SENSOR SYSTEM FOR DETECTING ORGANOPHOSPHORUS PESTICIDE RESIDUES BY INDUCING COAGULATION OF GOLD NANOPARTICLES

Otherwise disassociated is not defined, the present specification all used in the technical and scientific terms are in the present invention is in the field of the conventional by operational information includes a equivalent to those that would have been understood. have the meanings. Generally, the present specification the designated used in the is well known in the art the condensate is might be used.

In the present invention, an organophosphorous of lignin to pesticide solution or imidazole solution and gold nanoparticles by the addition of GFP solution absorbing spectrum measuring changes in an optical dielectric polymeric material and film substrate and to an experiment using a computer to is performed for all the. As a result, an organophosphorous and desirably imidazole or GFP agglomeration of the nano sized particles has a value of a range of gold, the strongest in absorbance (peak) 600-700nm representing a new spectrum is generated confirm that the user has been, the resulting a visible change to an organophosphorous agricultural chemicals easy and rapid that the identifying improving can be detected.

Therefore, the present invention refers to in one aspect, pesticide (a) mixed solution of gold nanoparticles samples and contamination of the imidazoles solution, histidine solution, pyrazole solution, histamine solution and GFP solution selected from the group consisting of adding-electrolytic gold 1 species least of a solution and inducing an agglomeration of the nano sized particles; and (b) said gold according to agglomeration of the nano sized particles involves measuring the absorbance in 600-700nm including an organophosphorous of agricultural chemicals relates to detecting method.

Pesticide contamination sample of the present invention, comparing the pollution due to pesticide residue, such as from a the crops. the samples obtained.

Gold tetrachloride particles gold nanoparticles in one in the embodiment of the present invention (III) acid citrate sheath can be synthesis of reduction. Gold nanoparticles in liquid phase and the method of producing particles without using a template (template) in temperature processing conditions using a template and method for reducing a metal salt function at a normal temperature by synthesizing is connected to the semiconductor layer. method.

Template without using metal salt in a solvent and method senses a rotation velocity of the disk for the stirring the disk or more boiling conditions (stirring) in citrate (citrate) a reducing agent is added to obtain the metal nano particle collector. Another method formed on gold nanoparticles in thermodynamic unstable state or surface stabilizer to overcome the. a phenyl-based alkoxysilane compound in template. Optionally mesoporous alumina and silica, such as carbon nanotube (CNT) (surfactant) hard template (hard template) with such as soft template use an (soft template). Using template method of producing particles using the seed (seed) and a growth solution (growth solution) a seed based (seed-mediated) crystal growth method such as method (polyol process) and polyol.

Gold nanoparticles bio sensing, in particular in the fields of simplifying a nano material that has. Gold nanoparticles particles generally compared to an organic dye is 10³ -10⁵light absorption coefficient greater (extinction coefficient) has a unique UV/vis. spectrum of. The gold nanoparticles under visible light and has color, specific liquid sample to a specific binding employed gold nanoparticles in which causing agglomeration of self, the gold nanoparticles which to bring about a change dielectric constant powder, surface plasmon topical eventually is placed in conditions absorbent (LSP) under visible light is gold nanoparticles solution is whose own color changes. I.e. nanoparticles closer face up, the distance between the absorption wavelength light while red transition (red-shift) red of the original is generated in signal according to progress of the increasingly blue is varying.

In the present invention an organophosphorous pesticide, imidazole, gold nanoparticles the reaction specific between particles revealed when a solution by using the variation own color special analysing equipment object even eye without the liquid sample to asked to develop a colorimetric sensor (colorimetric sensor).

519 nm gold nanoparticles in spectrum measurement UV/vis particles upon the strongest absorbance (peak). to have. However gold nano-particle of imidazole as a result of response to a pesticide aggregated, , 600 to 700 nm, preferably at a wavelength of 670 nm are spectrally contained to having the strongest absorbance (peak) optical dielectric polymeric material and film substrate and resistance in an as measured by the (UV/vis spectrophotometer) lies.

