16-02-2017 дата публикации
Номер: US20170042104A1
Автор:
Casper Timothy,
Defelice Charles Philip,
Hallahan David L.,
Ito Bruno Ieiri,
Keeler Sharon Jo,
Kratz Katrina,
Liedgens Markus Michael,
Mather Brian D.,
Meadows Scott,
Nunhez Marcos Luciano,
Perrotto Joseph Anthony,
Picelli Eduardo Da Cruz Maduro,
Prioli Laudenir Maria,
Ragghianti James John,
Whitcombe Christopher C.
Принадлежит:
The present disclosure provides a method for propagating a crop using meristem excision, micropropagation, plantlet formation, plantlet singulation, plantlet transfer to artificial seeds, and planting artificial seeds. Methods are provided for producing and handling the plantlets and materials used to form artificial seeds. The artificial seeds may be used to store plantlets or may be planted to form a crop. 1. A method of planting sugarcane , said method comprising: a. growing excised sugarcane meristem tissue on solid support media in the presence of plant hormones, and', 'b. transferring the tissue from step (a) to liquid growth media in the presence of plant growth regulators; and growing to form the regenerable plant tissue;, '(i) obtaining a regenerable plant tissue comprising,'} [{'sup': 3', '3, 'a. cutting said tissue into smaller segments having a size from about 1 mmup to about 1000 mm,'}, 'b. placing said cut tissue into temporary immersion with liquid medium for at least about 3-10 days, and', 'c. replacing the medium after about every 3-10 days;, '(ii) subdividing said regenerable plant tissue comprising,'} [ i. a cavity of an array structure,', 'ii. a tube-like structure, or', 'iii. a support structure; and, 'a. placing the plant tissue into at least one of the following, 'b. growing said tissue;, '(iii) further growing said subdivided regenerable plant tissue, comprising,'}(iv) forming plantlets from said regenerable plant tissue by growing in media lacking hormones;(v) transferring the plantlets from the structures of (iii) to an artificial seed; a. increased light intensity,', 'b. reduced humidity, and', 'c. contact of plant roots with media supports such as Rockwool, Metro Mix 360®, soil or other material;, '(vi) forming plants from said plantlets, comprising exposing the plantlets to one or more of the following conditions a. introducing said artificial seed structure into a space, and', 'b. transporting said seed structures to a field; and, '(vii ...
Подробнее
