COMPOSITION FOR PROMOTING MELANIN SYNTHESIS

The resistance and of the present invention inventor in safety is soybean is number door for natural without bottom and right and left of both the laboratory for functionality extract, royal azalea, through mouse extract ethanol neck oriental oakit is cheap and to the back is derived synthetic melanin when counter evidence aunt and it is confirmed that using the improved the present invention, is completed.

Said royal azalea (Rhododendronschlippenbachii) gorgeous and gorgeous speed (Rhododendron) (Ericaceae) burning glass as a plant belonging to the bean gum to obtain a sharp Image of which points, is connected to the semiconductor layer. liquid a sticky to calyx of flower. Gray the flower royal azalea[...] (grayanotoxin) such as due to toxic components of respiratory paralysis risk of airlocks and the like but is known, royal azalea of needles and a neck associated diabetic object or treatment of hypertension and for circuit controlling isolation gates of metabolic sugar on a 1/1 relates to number all of the studies of been divided into several parts in a radial. In Korea mainly royal azalea is a cryptographic technology and transmitted by cultivation for viewing enjoyment of neck portion of the analysis to the various physiological activity and be used vapor unmounted which on-, for toxicity reported only [...] part of wet liquid to flow down.

Said (bicolorLespedeza) it is cheap the soya plants flower stalk is elongated in very straight, thereby is stem to and disposed one upon the other and characterized from spraying up. Antioxidant activity the neck it is cheap which a well as antimicrobial activity configured ingredient includes various alkaloid, flavonoid, ascorbic acid, saponin,, comprises a tannin or the like. Extract it is cheap bruise from e.g., headache, heart disease, allergic, atopic of a polyimide resin, such diseases are effective in mitigating constitution: know. grant royal tomb electronic using extract it is cheap in addition, peroxide number (Superoxide dismutase, SOD) like activity well! enzyme, Taxus cuspidata erase nitrite been is performed a higher melting. However it is cheap wood neck of biologically active it is cheap the shaking result is a higher melting flower root and of using all of the studies of refresh of frame medical field reported to frequently visits result is of wet liquid to flow down.

oriental oak said neck mill plant dicotyl (Quercusvariabilis) leaves with the dwarf habit in the mill easily recovered and regenerated yellow light. characterized in that. Example oriental oakbell room from the n bit parallel data inputted is used for food consumption of tannin (tannin), sustain, such as containing various ingredients are [...] (quercetin) is very useful even with the existing ways to infuse well food the field and serves to shrinking blood vessels are compressed. Along hemostatic-convergent shell needles and fasting treatment, diarrhea, desulfurization, hemorrhoids including expectoration, analgesic such as has been used in the. On mill for physical properties, growth characteristics, distribution and chemical properties much during the a higher melting using the same but and the processing flow into an in medical field objects' traces to shaking the transfer.

Hereinafter, the present invention more rapidly and to reduce a memory..

The present invention refers to royal azalea extract number. under public affairs including melanin synthetic facilitating composition.

Said composition together it is cheap extract, extract or mixtures thereof oriental oak may further include any.

5 extract royal azalea said composition to 90 weight %, 5 to 90 weight % it is cheap extract 90 to 5 extract oriental oak and includes weight %.

When a wireless type through a wire said, the efficacy of the each component number group containing polyaniline, polypyrrole and polythiophene as the door not number. can occur.

Said extract the ethanolamine, methanol, distilled and mixtures thereof selected from the group consisting of solvent extract, ethanol are preferably used in the form of but 802.11a not one number number.

Dysplasia melanin composition generated by said it comprises a bag counter evidence or aunt prevention or treatment of composition can be used.

Said cosmetic composition composition, or pharmaceutical composition may be ball number by health food.

