linked polymer networks can be used, for example, in methods of detecting explosives (e.g., RDX (cyclotrimethylenetrinitramine)) and degradation products thereof. 2. The method of claim 1 , wherein the cross-linked polymer network is covalently bonded to the substrate.4. The method of claim 1 , wherein the explosive is selected from nitramines claim 1 , nitroesters claim 1 , degradation products thereof claim 1 , and combinations thereof.5. The method of claim 4 , wherein the nitramine is cyclotrimethylenetrinitramine (RDX) claim 4 , and the nitroester is pentaerythritol tetranitrate (PETN) claim 4 , trinitrotoluene (TNT) claim 4 , dinitrotoluene (DNT) claim 4 , 2 claim 4 ,4 claim 4 ,6-triamino-1 claim 4 ,3 claim 4 ,5-trinitrobenzene (TATB) claim 4 , or 2 claim 4 ,3-dimethyl-2 claim 4 ,3-dinitrobutane (DMNB) claim 4 , octahydro-1 claim 4 ,3 claim 4 ,5 claim 4 ,7-tetranitro-1 claim 4 ,3 claim 4 ,5 claim 4 ,7-tetrazocine (HMX).6. The method of claim 1 , wherein the test sample is a gas-phase sample or a solid sample.7. The method of claim 1 , wherein the sample is not pre-concentrated.8. A thin film of a cross-linked polymer network comprising a plurality of trivinyl benzene moieties disposed on a substrate.9. The thin-film of claim 8 , wherein the substrate is selected from fused silica claim 8 , silicon claim 8 , gold claim 8 , silver claim 8 , platinum claim 8 , copper claim 8 , nickel claim 8 , glass claim 8 , sapphire claim 8 , mica claim 8 , and polymer substrates.10. The thin-film of claim 8 , wherein the cross-linked polymer network is covalently bonded to the substrate.12. The thin film of claim 8 , wherein the film has a thickness of 2 nm to 10 micrometers. This application claims ...

EUROPIUM BASED METAL ORGANIC FRAMEWORK FOR PALLADIUM SENSING

A method of detecting Pd in an aqueous solution is described. The method involves mixing an Eu-based metal organic framework with the aqueous solution and measuring the amount of fluorescence quenching. The fluorescence quenching is specific to Pd and also allows a 44 ppb detection limit of Pd. The Eu-based metal organic framework may be treated with a metal chelator and reused for sensitive detection of Pd. 1: A method of detecting Pd in an aqueous solution , the method comprising:mixing an Eu-based metal organic framework (Eu-MOF) with the aqueous solution to form a solution mixture, wherein the Eu-MOF comprises:{'sup': 3+', '3+', '3+, 'claim-text': [{'sup': '3+', 'wherein each Eu ion cluster is coordinated with a total of 6 linkers, and'}, 'wherein each linker has a dicarboxylic acid and two alkene groups;, 'Eu ion clusters, each Eu ion cluster comprising two Eu ions,'}measuring a fluorescence emission intensity of the solution mixture while irradiating the solution mixture with a wavelength in a range of 320-350 nm; and{'sup': '2+', 'comparing the fluorescence emission intensity to a second fluorescence emission intensity of a substantially similar solution mixture that does not contain Pd.'}2: The method of claim 1 , wherein the alkene groups are part of allyloxy groups.3: The method of claim 2 , wherein the linker is 2 claim 2 ,5-bis(allyloxy)terephthalic acid.4: The method of claim 1 , wherein the linker is present in the Eu-MOF with a weight percent in a range of 55-75 wt % claim 1 , relative to a total weight of the Eu-MOF.5: The method of claim 1 , wherein each Eu ion cluster is coordinated in a 2D layered structure.6: The method of claim 5 , wherein the 2D layered structure has an interlayer spacing in a range of 3.2-3.8 Å.7: The method of claim 1 , wherein the Eu-MOF has an average pore size in a range of 3.5-4.5 Å.8: The method of claim 1 , wherein the Eu-MOF has a pore volume in a range of 0.10-0.20 cm/g.9: The method of claim 1 , wherein the Eu-MOF ...

TARGET SUBSTANCE DETECTION METHOD, TARGET SUBSTANCE DETECTION KIT, AND TARGET SUBSTANCE DETECTION DEVICE

To provide a method for detecting a target substance by using an interaction through a molecular species having low reactivity, with two types of microparticles as proximity probes. A target substance detection method includes the following steps of: a step (A) of bringing a biological sample into contact with an oxygen sensor bound to a first molecule that specifically binds to a target substance; a step (B) of bringing the biological sample into contact with a first solid phase support that holds a second molecule and an oxygen-consuming enzyme, the second molecule specifically binding to the target substance; and a step (C) of acquiring information generated from the oxygen sensor by the steps (A) and (B). 1. A target substance detection method , comprising following steps of:a step (A) of bringing a biological sample into contact with an oxygen sensor bound to a first molecule that specifically binds to a target substance;a step (B) of bringing the biological sample into contact with a first solid phase support that holds a second molecule and an oxygen-consuming enzyme, the second molecule specifically binding to the target substance; anda step (C) of acquiring information generated from the oxygen sensor by the steps (A) and (B).2. The target substance detection method according to claim 1 , wherein in the step (A) claim 1 , the oxygen sensor is held on a second solid phase support.3. The target substance detection method according to claim 1 , wherein the biological sample contains living cells.4. The target substance detection method according to claim 3 , further comprising a step (D) of bringing the living cells into contact with a cell stimulating substance.5. The target substance detection method according to claim 4 , wherein the steps (A) to (D) are performed in a well enabled to capture a single cell.6. The target substance detection method according to claim 1 , wherein the target substance is selected from a group consisting of proteins claim 1 , ...

DEVICE AND METHOD FOR MEASURING THE OXYGEN CONTENT IN WELDING PROCESSES

The invention relates to a device for measuring the oxygen content in welding processes, in particular shielded arc welding, the device having at least one sensor element for sensing the oxygen content of a shielding atmosphere. With the aim of providing a device for measuring the oxygen content in welding processes that provides stable measured values for the residual oxygen content even at very high temperatures of the welding process and is ready to use after a very short time, it is envisaged to design the sensor element as an optical oxygen sensor. 1. A device for measuring the oxygen content in welding processes , in particular shielded arc welding , the device having at least one sensor element for sensing the oxygen content of a shielding atmosphere , characterized in that the sensor element is designed as an optical oxygen sensor.2. The device as claimed in claim 1 , the optical oxygen sensor having a fluorescing medium claim 1 , which can be brought into contact with the shielding atmosphere and is designed for emitting fluorescent light claim 1 , the intensity of the emitted fluorescent light being dependent on the oxygen partial pressure in the shielding atmosphere.3. The device as claimed in claim 2 , the optical oxygen sensor having at least one device for optically exciting the fluorescing medium and at least one optical detector for sensing electromagnetic radiation that is given off by the fluorescing medium as a result of a deactivation process.4. The device as claimed in claim 3 , the device for exciting the fluorescing medium being designed to give off an excitation pulse to the fluorescing medium at previously determinable time intervals.5. The device as claimed in claim 3 , the device having an evaluation unit claim 3 , which is connected to the optical detector and is designed for determining the intensity or the decay behavior of the fluorescent radiation of the fluorescing medium.6. The device as claimed in claim 5 , the evaluation unit ...

SYSTEMS AND METHODS FOR CONFIRMING ACTIVATION OF BIOLOGICAL INDICATORS

Biological indicators may be improperly activated. The disclosed subject matter is directed to methods of confirming that a biological indicator having an ampule containing a growth medium has been properly activated such that it may be assayed. The methods may include the steps of measuring a first fluorescence intensity of the biological indicator, heating the biological indicator; quenching the fluorescence intensity of the biological indicator from the first fluorescence intensity to a second fluorescence intensity, measuring the second fluorescence intensity; comparing the second fluorescence intensity and first fluorescence intensity to obtain a comparison value; and determining that the comparison value corresponds to a quenching metric of the liquid growth medium. 1. A method for assessing a status of a biological indicator containing an ampule having a liquid growth medium , comprising:depressing a cap of the biological indicator;breaking the ampule;positioning the biological indicator into a biological indicator analyzer having a heating element, a light source, and a fluorescence sensor;activating the heating element;measuring a first fluorescence intensity of the biological indicator;quenching the fluorescence intensity of the biological indicator from the first fluorescence intensity to a second fluorescence intensity;measuring the second fluorescence intensity;comparing the second fluorescence intensity and first fluorescence intensity to obtain a comparison value; anddetermining that the comparison value corresponds to a quenching metric of the liquid growth medium.2. The method of claim 1 , wherein the step of quenching the fluorescence intensity of the biological indicator includes heating the biological indicator.3. The method of claim 1 , wherein the step of quenching the fluorescence intensity of the biological indicator includes heating the growth medium and the housing of the biological indicator from a temperature of between approximately 22 ...

Optically-based nanopore analysis with reduced background

The invention is directed to nanopore arrays comprising opaque layers that reduce background fluorescence in optical signal collected in applications of such arrays for analyzing molecules. In some embodiments, such arrays are used to determine characteristics of polymers, such as polynucleotides, in methods comprising the steps of translocating polymers through nanopores of such arrays wherein polymers have one or more optical labels, exciting optical labels of the polymers in a signal generation region of each nanopore extending from the opaque layer toward the direction of the excitation beam, detecting optical signals from the signal generation regions of each nanopore to determine characteristics of the polymer translocating therethrough.

Methods and Systems for Enhanced Microfluidic Processing

Methods and systems are provided for a microfluidic cartridge including a high performance actuator useful for analyte detection, labeling and analysis. Microfluidic processing systems are to carry out chemical or biochemical reactions, or sequences of reactions, with small volumes (typically between 1 microliter and 10 milliliters) of reactants and products. A microfluidic processing system can comprise a network of tubes interfaced with discrete components such as valves and sensors, or an integrated device made of plastic, glass, metal, or other materials, or a combination of materials, with components such as valves and sensors built into the device and connected by flow passageways formed in the material.

METHOD FOR PREPARING A RATIOMETRIC FLUORESCENT SENSOR FOR PARACETAMOL BASED ON A COPPER NANOCLUSTERS-CARBON DOTS-ARGININE COMPOSITE

A method for preparing a ratiometric fluorescent sensor for paracetamol based on a copper nanoclusters-carbon dots-arginine composite is provided. Copper nanoclusters CuNCs with red fluorescence are bonded to carbon dots (CDs) with blue fluorescence by electrostatic adsorption and hydrogen bonding, and then arginine is added to form the CuNCs-CDs-arginine composite. The addition of arginine leads to a significant decrease in the blue fluorescence of the CDs, while after the paracetamol is added, the blue fluorescence of the CDs gradually recovered as a result of the specific binding of arginine to paracetamol. The ratiometric fluorescent sensor for paracetamol is constructed by taking the fluorescence of the CuNCs as a reference signal, and the fluorescence of the CDs as a response signal, and fitting the linear relationship between the ratios I/Iof fluorescence emission peak intensities of CDs and CuNCs and the molar concentrations of paracetamol. 1. A method for preparing a ratiometric fluorescent sensor for paracetamol based on a copper nanoclusters-carbon dots-arginine composite , comprising the following steps:{'sub': 2', '2, '(1) preparation of CuNCs: dissolving DNA dry powder in ultrapure water to obtain a DNA solution, diluting the DNA solution to a pH of 7.5 with a buffer solution containing 3-morpholine propane sulfonic acid and NaCl to obtain a DNA mixed solution, wherein a concentration of the 3-morpholine propane sulfonic acid is 10 mM and a concentration of the NaCl is 15 mM; adding ascorbic acid to the DNA mixed solution to obtain a first mixed solution, wherein a final concentration of the ascorbic acid is adjusted to 2 mM; at room temperature and stirred magnetically, adding CuClto the first mixed solution to obtain a second mixed solution, wherein a final concentration of the CuClis adjusted to 1 mM; performing a reaction on the second mixed solution away from light for 20 minutes to obtain a first product solution; dialyzing the first product ...

SYSTEM AND METHOD FOR DETECTION AND SIGNALING OF COMPONENT END-OF-LIFE IN A DISSOLVED OXYGEN SENSOR

Embodiments as disclosed herein may include a sensor including a luminophor exposed to a fluid flow path. The luminophor may emit light in response to illumination by an excitation light source. The magnitude of light emitted by the luminophor in response to illumination may be determined. It can be determined if this magnitude is within a threshold of the baseline magnitude and an alarm state set based on this determination. This alarm state may indicate that the luminophor has reached an end-of-life state or otherwise should be replaced. 1. A dissolved oxygen sensor , comprising:a window of optically transparent material wherein a luminophor is attached to a first side of the window capable of being exposed to a fluid; an excitation light source configured to illuminate the luminophor;', 'a photodiode configured to receive light emitted by the luminophor in response to illumination; and', determine a magnitude of the light emitted from the luminophor;', 'determine if the magnitude of the light emitted from the luminophor is within a threshold of the baseline magnitude; and', 'set an alarm state associated with the luminophor based on the determination if the magnitude of the light emitted from the luminophor is within a threshold of the baseline magnitude., 'an electronic component having a baseline magnitude associated with the luminophor stored therein and configured to], 'an optical probe opposite the window from the fluid flow path and the luminophor, wherein the optical probe includes2. The dissolved oxygen sensor of claim 1 , wherein the threshold is 50%.3. The dissolved oxygen sensor of claim 1 , wherein the baseline magnitude is determined during calibration of the dissolved oxygen sensor.4. The dissolved oxygen sensor of claim 1 , wherein the calibration occurs at atmosphere.5. The dissolved oxygen sensor of claim 1 , wherein the electronic component is configured to determine a measure of oxygen concentration based on the light emitted by the luminophor. ...

Long-Throw Microfluidic Actuator

A microfluidic device includes a three-dimensional slat structure having a plurality of interstices configured to generate a high power, high flow rate of fluids by electroosmotic flow. The microfluidic device includes a housing for holding and moving fluids through the slat structure, and a plurality of electrodes that generate an electric field within the plurality of interstices. 156.-. (canceled)57. A microfluidic cartridge comprising:a plurality of fluid passageways;at least one junction connecting said plurality of fluid passageways; and {'sup': '−8', 'a fluid power generation capacity of at least 10watts and capable of sustaining said power for at least 30 seconds; and'}, 'at least two fluid transport means, including at least one high-performance fluidic actuator, the at least one high-performance fluidic actuator being a discrete component within the cartridge, and the at least one high-performance fluidic actuator havinga response time for fluid power generation of less than 10 seconds.58. The cartridge of claim 57 , wherein said cartridge has a displaced volume less than or equal to five hundred cubic centimeters or less than or equal to fifty cubic centimeters.59. The cartridge of claim 57 , wherein said at least one high-performance fluidic actuator is capable of transducing electrical power into fluidic power.60. The cartridge of claim 57 , wherein said actuator is capable of pressurizing at least 10 microliters of liquid claim 57 , such that said liquid flows through a fluidic resistance associated with a back pressure of at least 1 kPa at a flow rate of at least 0.1 mL per minute.61. The cartridge of claim 57 , wherein said high-performance actuator is coupled to a pulse generator or other controlled time-varying voltage source and at least one electrode.62. The cartridge of claim 57 , wherein said at least one high-performance fluidic actuator is capable of producing fluidic power through an electrokinetic effect.63. The cartridge of claim 62 , ...

Multi-Channel Optical Measurement Instrument

A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. 1. A detector for detecting optical emissions of two or more different wavelengths or ranges of wavelengths from a sample , wherein emissions of two or more different wavelengths are indicative of the presence , amount , or state of two or more analytes of interest in the sample , the detector comprising: a light emitting element adapted to emit excitation light; and', 'excitation optical elements defining an excitation optical path having an excitation optic axis, the excitation optical elements being constructed and arranged to transmit at least a portion of the light emitted by the light-emitting element having the prescribed excitation wavelength or range of excitation wavelengths toward the sample;, 'two or more excitation channels fixed with respect to the sample and each other, wherein each excitation channel is adapted to direct an excitation signal of a different prescribed excitation wavelength or range of excitation ...

