COMPOSITION FOR REGULATING BLOOD GLUCOSE COMPRISING EXTRACT OF PERILLA FRUTESCENS SPROUTS

The present invention refers to a composition for blood glucose level in order quartz tube including extract are disclosed.

For maintaining homeostasis of blood plasma is adjusted hormone and autonomic is solved to could be bonded each other. I.e., per the insulin is to lower blood glucose promoting and using, as the glucose in blood glucose to promote synthesis methods to increase between the writing base car it boiled glycogen, epitaxial general, glucocorticoid also raise blood glucose levels.

In addition, in autonomic sympathetic nerves decreases blood glucose level, insulin secretion 4. unavailability withoout wishing to be bound to reduce the blood sugar level.

On the other hand, diabetes (diabetes mellitus) or how much insulin secretion function obtains the same is of normal metabolic disorders, the method characterized by increased concentration of glucose in the blood, urine glucose in blood glucose levels due to various conditions and signs for causing the discharge to be coated.

Order quartz tube of the present invention said quartz tube sprout extract agent, ethanol extract or be a sprout quartz tube main extract sprout quartz tube.

A main order quartz tube of the present invention said sprout extract quartz tube can be extract.

Of the present invention said at least two blood glucose level control, said order quartz tube extract raw material after washing and dehydration order quartz tube, then drying and ground, said quartz tube order excellent fertilization solvent after mixing, extraction (1 difference extraction), through the process of the series such as filtration and cream, can be obtained.

Of the present invention said at least two blood glucose level control, said extract 2 times 1 obtained by the extraction process to order said quartz tube tea extract solution by mixing 2 and tea extract solution filtration and cream such as through the process of the series, can be obtained.

Of the present invention said at least two blood glucose level control, said solvent is water when the quartz tube sprout agent, said beverage exhibits high sprout quartz tube when ethanol solvent, solvent in addition said main main sprout extract can be obtained when the quartz tube.

Of the present invention said at least two blood glucose level control, said composition based on the total contrast, said quartz tube order extract 0. 1 - 10% By weight, preferably 0. 5 - 5% By weight, more preferably 1 - 1. 5% By weight can be a.

Of the present invention said at least two blood glucose level control, said composition food, pharmaceuticals or a cosmetic can be.

Of the present invention said at least two blood glucose level control, said food product may be a health food.

Of the present invention said at least two blood glucose level control, said functional sausage be a health food.

Of the present invention said at least two blood glucose level control, said organic acid-containing food, phosphate, antioxidant number, car number de-phosphorus, dextrin, glucose, sugar and brush expense [thol with addition of further comprises at least one number selected from the group consisting but, limited to are not correct.

Of the present invention said at least two blood glucose level control, said organic acid has a citric acid, after end it buys, adipic acid, lactic acid or malic acid may be, said phosphate is sodium phosphate, potassium phosphate, acid pyrophosphate or phosphate (phosphate polymer) and can, said antioxidant number is polyphenol, catechin, alpha - tocopherol, rosemary extract, extract, chitosan, tannic acid or phytic acid such as natural antioxidant handler number, limited to are not correct.

Of the present invention said at least two blood glucose level control, said food number may be solid, powder, granules, positive number, can be a capsule or liquid form, limited to are not correct.

Of the present invention said at least two blood glucose level control, said pharmaceuticals be a pharmaceutical composition for preventing or treating diabetes.

Of the present invention said at least two blood glucose level control, said pharmaceuticals of number type are granules number, lemonade number, acid number, number syrup, liquid number, X number, number l [lik it stands, flow X number, oil number, suspension number, number before, needle number, positive number, a main number, capsule number, number [...], ring number, number COOM, lotion number, candle [...] number, aerosol number, number ointment, adhering number, pasta number, catadioptric plasma number, cream number, number oil, paste number number, number 35b for aqueous, lyophilized number number, number left, scanning can be a number such as, limited to are not correct.

Of the present invention said at least two blood glucose level control, said pharmaceuticals is detected by a reduction in conventional manner can be according to oral administration.

Of the present invention said at least two blood glucose level control, said number may be manufactured of cosmetic, nutritional skin lotion, massage cream, encapsulated into, pack, gel, essence, lipstick, make-up base, foundation, lotion, ointment, gel, cream, cleansing, number cleansing, soap, shampoo, rinse, treatment and cosmetic liquid coming in can be a, limited to are not correct.