Said cosmetic composition of the present invention external ointment, based cosmetic, makeup cosmetic, or shaving cosmetic body transferred into cosmetics for can be ball number, examples of based cosmetic said seat belt, lotion, cream, gel, wash containing a crude drug, pack, massage cream, may be such as emulsion, examples of any cosmetic product makeup said foundation, lipstick, eyeshadow, Eyeliner, mascara, oh Eve Low pencil, makeup point can be database or the like is, at cosmetic body soap, liquid cleansing number, bathing number, minced screen cream, may be minced oil, cosmetics for shaving [...] after at, such as shaving cream.

royal azalea said absolute as an active ingredient, an extract, extract it is cheap, oriental oak neck extract or mixtures thereof further in addition to excipients for property or functional materials number, a substance such as a dilution number may include KIPO &.

In one example, said cosmetic composition property for perfume, dye, sterilization number, number antioxidant, antiseptic number, moisturizing number, number incrementally, inorganic salt or synthetic polymer substance can be selected from. May be added in addition to component maintained at formulating component a, number [...], surfactant number, organic pigment, inorganic pigment, organic powder, ultraviolet absorbing number, number adjusting pH, alcohol, wt % number, number of arctium Lappa, a number forward or number can be and so on that can be number.

Addition of other than said active ingredient may be not a formulating component is constrained to prevent, which said purpose of the invention the present primary ingredient within such a range that causes no damaging the and effects, . in.

In addition, said cosmetic composition solution, emulsion water, viscous type, in addition to mixtures may take the shape of,, no bath number used to monitor the art may be number and in one example spray, emulsion, cream, wash containing a crude drug, pack, foundation, lotion, cosmetic liquid, hair cosmetic such as. may be number.

More particularly spray composition cosmetic of the present invention, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, skin lotion, skin [...], skin toner, [...], lotion, milk lotion, the feed it grazes, lotion, nutritional lotion, massage cream, nutritional cream, cream [...], hand cream, foundation, seat belt, nutritional seat belt and may include a type number of.

In one example, of the present invention number type this spray or powder when the carrier components as lactose, talc, silica, aluminum [...], calcium silicate or polyamide powder may be used, in particular of the present invention number an upper electrode is formed on this spray type [...] chloro additionally, propane/butane or dimethyl, such as a propellant may comprise an.

Of the present invention number type paste for, cream or it is a gel when the carrier components as animal fiber, plant fiber, wax, paraffin, starch, estradiol Kant, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or a zinc oxide film can be is used as the.

Emulsion solution or type of the present invention number when the carrier components as solvent, solvating number, or number of [...] is being used, such as water, ethanol, isopropanol, ethyl polycarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1, 3 - butyl glycol oil, an aliphatic ester glycerol, polyethylene glycol or can be fatty acid ester of sorbitan.

Of the present invention number type water as carrier components when the suspension, liquid, such as glycol or propylene ethanol dilution number, ethoxylated ISO stearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan acid esters, such as the suspending number, microcrystalline cellulose, aluminum [...] metadata, bentonite, agar or estradiol can be is used as the Kant.

Active - the interface type of the present invention number number as carrier components when the cleansing containing aliphatic alcohol sulfate, aliphatic alcohol ether sulphate, sulfoalkyl succinic monoesters, isethionates, imidazolinium derivatives, methyl [...], [...] nose yarn, ether sulphate fatty acid amides, alkyl amidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid d ethanol amide, vegetable oil, glycerol fatty acid esters or ethoxylated derivatives it teased can be is used as the.

Said pharmaceutical composition pharmaceutical composition commonly used number jaws suitable carrier, excipient or number may include further dilution number.

In the present invention available carrier, excipient include dilution number or number, lactose, dextran dextrans, sucrose, sorbitol, mannitol, xylitol, Corynebacterium glutamicum cj88, maltitol, starch, rubber acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxy benzoate, profile hydroxy benzoate, talc, magnesium stearate or mineral oil as to the aromatic hydrocarbon KIPO &.

The present invention according to pharmaceutical composition, each number acid according to conventional method, granules number, forward number, number capsule, suspension, emulsion, syrup, aerosols, and the like of oral number type, external number, left number and sterile injection in the shape of a solution can be by aligning number.

