Composition for prevention or treatment of thrombotic diseases comprising extracts or fractions of Playtcodon grandiflorum
The present invention refers to synergically by using other medicinal herbs extracts or their fractions diseases which contain as the active ingredient thrombotic prevention of, pharmaceutical composition or for treatment or treatment is directed to a food composition.. Heart disease and cerebrovascular conditions on the 2, 3 the death of a most representative adult is. In particular cardiovascular disorders of heart disease, peripheral vascular blood vessels and center includes of, each and interact with each other between diseases, other diseases structure of a on the WWW. also to developments to the so-group. A drug of treating cardiovascular disease treatment and surgical treatment used simultaneously but, heart failure, hypertension, hyperlipidemia, diseases reconstriction, arrhythmia or thrombosis in the case of is the main drug treatment method. According to IMS Health, cardiovascular therapeutic agent for a disease a scaled up reaction market all types of years 2002 market to drugs 1 occupies a scale about 5 minutes. Society supplied to a first input of a kaoline, and refused the mayor cardiovascular therapeutic agent for a disease is expected to thickness of one half of the thickness. Circulation disorders of thrombus (thrombus) to is with the generation of the-centric in order to generated for preventing and thrombosis is selected from thrombosis is. important in the treatment vascular disease. Thrombus normal hemostatic process (haemostasis) as general outline corresponding pathological to, platelet by coagulation and plasma for purifying flue gases with a high process is prevented from excessively when enabled, starts making transitions can generate a. Prevention and treatment of thrombotic event together with other injections and anti-the drugs, anticoagulants and greatly clause blood platelet zero is classification. Blood coagulation inhibiting thrombus formation cuts off a heparin, to execute anticoagulants such as coumarin of thrombi main constituent, fibrin to decompose to already been formed thrombus for melting thrombosis thrombotic event are solvent is fixed by attaching the Velcro tape used in aid treatment, vascular bleeding systemic or hemorrhages in the site of injury, and thus as chronic administration such as't suitable treatment. The prevention of thrombosis double effective therapeutic agents antiaggregating constitution: known. Platelet having a diameter of at about 2 to 4 micro-slip prevents a Walker from sliding vascular as wavelength multiplexing disklike m to Shanghai vascular site stanched, blood coagulation factor making the clip for thrombus by by to promote solidifying, rapid vascular damage from the main body portion formed of a biomaterial prevent loss of blood has defence. Platelet in intravascular closed however by partial activation of platelets and to adhesive, aggregation, thrombus formation the evolution reaction thrombotic various order for a bit rate to become is inducing diseases. In addition of platelets and to abnormal activation thrombus to form a fine generated by various are thrombotic event as well as arteriosclerosis, hypertension, diabetes and the like adult diseases and expression to barge which found in, platelet evolution reaction and nucleic acid released by the thromboxane, serotonin by such as inflammatory or edema is followed by a. also. Thus having an inhibitory effect on the platelet aggregation, pears, pineapples, jujubes, materials antiaggregating, stroke such as thrombosis of fatal thrombotic and various preventing the recurrence symptoms for treating and prevention. application value thereof. Part of the antiaggregating medicament include, prostaglandin metabolic is suppressed to thereby cAMP [...] generating power, and reduce or remove the platelet in which serves to thereby decreasing the amount of aspirin, indomethacin, such as of non-steroidal anti-inflammatory such as. In particular, platelet aggregation from arachidonic acid of cell membrane aspirin a inducer the jade it recovers sourly, sacrifice a synthetic prostaglandins deacetylase irreversibly inhibiting synthesis of the thromboxane A2 is drug. Platelet the protein synthesis ability since there is no new platelet is appeared the enzyme having platelet agglutination prohibiting effect until about that interval are held on one side of a 10 degree is required. In prescription hospital actually to prevent of thrombosis preventing blood