ANALYTICAL INSTRUMENT SYSTEMS

The invention provides optical instrument systems and methods for analyzing signals from biological arrays, and performing analytical amplification reactions for identifying the presence or absence of a target nucleic acid sequence in a sample to be analyzed. 1. A detection system , comprising:an excitation light source;a reaction vessel comprising an array of capture probe sites disposed thereon, the array producing one or more fluorescent signals in response to the excitation light;an image sensor;an optical train for transmitting excitation light from the excitation light source to the array, and fluorescent signals from the array to the image sensor;one or more thermal control elements disposed in thermal communication with the reaction vessel; anda processor operably coupled to the one or more thermal control elements, for subjecting contents of the reaction vessel to a thermal cycling profile.2. The system of claim 1 , wherein the optical train includes a focusing lens for focusing the fluorescent signals onto the image sensor claim 1 , and an optical path length adjustment component between the focusing lens and the image sensor.3. The system of claim 2 , wherein the optical path length adjustment component comprises a rotatable variable thickness disk.4. The system of claim 3 , wherein the rotatable variable thickness disk comprises a transparent material selected from glass claim 3 , quartz claim 3 , fused silica claim 3 , and a transparent polymer.5. The system of claim 4 , wherein the transparent polymer is selected from polymethylmethacrylate claim 4 , poly(carbonate) claim 4 , poly(styrene) claim 4 , poly(ethersulfone) claim 4 , poly(aliphatic ether) claim 4 , halogenated poly(aliphatic ether) claim 4 , poly(aryl ether) claim 4 , halogenated poly(aryl ether) claim 4 , poly(amide) claim 4 , poly(imide) claim 4 , poly(ester)poly(acrylate) claim 4 , poly(methacrylate) claim 4 , poly(olefin) claim 4 , halogenated poly(olefin) claim 4 , poly(cyclic olefin) ...

Waveguide sensor with nanoporous surface layer

A waveguide sensor system is provided. The system includes a light source and a waveguide formed from a light transmitting material. Light from the light source enters the waveguide at an input area and travels within the waveguide by total internal reflection to an analyte area and light to be analyzed travels within the waveguide from the analyte area by total internal reflection to an output area. An optical sensor is coupled to the output area and is configured to interact with the light to be analyzed. The system includes a plurality of pores located along the outer surface within the analyte area and formed in the light transmitting material of the waveguide, and the pores are configured to enhance light interaction with the analyte within the analyte area.

Method and sensor array for identifying an analyte

A method and sensor array for identification of an analyte is disclosed. The method comprises preparing a plurality of solutions at a plurality of pH values of at least one fluorescent poly(para-phenyleneethynylene) and its complex(es), exposing the complex analyte to the plurality of the solutions and measuring the fluorescence intensity of the exposed complex analyte. The fluorescence intensity is compared with a library and the complex analyte identified from the comparison. 1. A method for identification of an analyte comprising:preparing a plurality of solutions at a plurality of pH values of at least one fluorescent poly(para-phenyleneethynylene) and its complex;exposing the complex analyte to the plurality of the solutions;measuring the fluorescence intensity of the exposed complex analyte;comparing the fluorescence intensity with a library; andidentifying the complex analyte from the comparison.2. The method of claim 1 , wherein the analyte is at least one of a fruit juice or an active pharmaceutical preparation.3. The method of claim 1 , wherein the poly(para-phenyleneethynylene) is selected from one of the poly(para-phenyleneethynylene)s shown in or .4. A sensor array for the identification of an analyte comprising:a plurality of wells having solutions at a plurality of pH values of at least one fluorescent poly(para-phenyleneethynylene) and its complex;an excitation light source;a fluorescent light detector; anda storage device for recording a plurality of fluorescent light patterns.5. The sensor array of claim 4 , further comprising a processor adapted to accept measured fluorescent light intensity from the fluorescent light detector and compare the measured fluorescent light intensity with the plurality of stored fluorescent light patterns.6. The sensor array of claim 4 , wherein the poly(para-phenyleneethynylene) is selected from one of the poly(para-phenyleneethynylene shown in or . This application claims benefit to and priority of UK Patent ...

OPTICAL SENSOR ELEMENT

The invention relates to an optical sensor element, comprising indicators, selected from luminescence-active means that are of the same type or different, and indicator protectors, and to a sensor, comprising at least one such sensor element, an energy source that excites the luminescence emission of the indicators, and a detector unit, wherein the sensor element or sensor is suitable for detecting molecular oxygen in a gaseous or liquid medium and/or for determining the molecular oxygen content of a gaseous or liquid medium and at least one layer of the sensor element bearing the indicator protectors is designed in such a way that the diffusion rate of the molecular oxygen formed on the indicator protectors by means of the reduction of strong oxidants back into the medium is greater than the diffusion rate of molecular oxygen from the medium in the direction of the at-least-one layer bearing the indicator molecules. 1. An optical sensor element comprising:at least one indicator composed of a luminescence-active agent; andat least one indicator protector selected from (a) reducing agents and/or catalysts for the reduction of strong oxidants, (b) adsorbents for chemisorption or physisorption of strong oxidants, or (c) a combination of (a) and (b),wherein the element has at least two layers, and the at least one indicator and the at least one indicator protector are arranged in different layers of the element, and the element comprises at least one indicator-bearing layer and at least one indicator protector-bearing layer, andwherein the at least one indicator protector is arranged in a layer of the element that faces towards the medium and the at least one indicator is arranged in at least one layer that is mounted facing away from the medium.2. The optical sensor element according to claim 1 , wherein the at least one indicator protector-bearing layer is substantially free of indicators.3. The optical sensor element according to claim 1 , wherein the at least one ...

SINGLE MOLECULE TIMERS AND CLOCKS

The present disclosure provides, in some aspects, single-molecule timers and clocks, systems and methods for kinetically encoded imaging. 1. A kinetically encoded imaging system , comprising:(a) an unpaired initiator nucleic acid comprising a 3′ nucleotide subdomain and a 5′ nucleotide subdomain;(b) a template probe comprising (i) an unpaired 5′ toehold domain, (ii) a hairpin stem domain formed by base pairing between nucleotides located in a 5′ subdomain of the probe and nucleotides located in a 3′ subdomain of the probe, and a hairpin loop domain; and(c) a primer.2. The system of claim 1 , wherein the primer is linked to a detectable molecule.3. The system of claim 1 , wherein the template probe is linked to a detectable molecule.4. The system of claim 1 , wherein the 3′ nucleotide subdomain of the initiator of (a) is complementary to and binds to the unpaired toehold domain of the probe of (b) claim 1 , and the 5′ nucleotide subdomain of the initiator of (a) is complementary to and binds to the 5′ subdomain of the probe of (b).5. The system of claim 1 , wherein the primer is complementary to and binds to the 3′ subdomain of the probe of (b).6. The system of claim 1 , wherein the template probe further comprises 3′ phosphate (PO) group.7. The system of further comprising a DNA polymerase.8. The system of claim 7 , wherein the DNA polymerase has strand displacement activity.9. The system of claim 8 , wherein the DNA polymerase is phi29.10. The system of claim 8 , wherein the DNA polymerase is Bst DNA polymerase claim 8 , large fragment.11. The system of claim 1 , wherein the initiator nucleic acid has a length of 15-50 nucleotides.12. The system of claim 11 , wherein the initiator nucleic acid has a length of 20-30 nucleotides.13. The system of claim 11 , wherein the 3′ nucleotide subdomain of the initiator nucleic acid has a length of 5-15 nucleotides.14. The system of claim 11 , wherein the 5′ nucleotide subdomain of the initiator nucleic acid has a length of 10- ...

PARALLEL POLYMER SEQUENCING METHODS

The present invention relates to a method of sequencing a target polynucleotide by enzymatic and/or chemical means. The sequencing method includes a method for characterizing multiple alleles in a sample, a method of calculating confidence levels in ascertained sequences, a method for comparing polynucleotide sequences and a method of resolving ambiguities in a polynucleotide sequence. It also provides methods for appropriately preparing samples, for immobilising template molecules, for organising the template molecules and to conduct the sequencing of many molecules in parallel. The method involves analysing molecules as members of an array. Many target polynucleotides or many segments of a single target polynucleotide can be sequenced simultaneously. In a preferred embodiment the method involves analysing individual molecules within an array and base calls are based on the signals from two or more molecules. A method to prevent non-specific signal in sequencing is also provided. The invention is readily automated, both for small-scale and large-scale operation and relevant algorithms and the composition of kits and systems are provided. 157-. (canceled)58. A method of sequencing a target polynucleotide comprising:(a) incorporating a plurality of intercalating dye molecules into the target polynucleotide;(b) contacting the target polynucleotide with a solution comprising a polymerase and four types of differently labeled nucleotides, wherein the labels each comprise a fluorescence resonance energy transfer (FRET) partner to the intercalating dye molecules, and wherein the labeled nucleotides each comprise a terminator group;(c) incorporating one of the differently labeled nucleotides, using the polymerase, into a chain complementary to the target polynucleotide;(d) conducting FRET on the intercalating dye and incorporated differently labeled nucleotide partners, thereby identifying the type of the differently labeled nucleotide incorporated;(e) removing the ...

LIGAND DETECTION BY APTAMERS WITH A BUILT-IN REPORTER

Potassium ion sensing aptamers are disclosed. These aptamers have high specificity towards the potassium ion. Ligand-sensing aptamers with a built-in reporter are also disclosed. The built-in reporter is incorporated into the sugar-phosphate backbone of the aptamers. The built-in reporter may be an environmentally sensitive fluorescence dye, internally coupled to the aptamers. The environmentally sensitive fluorescence dye can sense the conformation changes induced by binding of the aptamers to the target ligand and transduces the conformational changes to a fluorescence change. 2. The oligonucleotide of claim 1 , having between 14 and 22 claim 1 , inclusive claim 1 , nucleotides claim 1 , and/or wherein the oligonucleotide is a DNA oligonucleotide.3. (canceled)4. A reporter-containing oligonucleotide claim 1 , comprising(a) a nucleotide sequence in which at least 40, 45, 50, 55, 60, 65, or 70 percent of the nucleotides are guanine nucleotides; and(b) a fluorescence dye as a built-in reporter,wherein the fluorescence dye is incorporated in the sugar-phosphate backbone of the oligonucleotide,wherein the oligonucleotide optionally undergoes a conformational change upon binding to a ligand of the oligonucleotide, wherein the conformational change of the oligonucleotide induces a photophysical change of the fluorescence dye.5. The reporter-containing oligonucleotide of claim 4 , wherein the oligonucleotide forms a G-quadrulex upon binding the ligand.7. The reporter-containing oligonucleotide of claim 4 , having between 14 and 22 claim 4 , inclusive claim 4 , nucleotides and/or wherein the oligonucleotide is a DNA oligonucleotide.8. (canceled)9. The reporter-containing oligonucleotide of claim 4 , wherein the fluorescence dye is an environmentally sensitive fluorescence dye.10. The reporter-containing oligonucleotide of claim 9 , wherein the environmentally sensitive fluorescence dye is a cyanine dye.12. (canceled)13. The reporter-containing oligonucleotide of claim 4 , ...

MULTIPLEX DETECTION OF NUCLEIC ACIDS

The present invention provides oligonucleotides and methods for their use in the detection and/or differentiation of target nucleic acids. The oligonucleotides and methods find particular application in amplifying, detecting, and/or discriminating multiple targets simultaneously. 1. A method for determining the presence or absence of first and second targets in a sample , the method comprising:(a) preparing a reaction mixture by contacting the sample or a derivative thereof putatively comprising the first and/or second targets or amplicons thereof with:first and second closed stem-loop oligonucleotides, wherein each of the closed stem-loop oligonucleotides comprise a double-stranded stem portion of hybridised nucleotides connected to a closed single-stranded loop portion of unhybridised nucleotides, wherein:the number of hybridised nucleotides and/or the sequence of hybridised nucleotides in the stem portion of the first and second closed stem-loop oligonucleotide differs, andenzymes capable of cleaving or degrading the single-stranded loop portion of the first and second closed stem-loop oligonucleotides only when in contact with the target or an amplicon thereof; under conditions suitable for the enzymes to induce cleavage or degradation of the loop portion of the first and second closed stem-loop oligonucleotides to thereby produce first and second open stem-loop oligonucleotides;', 'at a first temperature at or above which strands of the double-stranded stem portion of the first open stem-loop oligonucleotide disassociate to thereby facilitate spatial separation of the fluorophore and quencher molecules of the stem portion of the first open stem-loop oligonucleotide and provide a first detectable fluorescent signal, and', 'at a second temperature at or above which strands of the double-stranded stem portion of the second open stem-loop oligonucleotide disassociate to thereby facilitate spatial separation of the fluorophore and quencher molecules of the stem ...

METHOD FOR PREPARING RATIOMETRIC FLUORESCENT PROBE FOR MELAMINE BASED ON SILVER NANOCLUSTER COMPLEX

A method for preparing a ratiometric fluorescent probe for melamine based on a DNA-stable silver nanocluster-rhodamine 6G complex, wherein the electrostatic self-assembly technology is adopted to construct a silver nanocluster-rhodamine 6G complex. The melamine forms a strong hydrogen bond with thymine in the DNA of the surface of the silver nanocluster, causing rhodamine 6G to dissociate from the surface of the silver nanocluster, destroying the fluorescence resonance energy transfer, so as to restore the fluorescence of the silver nanocluster. This process has little effect on Rhodamine 6G fluorescence which can be used as a reference signal, while silver nanocluster fluorescence can be used as a response signal. By fitting the linear relationship between the ratio of fluorescence emission peak intensities of the silver nanocluster to rhodamine 6G and the molar concentration of the melamine, the ratiometric fluorescent probe for melamine can be constructed. 1(1) preparation of DNA-stable silver nanoclusters: at 0° C., adding a predetermined amount of silver nitrate solution and DNA solution to 1 mL of double-distilled water to obtain a first solution, stirring the first solution magnetically for 20 minutes to form a homogeneous mixture, then adding a sodium borohydride solution to the homogeneous mixture to obtain a second solution, and performing a reaction on the second solution under a vigorous stirring in a dark place for 20 minutes to obtain a first product solution; wherein the first product solution is filtered by a 0.4 μm filter to obtain a filtrate, and the filtrate is dialyzed through a dialysis bag with a molecular weight cut-off of 5000 Daltons to remove unreacted experimental materials; a solution in the dialysis bag is subjected to a rotary evaporation to remove 90% of a solvent, and then freeze-dried to obtain a dry sample of the DNA-stable silver nanoclusters; the dry sample of the DNA-stable silver nanoclusters is stored at 4° C. in a dark ...

SENSOR MEMBRANE, SENSOR CAP AND/OR OPTICAL SENSOR AND METHOD FOR MANUFACTURING A SENSOR MEMBRANE

A sensor membrane for an optical sensor, wherein the outer layer in contact with the medium and/or a layer adjacent thereto has a graft copolymer to form an omniphobic surface in contact with the medium, as well as a sensor cap and/or an optical sensor and a method for manufacturing the sensor membrane. 1. A sensor membrane for an optical sensor , comprising:an outer layer embodied to contact a liquid medium,wherein the outer layer includes a graft polymer or a graft copolymer forming an omniphobic surface in contact with the liquid medium.2. The sensor membrane according to claim 1 , wherein the outer layer further includes:microstructure-forming particles, anda nanostructure grafted onto the microstructure-forming particles.3. The sensor membrane according to claim 2 , wherein the microstructure-forming particles are spherical or capsule-shaped components of the outer layer.4. The sensor membrane according to claim 1 , wherein the omniphobic surface has a pin cushion structure or a honeycomb structure.5. The sensor membrane according to claim 2 ,wherein the outer layer includes a reactive matrix material,wherein the microstructure-forming particles are immobilized in the outer layer and form a microstructure,wherein the nanostructure is formed as grafted side chains covalently bound to the reactive matrix material located on the microstructure or covalently bound directly on the microstructure, andwherein the microstructure is selected from a group consisting of silicon oxide particles, titanium oxide particles, polystyrenes, styrene polymers, polybutadiene, natural substance capsules, and hybrids of the aforementioned compounds.6. The sensor membrane according to claim 5 ,wherein the microstructure-forming particles are scaffold structures of diatoms or exines of spores or exines of pollen.7. The sensor membrane according to claim 5 , wherein the microstructure is formed by:grafting microstructure-forming particles comprising polybutadiene or polybutadiene-coated ...