When the common filling it be chipped number number number, number extender, coupled number, wet number, disintegrating number, surfactant number number or excipient such as the jaw using dilution number is number. Forward the number number solid for parenteral administration number, ring number, acid number, number granules, capsule number including an, such solid the number number number said compound is at least one or more excipients, for example, starch, calcium cyclocarbonate (calcium carbonate), sucrose (sucrose) or lactose (lactose), of gelatin can be number of seat.

In addition simple number excipients in addition to magnesium stearate, talc such lubricity, is carried out by using an acidulous are also number. Orally liquid suspension include number number number, a content liquid number, number oil, syrup number, including a simple water in dilution number commonly used which, in addition to paraffin a bioliquid number various excipient, for example wet number, sweetener number, number direction, preserving can be include a number.

For parenteral administration in the sterilized aqueous solution the number number, number for non-aqueous, suspension number, number oil, freeze-dried number number, left number is included. Number for non-aqueous, suspension number include propylene glycol (propylene glycol), polyethylene glycol, vegetable oil olive, such as an oil, ethyl [...] the scanning electrode and the ester can be or the like is used as an. Left (witepsol) [...] include number of number, macrogol glyceride, 61 twin (tween), carcass five fingers, does comprising lauric acid, glycerophospholipids can be or the like is used as an oxaliplatin number.

The present invention according to pharmaceutical composition royal azalea absolute as an active ingredient, an extract, extract it is cheap, oriental oak neck extract or mixtures thereof is used in an amount of patient's age, sex, weight or any disease but 42, 0. 001 to 100 mg/kg in, preferably 0. 01 to 10 mg/kg daily for times S101 - S102 1. can be administered. 　

In addition, dose constitutes a route, said pharmaceutical composition, degree of diseases, sex, weight, age can be increase/decrease and the like. 　Thus, any dose constitutes said manner, surface of the present invention. are not limited to range.

Said pharmaceutical compositions RAT, mouse, livestock, human such as the various pathway to mammals can be administered. The all conclusion of the administration of can be expected, for example, oral, rectal or intravenous, muscle, avoid, suction in bronchitis, intrauterine epidural in cerebrovascular or (intracerebroventricular) can be as administration by injection.

Said post-treatment system powder, granules, forward number, capsule, syrup or beverage in the form of can be ball number, said post-treatment system royal azalea absolute as an active ingredient, an extract, extract it is cheap, oriental oak neck extract or mixtures thereof or moderate the sharp taste and smell, and for preparing foods or food-which is used in conjunction with, conventional method suitably according to can be used. For effective ingredient mixing tilted prevented for example a use purpose thereof, according to health or therapeutic treatment of can be determined for.

Health food contained in said extract royal azalea, it is cheap extract, extract neck oriental oak the capacitance effective or a mixture of both said effective pharmaceutical composition the height to the capacity, health and sanitary pad intended to a long is intended to control health or in the case of ingestion of which may be hereinafter said range, active ingredient with a fixing member of the back door number in the aspect of safety because of the absence of said range can be used also in an amount of from at least. secure.

Said a with specific type of health food and free from one number, examples meat, sausage, bread, chocolate, candy current, [...], confectionery, pizza, ramyon, other noodles, gum type, comprising ice cream for dairy number article, various sprocket, beverage, difference, drink number, composite and vitamin alcoholic beverage as to the aromatic hydrocarbon KIPO & number.

Hereinafter, in the embodiment to the present invention through the more rapidly and to reduce a memory.. Just, such in the embodiment by the present invention is limited not.

<In the embodiment 1>royal azalea, number bath it is cheap and oriental oak neck ethanol extract

royal azalea the present invention used in a, oriental oak and it is cheap wonder you as pearl in pearl a steep slope or a neck rim Academic a favorite -75 °C supply pipe an excellent after storage amount and the same corresponding advertisement based on the shown list used, followed by, the third to eo sound case at room temperature.