In the present invention the green fluorescent protein GFP used, preferably that is capable of using optical of the present invention embodiment as shown in the example EGFP (Enhanced green fluorescent protein). The EGFP in kind of fluorescent protein GFP, GFP 1-2 in one another is amino acids, along with the toughened substituted. green fluorescent protein. General GFP is stopped and compressed air stronger than having properties fluoresce.

In one in the embodiment of the present invention, in order to obtain recombinant EGFP EGFP gene from e.coli, for transforming DNA, that induces the expression EGFP and purifying, the third to eo. An organophosphorous pesticides and gold nanoparticles must a fluorescent protein reacts with GFP or are not limited only EGFP, RFP (red fluorescent protein), CFP (cyan fluorescent protein), YFP (yellow fluorescent protein), BFP (blue fluorescent protein), EGFP (enhanced green fluorescent protein), ECFP (enhanced cyan fluorescent protein), EYFP (enhanced yellow fluorescent protein), ERFP (enhanced red fluorescent protein), EBFP (enhanced blue fluorescent protein) or to a fluorescent protein various including a can be replaceable.

The present invention refers to in another aspect, (a) pesticide mixed solution of gold nanoparticles samples and contamination of the imidazoles solution, histidine solution, pyrazole solution, histamine solution and GFP solution selected from the group consisting of adding-electrolytic gold 1 species least of a solution and inducing an agglomeration of the nano sized particles; 600-700nm absorbance according to agglomeration of the nano sized particles gold (b) said surveying step; and (c) said measured based on absorbance step of quantifying concentration of agricultural chemicals including an organophosphorous of agricultural chemicals relates to quantitative method.

In one in the embodiment of the present invention, an organophosphorous of lignin to pesticide EFGP solution or imidazole solution and gold nanoparticles when mixing solution, concentration of pesticide included because of the difference (absorbance) absorbance according to a highly a few weeks, gas is fed into a tube the difference at a wavelength 670 nm reliably a was capable of confirming the. Therefore, also 4 and 13 as demonstrated, the added value to the subscriber's absorbance 670 nm in wavelength concentration of agricultural chemicals according to standard curve representing a few weeks, 3 sigma method (average above and below measures of video 3 times width for holding a method determining a limit) each instrumental detection limits using been is calculated as a 17ppb or 27.9ppb, said second (plateau) plateau at concentrations near 3 PPM an organophosphorous pesticide detectable sensor to a range was capable of confirming the in 0.01-3ppm.

In another of the present invention in the embodiment, the present invention and quantitative pesticide an organophosphorous using organic phosphorus pesticide quantitative HPLC (High-performance liquid chromatography) when compared to result, GFP or imidazole particles and gold nanoparticles using accurately and by HPLC method is quantitative pesticide of the present invention, a rapid and efficient than HPLC method method for preparing steel HPLC as metals and may replace light could confirm it.

By using absorbance of the specific A = εLc the when computing a density use of formula. Wherein the absorbance (absorbance) is A A =-log (I/I₀) is defined by. I₀ of a material before passing it through a material I is light intensity passing through a is light intensity. The L, which pass through the (cuvette) quantity of the supplied sample distance is unique of the material to a ε light absorption coefficient (molar absorbity) is calcium, the c is (molar concentration) molar concentration of the sample. When the employee ε and L of the absorption edge of the solution by measuring the (A) may be as to obtain the concentration solute.

In the present invention, sample contamination pesticide methanol, ethanol, or an aqueous solution of the hypertension dilution may additionally comprise.

In one in the embodiment of the present invention, an organophosphorous agricultural chemicals EFGP solution or imidazole particles and gold nanoparticles before reacting the solution and, aforementioned synchronous signal which melts methanol was 10%. Pesticide an organophosphorous from the sample is an agricultural-chemical contamination for extracting.. Methanol instead a solvent having properties similar either using a ethanol, aqueous solution of methanol or ethanol ID.

In the present invention, the diameter of the gold nanoparticles said 10-50nm can be characterized in that the. When 10-50nm particle diameter of gold nanoparticles that it represents a red, an organophosphorous and desirably imidazole due to agglutinate with gold when embolic is developed: in a reflection display area R best.