FLUORESCENT PROBE FOR DETECTING NITROREDUCTASE AND PREPARATION METHOD AND USE THEREOF IN ENZYMATIC REACTION

The present invention relates to a fluorescent probe for detecting nitroreductase and a preparation method and use thereof in enzymatic reactions, belonging to the field of industrial analysis and detection. The fluorescent probe is 3-(4-(2-(4′-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1,1′-biphenyl]-4-yl)vinyl)quinolin-1-ium-1-yl)propane-1-sulfonate. The fluorescent probe of the present invention, with the introduction of hydrophilic groups, sulfonate and quinolinium, the probe's hydrophilicity is enhanced, under the enzymatic catalysis of nitroreductase (NTR), 1,6-rearrangement and elimination reaction occurs, and hydroxyl group is generated. Detection and analysis of the NTR in the industrial enzymatic reactions can be realized due to the change of fluorescence which is induced by the intramolecular charge transfer (ICT) effect. This method has such advantages as easy preparation, high yield and being suitable for detecting high concentration of enzyme in the enzymatic reactions, and it shows an extensive application prospect in the field of enzyme-detection in the industrial enzymatic reaction systems. 2. A preparation method of the fluorescent probe for detecting nitroreductase according to claim 1 , wherein comprising the following steps:(1) dissolving 4′-(diphenylamino)-3-hydroxy-[1,1′-biphenyl]-4-carbaldehyde into dimethyl sulfoxide and dissolving 1-(bromomethyl)-4-nitrobenzene into tetrahydrofuran, followed by ultrasonic treatment respectively and then mixing together, adding cesium carbonate, controlling a reaction temperature in the range of 50° C.-150° C., separating and purifying a reaction product to obtain 4′-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1,1′-biphenyl]-4-carbaldehyde in yellow solid powder;(2) dissolving 3-(4-methylquinoline-1-bromine)propane-1-sulfonate into pyridine, then adding acetic acid, followed by sufficient mixing, then adding the 4′-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1,1′-biphenyl]-4-carbaldehyde obtained in step (1), heating ...

SELECTIVE DELIVERY MOLECULES AND METHODS OF USE

Disclosed herein is a selective delivery molecule comprising: (a) an acidic sequence (portion A) which is effective to inhibit or prevent the uptake into cells or tissue retention, (b) a molecular transport or retention sequence (portion B), and (c) a linker between portion A and portion B, and (d) at least one cargo moiety. 1. A selective delivery molecule of Formula I , having the structure:{'br': None, 'sub': A', 'A', 'M', 'B', 'B, '[D-c]-A-[c-M]-X-B-[c-D]\u2003\u2003Formula I'} X is a cleavable linker;', 'A is a peptide with a sequence comprising 5 to 9 acidic amino acids;', 'B is a peptide with a sequence comprising 7 to 9 basic amino acids;', {'sub': A', 'B', 'M, 'c, c, and care each independently 0-1 amino acid;'}, 'M is a polyethylene glycol (PEG) polymer; and', {'sub': A', 'B, 'Dand Dare each independently an imaging agent; and'}], 'wherein,'}{'sub': M', 'A', 'A', 'B', 'B, 'wherein [c-M] is bound to at any position on A or X, [D-c] is bound to any amino acid on A, and [c-D] is bound to any amino acid on B.'}2. The molecule of claim 1 , wherein A and B do not have an equal number of acidic and basic amino acids.3. The molecule of claim 2 , wherein the number of basic amino acids in B is greater than the number of acidic amino acids in A.4. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates.5. The molecule of claim 1 , wherein B is a peptide comprising 8 or 9 consecutive arginines.6. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates and B is a peptide comprising 8 or 9 consecutive arginines.7. The molecule of claim 6 , wherein A is a peptide comprising 5 consecutive glutamates and B is a peptide comprising 8 consecutive arginines.8. The molecule of claim 1 , wherein c claim 1 , c claim 1 , and care each independently selected from a naturally-occurring amino acid or a non-naturally-occurring amino acid.9. The molecule of claim 8 , wherein c claim 8 , c claim 8 , and care each ...

METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING

A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values. 15-. (canceled)6. A method for analyzing a blood sample quiescently residing within an analysis chamber , which sample has at least one first constituent and at least one second constituent , and which second constituent is a different type than that of the first constituent , and which sample is mixed with a colorant , the method comprising:illuminating at least a portion of the sample containing the first constituent and the second constituent with light at one or more wavelengths that cause the colorant to fluoresce, and with light at one or more wavelengths that are absorbed by the colorant;imaging the at least a portion of the sample, including producing first image signals indicative of fluorescent emissions from the colorant disposed within the first constituent and second image signals from the colorant disposed within the second constituent, and including producing third image signals indicative of the optical density of at least one region within the first constituent and producing fourth image signals indicative of the optical density of at least one region within the second constituent;determining at least one value representative of fluorescent emissions using the first image signals and at least one value representative of fluorescent emissions using the ...

ISOTHERMAL AMPLIFICATION COMPONENTS AND PROCESSES

The technology relates in part to methods and compositions for isothermal amplification of nucleic acids. 124.-. (canceled)25. A method for detecting a target nucleic acid sequence in a sample , the method comprising: (i) a first primer and a second primer, wherein the first primer is capable of hybridizing to a sequence of the first strand of the target nucleic acid sequence, and the second primer is capable to hybridizing to a sequence of the second strand of the target nucleic acid sequence; and', (1) the sequence of the first primer or a portion thereof, or the reverse complement thereof,', '(2) the sequence of the second primer or a portion thereof, or the reverse complement thereof, and', '(3) a spacer sequence flanked by (1) and (2), wherein the spacer sequence is 1 to 10 bases long; and, '(ii) an enzyme having a hyperthermophile polymerase activity, thereby generating a nucleic acid amplification product, wherein the nucleic acid amplification product comprises], '(a) amplifying a target nucleic acid sequence in a sample under an isothermal amplification condition, wherein the target nucleic acid sequence comprises a first strand and a second strand complementary to each other, and wherein the amplifying comprises contacting a double-stranded nucleic acid comprising the target nucleic acid sequence with(b) detecting the nucleic acid amplification product, wherein the detecting is performed in 20 minutes or less from the time the double-stranded nucleic acid is contacted with (a)(i) the first and second primers and (a)(ii) the enzyme having a hyperthermophile polymerase activity,wherein the method does not comprise using any enzymes that is not a polymerase.26. The method of claim 25 , wherein the amplifying under the isothermal amplification condition does not comprise using any enzymes other than the enzyme having a hyperthermophile polymerase activity.27. The method of claim 25 , wherein the double-stranded nucleic acid is a genomic nucleic acid claim 25 , ...

METHOD OF CAPTURING AND STABILISING THIOLS

The present invention relates to a method of capturing and stabilising volatile thiol malodour generated on the human skin, comprising steps of: i) contacting the human skin with a substrate, ii) absorbing said thiol into the substrate, and iii) reacting said thiol with a thiol-capture agent. The invention also relates to a method of quantifying said thiol, a method of assessing the deodorizing performance of a cosmetic composition on the human skin. 1. A method of capturing and stabilising volatile thiol malodour generated on the human skin , said method comprising steps of:i) contacting the human skin with a substrate,ii) absorbing thiol malodour into the substrate, andiii) reacting thiol malodour with a thiol-capture agent.2. The method according to claim 1 , wherein the thiol-capture agent is present on the substrate when it contacts the human skin.3. The method according to claim 1 , wherein the thiol-capture agent is subsequently added to the substrate after step (ii).4. The method according to claim 1 , wherein the thiol-capture agent is subsequently added to a solvent extraction taken from the substrate after step (ii).6. The method according to claim 5 , wherein Xis a leaving group selected from I claim 5 , Br claim 5 , Cl claim 5 , sulphonate or substituted sulphonate and Xis also a leaving group selected from I claim 5 , Br claim 5 , Cl claim 5 , sulphonate or substituted sulphonate or is a hydrogen.7. The method according to claim 6 , wherein both Xand Xare leaving groups selected from I claim 6 , Br claim 6 , Cl claim 6 , sulphonate or substituted sulphonate.8. The method according to claim 5 , wherein the thiol-capture agent is a compound having a formula (I) and Ris a hydrogen or polyethylene glycol.9. The method according to claim 8 , wherein the thiol-capture agent is a compound having a formula (I) and wherein Ris a hydrogen or Polyethylene glycol and both Xand Xare Br.10. The method according to claim 9 , wherein the thiol-capture agent is a ...

Portable Device for Detecting Explosive Substances Comprising a Device for Generating and Measuring the Emission of an Indicator

A portable appliance for detecting explosive materials has an apparatus for generating and measuring emission of an indicator. The indicator has a carrier and a plurality of indicator substances that can be impinged upon by analytes, said indicator substances being applied onto the carrier as a thin layer and being positionable in an indicator area by means of a holding apparatus. The indicator substances are arranged in different regions of the carrier such that the indicator substances in the indicator area represent a pattern of indicator substances depending on the location. The apparatus has a plurality of radiation sources for emitting quasi-monochromatic excitation radiation. The radiation sources are arranged at various locations in a radiation area such that the radiation area represents a pattern of radiation sources depending on the location. The apparatus has an excitation beam path with at least one first imaging system for imaging excitation radiation into the indicator area such that emission of the indicator substances is producible at the locations at which the excitation radiation is imaged on the indicator area. An emission beam path has at least one second imaging system for imaging the indicator area into a reception area such that a pattern of emissions is producible in the reception area depending on the location. A plurality of receivers receive emissions from the reception area and for converting the received emissions into electrical signals. 1. (canceled)2. A portable appliance for detecting explosive materials , comprising:an apparatus for generating and measuring emission of an indicator, whereinthe indicator has a carrier and a plurality of indicator substances that can be impinged upon by analytes,the indicator substances are applied onto the carrier as a thin layer and are positionable in an indicator area by a holding apparatus,the indicator substances are arranged in different regions of the carrier such that the indicator ...

CONTINUOUS PROCESS FOR PERFORMING MULTIPLE NUCLEIC ACID AMPLIFICATION ASSAYS

A method of determining the presence or amount of a target nucleic acid in each of a plurality of reaction mixtures. In the method, a first plurality of reaction mixtures are provided to a heater and subjected to conditions for performing a first amplification reaction. The presence or amount of a first target nucleic acid in each of the first plurality of reaction mixtures is determined during the first amplification reaction. During the first amplification reaction, a second plurality of reaction mixtures are provided to the heater and subjected to conditions for performing a second amplification reaction. The presence or amount of a second target nucleic acid in each of the second plurality of reaction mixtures is determined during the second amplification reaction. At least a portion of the second plurality of reaction mixtures are removed from the heater during the second amplification reaction. 1. (canceled)2. A method of determining the presence or amount of a target nucleic acid in each of a plurality of reaction mixtures , the method comprising the automated steps of:a) providing a first plurality of reaction mixtures to a heater disposed on a generally horizontal work surface of an analyzer having a window situated above the work surface, wherein each reaction mixture of the first plurality of reaction mixtures is suspected of containing a first target nucleic acid, and wherein each reaction mixture of the first plurality of reaction mixtures contains reagents sufficient to perform a first nucleic acid amplification reaction with the first target nucleic acid;b) in the heater, subjecting each reaction mixture of the first plurality of reaction mixtures to conditions sufficient to perform the first nucleic acid amplification reaction;c) determining the presence or amount of the first target nucleic acid in each reaction mixture of the first plurality of reaction mixtures during the first nucleic acid amplification reaction;d) during steps b) and c), ...

PROTECTIVE DEVICE FOR AN OPTOCHEMICAL SENSOR, AND CORRESPONDING OPTOCHEMICAL SENSOR

The present disclosure relates to a protective device for a sensor unit of an optochemical sensor for determining or monitoring at least one analyte present in a medium, including at least one protective substance for protecting the sensor unit against a physical and/or chemical alteration caused by at least one substance contained in the medium, and an at least partially media-permeable functional layer, where the protective device, in a region facing towards the medium, can be attached to the sensor unit or applied to the sensor unit. The present disclosure further relates to an optochemical sensor having a protective device according to the present disclosure. 1. A protective device for a sensor unit of an optochemical sensor for determining or monitoring at least one analyte present in a medium , the protective device comprising:at least one protective substance for protecting the sensor unit against a physical and/or chemical alteration caused by at least one substance contained in the medium; andan at least partially media-permeable functional layer,wherein the protective device, in a region facing towards the medium, is embodied to be attached to the sensor unit or applied to the sensor unit.2. The protective device of claim 1 , wherein the at least one protective substance is:a buffer, including a pH buffer polymer, a pH buffer solution, a redox buffer, and/or a redox buffer polymer;an adsorbent;a radical scavenger;a reducing agent;a catalyst; ora polymer.3. The protective device of claim 2 , wherein the polymer of the at least one protective substance is an acrylamide or an acrylamide with at least one imidazole unit.4. The protective device of claim 1 , wherein the functional layer is embodied:in the form of a diaphragm, including a ceramic diaphragm, a fiber diaphragm or a ground diaphragm;in the form of an annular gap;in the form of an organic or inorganic membrane;in the form of a gel; orin the form of a dispersion.5. The protective device of claim 1 , ...

METHODS AND SYSTEMS FOR MEASURING ANIONS

Embodiments of the present disclosure provide for methods for detecting the presence and/or concentration of anions in a solution, systems for detecting the presence and/or concentration of anions in a solution, anion sensor systems, and the like. 1. A method of detecting anions , comprising:contacting a solution including an anion with a Pt(II) porphyrin;irradiating the solution with visible light;measuring a photoluminescence signal from the Pt(II) porphyrin; anddetermining the concentration of the anion;wherein the Pt(II) porphyrin is 5, 10, 15, 20-tetra(1-methyl-4-pyridino)-porphyrin Pt(II) tetrachloride, an analogue thereof, or a derivative thereof.2. The method of claim 1 , wherein the anion is selected from the group consisting of: a halogen anion claim 1 , a sulfide anion claim 1 , and a cyanide anion.3. The method of claim 1 , wherein the anion is iodide.4. The method of claim 1 , wherein the anion is present at a level of at least about 30 pmol in the solution.5. The method of claim 1 , further comprising: measuring a UV-Vis signal.6. A sensor system claim 1 , comprising:a structure having a Pt(II) porphyrin disposed on the surface;a compartment including a solution including an anion, wherein the anion and Pt(II) porphyrin interact to quench the photoluminescence of the Pt(II) porphyrin once the structure is placed in the compartment with the solution;a system for irradiating the solution with visible light; anda system for measuring a signal selected from a photoluminescence signal, UV-Vis signal, or a combination thereof, wherein a change in the signal relative to a signal without the anion present is correlated to the presence of the anion in the solution.7. The system of claim 6 , wherein the anion is selected from the group consisting of a halogen anion claim 6 , a sulfide anion claim 6 , and a cyanide anion.8. The system of claim 6 , wherein the anion is iodide.9. The system of claim 6 , wherein the anion is present at a level of at least about 30 ...

SELECTIVE DELIVERY MOLECULES AND METHODS OF USE

Disclosed herein is a selective delivery molecule comprising: (a) an acidic sequence (portion A) which is effective to inhibit or prevent the uptake into cells or tissue retention, (b) a molecular transport or retention sequence (portion B), and (c) a linker between portion A and portion B, and (d) at least one cargo moiety. 2. The molecule of claim 1 , wherein A and B do not have an equal number of acidic and basic amino acids.3. The molecule of claim 2 , wherein the number of basic amino acids in B is greater than the number of acidic amino acids in A.4. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates.5. The molecule of claim 1 , wherein B is a peptide comprising 8 or 9 consecutive arginines.6. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates and B is a peptide comprising 8 or 9 consecutive arginines.7. The molecule of claim 6 , wherein A is a peptide comprising 5 consecutive glutamates and B is a peptide comprising 8 consecutive arginines.8. The molecule of claim 1 , wherein c claim 1 , c claim 1 , and care each independently selected from a naturally-occurring amino acid or a non-naturally-occurring amino acid.9. The molecule of claim 8 , wherein c claim 8 , c claim 8 , and care each independently selected from a D amino acid claim 8 , a L amino acid claim 8 , an α-amino acid claim 8 , a β-amino acid claim 8 , or a -amino acid.10. The molecule of claim 1 , wherein c claim 1 , c claim 1 , and care each independently selected from any amino acid having a free thiol group claim 1 , any amino acid having a N-terminal amine group claim 1 , and any amino acid with a side chain capable of forming an oxime or hydrazone bond upon reaction with a hydroxylamine or hydrazine group.11. The molecule of claim 1 , wherein c claim 1 , c claim 1 , and care each independently selected from D-cysteine claim 1 , D-glutamate claim 1 , lysine claim 1 , and para-4-acetyl L-phenylalanine.12. The molecule ...