coagulation and 100 mg aspirin per day as of wet liquid to flow down to take 2 mg Warfarin. However most clinical trial of a a gastrointestinal disorder therapy aspirin in. to entrain the (gastrointestinal side effect). Aspirin is administered, it is a a patient group out (heartburn) barrel in myocardial 40% to 5.2, dyspepsia (indigestion), nausea (nausea) been representing symptoms of such as vomiting or, in the case of the control group was 34% to 0.7. Incidence of necrotizing stomach ulcer by ensuring that in a group administration aspirin in addition is 2 or more times higher implying, kidney toxicity (renal toxicity) and hemorrhagic stroke, thereby affecting a to (hemorrhagic stroke), angiotensin converting enzyme inhibitors, may number (angiotensin-converting enzyme inhibitor) are known as HMG-COa reductase interaction side effects of aspirin derived from natural that can replace high-. more important what development clause blood platelet proposal (Alan D. Michelson, MD. , Platelets, Academic Press, Elsevier Science, USA, 2002). While, synergically by using other medicinal herbs ( The inventor is in the, thrombotic derived from natural for developing therapeutic agent for a disease for the purpose of developing and inputs the coordinates results, synergically by using other medicinal herbs extracts or their fractions platelet that inhibit the aggregation activity thrombotic event by useful the prevention or the treatment of disorders can be used and the present invention, is completed. Synergically by using other medicinal herbs which is the object to of the present invention ( It is another object of the present invention as an effective ingredient fraction extracts or their synergically by using other medicinal herbs comprising diseases for preventing or ameliorating electrode 104 is provided under the foodstuff composition. Said end of the, the present invention refers to synergically by using other medicinal herbs ( A term used in the present invention "synergically by using other medicinal herbs ( "It promotes the" a term used in the present invention the, extract obtained by extracting around route.. Specifically, the it promotes the water around route , C1-4 alcohol or a combination of solvent is, preferably contains the extract of ethanol around route can be. I.e., synergically by using other medicinal herbs ground of a dry weight of about 2 to 20 times, preferably about 3 to 5 a of water for 0.5, methanol, ethanol and butanol such as C1-4 polar solvent of the alcohol or about 1:0.1 to 1:10 mixed solvent having mixing ratio for and use as the elution solvent, extraction temperature 100 °C to 20, preferably at room temperature, extraction period during about 1 hr to 4 reduces blood cholesterol, platelet, leucorrhea needle extraction, extraction of such as extracting ultrasonic extraction or cooling reflux method can be degraded and derived using, thrombotic the prevention or the treatment of disorders for the extraction of materials active if method can be used without limitation. Preferably successive extracted times 5 S101-S102 with leucorrhea needle extraction 1 wherein the filtration of the slurry suspension reduced pressure, 20 to 100 °C with rotary thickener vacuum extract filtration thereof, water concentrated under the reduced pressure at room temperature preferably, C1-4 alcohol or a combination of available solvent synergically by using other medicinal herbs oragnic but can be a product obtained, thrombotic event parasitic infestations or for the prophylaxis of of the present invention capable exhibit therapeutic activity, and are not limited to, extract, for extract of dilution liquid or concentrate, and drying the resulting extract a building, or these adjustments a coformulation or purification includes both product. Said useful synergically by using other medicinal herbs, hybrid, the engine various plant variants can be extracted from, for example, root, of the aerial part and, stem, leaves as well as. that can be drawn from an culture plant tissue. Synergically by using other medicinal herbs in one of the present invention in the embodiment 1 has better mouth feeling and ethanol ground after immersed in an time, cooler with reflux extraction device 3 using extracted time (in the embodiment 1-2). The "fraction" a term used in the present invention, various particular constituent in a product from a mixture including constituents separating a specific group or by method a fraction. a product obtained by. Specifically, synergically by using other medicinal herbs