In the present invention, a diaryl pesticide an organophosphorous said (diazinon) triazinones, editing pen pos (edifenphos) or with it cuts pos (iprobenfos) can be. As an organophosphorus compound alkyl or formula1, including a phosphorous atom (P) a compound that is joined to the, dichloro boss (Dichlorovos), the para mote it comes (parathion), (malathion) it dries and the mote it comes, patrol reel nose (Tebuconazole), (acetamiprid) [...], EPN, oxide layer using wet [...] (fenitrothion), or even as an organophosphorus compound such as (fenthion) the pen mote it comes detecting method of the present invention can be applied is method and quantitative.

In the present invention, said gold nanoparticles 8-12nM and the concentration of solution, the concentration of imidazole solution 0.1-0.4mM and the shaft transfers the, an organophosphorous said 0.01-3ppm concentration detecting of agricultural chemicals which may be, the pH optimum position of the pesticide an organophosphorous said 7.4-8.9, more preferably 7.4-8.4 can be characterized in that the.

In of the present invention in the embodiment, gold nanoparticles 10 nm on concentration of solution, imidazole solution concentration of 1:1 volume ratio of 0.3 mm, the one by mixing an organophosphorous when has come about, best reactions with pesticide. Furthermore, pH of the mixed solution of 8.4 is an organophosphorous of agricultural chemicals according to whether one when a highly a absorbance difference, an organophosphorous of agricultural chemicals is the most suitable for detecting pH that could see he.

Gold nanoparticles in another aspect the present invention refers to solution; and imidazole solution, histidine solution, pyrazole solution, histamine solution and GFP solution selected from the group consisting of least of a solution and species 1 ; for including an organophosphorous pesticide relates to kit for detecting.

In the present invention, the concentration of solution gold nanoparticles said 8-12nM and, imidazole solution, histidine solution, pyrazole solution, histamine solution and GFP solution selected from the group consisting of the concentration of species 1 0.1-0.4mM least of a solution and can be.

[In the embodiment]

Hereinafter, embodiment to the present invention. as further described further. These embodiment only relate for examples of the present invention, and/or at least two different embodiment of the present invention range interpreted to be limited in the art does not to be will nontrivial twiddle factors and person with skill in the art. Thus substantial of the present invention by issuing an ranges are defined by claim and their equivalent will the pixels include.

In the embodiment using imidazoles to particles and gold nanoparticles

Preparation of equipment sample and

Gold nanoparticles used in the manufacture of the (trisodium citrate dehydrate) hydrate citric BIO BASIC (CANADA INC.) 2 purchased from the n bit parallel data inputted three natrium, gold tetrachloride (III) acid (Gold (III) chloride hydrate, HAuCl₄) for purchasing a from the Sigma-Aldrich (USA). Gold nanoparticles (III) acid citrate sheath gold tetrachloride particles synthesized reduction corresponding advertisement based on the shown list, 13 nm in diameter, the third to eo at below gold nano particle.

An organophosphorous and insecticidal diaryl (diazinon) triazinones, editing pen pos (edifenphos), pipes buffering saltwith it cuts pos (iprobenfos) and phosphoric acid (phosphate buffered saline, tablet) for purchasing a from the Sigma-Aldrich (USA).

Merck chemicals in the methanol, imidazole the BIO BASIC (CANADA INC.) purchased from the n bit parallel data inputted, Milli-Q grade water (18.2 M Ω cm, Millipore) been used at all.

Is affectable is measured with Synergy H1 Hybrid Reader purchased from a Biotek (Korea).

In the embodiment 1: an organophosphorous of lignin to pesticide gold nanoparticles particles and/or imidazole the addition of absorbing spectrum change measurement according to

First, an organophosphorous and insecticidal diaryl respect to (diazinon) triazinones, pesticide 10 micro l melt to a 10% methanol 10 ml, 1000 PPM concentration was fitted. 10% methanol to the dilution to be, at a concentration of 1 PPM dilution the samples have been prepared.

An organophosphorous of lignin to pesticide gold nanoparticles a writing time can increase slant detector detects the increase slant, and imidazolidinones light absorption spectrum change takes place is (movement of peak), which change this element a supervis ion portion confirms a paradigm was developed.