Of the present invention said at least two blood glucose level control, said cosmetic aqueous vitamin, planetary vitamin, peptide polymer, polysaccharide polymer, can the [su dizzily, normal lipid are also provided, according to the widely publicly known techniques for conventional bath 1308. number hereinafter.

Of the present invention said at least two blood glucose level control, as well as a cosmetic in addition said component said cosmetic agents commonly used additive number, e.g., vitamin, amino acid, protein, number surfactants, emulsifying number, perfume, dye, stable number, antiseptic number, number antioxidant, UV blocking number, number selected from the group consisting of adjusting pH and chelating number further comprises at an add-on number but, limited to are not correct.

Hereinafter of the present invention content number attainments, and experiments in the embodiment through a could be generally described as follows. However, the present invention they are for more detailed only, of the present invention scope of the invention defined by them are not correct.

1>Attainments<number (number order quartz tube main extract bath)

Quartz tube sprout cleaning water after washing 3 times, dehydrator in 11 minutes and subsequent to 900 rpm, in 45 °C while maintaining his 48.

Said dried quartz tube sprout power mill (14 mesh) using crushing, extracting said quartz tube order into the finely formed thereon, followed by 5 hours in 70% 10 times weight of crushed quartz tube order contrast 70 °C extracted from the main.

Said extract 1. 5 Kg/cm² Hereinafter herein of using pressure of 600x600x30 mm after filtering, 60 °C, then 30 [...] concentrating under reduced pressure, followed by positive number 2 times weight of said concentrate contrast for the last 50 [...][...] 30 easy to periodical repetition number 3 with respect to the concentrating.

Then, 72 hours after freeze drying under vacuum 600 mTorr hereinafter, power mill (50 mesh) order number extract powder was prepared by quartz tube using high pressure liquid coolant.

2>Attainments<number (number order quartz tube main extract bath)

Quartz tube sprout cleaning water after washing 3 times, dehydrator in 11 minutes and subsequent to 900 rpm, in 45 °C while maintaining his 48.

Said dried quartz tube sprout power mill (14 mesh) using crushing, extracting said quartz tube order into the finely formed thereon, followed by 5 hours in 70% 70 °C 10 times weight of crushed quartz tube order contrast main reservoir storing the production of tea extract solution was a 1.

Extracting said 1 difference remains on the extractor 10 times crushed paulownia tree a quartz tube order followed by 70% weight of a main 70 °C 5 in contrast initial quartz tube order 2 tea extract solution obtained by extracting a difference time 2.

Said tea extract solution is uniformly mixed with the tea extract solution obtained in 1 and 2 mixed extract 1. 5 Kg/cm² Hereinafter herein of using pressure of 600x600x30 mm after filtering, and then concentrating [...] 30 60 °C under conditions of temperature and pressure, said concentrate 2 times 30 easy to machine and switching number number 3 is put in the contrast weight [...][...] concentrating end 50 with respect to the periodical repetition.

Then, 72 hours after freeze drying under vacuum 600 mTorr hereinafter, power mill (50 mesh) order number extract powder was prepared by quartz tube using high pressure liquid coolant.

3>Attainments<number (number of beverage exhibits high order quartz tube bath)

70% Ethanol 70% as a solvent instead of in the range of 0.1 other than a main, high pressure liquid coolant to extract powder was the same manner on said number 2 attainments embodiment number order quartz tube.

4>Attainments<number (number of order quartz tube agent bath)

70% As a solvent instead of in the range of 0.1 other than a main chuck, said number 2 attainments easy and high pressure liquid coolant to extract powder was number order quartz tube embodiment.

Example 1><experiment (analyzing characteristic of quartz tube order extract)

In a number of high pressure liquid coolant system of said number 2 main extract e phosphoric acid 70% order swans attainments, residual ethanol concentration, measured moisture content and have been shown to means of table 1.

The, said rosemary acid using HPLC measurements of measured conditions have to table 2, said residual ethanol concentration using HPLC conditions such as for table 3 were measured.

In addition, particle size analyser grating HELOS particle size measured by use of a core group of reinforcing yarns have Korean said tag, said moisture content (%) pre-heating the sample to a weighing dish to clause quantity grudge 5 g 5 105 °C drying time, about 30 minutes to 1 weight decimation kater expressions according cool and were measured.

