Methods and devices for the long-term culture of hematopoietic progenitor cells
Номер патента: EP1015558A1
Опубликовано: 05-07-2000
Автор(ы): Mark J. Pykett, Michael Rosenzweig, Richard B. Kaplan
Принадлежит: Cytomatrix LLC
Опубликовано: 05-07-2000
Автор(ы): Mark J. Pykett, Michael Rosenzweig, Richard B. Kaplan
Принадлежит: Cytomatrix LLC
Реферат: The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.
Method for the ex vivo expansion of hematopoietic stem and progenitor cells
Номер патента: WO2013040644A1. Автор: Melinda L. TURSKY,Mark A. KIRKLAND. Владелец: CYTOMATRIX PTY LTD. Дата публикации: 2013-03-28.