Dry royal azalea, each neck oriental oakit is cheap and small pieces after 14 to 200g (about 3 cm) 500 ml 95% ethanol at room temperature by mixing the extracted liver 7. Extract filter paper (No. 2, Whatman) are filtered (freeze dryer) after freezing dryer conduit is excited is powder was under trillion number.

<In the embodiment 2>antioxidant activity analysis (review ability erase radical DPPH)

Extract antioxidant activity peak reel Hydra quality 2, 2 - diphenyl - 1 - (2,2-diphenyl-1-secure, DPPH, Sigma, USA) using the embodiment legally DPPH (Monica et al. , Reference 2007). Number in said in the embodiment 1 to which the extract under trillion the dilution at a concentration of 100 ppm 96 well plate( Falcon ^(r), USA)l micro 50 put at each well. And 0. L micro 200 2 mm DPPH solution 30 minutes at room temperature adding reacting a metal salt of enzyme immunoassay method(enzyme -linkedimmunosorbent assay, ELISA, Anthos ^(r), Austria)it was determined that absorbance in 517 nm into reader. The control group in [...] n (Butylated Hydroxy Toluene, BHT, Sigma, USA) using ethanol 95% and factor have been measured by the same method.

As a result, a table, such as 1 royal azalea, it is cheap, oriental oak, mixing (royal azalea ethanol extract: it is cheap ethanol extract: oriental oak ethanol extract, 1:1:1 (w/w/w)) ethanol extract each between about 87, 91, 82 or soldiers contrast positive to 90% BHT and a similar active (95%) been found to have an. Antioxidant activity relative-to-back relationship and at polyphenol content, and menstrual content phenol high part of the metal activating effect is determined.

*: Mg/g powder gallic acid equivalent

**: Mg/g equivalent [...] parses/powder

<In the embodiment 3>polyphenol content analysis

Polyphenol extract knife toe cob - morpholin content (folin-ciocalteu) legally embodiment the (Luis et al. , Reference 2007). L micro 30 gallic acid (Sigma, USA) (1 mg/mL) a mixing and to 3 ml of distilled water 100 micro l folin-ciocalteu (Sigma, USA) reagent then the composition is left for 7 minutes by the addition of 300 micro l of sodium carbonate (sodium carbonate) reacted minutes for mixing and 30. Spectrophotometer UV - then(UV -spectrophotometer,U -[a]x[/a] ^(r), Hitachi, Japan)in 765 nm using of calibration curves and the measured absorbance was created. In said in the embodiment 1 in the same method of powder extract under trillion number of calibration curves and the measuring and absorbance compared to polyphenol extract it was determined that content.

As a result, during said in the embodiment 2, such as 1 table it is cheap ethanol extract, extract and ethanol royal azaleaoriental oak ethanol extract each total polyphenol content 106. 9 mg/g, 252. 1 mg/g or 116. it is cheap cd1a. 4 mg/g, royal azalea, 1:1:1 oriental oak neck ethanol extract 200 (w/w/w) mixture. This solution is poured into a tender 2 mg/g.

<In the embodiment 4>flavonoid content analysis

Davis content flavonoid extract (Davis's) legally embodiment the (Monica et al. , Reference 2007). 0 powder extract under trillion number in said in the embodiment 1. 5g for methanol 1 ml, diethylene glycol (diethylene glycol) applied at 1N NaOH after mixing 10 ml of a 1 ml added. Water bath 37 °C same time whereby reactions can take place in 1 (water bath)UV -spectrophotometer(U -[a]x[/a] ^(r), Hitachi, Japan) 420nmin it was determined that absorbance. The standard material, the third to eo [...].

As a result, during said in the embodiment 2, such as 1 table it is cheap ethanol extract and royal azalea ethanol extract, 33 each flavonoid content extract ethanol oriental oak. 5 mg/g, 74. 4 mg/g or 27. 7 mg/g cd1a. it is cheap ethanol extract and royal azalea ethanol extract, 1:1:1 oriental oak ethanol extract 51 (w/w/w) mixture. Have been shown to 4 mg/g.

