09-07-2015 дата публикации
Номер: US20150192589A1
Принадлежит:
The invention provides method for imaging the activity of enzymes or bioactive molecules in cells using fluorescent probes with aggregation-induced emission properties and excited-state intramolecular proton transfer properties. The fluorescent probes prepared according to the invention are those of formula (I), 1. A method for imaging the activity of enzymes or bioactive molecules in cells using fluorescent probes with aggregation-induced emission properties and excited-state intramolecular proton transfer properties.2. The method according to wherein the fluorescent probe precursor has an organelle specific target group T and a reactive site R for reaction with enzymes or biomolecules.4. The method according to claim 3 , wherein in formula (I) claim 3 ,{'sub': 1', '6', '1', '6, 'X represents hydrogen, C-C-alkyl, C-C-alkynyl or heteroaryl having 5 to 6 ring members and 1 to 3 hetero atoms selected from O, N or S,'}{'sub': 1', '6', '1', '6', '1', '6', '1', '6', '1', '6', '5', '7', '1', '6', '1', '6', '1', '6, 'Y represents a direct bond between the two nitrogen atoms or represents an aryl having 6 or 10 carbon atoms in the aryl moiety or heteroaryl group having 5 to 6 ring members and 1 to 3 hetero atoms selected from O, N or S which are optionally substituted by one or more substituents selected from halogen, cyano, C-C-alkyl, C-C-perfluoroalkyl, C-C-alkoxy, aryl-oxy having 6 or 10 carbon atoms in the aryl moiety, C-C-alkenyl, C-C-alkynyl, C-C-cycloalkyl, C-C-alkylthio, amido, amino, C-C-monoalkylamino, di-C-C-alkylamino, carboxamide, hydroxy, mercapto, aryl having 6 or 10 carbon atoms in the aryl moiety or heteroaryl having 5 to 6 ring members and 1 to 3 hetero atoms selected from O, N or S,'}R represents an ethyl, acetyl, sulfate, 2-ethyl-5-nitrothiophene, arylboronate, p-aminophenol, homoallyl ether, diphenylphosphinyl, 2,4-dinitrobenzenesulfonyl, 1,4-diketone, carbohydrate, amino acid, glycosyl or peptide group,{'sub': 1', '6', '1', '6', '1', '6', '1', '6', '1 ...
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