1-1. Triazinones diaryl (diazinon) and gold nanoparticles when the reaction solution

First, an organophosphorous and insecticidal diaryl (diazinon) triazinones 1 PPM dilution and a solution of concentration 10 nm gold nanoparticles solution function at a normal temperature by blended at a volumetric ratio of 2:1 reacted.

As a result, also decodes a such as 2, absorbing unique gold nanoparticles exhibit resistance changes in spectrum peaks at about 520 nm (peak), an organophosphorous and insecticidal diaryl even when reacting refutation gold nano particle peak gold nanoparticles out 520 nm identically position (peak). Therefore, diaryl gold nanoparticles of absorption spectrum at alone triazinones directly which exerts no influence was capable of confirming the.

1-2. Imidazole solution and when the reaction solution gold nanoparticles

An organophosphorous pesticide except that the, 0.3 mm imidazole solution and 10 nm gold nanoparticles an amorphous 1:1 blended at a volumetric ratio of the absorbance measured by, special of peaks in Figure 2 such as a mobile is found an, imidazole only gold nanoparticles alone can impart a spectrum of absorption that not can be identifying.

1-3. Triazinones diaryl (diazinon), imidazole solution, and gold nanoparticles solution the reaction together when the

An organophosphorous and insecticidal diaryl 1 PPM a dilution solution concentration (diazinon) triazinones, 0.3 mm imidazole solution, 10 nm gold nanoparticles solution function at a normal temperature by blended at a volumetric ratio of 2:1:1 reacting a metal salt of absorbing for measuring the spectrum when the, in Figure 2 such as a been is generated peak new 670 nm, pesticide imidazolyl therefrom in response to components that are useful for actively inducing agglomeration of the nano sized particles gold, gold nanoparticles of absorption spectrum has influence upon a representative area suitable for the data is an organophosphorous of agricultural chemicals can be confirm that the user.

Editing pen pos (edifenphos), pipes (iprobenfos) pos with it cuts, it dries and the mote it comes (malathion), (Tebuconazole) patrol reel nose, [...] (acetamiprid), oxide layer using wet [...] (fenitrothion), and support members have smaller the pen mote it comes (fenthion) the n bit parallel data inputted and conducts an experiment same, results of the same type can be obtained.

In the embodiment 2: measuring absorbance by concentration of agricultural chemicals an organophosphorous each

Triazinones diaryl (diazinon), editing pen pos (edifenphos), pipes (iprobenfos) pos with it cuts, it dries and the mote it comes (malathion), (Tebuconazole) patrol reel nose, [...] (acetamiprid), oxide layer using wet [...] (fenitrothion), and each an organophosphorous of lignin to pesticide the pen mote it comes (fenthion), such as in the embodiment 1 in concentrations of various range of 0.01-10.0ppm method wherein an agrochemical technical product having samples have been prepared. Furthermore, each concentration of an organophosphorous pesticide sample, of 10 nm gold nanoparticles solution, 0.3 mm imidazole solution the blended at a volumetric ratio of 2:1:1, optical dielectric polymeric material and film substrate and absorbing to 400-700nm change of spectrum are measured at range (also 3), the standard curve is obtained (also 4).

As a result, also 3 as demonstrated, absorbing spectrum of a peak in position but the signals to corresponding to concentration of agricultural chemicals to 670 nm, because of the difference (absorbance) absorbance according to the concentration of agricultural chemicals a highly a few weeks, gas is fed into a tube the difference at a wavelength 670 nm reliably shown. Accordance with 4 such as a, absorbance 670 nm in wavelength according to concentration of agricultural chemicals the added value to the subscriber's standard curve representing a few weeks, 3 sigma method (average above and below measures of video 3 times width for holding a method determining a limit) 27.9 PPM a instrumental detection limits using been calculated, said second (plateau) plateau at concentrations near 3 PPM an organophosphorous pesticide detectable sensor to a range was capable of confirming the in 0.01-3ppm.