Example 2><experiments (extract clinical efficacy evaluation before blood glucose level in order quartz tube)

1. Blood glucose level in level in assessing the efficacy

(1). Therefore solution enzyme inhibitory activity

Swells and drug therapy applied to treatment of diabetes type number 2, mainly in the case of drug related enzymes to rapid rise and decomposition per billion blood glucose number number billion, can reduce the use of water-soluble per the start of utilizing other.

In the case of complex from a drug, such as colic and diarrhea is used as new therapeutic number are desired. Α a-glucosidase α a-amylase is contained and final stage for conversion of glucose in dietary carbohydrate source of process gases as, inhibiting number is a source of carbohydrate, a direction to retard absorption increase blood glucose levels could be bonded each other.

In the experiment, 20 μl sample α a-glucosidase inhibitory activity by each concentration measurement is on α-a glucosidase, 40 μl, 0. 5 M phosphate buffer (pH 6. 8), By reacting a 15 minutes in 37 °C by mixing 20 μl, 4 μl applied in 10 minutes (pNPG) 30 mm p a-nitrophenyl a-α-a D non-glucopyranoside value of 28 to 37 °C, 0. 1 M sodium carbonate 20 μl reaction is an integer in measuring absorbance 405 nm have, [...] protease activity according to equations 2 change in the bill.

In addition, each concentration measurements of each sample 10 μl α-a Amylse inhibitory activity on α-a amlyase (6. 25 U/mL) by adding 90 μl enzyme solution after reacted at 37 °C, DNS (3. 5 A-dinitrosalicylic acid) solution then pronounced color enzymatic reaction, measuring absorbance at 540 nm have, [...] expressions 2 protease activity according to said change in the bill.

On the other hand, concentration 50% enzyme activity for inhibiting billion number order quartz tube extract concentration (IC₅₀ ) Precursor to, as a result 0. 67, 0. 23 Μg/mL billion number found in 50% efficacy has been confirmed (2 also).

(2). Insulin signaling activity inducer efficacy analysis

Insulin receptor expression bulb 3T 3 - L1 differentiation of adipose cells of inducing insulin number fat bulb 3T 3 - L1 differentiation is included in vitro differentiation induction medium processing induced differentiation of fat cells.

Fusion (confluent) cells to culture medium after replacing the state (0 and inducing differentiation of uncovered) induction, cell differentiation induction and 10% serum (FBS, Gibco) included in the DMEM culture after 2 small [thay to each exchange medium (Gibco) was 2. Instead of inducing differentiation of adenocarcinoma of fat by processing order extract and quartz tube to insulin, glucose intake utilizing the assay kit (Sigma non-Aldrich) were measured.

Adipose glucose in insulin signal transduction by moving glucose transporter -4 taken cytoplasmic membrane (GLUT4) effected. Muscle tissue taken GLUT1 and GLUT4 glucose acts primarily involved in fat cells is known.

Corresponding with a predetermined length from the fat cells via glucose triglycerides which is a precursor to create and glyceraldehyde-a 3 a-phosphate, converting glycerol to dihydroxyacetone-a phosphate users can communicate back to be coated. Fatty acid biosynthesis in turn is in combination with the neutral fats to be coated to form a fat within cells.

The, quartz tube [...] of glucose into fat cells differentiate into fat cells inlet neutral fats for irradiating said number 2 number in order of relevancy accumulation attainments high pressure liquid coolant extract a quartz tube 10, 50, 100, 200, or 400 μg/mL glucose intake analysis 3T 3 - L1 was fat salvation of the world gun at a predetermined concentration.

Analysis result, quartz tube order according to an extract 2 a-deoxy-a D non-glucose have increased unsaturated fatty acid content in non-verify, quartz tube resulting in his car and facile intake glucose in order extract fat progenitor cells (of Figure 3 A).

In addition, fat 3T 3 - L1 expression in progenitor cells through expression of proteins was compared immune [...] GLUT4 degree. Result, GLUT4 concentration dependence of protein expression but not identified, increased from 50 μg/mL (of Figure 3 B) has been identified.

(3). Pancreatic beta cell protecting capability analysis

Pancreatic beta cell protecting ability to analyze, utilizing his (RINm5F) Rat pancreatic β-a cell line. RINm5F cells (5 x 10⁶ Cells) by treating time behind the queue hemp cloth which is [syen grudge order quartz tube to extract concentration 1, a cytokine mix (IL-a 1 beta, IFN-a γ, TNF a-α) was 24 hours. NO is generated on the auxiliary line (nitrite) (nitrate) is oxidizable to a nitrate, nitrite level in culture supernatant was.