METHOD FOR DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE USED THEREIN

Disclosed herein is a nucleic acid probe for detecting a target nucleic acid. At least one terminal of the probe-binding region in the target nucleic acid is a guanine base, and one or more cytosine bases are present within 1 to 7 bases from the guanine base. The nucleic acid probe comprises an oligonucleotide having a cytosine base facing the guanine base on a terminal and a fluorescent dye conjugated to the cytosine base. The fluorescent dye is quenched by the interaction with a guanine base. The oligonucleotide is completely complementary to the nucleic acid in the probe-binding region except the one or more cytosine bases present within 1 to 7 bases from the terminal guanine base. The base in the oligonucleotide facing the cytosine base closest to the terminal guanine base among the one or more cytosine bases is a base having no fluorescence-quenching effect. 1. A first nucleic acid probe for detecting a target nucleic acid , whereinthe target nucleic acid comprises a probe-binding region with which the first nucleic acid probe hybridizes;at least one terminal of the probe-binding region is a guanine base, and one or more cytosine bases are present within 1 to 7 bases from the guanine base in the probe-binding region;the first nucleic acid probe comprises an oligonucleotide having a cytosine base facing t guanine base on a terminal and a fluorescent dye conjugated to the cytosine base;the fluorescent dye is a fluorescent dye that is quenched by the interaction with a guanine base; andthe oligonucleotide is complementary to the nucleic acid in the probe-binding region except the cytosine base present within 1 to 7 bases from the terminal guanine base, and one or more bases in the oligonucleotide facing the one or more cytosine bases are a guanine base or a base having no fluorescence-quenching effect, provided that the base in the oligonucleotide facing the cytosine base closest to the terminal guanine base among the one or more cytosine bases is a base having no ...

CORE-SHELL NANOFIBER-BASED SENSORS

Nanofiber-based sensors for the rapid detection, identification, and/or quantification of analytes, including gaseous analytes such as oxygen, are provided. The nanofiber-based sensors can comprise core-shell nanofibers. The core-shell nanofibers can comprise (a) a core comprising a first polymer and an sensor dispersed therein; and (b) a shell disposed coaxially around the core, comprising a second polymer. 1. A core shell nanofiber comprising (a) a core comprising a first polymer and an oxygen sensor dispersed therein; and (b) a shell comprising a second polymer disposed coaxially around the core.2. The nanofiber of claim 1 , wherein the first polymer is oxygen permeable.3. The nanofiber of claim 2 , wherein the first polymer has an oxygen diffusivity greater than water.4. The nanofiber of claim 1 , wherein the nanofibers are substantially resistant to photobleaching and heat.5. The nanofiber of claim 1 , wherein the second polymer is biocompatible.6. The nanofiber of claim 1 , wherein the first polymer is selected from the group consisting of polyethersulfone and polydimethylsiloxane.7. The nanofiber of claim 1 , wherein the second polymer is polycaprolactone.8. The nanofiber of claim 1 , wherein the oxygen sensor comprises a luminophore.9. The nanofiber of claim 8 , wherein the luminophore exhibits an emission peak of from about 570 nm to about 670 nm.10. The nanofiber of claim 8 , wherein the oxygen sensor is selected from the group consisting of tris (4 claim 8 ,7-diphenyl-1 claim 8 ,10-phenanthroliine) ruthenium (II) dichloride claim 8 , (4 claim 8 ,7-diphenyl-1 claim 8 ,10-phenanthroliine) ruthenium (II) tetraphenylboron claim 8 , platinum (II) octaethylporphinedialkylcarbocyanine claim 8 , and diocadecylcycloxacarbocyanine.11. The nanofiber of claim 1 , wherein the diameter of the nanofiber is selected from the group consisting of between about 400 nm to about 600 nm; about 640 nm to about 1280 nm; about 317 nm to about 707 nm; about 270 nm to about 532 nm; ...

NANOSENSOR FOR ASSESSING THROMBIN INHIBITORS

Constructs and monitoring systems to assess the level of molecules that bind to thrombin (e.g. thrombin regulators and inhibitors) are provided. The constructs are nanosensors comprising i) a thrombin molecule to which is bound a reporter ligand comprising a fluorescent label, ii) a fluorescence-quenching metal nanoparticle, and, optionally iii) a fluorescence-quenching dye molecule attached to one or both of the nanoparticle and the thrombin molecule. The binding of a thrombin regulator or inhibitor to the thrombin molecule displaces the reporter ligand, and the signal from the fluorescent label increases. The increase is proportional to the concentration of thrombin-binding molecule in the sample. 1. A nanoprobe comprising:a metal nanoparticle;a thrombin molecule attached to said metal nanoparticle, said thrombin molecule comprising at least one ligand binding site; anda reporter ligand non-covalently bound to said at least one ligand binding site, said reporter ligand comprising a fluorescent label;wherein an absorbance spectrum of said metal nanoparticle overlaps an emission spectrum of said fluorescent label;and wherein a distance between said metal nanoparticle and said fluorescent label allows quenching of a fluorescence signal from said fluorescent label by said metal nanoparticle.2. The nanoprobe of claim 1 , wherein said thrombin molecule is covalently attached to said metal nanoparticle via a first linker molecule.4. The nanoprobe of claim 1 , wherein said reporter ligand comprises hirudin or a thrombin-binding portion of hirudin.5. The nanoprobe of claim 1 , wherein said fluorescent label is selected from the group consisting of fluorescein claim 1 , cyanine claim 1 , tetramethylrhodamine claim 1 , and boron-dipyrromethene (BODIPY®).6. The nanoprobe of claim 1 , wherein said metal nanoparticle is selected from the group consisting of gold claim 1 , iron claim 1 , copper claim 1 , silver claim 1 , platinum claim 1 , tungsten and alloys and derivatives ...

FLUORESCENT SILANE LAYERS FOR DETECTING EXPLOSIVES

Detection reagent for an analyte comprising an NOgroup, wherein the detection reagent comprises an arylamine, and a structural formula of the arylamine is selected from the structural formulae 1, 2 and 3: 2. original) Detection reagent according to claim 1 , wherein R claim 1 , R claim 1 , Rand Rare H.3. Detection reagent according to claim 1 , wherein Ris a phenyl group and the arylamine thus comprises a triphenylamine motif.4. Detection reagent according to claim 3 , wherein the triphenylamine motif is joined covalently to a phenyl group in at least one para position claim 3 , and the remaining para positions are unsubstituted or methylated.5. Detection reagent according to claim 4 , wherein the triphenylamine motif and the phenyl group are joined via a triple bond claim 4 , via a double bond or via a single bond.7. Detection reagent according to claim 6 , wherein the analyte comprising the NOgroup is selected from: TNT claim 6 , DNT claim 6 , tetryl claim 6 , PETN claim 6 , NG claim 6 , EGDN claim 6 , DNDMB claim 6 , ammonium nitrate claim 6 , RDX and HMX.8. Detection reagent according to claim 1 , wherein the analyte comprising the NOx group is present in a sample comprising an organic solution claim 1 , an aqueous solution claim 1 , a mixed organic/aqueous solution claim 1 , an air sample and/or a wiped sample.9. Detection reagent according to claim 1 , wherein Rand Rin the detection reagent are selected from COX and PhCOX with X=4-iodophenyl;4-bromophenyl; 4-chlorophenyl; 4-vinylphenyl or 4-allylphenyl and the detection reagent, after a reaction with a reactive organosilane by means of Heck or metathesis reaction, is covalently bonded to a substrate and/or forms a monomolecular layer over at least parts of the substrate.10. Detection reagent according to claim 1 , wherein Rand Rin the detection reagent are selected from COY and PhCOY with Y=2-methyl-3-pentyn-2-yl or 3-tert-butyl-4 claim 1 ,4-dimethyl-1-pentyn-3-yl and the detection reagent has been adsorbed on ...

SYSTEMS AND METHODS FOR CYCLIC FLUORESCENCE IMAGING

Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner. 1. A method of labeling and de-labeling a cell , the method comprising:providing a cell associated with a substrate;contacting the cell with a detectable agent;labeling a plurality of sites of the cell with the detectable agent;applying a voltage across the cell; andde-labeling the cell, wherein de-labeling comprises removal of or quenching of the detectable agent on the cell.2. A method of labeling and de-labeling a cell , the method comprising:providing a cell associated with a substrate;contacting the cell with a detectable agent;labeling a plurality of sites of the cell with the detectable agent;applying a voltage to a solution in contact with the cell; andde-labeling the cell.3. The method of or , wherein applying voltage to a solution in contact with the cell generates one or more reactive chemical species.4. The method of or , wherein de-labeling the cell comprises contacting the detectable agent with the one or more reactive chemical species.5. The method of or , further comprising detecting the detectable agent after labeling the plurality of sites.6. The method of any one of - , wherein the detecting comprises optically detecting the detectable agent.7. The method of any one of - , wherein the labeling and de-labeling is performed in less than 20 minutes , less than 15 minutes , less than 10 minutes , less than 9 minutes , less than 8 minutes , less than 7 minutes , less than 6 minutes , less than 5 minutes , less than 4 minutes , or less than 3 minutes.8. The method of any one of - , wherein the method is repeated for at least 2 times , at least 3 times , at least 4 times , at least 5 times , at least 10 times , or at least 50 times.9. The method of any one of - , wherein the method is ...

POLYMERASE COMPOSITIONS & METHODS

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein. 1. A DNA polymerase having a photostability that is at least about 80% under standard photostability assay conditions.2. The DNA polymerase of claim 1 , wherein the polymerase comprises an amino acid sequence that is at least about 95% identical to the amino acid sequence of SEQ ID NO: 7.3. The DNA polymerase of claim 2 , further comprising one or more amino acid substitutions selected from the group consisting of: D9A claim 2 , E11A claim 2 , E11I claim 2 , T12I claim 2 , H58R claim 2 , N59D claim 2 , D63A claim 2 , Y162F claim 2 , Y162C claim 2 , D166A claim 2 , Q377A claim 2 , S385G claim 2 , H370G claim 2 , H370T claim 2 , H370S claim 2 , H370K claim 2 , H370R claim 2 , H370A claim 2 , H370Q claim 2 , H370W claim 2 , H370Y claim 2 , H370F claim 2 , E371G claim 2 , E371H claim 2 , E371T claim 2 , E371S claim 2 , E371K claim 2 , E371R claim 2 , E371A claim 2 , E371Q claim 2 , E371W claim 2 , E371Y claim 2 , E371F claim 2 , K372G claim 2 , K372E claim 2 , K372T claim 2 , K372S claim 2 , K372R claim 2 , K372A claim 2 , K372Q claim 2 , K372W claim 2 , K372Y claim 2 , K372F claim 2 , K380E claim 2 , K380T claim 2 , K380S claim 2 , K380R claim 2 , K380A claim 2 , K380Q claim 2 , K380W claim 2 , K380Y claim 2 , K380F claim 2 , D507H claim 2 , D507G claim 2 , D507E claim 2 , D507T claim 2 , D507S claim 2 , D507R claim 2 , D507A claim 2 , D507R claim 2 , D507Q ...

METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule. 1. A method for generating an energy transfer signal comprising the steps of: contacting (i) a polymerase having altered nucleotide incorporation kinetics and linked to an energy transfer donor moiety with (ii) a nucleic acid molecule and with (iii) at least one type of a nucleotide having an energy transfer acceptor moiety , so as to incorporate the nucleotide into the nucleic acid molecule thereby locating the polymerase and nucleotide in close proximity with each other to generate the energy transfer signal.2. A method for generating an energy transfer signal comprising the steps of: contacting (i) a polymerase having altered nucleotide incorporation kinetics and linked to an energy transfer donor moiety with (ii) a nucleic acid molecule and with (iii) at least one type of a hexaphosphate nucleotide having an energy transfer acceptor moiety , so as to incorporate the hexaphosphate nucleotide into the nucleic acid molecule thereby locating the polymerase and nucleotide in close proximity with each other to generate the energy transfer signal.3. A method for generating an energy transfer signal comprising the steps of: ...

CONJUGATES OF BIOMOLECULES TO NANOPARTICLES

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing. 1. A composition , comprising a polymerase-nanoparticle conjugate including a polymerase linked to a nanoparticle , wherein the conjugate has polymerase activity.2. The composition of claim 1 , wherein the polymerase activity of the conjugate is at least about 1% relative to the polymerase activity of the unconjugated polymerase.3. The composition of claim 2 , wherein the polymerase activity is at least about 70% relative to the polymerase activity of the unconjugated polymerase.4. The composition of claim 1 , wherein the polymerase comprises a His tag.5. The composition of claim 1 , wherein the polymerase comprises one member of a binding pair and the nanoparticle comprises a complementary member of the binding pair.6. The composition of claim 1 , wherein the polymerase is a DNA polymerase.7E. coli. The composition of claim 6 , wherein the DNA polymerase is at least 95% identical to a DNA polymerase selected from the group consisting of: Phi-29 DNA polymerase claim 6 , B103 DNA polymerase claim 6 , the Klenow fragment of DNA polymerase and HIV reverse transcriptase.8. The composition of claim 1 , wherein the polymerase link is selected from one of a group consisting of: covalent bonding claim 1 , affinity bonding and electrostatic bonding.9. The composition of ...

FLUORESCENCE QUENCHING IMMUNOASSAY

The present invention relates to a method and reagents for determining the presence of or the amount of an analyte in a sample. 2. The method of claim 1 , wherein the analyte is a macromolecule.3. The method of claim 2 , wherein the analyte is a protein.4. The method of claim 3 , wherein (i) the fluorescent tracer is a fluorescent moiety attached to an amino acid chain claim 3 , wherein the length of the amino acid chain is shorter than the amino acid length of the protein claim 3 , and wherein the amino acid sequence of the amino acid chain comprises the amino acid sequence of an epitope on the protein that is responsible for complexing with the binding partner claim 3 , and (ii) the binding partner is optionally conjugated to a quencher.5. The method of claim 4 , wherein the quencher is selected from the group consisting of CY3 claim 4 , CY5 claim 4 , IRDyeQC1 claim 4 , BHQ1 claim 4 , and BHQ10.6. The method of claim 4 , wherein the binding partner is an antibody.7. The method of claim 4 , wherein the protein is selected from the group consisting of canine CysB protein and canine NT-proBNP.8. The method of claim 7 , wherein the protein is canine CysB protein.9. The method of claim 8 , wherein the binding partner is an anti-cystatin-B antibody and the fluorescent tracer is fluorescein attached to an amino acid sequence selected from the group consisting of SEQ ID NO: 2 claim 8 , SEQ ID NO: 3 claim 8 , SEQ ID NO: 4 claim 8 , SEQ ID NO: 5 claim 8 , SEQ ID NO: 6 claim 8 , and SEQ ID NO: 7.10. The method of claim 9 , wherein the amino acid sequence is attached to the 5-position of the fluorescein.11. The method of claim 10 , wherein the amino acid sequence is attached to the 5-position of the fluorescein with a —NH—C(O)— linker.12. The method of claim 10 , wherein the anti-cystatin-B antibody is conjugated to a quencher.13. The method of claim 9 , wherein the amino acid sequence is attached to the 4′-position of the fluorescein.14. The method of claim 13 , wherein the ...

DUPLEX STABILIZING FLUORESCENCE QUENCHERS FOR NUCLEIC ACID PROBES

Diaryl-azo derivatives are efficient fluorescence quenchers as well as nucleic acid duplex-stabilizing agents and are useful in oligonucleotide conjugates and probes. The oligonucleotide-quencher conjugates may be used in detection methods for nucleic acid targets. 2. The quencher reagent of claim 1 , wherein Arand Arare substituted with a reactive group claim 1 , a linker with a functional group or a protected functional group claim 1 , or a linker connected to a synthesis solid support.3. A method for preparing an oligonucleotide conjugate labeled with a quencher claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'reacting an oligonucleotide with the quencher reagent of to produce an oligonucleotide conjugate labeled with a quencher.'}5. A method for preparing an oligonucleotide conjugate labeled with a quencher claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'reacting an oligonucleotide with the quencher reagent of to produce an oligonucleotide conjugate labeled with a quencher.'}7. The quencher reagent of claim 6 , wherein the exocyclic amino group is substituted with one or two of an alkyl claim 6 , a linker with a functional group or protected functional group claim 6 , or a linker connected to a synthesis solid support.8. A method for preparing an oligonucleotide conjugate labeled with a quencher claim 6 , comprising:{'claim-ref': {'@idref': 'CLM-00006', 'claim 6'}, 'reacting an oligonucleotide with the quencher reagent of to produce an oligonucleotide conjugate labeled with a quencher.'}10. A method for preparing an oligonucleotide conjugate labeled with a quencher claim 6 , comprising:{'claim-ref': {'@idref': 'CLM-00009', 'claim 9'}, 'reacting an oligonucleotide with the quencher reagent of to produce an oligonucleotide conjugate labeled with a quencher.'}12. The oligonucleotide conjugate of claim 11 , wherein the oligonucleotide further comprises a fluorophore connected to the oligonucleotide.13. The ...