<In the embodiment 5>L - waveguide (L-dOPA) mushroom-type using it is sour with the mote but Oh number (mushroom tyrosinase) active capability analysis

it is cheap, royal azalea, oriental oak ethanol extract to confirm the ability of serve an important function in generating melanin so that it shows a it is sour with the mote but Oh (tyrosinase) number has been observed on effects which can direct. The Tyrosinase L - tyrosine (L-dyrk1a) for L-dOPA then the center channel waveguide L - (L-dOPA) a quinone waveguide L - (L-dOPA quinone) .the. Therefore using L-dOPA as tyrosinase substrate in number not so that it helps to select it was determined that a mushroom tyrosinase activity of (Chang et al. , Reference 2007). 0. 1M of phosphoric acid buffer (phosphate buffer, pH 6) 100 micro l l micro 20 extract and a (1 mg/ml, 2 mg/ml) after being mixed with mushroom tyrosinase (2000 U/ml in phosphate buffer, pH 6) added l micro 20 10 minutes in 37 °C reacted. ReactantsUV -spectrophotometer(U -[a]x[/a] ^(r), Hitachi, Japan)it was determined that absorbance in 475 nm using. Tyrosinase activity based on expressions a ring based 4 - trifluoroacdtohpenone derivatives, the 1.

As a result, also, such as 1 L-dOPAroyal azalea and it is cheap ferric oxide as an electron a substrate, when reaction 2 mg/mL oriental oak ethanol extract the each between about 8%, 8% or 20% have been increased. it is cheap, royal azalea, 1:1:1 oriental oak ethanol extract (w/w/w) blend the mushroom tyrosinase activity increased 12% been found to extract ethanol oriental oakit is cheap ethanol extract and are either a homopolymer of processing of a relatively high activity have demonstrated.

<In the embodiment6>B16 melanoma (melanoma) analysis active in a cell,

1. B16 melanoma (melanoma) cell culture

C57BL/6J very cells derived from mouse B16 melanoma [...]oh serum 10% to enable the from hospital a favorite (fetal bovine serum, PBS) containing DMEM 37 °C improves, 0. 5% CO₂good in incubator condition.

2. B16 melanoma using cytotoxicity assay

MTT assays 3 - (4, 5 - dimethyl aminothiazole - 2 - one) - 2, 5 - diphenyl tetra thiazolium bromide (3 - (4,5-dimethylthiazol-2-fermentum yl) - 2,5-diphenyl tetrazolium bromide, MTT) reagent cells after absorbed into the mitochondria succinic dehydrogenase (succinate dehydrogenase) the cup which it rolls up formyl by (formazan) of the material to forming an activity of intracellular accumulation of a mitochondrial, means activity of living cells broadly cell growth by an exemplary measuring a rate is method.

B16 melanoma cells 24 well plate to rationalization on the cell count( 7×10³ cells/well)to 37 °C and the, 0. 5% CO₂incubator in it is cheap then grown on time 24, each extract ethanol oriental oak and royal azalea by concentration (5, 10, 20 mg/ml) to 1 dilution of manufacturing the same are provided to micro l placing at 0. 5% CO₂incubator in good time 72. Culture nor post-incubation plate PBS then triggers number medium which washes the times 1 to MTT (5 mg/ml) a medium per 500 micro l placing at 37 °C l micro 50, 0. 5% CO₂incubator in good time 1. opinion width hour freight dimethyl and triggers number medium after reaction (dimethyl sulfoxide, DMSO) into 500 micro l in the plate shaker (plate shaker) at 15 minutes is melted cells then it was determined that absorbance at a wavelength 540 nm into ELISA reader. The nothing control job offerlifestyle market rate cells showed compared to the control group.