fractions THon said route respect ethanol extract with water, hexane, ethyl acetate or a combination of involve the use of solvents and which may be obtained by fractionation, preferably ethyl acetate fraction synergically by using other medicinal herbs ethanol extract may be obtained by.. I.e., synergically by using other medicinal herbs fractions THon of the present invention, after suspension with water it promotes the, hexane, such as ethyl acetate by fractionation involve the use of solvents and nonpolar produces a solvent fraction polarity each solvent fraction can be obtained. Specifically, after suspension in distilled water having extract ethanol route respect , of a suspension of about 1 to 10 times, preferably about 1 to 5, such as ethyl acetate or times of applied non-polar solvents 1 S101-S102 10 times, preferably 2 times to 5 times available a non-polar solvent with the extraction layer, .may be obtained by separation. Additional in addition an ordinary fraction collected light according to (Harborne J. B. Plant Pathology, 1998, 3rd Ed. p6-7,). More particularly, after said route respect ethanol extract in water, ethyl acetate by the application of ethyl acetate and water soluble water soluble fraction can be fractions. Synergically by using other medicinal herbs in one in the embodiment of the present invention by in water extract ethanol, ethyl acetate fractions the n bit parallel data inputted, after the remaining ceramic ball, a fraction are obtained fractions butanol by applying butanol (1-4 and in the embodiment 1-3). Thrombotic fractions THon extracts or their synergically by using other medicinal herbs of the present invention the prevention or the treatment of disorders can be used effectively in. "Thrombotic event" a term used in the present invention the, all diseases generated by thrombus, and by pushing a button to decide the means, in particular by thrombus, and clogging is calculated if both blood vessels are. all diseases generation system and the method. Said cause of diseases at slow blood flow, excessive coagulation, complex or, alone, is or the like, the vascular lesion and the like acts in the manner of developing a but are known to be, is at. Said thrombotic diseases include thrombosis, hypertension, stroke, cerebral infarction, angina pectoris, myocardial infarction, arteriosclerosis, closed peripheral arterial tone fibrosis, fibrosis vein occlusion elongated, central retinal vein occlusion fibrosis, waste thrombosis, cardiac arrhythmias thrombosis, [...] thrombosis, brain sinus thrombosis, cerebral arteriosclerosis, heart disease, ischemic, two internal bleeding , aneurysm, atherosclerotic thrombosis, neural anger symptoms is to, and a platelet aggregation it contains due to thrombosis, hypertension, stroke, cerebral infarction, angina pectoris, myocardial infarction, arteriosclerosis and ischemic but may include, is at. "Preventing" a term used in the present invention the, said inhibiting activity of platelet aggregation in the composition by other compounds are difficult thrombotic. behavior all or delays. "Treatment" a term used in the present invention the, said inhibiting activity of platelet aggregation in the composition by thereby monitor thrombus diseases or improving the symptoms by beneficial. behavior all easily changed. Synergically by using other medicinal herbs extracts or their fractions of the present invention including pharmaceutical compositions commonly used for making pharmaceutical compositions suitable carrier, diluent or excipient may further comprise the. The, said a compositions the water content fraction extracts or their synergically by using other medicinal herbs the first substrate are assembled but not limited to, relative to the weight the total composition weight % to 20 weight % to 0.001, preferably 0.01 weight % to 10 weight % may comprise an. Each pharmaceutical compositions said purified liquid according to conventional method, bolus, masked powders that are to be, granules number, number capsule, suspension number, content liquid, emulsion, syrup number, in the sterilized aqueous solution, non-aqueous solvent, number suspension, emulsion, a suppository and a frozen desiccant selected from the group consisting of either may have formulations of, polyethylene oxide derivatives, chitosan and heparin can be a variety of formulations. Formulated filling common when the number, number extender, coupled number, wetting, disintegrating, or of a thinning agent surfactant is formulated excipient. Solid medicinal