In addition, protein expression of the [wey the [su it shook off through analysis was involved in generating NO iNOS [pul the law. RINm5F cells extract cytokines after processing order quartz tube, ice a-cold lysis buffer (50 mm Tris, pH 7. 4, 1% NP40, 150 mm NaCl, 0. 25% Sodium deoxycholate, 1 mm phenylmethanesulfonylfluoride, 10 μg/mL leupeptin, 10 μg/mL aprotinin) then to acid-fast bacteria (lysis), 12,000 rpm was taken in 10 minutes centrifuging the liquid stream. Sodium dodecyl sulfate-a polyacrylamide gel electrophoresis (SDS-a PAGE) iNOS primary antibody (Abcam, 1:2, 500) and PVDF film after information of the horseradish peroxidase non-conjugated secondary antibody (anti-a rabbit IgG, SantaCruz) on with respect to the reaction product.

5 Result is also shown. RINm5F cells cytokines processing result, NO content analysis of auxiliary line is about 8 have increased doubling compared in nothing control nine verify, said number in a quartz tube order number 2 high pressure liquid coolant attainments extract 10, 50, 100, or 200 μg/mL is pre-treated with concentration of result, from 10 μg/mL concentration effective in generating significant NO billion shows number has been, in a concentration of at least 100 μg/mL-capturing do not process the opening completely inhibiting the cytokine (complete inhibition) has been analyzed.

In addition, generating NO (signaling molecule) employed for confirming the result signal molecules involved in iNOS expression billion number, number 10 μg/mL concentration effects capable of significant billion (4 also).

(4). Neoplastic synthetic pathway (Gluconeogenesis pathway) glucose analysis

HepG2 cells in human hepatocellular carcinoma cell lines (human hepatoma cell line) in a medium DMEM 10% a-FBS(Gibco) on antibiotic number obtained by adding 37 °C, 5% CO₂ Culturing conditions, each one was 3 - 4 involve passaging. HepG2 cells 24 hours before serum free a-DMEM medium in order quartz tube generating glucose extract by culturing cells rest periods for entering her. Quartz tube in sprout (gluconeogensis signaling) protein in said number 2 to analyze glucose by neoplastic composite signal high pressure liquid coolant at a concentration of 0, 50, 100, 200 μg/mL extract was 24 hours a quartz tube order number attainments.

Blood glucose synthesis holds the balanced between the absorptive as glucose tissue glucose concentration. In the essential enzyme phosphoenolpyruvate caboxylase (PEPCK) between glucose in the synthesized on glucose-a 6 a-phosphatase (G6Pase) that compares the effect of expression of activation was.

As a result, glucose medium on PEPCK PFSE G6Pase activated by generating a concentration dependent protein expression has been confirmed by the decreased gradually. Positive controls the deflection in metformin AMPK activator, positive controls by providing reduced. Furthermore intracellular glucose were verify generating reduced in addition. While, according to an intracellular glycogen content of been increased. These results till quartz tube order number can be interprets to barge into a Corynebacterium ammoniagenes extract before billion (5 also).

In addition, quartz tube in order HepG2 activity by generating glucose extract medium (immunoblotting) p provided AMPK was analyzed through targeting blow adjuvants. Energy levels of signal molecules affecting intracellular AMP AMPK is activated if a fatty acid is increased to promote the oxidation of fat β - billion generation number to each other.

In addition, activated AMPK longitudinal muscle or adipose is sugar absorption control substrate. The metformin AMPK known as active factors between glucose in lowering blood sugar billion generation by number are known as disclosed. The sulfonamide-thiazolpyridine AMPK activation of the number 2 number development main target are disclosed.

Quartz tube in order extract promotes the phosphorylation of AMPK background such formate, activate the AMPK result of said intracellular party creation billion to the ball and ISDN number were cylindrical (6 also).

(5). Quartz tube order extract cytotoxic evaluation

In HEK-a 293T extract have cytotoxicity evaluation order quartz tube, the cells when the 96 - well plate 2 × 10 [...] in originating electrical fusion⁴ After culturing the cells/mL [...] 24 by time, sample concentration 24 hours and then subjected to treatment with a by further culturing, 10 μl, MTS [3 - (4, 5 a-dimethylthiazol-a 2 a-yl) - 5 - (3 provided carboxymethoxyphenyl) - 2 - (4 a-sulfophenyl) - 2H-a tetrazolium, inner salt)] solution injected into each well 37 °C him as material in 4 hours. ELISA plate reader in absorbance 490 nm were measured.