As a result, also the activity of living cells B16 melanoma 2 such as a micro g/mL royal azalea ethanol extract 10, 20, and number 10, 20 micro g/mL mixing treatment group for cell proliferation under the outside method and for circuit controlling isolation gates sequences as signal that do not suffer from the number. 20 micro g/mL B16 melanoma cells royal azalea ethanol extract processing groups 72 on upper and time when maximum 49% cell survival until it has been discovered that the rate. However typically similar differentiation of cells dendritic state (a porous membrane of a single layer-like) shape is monitoring owing to formation of cells. it is cheaproyal azalea and Figure 3, oriental oak ethanol extract of treated cells, contrary to exhibit visual or stimulating or enhancing a cellular change. royal azalea number is shown in Figure 2 and for circuit controlling isolation gates of cell proliferation in the case of control cells when compared to typical resin have demonstrated on cells. it is cheap such a porous membrane of a single layer-like shape, and royal azaleaoriental oak ethanol extract processing group in both concentration increases gradually the c/l splitter. In addition existing in thesis is a porous membrane of a single-like shape differentiation induction cells supervis ion portion confirms a relates the control group positive to results processing into a cell arsenic trioxide in a porous membrane of a single layer-like shape the lower piece is formed to be command it has been confirmed.

royal azalea and it is cheap thus, effectively extract ethanol oriental oak of inducing differentiation of adenocarcinoma cancer cells cells B16 melanoma is determined to.

3. B16 melanoma using analysis ability active tyrosinase

The melanine biosynthesis, Tyrosinase during three phases of purposes as facilitating the. are important for measuring activity of tyrosinase. Thus royal azalea and it is cheap in B16 melanoma, tyrosinase activity oriental oak ethanol extract it was determined that for on influence of each of. B16 melanoma cells steps on or off a cell count 60 ø plate( 8×10⁴cells/well)to 37 °C and the, 0. 5% CO₂, incubator in it is cheap then grown on time 24, each extract ethanol oriental oak and royal azalea by concentration (5, 10, 20 mg/ml) to 1 micro l placing at the dilution 0. 5% CO₂, 37°Cincubator in good time 72. Culture nor post-incubation medium number of cells in a trypsin and triggers containing transferred to tube e - 1% Tryptone X-100 solution and pH 6. 8 PBS mixed with a dissolution buffer (lysis buffer) at 100 micro l in ice box after solubilized into reacted time 1. 4 °C, 10000 rpm 30 minutes in an upper layer of a fire protective centrifugation assay tyrosinase liquid solution analysis protein point, viscosity index, oxidation stability solution (Bio rad, USA) in measuring absorbance, the composition in an equal amount and the bill amount protein. Protein 20 micro g including the total test substance extract and cells 20 micro l is one application of the foregoing is in a 180 L-dOPA after mixing the micro l doesn't have any error frames, at 0. 5% CO₂, 37°Cincubator in the time response 1. It was determined that change extinction in 490 nm after reaction.

As a result, also 4 such as a 20 group out processing extract ethanol neck it is cheap micro g/ml on upper and the royal azalea purchases the passenger to exhibit increased maximum 14% ethanol extract, the neck 10 micro g/ml on upper and 13% increased, which have been found 20 micro g/ml extract ethanol neck oriental oak on upper and the maximum 14%, was shown to be increased. it is cheap, oriental oak neck ethanol extract royal azalea and mixture of the 10 micro g/ml (1:1:1 (w/w/w)) on upper and 7% increase to exhibit purchases the passenger dispersion analysis (analysis of variance, ANOVA) external grudgeit is cheap number mixture result, royal azalea, each extract are either a homopolymer of processing group oriental oak P<0. 05 have been shown to explore significance. Thus it is cheap, B16 melanoma royal azalea and oriental oak neck ethanol extract intracellular tyrosinase affecting activation been determined.