preparation is finished, the perfume ingredient for oral administration, bolus, masked powders that are to be, granules number, capsule number including an, such solid formulation contains at least one compound at least one or more excipients number for example, starch, calcium carbonate, sucrose (sucrose) or lactose (lactose), is prepared of gelatin. Furthermore, simple number magnesium stearic acid in addition to excipients, such as talc is used lubricants provide. Liquid formulations for parenteral administration include suspension number, content liquid, emulsion, syrup number, including the additive and water as the diluent a simple commonly used which, in addition to paraffin a bioliquid number various excipient, for example wetting, sweetener, aromatic, and preservatives may include. Agents are for parenteral administration in the sterilized aqueous solution, non-aqueous solvent, number suspension, emulsion, freeze-dried preparation, left system may be included. Non-aqueous solvent, suspension solvent include propylene glycol (propylene glycol), polyethylene glycol, vegetable oil olive, such as an oil, ethyl [...] such as injectable esterase can be is used furnace, such as a flash. [...] include base left proposal (witepsol), macrogol glyceride, twin (tween) 61, carcass five fingers, does comprising lauric acid, glycero gelatin can be or the like is used as an. Effective pharmaceutical composition of the present invention can be administered in an amount. In the present invention used a term "pharmaceutical effective amount" the, applicable to medical treatment through a rational will benefit/risk ratio a unit, device and method to a sufficient amount meaning, object types and the level capacity effective in the severity, age, sex, type of diseases, drug action, sensitivity to drugs, administration time, route, and discharge ratio, therapy period, the inhaler includes a mechanism to open signal by using an elements and other medical well-known to the field can be determined according to factors. Useful when administered therapeutic agent individual composition of the present invention or with additional therapeutic agents can be administered from therapeutic agent of the existing method can be sequential or simultaneous administration. Single or multiple and. can be administered. Least side effect without considers both the element a starting switch enables the maximum amount of administration of a amount are important, by one skilled in the art can be the storing apparatus. The preferred dosage is composition of the present invention, base lines of patients' conditions and body weight, degree of diseases, drug form, route, according to and for a duration, grinded, kneaded with baking powder, burn once per day administration may administration, may be loaded with administration divided times. Said treatment of thrombotic event composition the purpose is to if an individual which is not particularly limited in, and unbreakable is. applicable, whether they. For example, simian, two, CAT, rabbit, [...] , RAT, mouse, bovine, amount, porcine, nonhuman animals, such chlorine, human, algae and fish as may be used in alternatively the, conclusion of the administration of conventional homogeneously distributed scheme if free, . includes a method. For example, oral, rectal or intravenous, muscle, avoid, intrauterine epidural or cerebrovascular injection can be administered to the patient by. In hot-water extract route respect one in the embodiment of the present invention extract ethanol than as well as bacteria in having potent inhibiting activity of platelet aggregation by strong (also 1). In addition said ethanol in fraction ethyl acetate extract, containing a large amount of saponin known to be active platelet aggregation than butanol fraction by stronger (also 2). Therefore, thrombotic fractions THon extracts or their synergically by using other medicinal herbs of the present invention the prevention or the treatment of disorders can be and is thus useful for the. In addition the present invention refers to, synergically by using other medicinal herbs extracts or their fraction as an effective ingredient including thrombotic event for preventing or ameliorating provides a food composition. Synergically by using other medicinal herbs said, their extract, fraction and a thrombotic event to said taught equal. The "improved" a term used in the present invention, as an effective ingredient fraction extracts or their synergically by using other medicinal herbs including composition potent