A high pressure liquid coolant in said number 2 number order extract attainments HEK-a 293T cells quartz tube 1, 10, 50, 100, 200, 400, or in a concentration of 1,000 μg/mL concentration range 10 provided 400 μg/mL and 1,000 μg/mL cell experiments have used processing even without toxicity as signal peptides (also 7).

2. Animal sprout quartz tube at the level represented by the formula

(1). Crude material represented by the formula in the model number 2 order quartz tube

(1) Effective and experimental design

This deficiency in the leptin receptor (leptin receptor) leptin resistance is generated and controlled appetite not obesity is induced, growth blood glucose increases in digging out a crude model number 2 (C57BL/KsJ-a db/db mouse) extract was constructed from a non-effect of lowering blood analyzing order quartz tube.

Quartz tube order accurately separate efficacy of lowering blood order quartz tube prior to experiment extract raw material, C57BL/KsJ-a db/db mouse weight loss, dietary and water intake, identifying when the Manchester carry blood glucose, analysis method efined 8 is also shown.

Effective between the [su which freezes the words issued from 5 week zero male C57BL/KsJ-a db/db mouse has been adapting this daytime which are the basic refer 1. After a period of adaptation according to the C57BL/KsJ-a db/db mouse diabetes supplying regulated [...][...] (AIN provided 93G) (ND), 0 normal diets. 1% Metformin drug controls (Metformin) supplement, 1 normal diets. 0% Pure army embryo into a quartz tube 10 by dividing the sprout quartz tube then supplement, dietary experiment number 5 was a daytime [...].

The automatic animal chamber environment (22 ± 1 °C), polyolefin (50 ± 5%) and 12 light period time interval hardships. Dietary and thus relieves a number so as to changing the water. In addition experiment 4 °C during the dietary storage rotates at the plastic, dietary years [...] rate his number. Experiments have shown to the dietary composition of table 6. The amount of water daily dietary intake and positive to negative number is subtracted value measures the performance of a residual amount of time was used.

During experimental week post-prandial blood glucose levels between weight and rate were measured. After the end of the breeding period, then 70% ether anesthesia before time then into ethanol to a nanoparticulate sacrificial mouse 12 after an overcurrent control his abdomen for disinfection. Taking a blood vein disrespectful treatment using a sterile syringe after an overcurrent abdomen after sterilization in conducting the experiments using the air tube.

The remaining blood was a stand-alone in PBS solution number by the King after blood organ, an organ in an aqueous, then his refrigerator weighing -70 °C. Long-term homopolymers crab age writing (Polytron, PT non-MR 3100, Switzedland) homogenizing the sample at night.

(2) Weight reduction and represented by the formula

As in of Figure 9 A, 1% extract significantly reduced weight when it has processed order quartz tube db/db mouse indications.

An influence on the blood pressure was analyzed post-prandial blood glucose directory once a week. As a result, as in of Figure 10 B, but not ending a larger difference between start and normal mouse glucose according to the experiments, the mean blood glucose 315 mg/dL diabetic controls (DM) ending, the positive controls (PC) 204 mg/dL, order processing group to diabetes significantly reduced compared to 159 mg/dL regulated quartz tube.

(3) Glucose tolerant extract influence on order quartz tube

For a load of 12 hours per mouse exposed to oral nanoparticulate (2. 5 G/kg) after orally administering, 120 minutes blood glucose concentration were measured.

10 Also result shown but, in the case of normal controls (ND) representing the highest blood glucose value 15 minute after administration, blood glucose after 30 minutes is reduced motor and the 120 minute normal levels. After 15 minutes in the case of diabetes controls (DM) have continuously increased temperature raced up to 60 minutes, 120 minutes initial blood 14 not recovering high blood homestretch.

While, positive controls and sulfates have elevated blood sugar level is reduced 60 min order quartz tube 15 processing group, initial blood 120 contracts result of sulfates have shown.

The order of administration is impaired when it is judged that the result of the quartz tube can be effective. Quit inspection to intravenous or oral pathway in blood sugar after the predetermined time measuring method for diagnosing insulin-secreting ability by indirectly identifying the contemporary president child diagnosis be utilized as substrate.

