Method for the fluorimetric determination of endotoxins, peptides containing a fluorophore used in the said method and their method of preparation
Номер патента: DE3174109D1
Опубликовано: 17-04-1986
Автор(ы): Michel Monsigny
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Опубликовано: 17-04-1986
Автор(ы): Michel Monsigny
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Реферат: PCT No. PCT/FR82/00003 Sec. 371 Date Sep. 9, 1982 Sec. 102(e) Date Sep. 9, 1982 PCT Filed Jan. 6, 1982 PCT Pub. No. WO82/02382 PCT Pub. Date Jul. 22, 1982.Method for determining endotoxins in a liquid, wherein said liquid is contacted, at 20 DEG -40 DEG C., with a lysate of limule amebocytes and an aqueous solution of a peptide having the following formula: <IMAGE> (I) in which [Pept- is a peptidic hydrophobic chain, n is 1, 2, 3, 4, or 5, B is <IMAGE> being H or CH3, -NH-Ar] is the residue of a fluorescent heteroaromatic amine and X(-) is an anion, the pH of the thus obtained medium is adjusted at 5 to 10 and the heteroaromatic amine thus formed is determined by fluorimetry, and new peptides according to formula (I) usable in said method.
Oxidised phospholipids comprising a fluorophore moiety and use in the determination of the presence of enzymes having antiatherogenetic activity
Номер патента: CA2654743C. Автор: Albin Hermetter,Michael Trenker. Владелец: Technische Universitaet Graz. Дата публикации: 2015-04-07.