inhibiting activity of platelet aggregation preventing or by thrombotic event the treated individual invention and suspicious symptoms of all, to advantage or is circulation promoted. behavior. Specifically, of the present invention extracts or their fractions thrombotic event prevention or amelioration food composition intended to. may be included. Type of food of the present invention with specific't limitation. Said synergically by using other medicinal herbs extracts or their fractions can add examples of food meat, sausage, bread, chocolate, candy current, [...] , confectionery, pizza, ramyon, other noodles, gum type , ice cream containing a dairy product, various sprocket, beverage, difference, abundant, or complex vitamin alcoholic beverage and, in the conventional may include both food, the food products made for feed by using includes. In addition to said number nutritional various food composition of the present invention, vitamin, electrolyte, flavor number, colored number, [...] and salts thereof, alginate and salts thereof, organic acid, protective colloidal thickener, pH modulators, stabilizer, preservative, glycerin, alcohol, such as agent carbonate used carbonated beverage can. Otherwise natural fruit juice, fruit juice beverages and vegetable beverage for the production of can matrix shape. Furthermore, the food according to said purified liquid of publicly known manufacturing method, granules, powder, capsule, liquid solution and also films to form solid ring can be produced. Furthermore, in conventional flavor number or natural carbohydrate or the like can be includes additional components. Synergically by using other medicinal herbs extracts or their fractions of the present invention in food additives signal transmitted from the video signal input, said extracts or their fractions directly it has been deposited by either adding other food or components and may be used in conjunction with, conventional method suitably according to, use can be made of,. Of active ingredient mixing amount according to the use purpose can be determined for. Said food additive appearance, flavor, tissue or storage for the purpose of improved a small amount the product to which normal acids, are included in an amount indicating which intentionally, improved quality of food, integrity-convex is formed by an or symbol as well as substantial and vegetables nutrition for the purpose to increase a value is used, .. The, food the hygienic law calls and number 2 number 2 bath as and defines a main, , the food product contains all processing or preserving the foods, mixing, impregnated, other method can be the material used for the in. Synergically by using other medicinal herbs extracts or their fractions THon of the present invention long mugwort derived from natural on used as not fall dead in spite of the side effects, as described above, has potent inhibiting activity of platelet aggregation, synergically by using other medicinal herbs extracts or their fractions said thrombotic prevention of including of the present invention composition, treating or the improvement of the can be useful. route respect (A) also Figure 1 shows a heat/ethanol extract collagen-induced platelet aggregation (B) and, which display inhibitory effects on platelet agglutination prohibiting effect of agricultural extract ethanol synergically by using other medicinal herbs by is graph indicative of the analysis result. Also Figure 2 shows a (A) (B) effect and induction platelet aggregation of butanol fraction route respect synergically by using other medicinal herbs ethyl acetate fraction of collagen and arachidonic acid induced platelet against aggregation in inhibitory effect of agricultural by is graph indicative of the analysis result. Also Figure 3 shows a an increased collagen route respect ethyl acetate fraction of PLCγ 2, compounds and methods for modulating expression of AKT phosphorylation of p47 and analysing effect is indicative of the result. Hereinafter, and/or at least two different and in the embodiment to the present invention. described S406. However, a and in the embodiment examples of the present invention relate of the present invention range for only limited not. In the embodiment 1: of and it promotes the preparation of In the embodiment 1-1: synergically by using other medicinal herbs heatof processing for extracts of Synergically by using other medicinal herbs monitoring apparatus 50 g paste has better mouth feeling and 1 time 1 ℓ with water after immersed in an, herb large male (Daewoong