(4) Insulin synthesis of beta cells influence on pancreatic tissue extract order quartz tube

The degradation of beta cells use of insulin resistance number 2 typical pathological features. Of beta cells function and morphological evaluation hematoxylin & eosin (H&E) insulin antibody immune histological dyeing can be a metal thin film.

Normal mouse voice controls (ND), diabetic controls (DM), positive controls (PC), quartz tube order processing group (PF) pancreatic of beta cells morphological properties taken insulin expression tendency for flaws.

Long-term beta cells upon exposure to high concentration glucose glucose toxicity side effects such as insulin secretion capacity falls and representing opening degree, non-reversible beta pharmaceutically acceptable substances known insulin dielectric transcription factor reference book.

H E&on immune dyeing camera into diabetic controls of morphological is a production has been damage of beta cells, insulin immune response to confirm it capable of exhibiting reduced number of beta cells. Positive controls and administration group-number 2 [...] quartz tube to an immune response against the insulin beta cells order quartz tube charge involved in beta cell protecting or silicide by ion implantation crude be inferred substrate (11 also).

(2). Crude drug extract number 2 C57BL/KsJ-a db/db mouse animal diabetic effect order quartz tube

(1) Experimental design and animal diabetic efficacy measurement method

Effective between the [su which freezes the words issued from 5 week zero male C57BL/KsJ-a db/db mice been adapting this daytime which are the basic refer 1.

After a period of adaptation according to a diabetes (ND) C57BL/KsJ-a db/db mice [...] regulated, drug controls (Rosiglitazone, 3 mg/kg), oral [...] order quartz tube to extract order quartz tube lightly extract (50 mg/kg), moderately (250 mg/kg), high concentration (500 mg/kg) was administered orally daily divided into saline for 1 to 3. Regulated curcumae controls are only normal saline was administered. 6 Then each group by dividing the embryo, daytime 4 was administered.

The automatic animal chamber environment (22 ± 1 °C), polyolefin (50 ± 5%) and 12 light period time interval hardships. Dietary and thus relieves a number so as to changing the water. Rate week dietary intake and negative rate were measured.

The week by an influence on post-prandial blood glucose rate post-prandial blood sugar, a nanoparticulate measured 12 hours then, after tail vein in body [hyel (Roche Diagnostics GmbH, Germary) post-prandial blood glucose concentrations were measured using a simple blood glucose orgin.

After 12 hours and measuring glucose intolerance (Oral glucose tolerance test, OGTT) is administered 3 main glucose solution to a nanoparticulate (2. 0 G/kg BW) after oral administration, blood glucose change through tail vein body [hyel observed. 15, 30, 60, 90, 120 Minutes after administration in lowering blood glucose changes in concentration were measured.

(2) Extract diabetic efficacy measurement result order quartz tube

Order quartz tube After post-prandial blood glucose change

Experiment 12 also a result of the determination shown in last 4 post-prandial efined parking to blood glucose. Diabetic controls are regulated 258 ± 53 mg/dL normal as of 67 ± 6. Compared to 5 mg/dL significantly (P < 0. 05) Characterized in that it has increased fasting blood glucose as signal, positive controls are 163 ± 44 mg/dL, quartz tube order processing group processing multiple respectively, 211 ± 9. 5 Mg/dL, 156 ± 35 mg/dL, 151 ± 21 mg/dL shown as positive controls similar aspect.

Dietary intake, Water intake, an influence on weight change

Experimental dietary intake during week by dietary intake positive regulated degree of deterioration of other as statistical significance in that it has a sliding is performed, significant differences can be order quartz tube has been identified cannot even administration group (13 also).

During experimental week by negative intake positive controls higher than negative amount was visible, the highest processing concentrations low negative amount processing group 500 mg/kg extract order quartz tube and viscoelasticity (14 also).

Weight change week by analyzing result, population group number [...] normal weight gain was visible all persistent, diabetes (also 15) been weight gain is greater than rosiglitazone troops army army regulated positive contrast processing.

This class of drug is PPAR γ thiazolidindione rosiglitazone (TZD) administration of the insulin resistance improving through stimulating insulin reduction in blood glucose level increase effective but, fat differentiation by activating an obesity, edema reported that corrosion and failure to the median, differentiation of the fat cells associated with the palpated by the low fat or bodily fluid according to the edema resulting from drug wirelessly reports in the nanometer range. While, statistical significance is reused, diabetes compared to order processing group to a lack weight gain rate regulated quartz tube and viscoelasticity.