Compared e.g.: the control group agrochemical compound non pesticide an organophosphorous using confirming specificity detecting

Gold nanoparticles particles and imidazoles to including only pesticide an organophosphorous a sensor system detecting pesticide specific to supervis ion portion confirms a reaction, an organophosphorous of agricultural chemicals each of the includes a formula on a non functional (benzene) benzene is agrochemical compound, phenol (phenol), toluene (toluene), xylene (xylene), die trichlorobenzene (dichlorobenzene), phosphoric acid (phosphoric acid) so that each 10% methanol 1 PPM concentration dilution and a solution, solution gold nanoparticles of 10 nm, a ratio, by volume, of 2:1:1 0.3 mm imidazole solution mixed.

670 nm absorbance in the analyzed result, also 5 as demonstrated, non agrochemical compound is benzene, phenol, toluene, xylene, trichlorobenzene die, phosphoric acid or the like suffers significantly low absorbance for 0.33 hereinafter, an organophosphorous and insecticidal diaryl only of the absorption edge of the (Diazinon) triazinones 0.41 reduce the power consumption by controlling properly shown, an organophosphorous and insecticidal specific reactions to the urgency of the task and the was capable of confirming the.

In the embodiment 3: an organophosphorous pesticide, gold nanoparticles, and imidazole mixture zeta potential change

Gold nanoparticles solution an organophosphorous and desirably imidazole solution when sequentially adding at least one (same as in the embodiment 1-3 the lamp and ventilation concentration), each addition according to gold nanoparticles of hydrophobic additive is soluble in zeta potential is measured with particle. And -35mV have positive zeta potential of gold nanoparticles, an organophosphorous agricultural chemicals -36mV increase slant detector detects the increase slant is there was hardly any changes. However on each sample of the imidazoles -12mV both increase slant detector detects the increase slant to second, gold imidazolyl a large agglomeration of the nano sized particles for impacting subsequent to the in the embodiment 1-3 can be set (also 6).

In the embodiment 4: change particle size of gold nanoparticles

Gold nanoparticles solution an organophosphorous and desirably imidazole solution when sequentially adding at least one (same as in the embodiment 1-3 the lamp and ventilation concentration), each addition according to size of gold nanoparticles is measured with particle zeta potential. And 13 nm particle diameter of gold nanoparticles, an organophosphorous agricultural chemicals to increase slant detector detects the increase slant 13 nm cannot change in amplitude. However on respectively, if increase slant detector detects the increase slant of the imidazoles each sample 202 nm, to second 235 nm, gold imidazolyl a large agglomeration of the nano sized particles for impacting subsequent to the in the embodiment 1-3 can be set (also 7).

In the embodiment 5: an organophosphorous pesticide, and imidazole mixture change absorbance pH according to gold nanoparticles

Gold nano-particle an organophosphorous and desirably imidazole react with pH of solution when to find-sensitive data according to 670 nm human power by operating all systems by pH change in absorbance change (same as in the embodiment 1-3 the lamp and ventilation concentration).

A pH 3.4 10.4 placed on an inner space in which the analyzed result absorbance, in Figure 8 such as a, high pH conditions 670 nm of 1 PPM and 0 PPM pesticide an organophosphorous a farther module and to difference absorbance in the capacitor is formed by connecting a was capable of confirming the. The highest difference using the pH 8.4.

In the embodiment 6: deionized water(deionizedwater) and water (tap water) and quantitative pesticide applied to organic phosphorus pesticide quantitative HPLC comparison of result

Gold nano particle an organophosphorous pesticides and imidazole and the measures the absorbance reacting a metal salt of deionized water with tap water, to the quantitative results in concentration of agricultural chemicals, generally employs an agricultural-chemical detecting method obtained by a HPLC (High-performance liquid chromatography) analysis as compared to quantitative result, accuracy of quantitative pesticide an organophosphorous of the present invention method paradigm was developed a be verified using BIAcore (gold nanoparticles, imidazole solution same as in the embodiment 1-3 concentration of the lamp and ventilation).

Deionized water (deionized water) and water (tap water) and quantitative pesticide applied to organic phosphorus pesticide quantitative HPLC and compared result.