4. B16 melanoma using melanin formation analysis

B16 melanoma cells steps on or off a cell count 60 ø plate( 8×10⁴ cells/well)to 37 °C and the, 0. 5% CO₂, it is cheap then grown on time 24 in incubator, royal azalea, each extract ethanol oriental oak by concentration (5, 10, 20 mg/ml) to 1 micro l placing at the dilution 0. 5% CO₂, incubator in good time 72. Culture nor post-incubation medium e - is stripped off and triggers number of cells in trypsin containing transferred to tube 1% triton X-100 solution and pH 6. 8 PBS mixed with a lysis buffer at 100 micro l into nailed to ice after solubilized reacted time 1. 4 °C, 10000 rpm in an upper layer of a fire protective centrifugation 30 minutes after triggers number liquid the remaining pellets, the third to eo to melanin content reading. Dissolve the melanin 1N NaOH solution in the n bit parallel data inputted measuring absorbance 490 nm, of calibration curves and the melanin (Y=0. 00075X + 0. 0348) using the bill content.

As a result, also in cells B16 melanoma it is cheaproyal azalea and 5 such as a, of extract ethanol neck oriental oak evaluates it with regard to effect results, it is cheap, both extract ethanol neck oriental oak and royal azalea of B16 melanoma cells was effect are. The control group as compared to 5, 10, 20 micro g/ml extract ethanol it is cheap each 39, 81 or 89% increased and an excellent ability to melanin formation is shown to thewater in a manner that renders the royal azalea ethanol extract is identified as 5, 10, 20 each micro g/ml 61, 78 or 98%, was shown to be increased. oriental oak ethanol extract each 5, 10 or 20 micro 14, 58 or 61% g/ml, was shown to be increased. royal azalea and it is cheap, oriental oak ethanol extract of the n bit parallel data inputted increases in concentration dependent fashion processing extract royal tomb the control group compared to extract ethanol neck oriental oak ANOVA statistical analysis results it is cheap group processing alone, royal azalea, oriental oak neck ethanol extraction mixture (1:1:1 (w/w/w)) a number 5 micro g/ml increased enabling the significance of and under the outside it has been confirmed.

<In the embodiment 7>analysing effect of and an epidermal turn mouse

1. Preparing model animal

Positive contrast job and mouse C57/BL6 soldiers F1 week zero 6 mouse (C57/BL6 × BALB) the central model animal for purchase in corresponding advertisement based on the shown list, wonder you a favorite model animal was bred by workability. Animal breeding panel assembly for driving motor 23 ± 2 °C, 35 to 60% relative humidity, 12 time lighting period-free drinking water and dietary under conditions corresponding advertisement based on the shown list to eat 7 7 after undergoing adaptation period between week zero experimental is performed for all the using mouse.

2. it is cheap, royal azalea, oriental oak ethanol extract processing or mixtures thereof

Mouse hairs of the selected portion to the skin of a user first number of rest periods after triggers pink light secured to the skin, and the space between the projections, ethanol extract using cow's milk put him right bar the (0. 2 mL/cm²). On the opposite side of the mouse and the control group the same ballast 60% ethanol, which has the commencement to the skin. Extract processing the n bit parallel data inputted progress months 6 1 day was processing times. Mouse associated with color skin color shift in turn has been observed on periodically.

3. Mouse turn and melanine content reading a bright field human

Complex process of glass, polycarbonate, polytetrafluorethylene, operate within the same process includes must in hair compared to HPLC analysis the points are level melanin can be meta data are compared using analysis light to the length of the light spectral sensitivities that it was determined that melanin content (Lee et al. , Reference 2007). Extract formed on the chip in hair, growing in her breasts spectroscopic light to the length of the light measuring melanin content of the substance was assessed quantitatively lotion, lotion, vitiligo.

Positive contrast soldiers C57/BL6 a test groups F1 mouse (C57/BL6 × BALB) using razor hairs turn then to collect 20 mg per 1 ml of 1M NaOH time 24 and a reacted at room temperature. A500 wavelength both test group and the control group (total melanin) and it was determined that absorbance in A650 (eumelanin). 1M NaOH of eumelanin Melanin and 0 each concentration range. 05 to 0. A by smelting the of 4 mg/Ml of calibration curves and the adjustor and the melanin content the bill.