Glucose intolerance(Oral glucose tolerance test, OGTT) Measurement

Oral glucose intolerance test (OGTT) glucose in blood according to measured blood glucose change reference time before oral administration, 30 the highest blood glucose administration of sulfates bix, 120 minutes trend of reduced. Administration group as compared to an increase in blood glucose regulated low tendency diabetes quartz tube order extract precursor, 120 minutes have shown less blood (also 16).

In the embodiment 1 to 4><(number of extract including sausage order quartz tube bath)

(Unit:% by weight) for the repairing material according to table 4, a high pressure liquid coolant in said number 2 number attainments sausage was functional extract number order quartz tube high pressure liquid coolant.

, the number of degree and a process but pore sol of Figure 17 number sausage, beef fat (lampwick peripheral) therein and a first money A hinted with flesh-gate and dried. Said excised tissue meat particles size chopping and 6 - 8 mm, with respect to the maintaining of meat after chopping [...] within 1 provided 2 °C.

Silence cutter [...] beef 85. 0%, Money A fat 5. 0%, Salt 0. 2%, Sugar 0. 2%, Composite phosphate 0. 1%, Beef taste seasoning 1. 0%, Starch 3. 0%, Strip two protein 1. 0%, Pepper 0. 6%, Green onion [sul powder 1. 0%, [Pas other mix 1. 4%, Quartz tube order powder 0. 5%, Quartz tube order extract powder 1. 0% Oil which has been added.

Then, 20% degree so as to inhibit ice preparation prepared meat contrast, 1/3 have divided by input, in order to create a cutting is 10 minutes, 10 °C hereinafter been combination meat width it comes to maintain.

12 Formulation ofan if air enters the tenderness in combination tenderness 2 provided 3 °C aging time vacuum stuffer (stuffer) interposed in money market is placed nozzle after filling, then every period both ends securely, rod 30 minutes drying surface to rod 60 °C to 50 °C smoke smoke smoke chip with respect to the 40 minutes smoke.

30 Minutes smoke rod 75 °C heated steam, so that center temperature 25 °C then the water shower, 3 °C 2 in time to cool-drying, sausage extract powder including his number order quartz tube high pressure liquid coolant.

But, in the embodiment 3 in table 4 and in the embodiment 4 is inserted to said smoke of a process, quartz tube order extract powder including high pressure liquid coolant sausage his number.

Figure 3 in the embodiment 1 revealing the sausage prepared by the number by the seam number article are disclosed.

Example 3><experiments (functional test)

Said in the embodiment 1 to 4 including a quartz tube order number to a test object in high pressure liquid coolant extract sausage flavor, taste, color, overall preference for panel (20 to, to 30, 40 to each male and female 5 name, total 30 name selection) to embodiment allows functional evaluation, 5 measure point method (very a is: 5, a is: 4, usually: 3, the adsorption fixing plate: 2, defect: 1) preference to a visit from the police.

5 Result shown but to table, the reference table 5, the door frame in the embodiment 3 can be best overall gastrodia root.

In the embodiment 5><number bath (1) pharmaceutical compositions

A quartz tube order number in said number 2 high pressure liquid coolant attainments extract 10 mg, 100 mg corn starch, lactose-100 mg 2 mg magnesium salt of stearic acid and mixed with, a number of conventional manufacturing method including a lubricant homogeneously and switching order number was positive number according to quartz tube extract high pressure liquid coolant.

In the embodiment 6><number bath (2) pharmaceutical compositions

A quartz tube order number in said number 2 high pressure liquid coolant 10 mg extract attainments, crystalline cellulose 3 mg,

Lactose 14. 8 Mg and magnesium stearate 0. 2 Mg mixed with, a high pressure liquid coolant filled gelatin capsule according conventional capsule number manufacturing method produced number his number.

In the embodiment 7><number bath (3) pharmaceutical compositions

According to the manufacturing method of conventional scanning number per 1 ampoule (2 ml) in a quartz tube order number attainments said number 2 high pressure liquid coolant extract 10 mg, 180 mg of mannitol, sterile distilled water for injection and 2974 mg Na₂ HPO₄ , 12H₂ The composition of the scanning number O 26 mg a number was high pressure liquid coolant.

As above-mentioned, preferred number attainments of the present invention, experiments and through a browser but could be in the embodiment, if a corresponding splicing one skilled art to the present invention within a range that the idea of the present invention fee so as to range from a heavy and area from various corrected may be understand and changing it will rain.