* 3 burn on a result of the average values; SD: standard deviation

As a result, as seen from in table 1, an organophosphorous of agricultural chemicals 0.080 PPM concentration, after dilution in 0.300 PPM and 0.170 PPM, of the present invention method and on which the second photoresist layer is quantitative analysis using HPLC results, without the difference has been similar. Quantitative method of the present invention when it is used for average recovery (recovery) 100.1% in deionized water, tap water, to the. present method for preparing steel HPLC in 90.5% of 99.4% as little time for the error rate. Thereby accurately and by HPLC method is quantitative pesticide of the present invention, a rapid and efficient than HPLC method method for preparing steel HPLC as metals and may replace light could confirm it.

In the embodiment using GFP particles and gold nanoparticles

Preparation of equipment sample and

Gold nanoparticles used in the manufacture of the (Trisodium citrate dehydrate) dihydrate three natrium citric BIO BASIC (CANADA INC.) purchased from the n bit parallel data inputted, gold tetrachloride (III) acid (Gold (III) chloride hydrate, HAuCl₄) for purchasing a from the Sigma-Aldrich (USA). Gold nanoparticles (?) acid citrate sheath gold tetrachloride particles synthesized reduction corresponding advertisement based on the shown list, 13 nm in diameter, the third to eo at below gold nano particle.

An organophosphorous and insecticidal diaryl (diazinon) triazinones, editing pen pos (edifenphos), pipes for purchasing a from the (iprobenfos) and phosphate buffered saline (tablet) pos with it cuts Sigma-Aldrich (USA).

Merck chemicals for purchase in the n bit parallel data inputted the methanol, Milli-Q grade water (18.2M Ω cm, Millipore) been used at all.

Recombinant has EGFP, for transforming DNA, that induces the expression from e.coli and purifying, the third to eo.

And the fluoresced and is affectable is measured with Synergy H1 Hybrid Reader purchased from a Biotek (Korea).

In the embodiment 7:an organophosphorous and insecticidal EGFP to activation of impact and with the exploitation of confirming

First, an organophosphorous and insecticidal diaryl respect to (diazinon) triazinones, pesticide 10 micro l melt to a 10% methanol 10 ml, 1000 PPM concentration was fitted. 10% methanol to the dilution to be, 10ppb, 100ppb, 1 PPM, 10 PPM, or have inadequate concentrations of these various been produced with at each sample dilution.

An organophosphorous of lignin to pesticide EGFP by intensity of fluorescence of fluorescent to supervis ion portion confirms a would otherwise be operative, represents a respectively different are fabricated on wherein an agrochemical technical product having concentration added EGFP sample after 3 min at room temperature, the control group on 10% methanol, each at concentrations EGFP so as to result in excitation intensity of fluorescence of fluorescent protein (excitation) 480 nm, it was determined that at a wavelength emitting (emission) 510 nm (also 10).

As a result, also 10 as demonstrated, according to concentration of agricultural chemicals by releasing EGFP other intensity of fluorescence can be confirm that the user appears. The intensity of fluorescent increases increased concentration of pesticide appears purchases the passenger strong, concentration of 10 PPM the intensity of fluorescent coupled by-pyrithione is provided.

Editing pen pos (edifenphos), and with it cuts pos (iprobenfos) the n bit parallel data inputted performing experiment such as support members have smaller, the same result can be obtained.

In the embodiment 8: an organophosphorous of lignin to pesticide EGFPparticles and/or gold nanoparticles absorbing spectrum change measurement according to are added thereto

An organophosphorous of lignin to pesticide EGFP particles and gold nanoparticles rapid time for displaying a menu (movement of peak) light absorption spectrum change takes place is, this element which change find a paradigm was developed.

8-1. Triazinones diaryl (diazinon) and gold nanoparticles when the reaction solution

First, an organophosphorous and insecticidal diaryl (diazinon) triazinones 1 PPM dilution and a solution of concentration 10 nm gold nanoparticles solution function at a normal temperature by blended at a volumetric ratio of 2:1 reacted.

As a result, in Figure 11 such as a, absorbing unique gold nanoparticles about 520 nm peaks at the planar sheet to exhibit a spectrum, an organophosphorous and insecticidal diaryl even when reacting refutation gold nano particle of gold nanoparticles out 520 nm identically peak location. Therefore, diaryl gold nanoparticles of absorption spectrum at alone triazinones directly which exerts no influence was capable of confirming the.