As a result, also 6 using ethanol 60%, such as the control group when compared to extract ethanol it is cheap, royal azalea ethanol extract, extract ethanol oriental oak, mixture (it is cheap ethanol extract: royal azalea ethanol extract: oriental oak ethanol extract, 1:1:1 (w/w/w)) each processing in which the part defined as black vehicle. capable of confirming the-burn symptom.

In addition, growing in her breasts after application extract hair in spectroscopic light to the length of the light measuring melanin content of the substance lotion, lotion, vitiligo results quantitatively evaluating the, also 7 result content reading of turn mouse, such as 60% when compared to the control group job processing ethanol Melanin eumelanin and shown increased content. When melanin content it is cheap ethanol extract processing groups 40%, 47% royal azalea ethanol extract processing group, 36% oriental oak ethanol extract processing group, royal azalea and it is cheap, oriental oak ethanol extraction mixture (1:1:1 (w/w/w)) representing the dye black corresponding advertisement based on the shown list increased the 39% formed thereon a plurality of holes for content type melaninit is cheap ethanol extract, extract ethanol royal azalea, mixture (it is cheap ethanol extract: royal azalea ethanol extract: oriental oak ethanol extract (1:1:1 (w/w/w)) are each 29%, 29%, 21%, 27%, was shown to be increased. Thus it is cheap and royal azalea, melanin formation extract ethanol oriental oak play a role in a positive manner can be determines that a.

4. Mouse skin through melanin cell observation L-dOPA dyeing

Melanin cells black applied to positive by DOPA to determining presence of cells melanin change according to processing extract position and inserted and obtains the KIPO &. Mouse skin clear it stopped profile or a piece secured to an pH 7. 4 of 3:4: [...] (dihydroxyphenylalanine, Dopa) in a cell when cultured in solutions with dopa-melanin is dyed to black is eliminated. These cells change of waveguide - positive reaction (DOPA-positive). is. Mouse the epidermis is reaction is typically a melanin pigmentation activity occurs in cells.

To this end raid to&black bug point, viscosity index, oxidation stability (Laidlaw & Blackberg) standard procedure for carrying out reactions the skin with clean sheets shown. Such as mouse first separated from the epidermis and the dermis of skin for separating diamine company acet acid ethylene method by cylinder to have (ethylenediaminetetraacetic acid, EDTA) solution. 6 well plate in 2 time [...] micro reacting a metal salt of EDTA solution and (microforcep) separated the coverstock using. Separated mold for about 1 minutes 37 °C and then being washed with saline, 1 L-dOPA time good and placed over the solution. 1 process has been completed L-dOPA 37 °C replacing a solution, good again time 8. Culture nor post-incubation about 1 minutes which washes the saline to the 20 minutes in 10% formalin. 3 minutes after fixing using distilled and clean the 100% ethanol to 95% ethanol (ethanol) and 20 minutes in skin are reacted in a sequentially air was authored in the first client by a number. Finally xylene (xylene) to the car it hangs reacting a metal salt of 2 minutes using a solution of blind sum (cadana blasm) micro is eliminated carefully cover glass falls over her. Then using optical microscope to check upper area of melanin with which an article has been dyed has been observed on cells.

royal azalea and it is cheap, oriental oak ethanol extract through mouse or the like after the processing mold for 5 months observation by separation also results, such as 8, the control group when compared to around follicle cells melanin more observed was capable of confirming the. royal azalea and it is cheap, oriental oak ethanol extract after treatment, 60% ethanol processing group (vehicle) skin observed in hair follicles are not obtainable from melanin cells revealed that more around. From melanin cells owing to the activation of mouse hair follicle the root of the and increased from melanin cells around the already reported bar KIPO &. Thus this result may royal azalea and it is cheap, oriental oak ethanol extract the bag, and thereby inducing the coloring counter evidence is determined.

At least a particular portion of the present invention detail the 25mbps and techniques, homogeneously distributed to be person with skill in the art, only the specifically such and the user makes a one preferred embodiment, the number range of the present invention that are not one will it is apparent that point. Thus, by issuing an ranges are substantial of the present invention claim and their equivalent defined by will the pixels include.