Fluorescent probe

A fluorescent probe is obtained via hydrolysis and condensation reaction using 3-glycidoxypropyl trimethoxysilane. The fluorescent probe includes a silicon oxide core and a self-assembled monolayer. The self-assembled monolayer has an epoxide group, and joins the silicone oxide core by a covalent bond. The epoxide group of the fluorescent probe can form a conjugated bond with a molecule with an amino group via an aminolysis reaction, forming a nanoparticle including the molecule and the fluorescent probe.

DNA-based molecular switches and uses thereof

Disclosed are nucleic acid-based molecular switches that respond to changes in pH. The switches may be used in DNA nanodevices. The switches may also act as sensors for measuring the pH of a sample, including cells, regions thereof, and whole organisms. The switch includes an A-motif that forms at acidic pH. Also disclosed are compositions and methods for measuring the pH of cells or regions thereof, such as vesicles, the nucleus, mitochondrial matrix, or the Golgi lumen. 1. A method for determining the pH of a sample , the method comprising:contacting the sample with one or more indicators having at least two poly dA nucleic acids comprising a signaling system, wherein the one or more indicators transition between first and second stable conformations in response to a change in pH; anddetecting the presence, absence, or magnitude of a signal from the signaling system to determine the pH of the sample.2. The method of claim 1 , wherein the detecting comprises measuring the magnitude of the signal generated claim 1 , wherein the magnitude indicates the pH of the sample.3. The method of claim 1 , wherein the magnitude of the signal changes as the pH varies from pH 2 to 10.4. The method of claim 1 , wherein the magnitude of the signal changes as the pH varies from pH 5 to 7.5. The method of claim 1 , wherein the signaling system comprises an interacting label pair.6. The method of claim 5 , wherein the interacting label pair comprises a fluorophore and quencher pair.7. The method of claim 6 , wherein the fluorophore and the quencher are on separate poly dA nucleic acids claim 6 , such that a difference in a fluorescent signal is detectable upon a change in conformation of the one or more indicators.8. A method for determining the pH of a cell or region thereof claim 6 , the method comprising:contacting the cell with one or more indicators having at least two poly dA nucleic acids comprising a signaling system, wherein the one or more indicators transition between first ...

MEASURING ARRANGEMENT FOR DETERMINING AN OZONE CONTENT OF A MEASURED MEDIUM

The present disclosure relates to a measuring arrangement for measuring an ozone content in a measured medium, including: a first sensor surface and a second sensor surface; a first cover element adjacent the first sensor surface and including an ozone binder that binds ozone without releasing oxygen or any species further reacting to form oxygen; a second cover element adjacent the second sensor surface and including an ozone converter that reacts with ozone to form oxygen; a measuring sensor configured to generate a first measurement signal dependent on the oxygen concentration at the first sensor surface and a second measurement signal dependent on the oxygen concentration at the second sensor surface; and an electronic evaluation unit configured to determine the ozone content in the measured medium based on the first and the second measurement signals. 1. A measuring arrangement for measuring an ozone content in a measured medium , the measuring arrangement comprising:a first sensor surface and a second sensor surface;a first cover element adjacent the first sensor surface and including an ozone binder that binds ozone without releasing oxygen or any species further reacting to form oxygen, wherein a side of the first cover element opposite the first sensor surface is adapted to contact the measured medium, and wherein the first cover element is permeable at least to oxygen;a second cover element adjacent the second sensor surface and including an ozone converter that reacts with ozone to form oxygen, wherein a side of the second cover element opposite the second sensor surface is adapted to contact the measured medium, and wherein the second cover element is permeable at least to oxygen;a measuring sensor configured to generate a first measurement signal dependent on the oxygen concentration present at the first sensor surface and a second measurement signal dependent on the oxygen concentration present at the second sensor surface; andan electronic evaluation ...

Protein L based bioassay method for determining presence of soluble antibodies in a sample and kit therefor

This invention relates to bioassay method wherein the presence of a specific soluble antibody in a sample of a bodily fluid, preferably plasma or serum, of an animal, including human, is qualitatively and/or quantitatively determined. The method employs, a first group labelled with an energy donor and a second group labelled with an energy acceptor; the first group, or second group, comprising an antigen of the soluble antibody and the second group, or first group, respectively, comprising a Fab binding moiety capable of binding to a Fab region of antibodies of said animal. The donor and the acceptor form an energy transfer pair capable of energy transfer. The invention further comprises a kit for the bioassay method. 1. A bioassay method wherein the presence of a specific soluble antibody of an animal , including human , in a sample comprising a bodily fluid of said animal is qualitatively and/or quantitatively determined , said method employinga) a first group including an energy donor, andb) a second group including an energy acceptor or quencher, whereini) said first group or said second group comprises an antigen of said soluble antibody, andii) said second group or said first group, respectively, comprises a fragment antigen-binding (Fab) binding moiety (FBM) coupled to said energy donor, or said energy acceptor, including quencher, respectively, said moiety being capable of binding to a Fab region of antibodies of said animal,wherein said energy donor and said energy acceptor or quencher form an energy transfer pair capable of energy transfer; andthe FBM is selected from the group consisting of protein L, recombinant protein L, Ch1 binding protein, and functional domains, fragments and combinations thereof;preferably recombinant protein L and functional domains, fragments and combinations thereof.2. The bioassay method according to wherein the soluble antibody is a human soluble antibody.3. The bioassay method according to wherein the soluble antibody is an ...

BETA-LACTAMASE TARGETED PHOTOSENSITIZER FOR PESTICIDE AND PEST DETECTION

Photoactivatable pesticide compounds and methods for the use thereof in the elimination and detection of pests are provided. 1. A pesticidal composition comprising a pesticidally effective amount of one or more photosensitizers that are linked by one or more moieties cleavable by a β-lactamase expressed by a pest wherein said linked moieties are present in an amount sufficient to quench photoactivation of said photosensitizers and wherein said one or more photosenitizers are capable of generating a phototoxic species upon dequenching and light-activation.2. The pesticidal composition of claim 1 , wherein the one or more moieties cleavable by the β-lactamase comprise: a cephalosporin claim 1 , a penicillin claim 1 , a penem claim 1 , a carbapenem claim 1 , a monocyclic mobactem claim 1 , or a fragment thereof.3. The photosensitizer composition of claim 2 , wherein the one or more moieties cleavable by the β-lactamase comprises a cephalosporin claim 2 , a penicillin claim 2 , or a fragment thereof.4. The pesticidal composition of claim 3 , wherein the cephalosporin or penicillin fragment comprises a beta-lactam ring.5. The pesticidal composition of claim 3 , wherein the one or more moieties cleavable by β-lactamase is a cephalosporin.6. The pesticidal composition of claim 5 , wherein at least one photosensitizer is bound at the 3′ position of a cephalosporin.7. The pesticidal composition of claim 1 , wherein the photosensitizer is a porphyrin.8. The pesticidal composition of claim 7 , wherein the porphyrin is selected from the group consisting of a porfimer sodium claim 7 , hematoporphyrin IX claim 7 , hematoporphyrin ester claim 7 , dihematoporphyrin ester claim 7 , synthetic diporphyrin claim 7 , O-substituted tetraphenyl porphyrin claim 7 , 3 claim 7 ,1-meso tetrakis porphyrin claim 7 , hydroporphyrin claim 7 , benzoporphyrin derivative claim 7 , benzoporphyrin monoacid derivative claim 7 , monoacid ring derivative claim 7 , tetracyanoethylene adduct of ...

NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE

Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems. 1. An isolated polynucleotide encoding a click beetle red luciferase (CBR) variant polypeptide having at least 80% amino acid sequence identity to SEQ ID NO: 1 and comprising at least one amino acid substitution at a position corresponding to position 4 , 16 , 34 , 47 , 51 , 52 , 55 , 72 , 73 , 74 , 79 , 82 , 83 , 87 , 89 , 104 , 109 , 113 , 117 , 119 , 124 , 130 , 131 , 133 , 136 , 144 , 146 , 156 , 159 , 170 , 179 , 186 , 200 , 211 , 218 , 224 , 225 , 226 , 228 , 229 , 234 , 247 , 251 , 252 , 253 , 255 , 280 , 281 , 285 , 308 , 309 , 310 , 319 , 329 , 334 , 335 , 337 , 346 , 348 , 349 , 350 , 352 , 354 , 355 , 358 , 363 , 370 , 377 , 390 , 393 , 394 , 400 , 401 , 409 , 412 , 420 , 422 , 431 , 437 , 439 , 444 , 445 , 453 , 455 , 467 , 471 , 473 , 479 , 484 , 489 , 496 , 501 , 503 , 508 , 516 , 528 , 531 , 535 , 537 , 539 , or combination thereof , of SEQ ID NO: 1 , wherein the variant CBR polypeptide has at least one of enhanced luminescence , altered light emission wavelength , altered substrate specificity , or a combination thereof , as compared to a CBR polypeptide of SEQ ID NO: 1.2. The isolated polynucleotide of claim 1 , wherein the CBR variant polypeptide further comprises at least one amino acid substitution at a position corresponding to position 351 claim 1 , 389 claim 1 , 457 claim 1 , or combination thereof claim 1 , of SEQ ID NO: 1.3. The isolated polynucleotide of claim 2 , wherein the CBR variant polypeptide comprises a substitution corresponding to at least one of R4H claim 2 , H16Q claim 2 , H34Y claim 2 , ...

PROBES AND ASSAYS FOR FLUORESCENT IN-SITU HYBRIDIZATION IMAGING USING MULTIPLEXED FLUORESCENT SWITCHING

A readout probe for use in fluorescent in-situ hybridization imaging has a targeting portion to bind to a first hybridization sequence in an encoding probe that targets an analyte in a sample, a first fluorophore to emit light at a wavelength range, and a first cleaving region between the first fluorophore and the targeting portion. 1. A readout probe for use in fluorescent in-situ hybridization imaging , the readout probe comprising:a targeting portion to bind to a first hybridization sequence in an encoding probe that targets an analyte in a sample;a first fluorophore to emit light at a wavelength range; anda first cleaving region between the first fluorophore and the targeting portion.2. The readout probe of claim 1 , wherein the first cleaving region comprises a photocleavable region.3. The readout probe of claim 1 , wherein the first cleaving region comprises an enzymatically cleavable region.4. The readout probe of claim 1 , wherein the first cleaving region comprises a chemically cleavable region.5. The readout probe of claim 1 , further comprising a second fluorophore to emit light at a different wavelength range or in response to a different excitation wavelength.6. The readout probe of claim 5 , comprising a second cleaving region between the second fluorophore and the targeting portion claim 5 , the second cleaving region cleavable in response to a different cleaving process than the first cleaving region.7. The readout probe of claim 5 , wherein the second fluorophore is coupled to the targeting region without a cleavable region.8. The readout probe of claim 5 , comprising a quencher coupled to the second fluorophore to suppress excitation of the second fluorophore claim 5 , and a second cleaving region between the second fluorophore and the quencher.9. A readout probe for use in fluorescent in-situ hybridization imaging claim 5 , the readout probe comprising:a targeting portion to bind to a hybridization sequence in an encoding probe that targets an ...

LUCIFERASE-BASED THERMAL SHIFT ASSAYS

Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate). 1. A system comprising:(a) a fusion of a target protein and a bioluminescent reporter, wherein the bioluminescent reporter protein has a emission spectra that encompasses a wavelength X; and (1) binds nonspecifically to aggregated proteins and/or hydrophobic peptide segments, and', '(2) has an excitation spectra that encompasses the wavelength X., '(b) a fluorescent dye that2. The system of claim 1 , wherein the fluorescent dye is quenched by water.3. The system of claim 1 , wherein the fluorescent dye is fluorogenic.4. The system of claim 1 , wherein the emission spectra of the bioluminescent reporter protein and the excitation spectra of the fluorescent dye overlap such that emission from the bioluminescent reporter excites the fluorescent dye by BRET.5. The system of claim 1 , wherein the system is a cell claim 1 , cell lysate claim 1 , or reaction mixture.6. The system of claim 1 , wherein the bioluminescent reporter is a luciferase.7Oplophorus gracilirostris. The system of claim 6 , wherein the luciferase is a variant luciferase (OgLuc).8. The system of claim 1 , wherein the bioluminescent reporter is a peptide or polypeptide tag that forms a bioluminescent complex upon interaction with a complement polypeptide or peptide.9. The system of claim 8 , further comprising complement polypeptide or peptide.10. The system of claim 1 , wherein the fluorescent dye is SYPRO Orange claim 1 , SYPRO Red claim 1 , Nile Red claim 1 , ANS claim 1 , Bis-ANS claim 1 , SYPRO Ruby claim 1 , SYPRO Tangerine claim 1 , and/or Dapoxyl Sulfonic Acid Sodium Salt claim 1 , Protein Thermal Shift™ Dye (Life Technologies) claim 1 , Proteostat™ Dye (Enzo) claim 1 , or combinations thereof.11. The system of claim 1 , further comprising a ligand of the target or ...

AUTOMATED ANALYSIS TOOL FOR BIOLOGICAL SPECIMENS

Systems and methods are described for analyzing a plurality of biological samples with a plurality of fluorescent reagents in an automated fashion. The system may include two optical systems, a fluorescence imaging system and an optical quenching system. These two systems may be placed laterally adjacent to one another, and generally beneath one of the wells of sample-containing microtiter plate. The biological sample contained therein may be stained with a series of fluorescent reagents, with the fluorescence quenched between stainings. By precise positioning of the sample with respect to the imaging system, the sample may be imaged with a plurality of serially applied reagents. 1. An automated system for analyzing biological samples stained with at least one fluorescent dye , comprising:a stage movable laterally in the x- and y-direction for holding at least one biological sample stained with the fluorescent dye;a detector for detecting the fluorescence signals obtained from the fluorescent dye on the biological sample in a field of view of the detector, anda fluorescence system including a light source producing a first radiation which excites the fluorescent dye on the biological sample by impinging its radiation on the field of view,a controller executing a routine that moves the biological sample by shifting the biological sample laterally on the movable stage to the field of view of the detector;and wherein the controller directs the light source to produce a second radiation which quenches the fluorescence signals obtained from at least a part of the sample, after detection by the detector.2. The automated system of claim 1 , further comprising a fluid handling system providing at least one of fluorescence dyes claim 1 , compounds quenching the fluorescence signals claim 1 , and washing fluids and buffer to the biological sample.3. The automated system of claim 1 , wherein the controller collects the fluorescence signals from the detector as images of the ...

MULTIPLEX Q-PCR ARRAYS

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. 147.-. (canceled)48. A system comprising:a polymerase chain reaction (PCR) amplification reaction chamber capable of receiving: (i) a substrate comprising a surface with an array of nucleic acid probes at independently addressable locations, and (ii) a fluid to be held in contact with the substrate, the fluid comprising a nucleic acid sample comprising multiple nucleotide sequences, primers, and enzymes;a temperature controller capable of carrying out multiple PCR temperature phases and temperature cycles comprising: (i) a heating and cooling module for raising and lowering the temperature of the fluid and/or the substrate; and (ii) a temperature sensor; anda detector capable of detecting light signals as a function of time from the independently addressable locations on the substrate within the chamber at a specific phase or phases during or after a plurality of temperature cycles while the fluid is in contact with the substrate.49. The system of further comprising:an analysis block comprising a computer and software capable of determining the amounts of amplified products hybridized to the array of probes using the detected light as a function of time, and of determining the amounts of multiple nucleotide sequences in a sample using the amounts of amplified products determined during or after a plurality of temperature cycles.50. The system of wherein the detector comprises a ...