8-2. Gold nanoparticles solution and EGFP when the reaction solution

An organophosphorous pesticide except that the, 5 micro g/mL EGFP solution and 10 nm gold nanoparticles an amorphous 1:1 blended at a volumetric ratio of the absorbance measured by, special of peaks in Figure 11 such as a mobile is found an, gold nanoparticles alone only EGFP can impart a spectrum of absorption that not can be identifying.

8-3. Diaryl (diazinon)triazinones, EGFPsolution, and gold nanoparticles solution the reaction together when the

An organophosphorous and insecticidal diaryl 1 PPM a dilution solution concentration (diazinon) triazinones, 5 μg/mL EGFP solution, 10 nm gold nanoparticles solution function at a normal temperature by blended at a volumetric ratio of 2:1:1 reacting a metal salt of absorbing for measuring the spectrum when the, in Figure 11 such as a been is generated peak new 670 nm, pesticide is EGFP therefrom in response to components that are useful for actively inducing agglomeration of the nano sized particles gold, gold nanoparticles of absorption spectrum has influence upon a representative area suitable for the data is an organophosphorous of agricultural chemicals can be confirm that the user.

Editing pen pos (edifenphos), and with it cuts pos (iprobenfos) the n bit parallel data inputted performing experiment such as support members have smaller, the same result can be obtained (also 12).

In the embodiment 9: measuring absorbance by concentration of agricultural chemicals an organophosphorous each

Triazinones diaryl (diazinon), editing pen pos (edifenphos), pipes of lignin to pesticide an organophosphorous each (iprobenfos) pos with it cuts, such as in the embodiment 7 0.01-10.0ppm in method wherein an agrochemical technical product having concentrations of various range of have been prepared samples. Furthermore, such as in the embodiment 8, each concentration of an organophosphorous pesticide sample, solution gold nanoparticles of 10 nm, 5 micro g/mL EGFP of the blended at a volumetric ratio of 2:1:1 solution, optical dielectric polymeric material and film substrate and absorbing to 400-700nm change of spectrum are measured at range (also 12), the standard curve is obtained (also 13).

As a result, can be collected and in Figure 12,670 nm absorbing spectrum of a peak in position but the signals to corresponding to concentration of agricultural chemicals to, because of the difference (absorbance) absorbance according to the concentration of agricultural chemicals a highly a few weeks, gas is fed into a tube the difference at a wavelength 670 nm reliably shown. Accordance with 13 such as a, absorbance 670 nm in wavelength according to concentration of agricultural chemicals the added value to the subscriber's standard curve representing a few weeks, 3 sigma method (average above and below measures of video 3 times width for holding a method determining a limit) using is calculated as a 17ppb been a instrumental detection limits, said second (plateau) plateau at concentrations near 3 PPM an organophosphorous pesticide detectable sensor to a range was capable of confirming the in 0.01-3ppm.

Compared e.g.: the control group agrochemical compound non pesticide an organophosphorous using confirming specificity detecting

EGFP particles and gold nanoparticles including a pesticide an organophosphorous system colorimetric sensor detecting pesticide specific only to supervis ion portion confirms a reaction, an organophosphorous of agricultural chemicals each of the includes a formula on a non functional (benzene) benzene is agrochemical compound, phenol (phenol), toluene (toluene), xylene (xylene), die trichlorobenzene (dichlorobenzene), phosphoric acid (phosphoric acid) so that each 10% methanol 1 PPM concentration dilution and a solution, solution gold nanoparticles of 10 nm, 5 micro g/mL EGFP of a ratio, by volume, of 2:1:1 solution mixed.

670 nm absorbance in the analyzed result, also, such as 14, non agrochemical compound is benzene, phenol, toluene, xylene, trichlorobenzene die, phosphoric acid or the like suffers significantly low absorbance for 0.35 hereinafter, an organophosphorous and insecticidal diaryl (Diazinon) triazinones, editing pen pos (Edifenphos), 0.41 only of the absorption edge of the (Iprobenfos) pos with it cuts pipes reduce the power consumption by controlling properly shown, an organophosphorous pesticide specific reactions to the urgency of the task and the was capable of confirming the.