Extractor DWP-5000M, Incheon, Korea) 4 using extracted heat time. Heat which is then subjected to reverse phase, followed by freeze-drying, concentrating it under reduced pressure are obtained for 100 g synergically by using other medicinal herbs the hot-water extract. In the embodiment 1-2: synergically by using other medicinal herbs ethanol of processing for extracts of Synergically by using other medicinal herbs monitoring apparatus 50 g 1 1 ℓ to ethanol paste has better mouth feeling and after immersed in an time, cooler with reflux extraction device 3 using extracted time. Ethanol which is then subjected to reverse phase, the followed by freeze-drying, concentrating it under reduced pressure are obtained for 100 g extract ethanol synergically by using other medicinal herbs. In the embodiment 1-3:it promotes the manufacturing fraction of ethyl acetate (EtOAc) An electrochemical method for said in the embodiment 1-2 synergically by using other medicinal herbs ethanol extract followed by in water of 2 ℓ of 100g, 2 ℓ of ethyl acetate (EtOAc) are mixed together ethyl acetate dissolved separated the layer. A 5 said process after concentrated under the reduced pressure are obtained for 10.4 g ethyl acetate fraction. In the embodiment 1-4:it promotes the of manufacturing fraction (BuOH) butanol Value fraction of said in the embodiment 1-3, contemplate the remaining (normal butanol) 2 ℓ-butanol n n-butanol dissolved by mixing the separated the layer. Said process after a 5 n concentrated under the reduced pressure are obtained for (BuOH) 10.7 g fraction-butanol. Experiment 1e.g.: it promotes theaggregation of cells platelet of and fractionation treatment of osteoporosis inhibitorseffect analysis It promotes the alcohol to the glycolide monomer at said in the embodiment and fractions thrombosis skin tones in order to identify a the effect, of animal model [...] Born as blocks, platelet from method (turbidimetry method) (Born GV, Cross MJ. The aggregation of blood platelets, J. Physiol 168 : 178-95, 1963) educational puzzle kit ability platelet aggregation according to (aggregometer, Chrono-Log Co. , Havortown, Pa, USA) aggregation of platelet cells using human power by operating all systems by inhibiting effect of treatment of osteoporosis. It promotes the alcohol to the glycolide monomer at said in the embodiment (hot-water extract) W20 or fractions THon, E20 (ethanol extract), (butanol fraction) E20-BuOH, E20-EA was referred to as (ethyl acetate fraction). First, took platelet from animal model. Male rabbit (NewZealand white rabbit: Sam-Tako Animal Co. , 25th which, pentoxide) a 24 °C temperature, relative humidity 55% with water in freely feed to easily wash the sufficiently can be taken was domesticated companion. Sampled, blood from rabbit's ear artery, 0.15% contrast blood samples (v/v) volume ratio of anticoagulant citrate dextrose (anticoagulant citrate dextrose, citric 0.8%, tree sodium citrate 2.2%, dextrose 2%(w/v) composed) to said an excellent blood gassed up. Furthermore, the centrifugation 10 minutes 230 × g in a plasma enriched platelet (platelet rich plasma, PRP), obtains and makes, said (PRP) a plasma enriched platelet 15 minutes in 800 × g again precipitating the platelet centrifugal separator. Precipitated platelet the 0.35% SA and a 0.4 mm EGTA (ethylene glycol bis (β-amino ethyl ether)-N, N, N ', N' (N ')-ethylenedaiminetetra acetic acid) a buffer solution including Hepes (137 mm NaCl, 2.7 mm KCl, 1 mm MgCl2, 5.6 mm glucose and 3.8 mm Hepes, pH 6.5) was washed in. Said platelets are flushed back to a buffer solution (pH 7.4) which comprises suspending said Hepes, 4×108 cells/Ml, the third to eo regulated so as to experiments. Are flushed said agglomerating in the platelet suspension (platelet rich plasma) 37 °C in educational puzzle kit exudates and applies a tactile sensation is set in agitating while good in 1,000 rpm. Synergically by using other medicinal herbs (W20) produced in said in the embodiment and a hot-water extract, synergically by using other medicinal herbs ethanol extract (E20) and synergically by using other medicinal herbs ethanol extract of each 50 micro g/Ml (E20-BuOH and E20-EA), 100 micro g/Ml, at a concentration of 300 micro g/Ml or 500 micro g/Ml platelet said added to culture. The control group at the same extract and distilled water and the salt by stably added a dimethyl sulfoxide (DMSO). Furthermore, 1,000 rpm is set in agitating while 37 °C in 3 minutes culturing the cell line in the, platelet aggregation inducing substance-collagen (g/Ml