OLIGONUCLEOTIDE-BASED PROBES AND METHODS FOR DETECTION OF MICROBES

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays. 1. (canceled)2. A method of detecting endonuclease activity in a test a sample , comprising:(a) contacting the test sample with a probe for detecting a microbial endonuclease comprising an oligonucleotide, a fluorophore operably linked to the oligonucleotide, and a quencher operably linked to the oligonucleotide, wherein the oligonucleotide comprises one or more modified nucleotides, is capable of being cleaved by a microbial nuclease, and has a DNA TT di-nucleotide, DNA AT di-nucleotide, DNA AA di-nucleotide or DNA TA di-nucleotide to form a digested probe, and(b) measuring the fluorescence emitted by the digested probe.3. The method of claim 2 , wherein the at least one fluorophore is selected from the group consisting of the fluorophores Hydroxycoumarin claim 2 , Alexa fluor claim 2 , Aminocoumarin claim 2 , Methoxycoumarin claim 2 , Cascade Blue claim 2 , Pacific Blue claim 2 , Pacific Orange claim 2 , Lucifer yellow claim 2 , Alexa fluor 430 claim 2 , NBD claim 2 , R-Phycoerythrin (PE) claim 2 , PE-Cy5 conjugates claim 2 , PE-Cy7 conjugates claim 2 , Red 613 claim 2 , PerCP claim 2 , Cy2 claim 2 , TruRed claim 2 , FluorX claim 2 , Fluorescein claim 2 , FAM claim 2 , BODIPY-FL claim 2 , TET claim 2 , Alexa fluor 532 claim 2 , HEX claim 2 , TRITC claim 2 , Cy3 claim 2 , TMR claim 2 , Alexa fluor 546 claim 2 , Alexa fluor 555 claim 2 , Tamara claim 2 , X-Rhodamine claim 2 , Lissamine Rhodamine B claim 2 , ROX claim 2 , Alexa fluor 568 claim 2 , Cy3.5 581 claim 2 , Texas Red claim 2 , Alexa fluor 594 claim 2 , Alexa fluor 633 claim 2 , LC red 640 claim 2 , Allophycocyanin (APC) claim 2 , Alexa fluor 633 claim 2 , APC-Cy7 conjugates claim 2 , Cy5 claim 2 , Alexa fluor 660 claim 2 , Cy5.5 claim 2 , LC red 705 claim 2 , Alexa fluor 680 claim 2 , Cy7 claim ...

METHODS AND DEVICES FOR MONITORING BLOOD

A system includes a cuvette including a cuvette body forming a substantially planar exterior surface and having a sensor window defined within the substantially planar exterior surface. The cuvette further includes a probe retention structure extending from the cuvette body. The system includes a probe with a probe body and a protrusion that is removably coupled to the probe retention structure. 1. A cuvette comprising:a cuvette body having a first end, a second end, an aperture positioned between the first end and the second end, and a central bore extending from the first end to the second end to permit blood flow therethrough; anda sensor window positioned within the aperture of the cuvette body, the sensor window providing visual and/or sensor access to blood within the central bore;wherein the sensor window includes a fluorescent coating sensitive to blood oxygen partial pressures.2. The cuvette of claim 1 , further comprising a sensor window frame extending around the aperture claim 1 , the sensor window disposed in the sensor window frame.3. The cuvette of claim 2 , further comprising a window retainer mechanically coupling the sensor window to the cuvette body4. The cuvette of claim 3 , wherein the cuvette body forms at least two teeth mechanically coupled to the window retainer.5. The cuvette of claim 3 , wherein the window retainer is U-shaped and defines a circular aperture formed in a surface of the window retainer configured to be aligned with the sensor window to permit optical and/or sensor access to blood in the cuvette.6. The cuvette of claim 5 , wherein the window retainer includes legs extending generally perpendicular to the surface and forming window retainer apertures that assist with coupling the window retainer to the cuvette body.7. The cuvette of claim 6 , wherein the cuvette body includes teeth configured to engage the window retainer apertures to couple the window retainer to the cuvette body.8. The cuvette of claim 7 , wherein the window ...

CELL CULTURE FLASKS, SENSOR INSERTS, AND SYSTEMS AND METHODS COMPRISING THE SAME

Embodiments disclosed herein relate to novel flasks, methods and systems employing said novel flasks, and sensors for use within said flasks and systems. In some embodiments, flasks and systems described herein are used to culture cells and/or detect changes in pH levels or dissolved oxygen and/or carbon dioxide levels in a cell culture sample resulting from the growth of living cells contained within a reaction vessel such as a flask. The devices may be used to detect changes in pH and dissolved oxygen levels in a liquid contained in the reaction vessel due to growth of living cells. 1. An inverted cell culture flask system for culturing cells comprising:a flask oriented in an inverted fashion,a vacuum pump,a stopper,a light scattering source,a detector system, andat least one optical sensor operably connected to the detector system.2. The system according to claim 1 , wherein the light scattering source scatters infrared or visible light.3. The system according to claim 1 , wherein the light scattering source scatters infrared light.4. The system according to claim 1 , wherein the stopper houses the light scattering source.5. The system according to claim 1 , wherein the stopper houses the at least one optical sensor operably connected to the detector system.6. The system according to claim 1 , further comprising a rotation unit or shaking unit configured to rotate or shake the flask.7. A method of culturing cells comprising culturing cells in a flask oriented in an inverted fashion claim 1 , wherein the flask comprises a stopper claim 1 , the stopper comprising a light scattering source and one or more optical sensors claim 1 , operably connected to a detector system.8. The method according to claim 7 , wherein the light scattering source scatters infrared or visible light.9. The method according to claim 7 , wherein the light scattering source scatters infrared light.10. The method according to claim 7 , further comprising evacuating air/gas from the flask via ...

POLYMERIC ORGANIC NANOPARTICLES WITH ENHANCED EMISSION

The present disclosure relates to luminescent including photon up-conversion nanoparticles. These nanoparticles dude a polymeric organic matrix, at least one light emitter distributed within this matrix, a stabilizing agent, and at least one metal particle enclosed within the matrix, wherein the metal particles are plasmonic nanoparticles. The present disclosure further relates to methods of manufacture and to uses of such nanoparticles. 1A nanoparticle (NP) , comprising:a polymeric organic matrix,at least one light emitter distributed within said matrix,a stabilizing agent, andat least one metal nanoparticle enclosed within said matrix, and/orat least one antioxidant distributed in the said matrix,wherein said at least one metal nanoparticle is a plasmonic nanoparticle.2. The nanoparticle according to claim 1 , wherein said polymeric organic matrix is composed of a material or a combination of materials selected from the group consisting of polyacrylonitriles claim 1 , polystyrenes and oligostyrenes claim 1 , styrene copolymers claim 1 , styrene-butadiene copolymers claim 1 , polystyrene-based elastomers claim 1 , polyethylenes and oligoethylenes claim 1 , polyphenylenes and polyphenylene dendrimers claim 1 , polypropylenes claim 1 , polytetrafluoroethylenes claim 1 , extended polytetrafluoroethylenes claim 1 , polyacrylates claim 1 , polymethylmethacrylates claim 1 , ethylene-co-vinyl acetates claim 1 , polysiloxanes claim 1 , their copolymers as well as substituted and modified polysiloxanes claim 1 , polyethers claim 1 , polyurethanes claim 1 , polyether-urethanes claim 1 , polyethylene terephthalates and polysulphones claim 1 , or any copolymers of the listed polymers.3. The nanoparticle according to claim 1 , wherein said light emitter is capable of emitting light by luminescence.4. The nanoparticle according to claim 1 , wherein said nanoparticle is capable of photon up-conversion.5. The nanoparticle according to claim 1 , wherein said nanoparticle further ...

NUCLEIC ACID SEQUENCE MEASUREMENT DEVICE, NUCLEIC ACID SEQUENCE MEASUREMENT METHOD, AND NUCLEIC ACID SEQUENCE MEASUREMENT APPARATUS

When the target () is not supplied, the binding via the binding part is maintained, and when the donor fluorescent molecule () is excited, energy is transferred to the acceptor fluorescent molecule () that is close to the donor fluorescent molecule (). Then, the acceptor fluorescent molecule () exhibits fluorescence. When the target () is supplied, the target () is bound to the detection part to release the binding via the binding part, and the acceptor fluorescent molecule () is separated from the donor fluorescent molecule (). Then, the donor fluorescent molecule () exhibits fluorescence.

Proteoform Specific Process Validation

A system and method is provided for validating the manufacturing process for the production of complex biological compositions, and particularly for providing process validation information for evaluation by a federal regulatory agency. The system and method continuously and chronologically assess the concentration of proteoforms within the biological composition as it is being produced in a fermentor. Samples from the fermentor are analyzed in a pre-selected array of analysis columns, with data generated by the columns being accumulated and evaluated, and particularly compared with data from previous stages in the production process. A continuous process validation system includes top-down and bottom-up analysis sectors, each including a plurality of different analysis columns that can be selected by the controller for a particular biological composition and a particular production process.

Measurement of bilirubin concentration in blood samples

A method for measuring bilirubin concentration in a sample includes preparing a sensing element, where the sensing element may include a plurality of carbon dots, adding the sample to the sensing element, where the sample may include a plurality of bilirubin molecules, obtaining a first grayscale image of the sensing element under ultra-violate (UV) irradiation, irradiating visible light with a wavelength between 470 nm and 490 nm on the sensing element, obtaining a second grayscale image of the sensing element under ultra-violate (UV) irradiation, calculating a light intensity difference by calculating a difference between a first average light intensity of the first grayscale image and a second average light intensity of the second image, and determining the bilirubin concentration based on a correlation between the bilirubin concentration and the light intensity difference.

METHOD OF DETECTING MULTIPLE TARGETS BASED ON SINGLE DETECTION PROBE USING TAG SEQUENCE SNP

The present invention relates to a method of detecting multiple targets based on a single detection probe, and more particularly to a method of detecting multiple targets by amplifying each target with primers including an SNP-containing tag sequence, hybridizing the amplification products with a single detection probe capable of binding to the tag sequences and designed such that melting temperatures are different from each other, and analyzing melting curves. A method of detecting multiple targets according to the present invention enables the detection of multiple targets using a single probe, and thus is useful for detecting multiple targets because false positives are reduced and multiple targets are detectable with high sensitivity and at a rapid rate. 1. A method of detecting multiple targets , the method comprising the steps of:a) obtaining DNA from a sample containing multiple targets;b) amplifying multiple target nucleic acids using n primer sets capable of respectively amplifying n multiple target nucleic acids (wherein n is an integer of 2 to 20);c) hybridizing the n amplification products with a single detection probe capable of hybridizing with the n amplification products; andd) analyzing a melting curve of each of the n reaction products hybridized in process c) to determine the presence or absence of the target nucleic acids,wherein each of the n primer sets comprisesa forward primer and a reverse primer comprising a tag sequence,wherein the tag sequences are designed such that melting temperatures of the n hybridized reaction products are different from each other.2. The method according to claim 1 , wherein the melting temperature difference ranges from 2° C. to 40° C.3. The method according to claim 1 , wherein in step b) claim 1 , p primer sets capable of respectively detecting p targets (wherein p is an integer of 1 to 20) are further included claim 1 , and in step c) claim 1 , a detection probe capable of hybridizing with all of the p ...

METHOD FOR THE DETECTION OF ANALYTES VIA LUMINESCENCE QUENCHING

A sensing element for use in the detection of an analyte based on a luminescent response, the sensing element comprising a luminescent triaryl amine compound provided as a coating on a substrate. 1. A sensing element for use in the detection of an analyte based on a luminescent response , the sensing element comprising a luminescent triaryl amine compound provided as a coating on a substrate.2. A sensing element according to claim 1 , wherein the coating has a thickness of up to 100 nm.3. A sensing element according to claim 1 , wherein the coating is provided on the internal surface of a capillary tube.4. A sensing element according to claim 3 , wherein the capillary tube has an internal diameter of from 100 Iμm to 1 mm and a length of up to 100 mm.5. A sensing element according to claim 1 , wherein the triaryl amine compound comprises a conjugated molecular structure bound to each of three aryl groups of a triaryl amine moiety.7. A sensing element according to claim 6 , at least one of R claim 6 , R claim 6 , and Ris a dendron.9. A sensor device for the detection of an analyte based on luminescent response claim 1 , the sensor device comprising a sensing element as claimed in .10. A sensor device according to claim 9 , comprising an excitation source to supply electromagnetic radiation that interacts with the triaryl amine compound claim 9 , a light detector for receiving light from the triaryl amine compound claim 9 , a component that allows the output of the light detector to be visualized or otherwise represented for interpretation and one or more temperature control elements for regulating and detecting the temperature of parts of the device.11. A method of detecting an analyte claim 9 , which method comprises:(i) allowing a luminescent compound comprising a triaryl amine moiety to interact with the analyte and measuring the luminescent properties of the compound in the presence of the analyte;(ii) detecting a difference between the luminescent properties ...

OPTICAL SENSOR ELEMENT

The invention relates to an optical sensor element, comprising indicators (), selected from luminescence-active means that are of the same type or different, and indicator protectors (), and to a sensor, comprising at least one such sensor element, an energy source that excites the luminescence emission of the indicators, and a detector unit, wherein the sensor element or sensor is suitable for detecting molecular oxygen in a gaseous or liquid medium and/or for determining the molecular oxygen content of a gaseous or liquid medium and at least one layer of the sensor element bearing the indicator protectors is designed in such a way that the diffusion rate of the molecular oxygen formed on the indicator protectors by means of the reduction of strong oxidants back into the medium is greater than the diffusion rate of molecular oxygen from the medium in the direction of the at-least-one layer bearing the indicator molecules. 1. An optical sensor element comprising:at least one indicator composed of a luminescence-active agent; andat least one indicator protector.2. The optical sensor element according to claim 1 , characterized in that the element has at least two layers claim 1 , and the at least one indicator and the at least one indicator protector are arranged in at least one of the at-least-two layers of the element.3. The optical sensor element according to claim 2 , characterized in that the at least one indicator and the at least one indicator protector are arranged in different layers of the element claim 2 , and the element comprises at least one indicator-bearing layer and at least one indicator protector-bearing layer.4. Optical sensor element according to claim 3 , characterized in that the at-least-one indicator protector-bearing layer is substantially free of indicators.52. Optical sensor element according to claim 2 , characterized in that the at least one indicator protector is arranged in a layer of the element that faces towards the medium and the ...

NUCLEIC ACID BASED BIOSENSOR AND METHODS THEREOF

The present disclosure relates to oligonucleotide biosensors that bind to a fluorophore through a reporter domain and to one or more target ligand(s) through one or more target domain(s), which is connected to the reporter domain through one or more linker domain(s). The binding of the target ligand to the target domain affects the fluorescence of the fluorophore when excited by the appropriate wavelength of energy, either by causing dimming or allosteric fluorescence. Methods of selecting biosensors and their use to detect target ligands are also described. 1. An oligonucleotide biosensor for detecting a target ligand in a sample , comprising:a reporter domain having a fluorophore binding region and an open stem region;one or more linker domains attached between the reporter domain and a target domain; andone or more target domains attached to a linker domain or another target domain.2. The biosensor of claim 1 , wherein the oligonucleotides are selected from the group comprising of DNA claim 1 , RNA claim 1 , PNA claim 1 , LNA claim 1 , and UNA.3. The biosensor of claim 1 , wherein the reporter domain is a Mango core.4. The biosensor of claim 3 , wherein the Mango core is Mango I.5. The biosensor of claim 1 , wherein the reporter domain binds to a molecule defined by Formula I or II.6. The biosensor of claim 1 , wherein the reporter domain has a binding affinity of at least about 400 nM.7. The biosensor of claim 1 , wherein the reporter domain increases the brightness of a fluorophore by at least 7 claim 1 ,000 M/cm.8. The biosensor of claim 1 , wherein the reporter domain is represented by the RNA sequence:{'sub': 1', '1', '2', '2', '3, 'claim-text': represents no nucleotide (gap);', 'K represents U or G;', 'S represents C or G,', 'R represents A or G;', 'W represents A or U;', 'H represents A, C, or U;', 'N represents A, C, G, or U; and', '@ represents N or no nucleotide;', 'wherein/between the brackets ( ) represents an alternative;', {'sub': 1', '2', '3, ' ...