In the embodiment 10: an organophosphorous pesticide, gold nanoparticles, absorbance change according to time mixture EGFP and

extinction largest change in 670 nm, an organophosphorous of agricultural chemicals according to time having different concentrations and absorbance value by measuring a most and a reactor suitable for paradigm was developed a user input. An organophosphorous pesticide (diaryl triazinones, each 0,0.01, 0.1, 1.0 PPM concentration), gold nanoparticles, since the added to an event that both a EGFP 1 it was determined that value absorbance minutes.

As a result, initially like and a in Figure 15 the rate of change is cursor extinction abruptly reaction rates the n bit parallel data inputted which causes the disturbance of blood circulation, is and react accordingly most when display data signal to absorbance value larger difference between its concentration of agricultural chemicals according to it is shown that has been confirmed. A space of time very short reaction time method for measuring selected in that representative area suitable for the data is capable of confirming the which, long of time continue reaction occurs may confirm it is.

In the embodiment 11: change particle size of gold nanoparticles

Gold nanoparticles solution an organophosphorous and desirably EGFP when sequentially adding at least one solution (same as in the embodiment 8-3 the lamp and ventilation concentration), each addition according to size of gold nanoparticles it was determined that analyzer nanoparticles. And 11.7 nm particle diameter of gold nanoparticles, an organophosphorous agricultural chemicals increase slant detector detects the increase slant but less variation to 12 nm, EGFP time for displaying a menu to second 520.8 nm, gold EGFP a large agglomeration of the nano sized particles for impacting subsequent to the in the embodiment 8-3 can be once again able to identifying (also 16).

In the embodiment 12: an organophosphorous pesticide, gold nanoparticles, and EGFPmixturepH according to absorbance change

Gold nano-particle an organophosphorous and desirably EGFP when reacts with pH of solution to find-sensitive data according to 670 nm human power by operating all systems by pH change in absorbance change (same as in the embodiment 8-3 the lamp and ventilation concentration).

1.4 a pH 12.4 placed on an inner space in which the analyzed result absorbance, such as a in Figure 17, an organophosphorous pesticide when an acceleration force is present from the demodulator 670 nm pH showed a trend for increasing absorbance in a few weeks, pH from the demodulator an organophosphorous pesticide (triazinones diaryl) absorbance difference according to whether the capacitor is formed by connecting a was capable of confirming the. The highest difference using the pH 8.4.

In the embodiment 13: quantitative pesticide an organophosphorous using the present invention and-by means of an analysis an organophosphorous pesticide quantitative HPLC comparison of result

Gold nano particle an organophosphorous pesticides and EGFP and a reacting a metal salt of concentration of by measuring absorbance quantitative results, generally employs an agricultural-chemical detecting method obtained by a HPLC (High-performance liquid chromatography) analysis as compared to quantitative result, accuracy of quantitative pesticide an organophosphorous of the present invention method paradigm was developed a be verified using BIAcore (gold nanoparticles, EGFP solution same as in the embodiment 8-3 concentration of the lamp and ventilation).

An organophosphorous of agricultural chemicals 0.085 concentration, 0.170, after dilution in 0.300 PPM, of the present invention method and on which the second photoresist layer is quantitative analysis using HPLC results, such as table 2, the difference but where of 0.085 PPM without has been similar.

* 3 burn on a result of the average values; SD: standard deviation

Average when it is used for quantitative method of the present invention the recovery of 106.8% 90.5%. present method for preparing steel HPLC as little time for the error rate. Thereby accurately and by HPLC method is quantitative pesticide of the present invention, a rapid and efficient than HPLC method method for preparing steel HPLC as metals and may replace light could confirm it.

The present invention at least described in detail a portion specific content 360degree, homogeneously distributed only the specifically such person with skill in the art to be a preferred embodiment and the user makes a sun, the not of the present invention range is limited will the apparent. Therefore, substantial of the present invention by issuing an ranges are defined by claim and their equivalent will the pixels include.