micro 5) or arachidonic acid (100 µm) each platelet aggregation induced by adding. Extent to which agglomerated platelet aggregometer (Chrono-Log Co. , USA) measured using corresponding advertisement based on the shown list, showed to 2 and 1 also result. As a result, excellent than hot-water extract extract ethanol synergically by using other medicinal herbs efficacy and of platelet inhibition been cosmetic products inhibitor concentration dependent fashion (also 1), saponin including the great induction platelet aggregation fractions THon butanol synergically by using other medicinal herbs while efficacy, it does not contain a high saponin ethyl acetate fractions THon induced arachidonic acid collagen and a platelet against aggregation in concentration dependent excellent efficacy by inhibitors (also 2). Experiment 2e.g.: blotsblot analysis Synergically by using other medicinal herbs ethyl acetate fraction of antiaggregating active in order to test, PLCγ 2, AKT phosphorylation of p47 and it was determined that inhibiting activity. PLCγ 2 as a without side effect antiaggregating, p47 or AKT phosphorylation of the inhibiting activity is known and art. Protein extract buffer (50 mm Tris-HCl (pH 8.0), 5 mm EDTA, 150 mm NaCl, 1% NP-40, 0.1% SDS, 1 mm PMSF, and one protease inhibitor cocktail tablet (Roche, Germany)) proteins in the cell by using centrifugal separator extracts the number of complete (10,000 ×g, 15 ingredient, 4 °C) the protein extract is obtained. Protein concentration a BCA protein assay kit (Pierce, IL) to quantitatively using SDS-PAGE. 10 micro g protein samples for the sample buffer (100 mm Tris-HCl, 2% SDS, 1% 2- distant cop toe ethane this year , 2% glycerol, 0.01% bromophenol blue, pH 7.6) and a after mixing the heating (100 °C, 5 ingredient) modified to synchronize the, 10% polyacrylamide gel electrophoretic section. Electrophoretic the Mini protean 3 Cell by cylinder to have (Bio-Rad, CA). The isolated protein gel (Whatman, Germany) carbonyl-, nitro-, a cellulose film the n bit parallel data inputted transmitted to Ponceau S dyeing protein transfer and a drop protein amount has been confirmed. After this carbonyl-, nitro-, a cellulose film to block buffer (10 mm Tris-HCl, pH 7.5, 150 mm NaCl, 0.1% Tween 20, 3% nonfat dry milk) 2 and is divided into blocks by 1 time difference antibodies (dilution drainage, 1:1000; Cell Signaling Technology, Inc. , MA) was incubating the and a. The, said 1 collagen antibodies difference the increase in platelet activating signaling are known as HMG, joining a PLCγ 2, AKT antibodies that specifically bind to p47 and (Cell Signaling Technology, Inc. , MA) the first voice portion out of an. 1 difference antibodies as substitutive film to block buffer 3 times 10 minutes and, after washing the 2 difference antibodies (1:2000) and a 1 was incubating the time. After, blocking buffer 3 times 10 minutes and, after washing the SuperSignal West Femto Maximum Sensitivity Substrate with the (Pierce, IL). The fluorescent signal LAS-3000 luminescent images analyzer (Fuji Photo Film Co. , Japan) the n bit parallel data inputted detected by using the, band density it was determined that using Multi Gauge software version 3.0 (Fuji Photo Film Co.). As a result, untreated fractions ethyl acetate synergically by using other medicinal herbs the control group in the case of, PLCγ 2 by collagen, active AKT phosphorylation of p47 and have been increased. While, when the treating fraction ethyl acetate synergically by using other medicinal herbs said fraction dependent upon concentration of PLCγ 2 in, phosphorylation of AKT p47 and has allowed the marked reduced (also 3). The synergically by using other medicinal herbs ethyl acetate fractions antiaggregating active or not (platelet aggregation inhibitor) suggesting a.. The present invention refers to synergically by using other medicinal herbs extracts or their fractions diseases which contain as the active ingredient thrombotic prevention of, pharmaceutical composition or for treatment or treatment is directed to a food composition.. Synergically by using other medicinal herbs ( Deleted Deleted Deleted Deleted Deleted Synergically by using other medicinal herbs ethanol extract obtained by fraction ethyl acetate ethyl acetate fractions diseases which contain as the active ingredient, hypertension, angina pectoris, thrombosis, myocardial infarction, and arteriosderosis selected from the group consisting of thrombotic event food composition for treatment or prevention. Deleted Deleted Deleted Deleted Deleted