Optical Biosensor

Provided herein is an optical biosensor for detecting a target bioanalyte in a sample. The biosensor includes: a porous silicon or alumina substrate having a surface and a detection agent immobilised on the surface. The detection agent includes a sensing domain and a signaling domain, the sensing domain having a linker capable of interacting with the target bioanalyte and the signaling domain having a luminescence donor and a luminescence acceptor wherein the luminescence donor and the luminescence acceptor are connected by the linker and are optically coupled in the absence of the target bioanalyte. Emission of light from the luminescence donor is substantially quenched by the luminescence acceptor, and interaction of the target bioanalyte with the linker results in optical un-coupling of the luminescence donor and the luminescence acceptor to thereby result in light emission from the luminescence donor. 1. An optical biosensor for detecting a target bioanalyte in a sample , the biosensor comprising:a porous silicon or alumina substrate comprising a surface and a detection agent immobilised on the surface, the detection agent comprising a sensing domain and a signaling domain, the sensing domain comprising a linker capable of interacting with the target bioanalyte and the signaling domain comprising a luminescence donor and a luminescence acceptor wherein the luminescence donor and the luminescence acceptor are connected by the linker and are optically coupled in the absence of the target bioanalyte such that emission of light from the luminescence donor is substantially quenched by the luminescence acceptor, and interaction of the target bioanalyte with the linker results in optical un-coupling of the luminescence donor and the luminescence acceptor to thereby result in light emission from the luminescence donor; anda plurality of light interacting pores on the surface of the substrate, wherein the pores are configured to interact with the light emission from the ...

MEASURING DEVICE AND SYSTEM FOR PERFORMING MELTING CURVE ANALYSIS OF A DNA MICROARRAY AND UTILIZATION OF A FLUORESCENCE DETECTOR ARRAY FOR ANALYSIS

What is shown is a measuring device for simultaneously analyzing melting curves of DNA samples in DNA microarrays with the aid of a fluorescence detector array. Embodiments show monitoring of melting curves of DNA microarrays (dynamic fluorescence measurement) by means of silicon photomultipliers (SiPM) or other photodetectors such as, e.g., PIN diodes (positive intrinsic negative diodes), avalanche photodiodes (APD), or photomultiplier tubes (PMT). The DNA microarray is applied, along with a thin-film heating element, to a substrate made of plastic or glass or is integrated in a microfluidic channel. 1. A measuring device for performing melting curve analysis of at least one DNA sample , the measuring device comprising:a two-dimensional DNA microarray for accommodating the at least one DNA sample;a two-dimensional fluorescence detector array comprising at least one fluorescence detector; andan integrated heating element for heating the DNA sample applied to the DNA microarray.2. The measuring device as claimed in claim 1 , wherein the fluorescence detector comprises a silicon photomultiplier.3. The measuring device as claimed in claim 1 , wherein the fluorescence detector comprises an avalanche photodiode.4. The measuring device as claimed in claim 1 , wherein the fluorescence detector comprises a PIN diode.5. The measuring device as claimed in claim 1 , wherein the fluorescence detector comprises a photomultiplier tube and an optical waveguide.6. The measuring device as claimed in claim 1 , wherein the fluorescence detector is configured to detect a change in the fluorescence intensity of the at least one DNA sample that is caused by a change in a nucleic-acid structure that is due to a temperature rise.7. The measuring device as claimed in claim 1 , wherein the fluorescence detector array comprises a plurality of fluorescence detectors or a monolithic multichannel fluorescence detector.8. The measuring device as claimed in claim 7 , wherein each of the plurality ...

pH-MODULATED QUENCHERS OF FLUORESCENCE

The present disclosure relates to a compound of formula (I) 2. The compound or salt of claim 1 , wherein each{'sub': '2', 'sup': 1', '2', '3', '4, 'X is C—H, A is COR, and B is B, B, or B;'}{'sup': 2', '3', '4, 'each X is C—H, A is CN, and B is B, B, or B;'}{'sup': 6', '2', '3', '4, 'each X is C—H, A is COR, and B is B, B, or B;'}{'sup': 1', '1', '6, 'sub': '2', 'at least one X is N or C—Zand A is COR, CN or COR; or'}{'sub': 2', '2, 'sup': 2', '3', '4', '5', '5', '7, 'A is COR, C(O)NRR, S(O)R, SOR, C(O)R, or CO(NHS).'}3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)11. (canceled)12. (canceled)13. (canceled)14. (canceled)15. (canceled)16. (canceled)17. (canceled)18. (canceled)19. (canceled)20. (canceled)21. (canceled)23. (canceled)24. (canceled)25. (canceled)26. (canceled)27. (canceled)28. (canceled)29. The compound or salt of claim 1 , wherein each X is C—H.30. The compound or salt of claim 1 , wherein each Y is C—H.31. The compound or salt of claim 1 , wherein at least one occurrence of X is N.32. The compound or salt of claim 1 , wherein at least one occurrence of Y is N.33. (canceled)34. (canceled)35. (canceled)36. (canceled)41. The covalent conjugate of claim 40 , wherein each X is C—H.42. The covalent conjugate of claim 40 , wherein each Y is C—H.43. The covalent conjugate of claim 40 , wherein at least one occurrence of X is N.44. The covalent conjugate of claim 40 , wherein at least one occurrence of Y is N.45. (canceled)46. (canceled)47. (canceled)48. (canceled)49. The covalent conjugate or salt of any one of claim 40 , wherein the Conjugated Species is a protein claim 40 , an antibody claim 40 , a peptide claim 40 , a nucleic acid claim 40 , an oligonucleotide claim 40 , a solid support claim 40 , a soluble polymer claim 40 , an insoluble polymer claim 40 , a dendrimer claim 40 , a saccharide claim 40 , a fatty acid claim 40 , a lipid claim 40 , or a phospholipid.50. (canceled)51. (canceled)52. (canceled)53. ( ...

EX VIVO PROTEASE ACTIVITY DETECTION FOR DISEASE DETECTION/DIAGNOSTIC, STAGING, MONITORING AND TREATMENT

The present application provides compositions and methods for determining a disease or condition in a subject. The method comprises contacting a body fluid with a molecule comprising a reporter thereof and the reported is cleaved by an agent in the body fluid. Diseases and conditions that can be determined by the method are also described. 1. A method comprising: wherein said molecule comprises a reporter, and', 'wherein said molecule reacts with an agent from said body fluid, causing said reporter to form a detectable signal,, 'contacting a body fluid sample from a subject with a molecule ex vivo,'}detecting a rate of formation or an amount of said detectable signal,determining a disease or condition of said subject based on said detection,wherein said disease or condition is a certain fibrosis stage or a certain nonalcoholic fatty liver disease activity score (NAS) of Non-alcoholic steatohepatitis (NASH).2. The method of claim 1 , wherein said body fluid sample is selected from the group consisting of blood claim 1 , plasma claim 1 , bone marrow fluid claim 1 , lymphatic fluid claim 1 , bile claim 1 , amniotic fluid claim 1 , mucosal fluid claim 1 , saliva claim 1 , urine claim 1 , cerebrospinal fluid claim 1 , spinal fluid claim 1 , synovial fluid claim 1 , semen claim 1 , ductal aspirate claim 1 , feces claim 1 , stool claim 1 , vaginal effluent claim 1 , lachrymal fluid claim 1 , tissue lysate and patient-derived cell line supernatant.3. The method of claim 1 , wherein said body fluid sample comprises a rinse fluid claim 1 , a conditioning media or buffer claim 1 , a swab viral transport media claim 1 , a saline claim 1 , a culture media claim 1 , or a cell culture supernatant.4. The method of claim 3 , wherein said rinse fluid is selected from the group consisting of a mouthwash rinse claim 3 , a bronchioalveolar rinse claim 3 , a lavage fluid claim 3 , a hair wash rinse claim 3 , a nasal spray effluent claim 3 , a swab of any bodily surface claim 3 , orifice ...

WAVELENGTH SCANNING APPARATUS AND METHOD OF USE THEREOF

A wavelength scanning apparatus that detects at least four different fluorescent emission wavelengths simultaneously or nearly simultaneously is described. The wavelength scanning apparatus includes a heating block having at least four sample wells, each sample well configured for receiving a sample, at least four excitation activation apertures, and at least four fluorescence emission discharge apertures. The wavelength scanning apparatus also includes an analysis scanner having at least four light sources, where the at least four light sources excite at least four fluorophores, at least four excitation light filters that filter out light except that of the desired excitation wavelength/s, at least four fluorescence emission light filters that filter out light except that of the desired fluorescent emission wavelengths, and at least four photodetectors to detect light of the desired fluorescent emission wavelengths. 1. A wavelength scanning apparatus for measuring fluorescence emission of a plurality of nucleic acid samples , comprising: 'the reset button is in mechanical communication with the reset trigger;', 'a reset button and a reset trigger, wherein'}, 'a structural support, the structural support comprising'} [ 'each of the plurality of sample wells forms an opening on a top side of the heating block,', 'a plurality of sample wells, wherein'}, 'a plurality of excitation activation apertures, and', 'a plurality of fluorescence discharge apertures, wherein', 'each of the plurality of sample wells transitions to a corresponding excitation activation aperture of the plurality of excitation activation apertures and a corresponding fluorescence discharge aperture of the plurality of excitation activation apertures, and wherein', 'each of the plurality of excitation activation apertures forms an opening on a bottom side of the heating block and each of the plurality of fluorescence discharge apertures forms an opening on a front side of the heating block;, 'a ...

FRESHNESS LABEL WITH AGGREGATION-INDUCED PHOSPHOR

A freshness label in an embodiment includes a base material having a fluid retention capacity and a phosphor layer on a surface of the base material. The phosphor layer includes an aggregation-induced phosphor. The base material has a porous or mesh structure. The aggregation-induced phosphor comprises a phosphor having a polar functional group or the like. The freshness label can be used to detect spoilage and/or deterioration of fresh food according to changed in fluorescence intensity provided by the aggregation-induced phosphor. 1. A freshness label , comprising:a base material that retains fluids; anda phosphor layer on a surface of the base material, the phosphor layer including an aggregation-induced phosphor.2. The freshness label according to claim 1 , wherein the phosphor layer is quenchable with water.3. The freshness label according to claim 1 , wherein the base material has a porous structure.4. The freshness label according to claim 1 , wherein the base material has a mesh structure.5. The freshness label according to claim 1 , wherein the aggregation-induced phosphor comprises a phosphor having a polar functional group.6. The freshness label according to claim 5 , wherein the polar functional group includes an acidic functional group.7. The freshness label according to claim 5 , wherein the polar functional group includes a basic functional group.9. The freshness label according to claim 1 , wherein water is retained in the base material.10. The freshness label according to claim 1 , wherein an acidic aqueous solution is retained in the base material.11. A method for identifying a freshness level of a food product with a freshness label including a base material that retains fluids and a phosphor layer on a surface of the base claim 1 , the phosphor layer including an aggregation-induced phosphor claim 1 , the method comprising:immersing the freshness label in an aqueous solution or water; andafter said immersing, packing the food product along with ...

DETECTION OF ACRYLIC ACID

There is provided a method for detecting the presence or absence of acrylic acid or its derivatives thereof in a sample, the method comprising the steps of: (a) introducing a probe comprising a diaryltetrazole compound to the sample; (b) exposing said sample to light; and (c) detecting the presence or absence of acrylic acid or its derivatives thereof in the sample based on fluorescence emitted by the sample after step (c). 1. A method for detecting the presence or absence of acrylic acid or its derivatives thereof in a sample , the method comprising:(a) introducing a probe comprising a diaryltetrazole compound to the sample;(b) exposing said sample to light; and(c) detecting the presence or absence of acrylic acid or its derivatives thereof in the sample based on fluorescence emitted by the sample after said operation (c).4. (canceled)5. The method according to claim 1 , wherein said probe is biotinylated.6. The method according to claim 1 , wherein said exposure occurs at a wavelength in the range of 10 nm to 1 mm.7. The method according to claim 6 , wherein said wavelength is 302 nm.8. The method according to claim 1 , wherein the operation of exposure further comprises the operation of forming a reactive intermediate.9. The method according to claim 8 , wherein said reactive intermediate is a compound comprising a nitrile imine dipole.10. The method according to claim 1 , wherein said fluorescent sample after exposure to light is a cycloadduct comprising a fluorescent pyrazoline.11. The method according to claim 1 , wherein said acrylic acid or its derivatives thereof is present at a concentration of at least 100 nM before introducing the probe to said sample.12. The method according to claim 1 , wherein the diaryltetrazole compound introduced is present at a concentration of at least 1 nM in said sample.13. The method according to claim 1 , wherein the exposure of said sample to light occurs under alkaline conditions.14. The method according to claim 1 , ...

SELECTIVE DELIVERY MOLECULES AND METHODS OF USE

Disclosed herein is a selective delivery molecule comprising: (a) an acidic sequence (portion A) which is effective to inhibit or prevent the uptake into cells or tissue retention, (b) a molecular transport or retention sequence (portion B), and (c) a linker between portion A and portion B, and (d) at least one cargo moiety. 1. A selective delivery molecule of Formula I , having the structure:{'br': None, 'sub': A', 'A', 'M', 'B', 'B, 'i': c', 'c', 'c, '[D-]-A-[-M]-X-B-[-D]\u2003\u2003 Formula I'} X is a cleavable linker;', 'A is a peptide with a sequence comprising 5 to 9 acidic amino acids;', 'B is a peptide with a sequence comprising 7 to 9 basic amino acids;', {'sub': A', 'B', 'M, 'c, c, and care each independently 0-1 amino acid;'}, 'M is a polyethylene glycol (PEG) polymer; and', {'sub': A', 'B, 'Dand Dare each independently an imaging agent; and'}], 'wherein,'}{'sub': M', 'A', 'A', 'B', 'B, 'wherein [c-M] is bound to at any position on A or X, [D-c] is bound to any amino acid on A, and [c-D] is bound to any amino acid on B.'}2. The molecule of claim 1 , wherein A and B do not have an equal number of acidic and basic amino acids.3. The molecule of claim 2 , wherein the number of basic amino acids in B is greater than the number of acidic amino acids in A.4. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates.5. The molecule of claim 1 , wherein B is a peptide comprising 8 or 9 consecutive arginines.6. The molecule of claim 1 , wherein A is a peptide comprising 5 or 9 consecutive glutamates and B is a peptide comprising 8 or 9 consecutive arginines.7. The molecule of claim 6 , wherein A is a peptide comprising 5 consecutive glutamates and B is a peptide comprising 8 consecutive arginines.8. The molecule of claim 1 , wherein c claim 1 , c claim 1 , and care each independently selected from a naturally-occurring amino acid or a non-naturally-occurring amino acid.9. The molecule of claim 8 , wherein c claim 8 , c claim 8 , and ...

NUCLEOSOME SUBSTRATE ASSAYS

The present invention relates to methods for determining a binding and/or functional interaction of a protein of interest with a nucleosomal substrate wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern. Further, the invention relates to nucleosomal substrates, wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern, and to methods for providing such nucleosomal substrates. 1. A method for determining a binding and/or functional interaction of a protein of interest with a nucleosomal substrate comprising DNA wrapped around histone octamers , wherein the method comprises:(a) forming a composition of matter comprising the protein of interest and the nucleosomal substrate; and(b) determining a value indicative for the binding and/or functional interaction of the protein of interest with the nucleosomal substrate by FRET detection;wherein at least one histone type of the nucleosomal substrate has a homogenous post-translational modification pattern.2. The method of claim 1 , wherein the FRET detection is TR-FRET detection.3. The method of claim 1 , wherein the composition of matter further comprises a molecule which is a candidate for modulating the binding and/or functional interaction of the protein of interest with the nucleosomal substrate.4. The method of claim 1 , wherein the DNA wrapped around histone octamers is arranged in oligonucleosomes.5. The method of claim 1 , wherein the nucleosomal substrate is labeled with a FRET donor and/or FRET acceptor.6. The method of claim 5 , wherein the DNA of the nucleosomal substrate is labeled with a FRET donor and/or FRET acceptor.7. The method of claim 5 , wherein the histones of the nucleosomal substrate are labeled with a FRET donor and/or FRET acceptor.8. The method of claim 1 , wherein the protein of interest is labeled with a FRET acceptor and the nucleosomal substrate is ...

DIGITAL AMPLIFICATION ASSAYS WITH UNCONVENTIONAL AND/OR INVERSE CHANGES IN PHOTOLUMINESCENCE

Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes. 1. A method of analysis , the method comprising:forming isolated volumes each including a portion of the same sample, wherein each volume also includes (i) a probe having a label, (ii) a sink having a quencher and configured to hybridize with the probe to quench the label, and (iii) a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe, and wherein only a subset of the volumes contain at least one copy of a target from the sample;performing an amplification reaction in the volumes to generate an amplicon corresponding to the target, wherein the separator hybridizes with the amplicon, and wherein the separator is extended or degraded in target-positive volumes by the amplification reaction; anddetecting photoluminescence of the label from the volumes, wherein the photoluminescence of target-positive volumes is less than that of target-negative volumes.2. The method of claim 1 , wherein the separator hybridizes with the sink to block hybridization of the sink with the probe.3. The method of ...

NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE

Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems. 176.-. (canceled)77. A method of measuring enzymatic activity , the method comprising:combining a luminogenic protein, a deprotecting enzyme-of-interest, and a protected luminophore into a composition; anddetecting light produced from the composition, wherein the luminogenic protein is a CBR variant encoded by a polynucleotide having at least 80% amino acid sequence identity to SEQ ID NO: 1 and comprises at least one amino acid substitution at a position corresponding to position 4, 16, 34, 47, 51, 52, 55, 72, 73, 74, 79, 82, 83, 87, 89, 104, 109, 113, 117, 119, 124, 130, 131, 133, 136, 144, 146, 156, 159, 170, 179, 186, 200, 211, 218, 224, 225, 226, 228, 229, 234, 247, 251, 252, 253, 255, 280, 281, 285, 308, 309, 310, 319, 329, 334, 335, 337, 346, 348, 349, 350, 352, 354, 355, 358, 363, 370, 377, 390, 393, 394, 400, 401, 409, 412, 420, 422, 431, 437, 439, 444, 445, 453, 455, 467, 471, 473, 479, 484, 489, 496, 501, 503, 508, 516, 528, 531, 535, 537, 539, or combination thereof, of SEQ ID NO: 1, and wherein the variant CBR polypeptide has at least one of enhanced luminescence, altered light emission wavelength, altered substrate specificity, or a combination thereof, as compared to a CBR polypeptide of SEQ ID NO: 1.79. The method of claim 77 , wherein the enzymatic activity of the deprotecting enzyme-of-interest is measured in a live claim 77 , intact non-human animal.80. The method of claim 77 , wherein the deprotecting enzyme-of-interest is a protease enzyme claim 77 , a cytochrome P450 enzyme claim 77 , a monoamine oxidase ...

Measurement of a dynamic system

A method for performing measurements includes providing relative movement between a receptacle and a cover over a measurement interval comprising multiple non-overlapping time periods. A distance between the receptacle and the cover changes along a first axis over at least a portion of each time period. An apparatus for performing measurements includes a receptacle comprising a substrate and a plurality of wells within the substrate, and a cover. The cover includes: (1) a device configured to engage with the receptacle, (2) a control subsystem configured to provide relative movement between the receptacle and the cover, and (3) a measurement subsystem.

ANALYSIS OF VIABLE AND NONVIABLE CELLS

Provided are methods, compositions, and kits for selectively analyzing a sample for viable cells and nonviable cells. For example, the method may include dyeing viable cells and/or nonviable with a membrane-permeable fluorescent dye, and dyeing nonviable cells with a membrane-impermeable fluorescent quenching dye. The method may include illuminating to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The method may include quenching at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The method may include selectively analyzing the sample for the viable and nonviable cells. 1. A method for selectively analyzing a sample for viable cells and nonviable cells , comprising:providing a sample comprising one or more viable cells and/or one or more nonviable cells;contacting the sample with a membrane-permeable fluorescent dye and a membrane-impermeable fluorescent quenching dye, the contacting effective to cause: the one or more viable cells and/or the one or more nonviable cells to be dyed by the membrane-permeable fluorescent dye, and the one or more nonviable cells to be dyed with the membrane-impermeable fluorescent quenching dye, the membrane-permeable fluorescent dye characterized by a first fluorescent excitation band and a first fluorescence emission band and the membrane-impermeable fluorescent quenching dye characterized by a second fluorescent excitation band and a second fluorescence emission band; andilluminating the sample under conditions effective to cause: emitting of the first fluorescence emission band from the one or more viable cells dyed by the membrane-permeable fluorescent dye, emitting of the second fluorescence emission band from the one or more nonviable cells dyed by the membrane-impermeable fluorescent quenching dye, and ...

METHOD AND DEVICE FOR DETERMINING CONCENTRATION, CROSSTALK AND DISPLACEMENT FLUORESCENCE CROSS CORRELATION SPECTROSCOPY

The present invention provides a FCCS method for determining the concentration and/or the diffusion coefficient of at least a first labeled species, a second labeled species and/or a complex between said first and second labeled species, in a system, wherein the method comprises the steps of determining a cross-talk parameter K, wherein K is the ratio between the brightness of the first labeled species and the second labeled species at the centre of each focus, as detected for both species in the channel for detecting the second labeled species; using the cross talk parameter K for determining a displacement parameter rand using K, r, or both K and rfor determining the concentration and/or the diffusion coefficient of said first and/or a second labeled species and/or a complex between said first and second labeled species. 145.-. (canceled)46. A FCCS method for determining the concentration and/or the diffusion coefficient of at least a first labeled species , a second labeled species and/or a complex between said first and second labeled species , in a system , with a FCCS apparatus comprising a first laser for exciting the first labeled species , the same or a second laser for exciting the second labeled species as well as a first channel for detecting fluorescence from the first labeled species and a second channel for detecting fluorescence from the second labeled species , wherein the method comprises the steps ofa) determining a cross-talk parameter K, wherein K is the ratio between the brightness of the first labeled species and the second labeled species at the centre of each focus, as detected for both species in the channel for detecting the second labeled species;{'sub': o', '0, 'b) optionally determining a displacement parameter r, wherein ris the displacement between the two lasers of the FCCS apparatus in the lateral dimension if a second laser is used for exciting the second labeled species, and'}{'sub': o', 'o, 'c) using K, ror both K and rfor ...

ULTRAFAST SEQUENCING OF BIOLOGICAL POLYMERS USING A LABELED NANOPORE

Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer. 116-. (canceled)17. A method for sequencing nucleic acid polymers comprising:translocating nucleic acid polymers each in single file through a plurality of nanopores, wherein the nucleic acid polymers each comprise nucleotide monomers labeled with acceptor labels and the nanopores each have attached one or more donor labels each capable of fluorescence resonanant energy transfer (FRET) after illumination with radiation and wherein acceptor labels come in close proximity with donor labels as nucleotide monomers exit the nanopores so that a FRET energy exchange interaction can occur between donor labels and acceptor labels;exciting donor labels by illumination so that energy from excited donor labels is transferred by FRET to the acceptor labels of nucleotide monomers exiting the nanopores, wherein such energy transfer causes the acceptor labels to emit radiation at a lower energy than the radiation that was used to excite the donor labels;detecting the radiation emitted by the acceptor labels as a result of the energy transfer, wherein the radiation emitted by the acceptor labels identify the nucleotide monomer to which it is attached; anddetermining nucleotide sequences of the nucleic acid polymers based on the detection of the emitted radiation ...

Thermally Resolved Molecule Assays

Provided herein are compositions and methods including the step of thermally scanning a sample that can be used and implemented to detect the presence of and/or concentration of a molecule in a sample. 1. A method of target molecule detection comprising:contacting a sample with a first probe, wherein the first probe is configured to bind a target molecule;contacting the sample with a second probe, wherein the second probe is configured to bind the target molecule;forming a signal complex;thermally scanning the signal complex to form a temperature melting curve, the temperature melting curve comprising;a first melting temperature peak, where the first melting temperature peak corresponds to the melting temperature of the signal complex; anddetecting the target molecule via measuring a characteristic of the first melting temperature peak.2. The method of claim 1 , further comprising quantifying the amount of signal complex by the peak area of the first melting temperature peak.3. The method of claim 1 , further comprising forming a background complex and wherein the temperature melting curve further comprises a second melting temperature peak claim 1 , where the second temperature melting peak corresponds to the melting temperature of the background complex.4. The method of claim 1 , wherein the first probe is selected from the group consisting of:aptamers, antibodies or fragments thereof, proteins, and oligonucleotides.5. The method of claim 1 , wherein the second probe is selected from the group consisting of: aptamers claim 1 , antibodies or fragments thereof claim 1 , proteins claim 1 , and oligonucleotides.6. The method of claim 5 , wherein the first probe and the second probe are each directly bound to the target molecule in the signal complex.7. The method of claim 6 , wherein the first probe and the second probe are each selected from the group consisting of: aptamers claim 6 , antibodies or fragments thereof claim 6 , proteins claim 6 , and oligonucleotides.8 ...

SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES

A receptacle comprises opposed members, a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers, and a rigid frame supporting the opposed members at their peripheral edges. The frame comprises a front frame portion and a rear frame portion, and the peripheral edges of the opposed members are retained between the front and rear frame portions. An inlet port extends between the front and rear frame portions and is in fluid communication with one of the chambers. 1. A receptacle comprising:opposed members;a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers;a rigid frame supporting the opposed members at their peripheral edges, wherein the frame comprises a front frame portion and a rear frame portion, and wherein the peripheral edges of the opposed members are retained between the front and rear frame portions; andan inlet port that extends between the front and rear frame portions and is in fluid communication with one of the chambers.2. The receptacle of claim 1 , further including a port cover configured to close off the inlet port.3. The receptacle of claim 1 , wherein the frame comprises an identifying label.4. The receptacle of claim 1 , wherein the identifying label comprises a bar code.5. The receptacle of claim 1 , wherein the frame comprises a tab projecting from the frame to provide an appendage for grasping the receptacle.6. The receptacle of claim 5 , wherein the inlet port extends through the tab.7. The receptacle of claim 1 , wherein the frame comprises a front frame component and a rear frame component.8. The receptacle of claim 7 , wherein the front frame component and the rear frame component are injection molded and are connected to one another by ultrasonic welding.9. The receptacle of claim 7 , wherein the front frame component and the rear frame component are rectangular claim 7 ...

SYSTEM AND METHOD OF USING MULTI-CHAMBERED RECEPTACLES

A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material. The method includes the steps of causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber, applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; and preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber 1. A method of processing a sample in a receptacle comprising a plurality of chambers , wherein each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material , the method comprising:A. causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber;B. applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; andC. preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber during step B.2. The method of claim 1 , wherein the gas bubbles are prevented from flowing through the portal into the second chamber during step B by a wall extending from a peripheral boundary of the first chamber into an interior space of the first chamber.3. The method of claim 1 , wherein causing the gas bubbles to accumulate in a portion of the first chamber comprises orienting the receptacle so that the gas bubbles accumulate in an upper portion of the first chamber.4. The method of claim 1 , wherein the receptacle ...

Optochemical sensor unit and a method for the qualitative and/or quantitative determination of an analyte in a measuring medium with the sensor unit

An optochemical sensor unit including: an optical waveguide; a transmitting unit for emitting a first transmission signal for exciting a luminophore; a receiving unit for receiving a received signal comprising a signal component emitted by the excited luminophore; a measuring chamber for receiving a fluid, wherein the fluid includes magnetic microspheres; a membrane arranged between the measuring chamber and a measuring medium for exchanging an analyte between the measuring medium and the fluid in the measuring chamber, wherein the measuring diaphragm is impermeable to the magnetic microspheres; and an electromagnet for attracting magnetic microspheres to a sensor membrane with a fluid-contacting surface and/or to a fluid-contacting surface of the optical waveguide, or to a surface of a transparent substrate layer of the optical sensor unit that is connected to the optical waveguide.

METHOD FOR MEASURING OXYGEN AND APPARATUS FOR MEASURING OXYGEN

A measuring method for measuring dissolved oxygen includes performing a first measurement sequence, including: emitting a first stimulation signal onto a sensor for a first period; detecting a first detection signal; determining a phase shift between the first stimulation signal and the first detection signal; and calculating a first measured value based on the determined phase shift. Performing a second measurement sequence, including a second stimulation signal onto the sensor for a second period, wherein the second stimulation signal is different than the first stimulation signal; detecting a second detection signal; determining a decay time of the second detection signal; calculating a second measured value based on the decay time. The method further includes comparing the first measured value to the second measured value and correcting the first measured value when a difference between the first measured value and the second measured value is greater than a first limit value. 1. A method for measuring dissolved oxygen in a medium , the method comprising:providing an apparatus configured to measure dissolved oxygen, the apparatus comprising a computing unit connected to an optical sensor, the optical sensor including a light source, a sensitive layer and a detector; emitting a first stimulation signal from the light source onto the sensitive layer for a first time period;', 'detecting a first detection signal emitted by the sensitive layer using the detector;', 'determining a phase shift between the first stimulation signal and the first detection signal; and', 'calculating a first measured value based on the determined phase shift;, 'performing a first measurement sequence at least once, wherein the first measurement sequence comprises emitting a second stimulation signal from the light source onto the sensitive layer for a second time period, wherein the second stimulation signal is different than the first stimulation signal;', 'detecting a second detection ...

RAPID AND HIGH-SENSITIVE BACTERIA DETECTION

An improved method and related apparatus for detecting bacteria viability and drug effects using metabolic monitoring. A fluorescent material which is quenched by oxygen is co-localized with the target bacteria, and fluorescence signal is detected at the co-localized places. In some embodiments, the fluorescent material is a fluorescent nanoparticle mixed with the target bacteria in the sample, and co-localization is enhanced using centrifugation, electrophoresis, microflow path modified with antibodies, magnetic force, etc. In some other embodiments, the fluorescent material is a fluorescent film or 3-D matrix immobilized in the bacterial culture chamber, and bacteria in the sample is gathered into localized regions of the bacteria culture chamber where the fluorescent film or 3-D matrix is present by ways of centrifugation, electrophoresis or microflow path. Plasmonic nanoparticles with a metal core and plasmonic film with a metal film may be used as the fluorescent nanoparticles and fluorescent film. 1. A method for detecting live bacteria , comprising:providing a fluorescent material which is quenched by oxygen;co-localizing the fluorescent material with the bacteria in a region of a bacteria culture chamber;allowing the bacteria to grow; anddetecting a fluorescence signal emitted by the fluorescent material in the co-localized region.2. The method of claim 1 , wherein the fluorescent material has an affinity to the bacteria.3. The method of claim 1 , wherein the fluorescent material comprises fluorescent nanoparticles.4. The method of claim 3 , wherein each fluorescent nanoparticle comprises:a metal core;a spacer layer outside the metal core; anda fluorescent material outside the spacer layer.5. The method of claim 4 , wherein the metal core is made of gold claim 4 , silver claim 4 , or aluminum.6. The method of claim 3 , wherein the co-localizing step includes one or more of:(1) applying centrifugation to a sample containing the bacteria and the fluorescent ...

MEASUREMENT DEVICE THROUGH WHICH BREATHING GAS CAN FLOW FOR MEASURING GAS COMPONENTS OF THE BREATHING GAS

The invention relates to a measurement device for determining at least one gas component of a gas present in a measuring chamber of the measuring device, the measuring device comprising a housing enclosing the measuring chamber, at least one housing wall section of which housing being designed as an observation section for detecting electromagnetic radiation emanating from the observation section in a direction away from the measuring chamber, the observation section comprising at least one film layer, and the housing being designed as a plastic injection-moulded housing. The invention is characterised in that the observation section has at least one observation wall component comprising an injection-moulded observation body injection-moulded onto the at least one film layer, and the housing comprises the at least one observation wall component and an injection-moulded frame body injection-moulded onto the observation wall component. 1. A measuring device for measuring at least one gas constituent of a gas present in a measuring chamber of the measuring device , where the measuring device comprises:a housing surrounding the measuring chamber, of which at least one housing wall section is configured as an observation section for the acquisition of electromagnetic radiation emitted from the observation section in a direction away from the measuring chamber, where the observation section comprises at least one foil layer and where the housing is configured as a synthetic material injection molded housing,wherein the observation section exhibits at least one observation wall component, comprising an observation injection-molded body injected onto the at least one foil layer, where the housing comprises the at least one observation wall component and a frame injection-molded body injected onto the observation wall component.2. The measuring device according to claim 1 ,wherein the measuring chamber allows the flowthrough of gas along a virtual flow path.3. The measuring ...